LOCUS       NM_001005560            3100 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus phospholipase A2 group VI (Pla2g6), transcript
            variant 1, mRNA.
ACCESSION   NM_001005560 NM_053892 XM_346803
VERSION     NM_001005560.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Kuwata,H., Yuzurihara,C., Kinoshita,N., Taki,Y., Ikegami,Y.,
            Washio,S., Hirakawa,Y., Yoda,E., Aiuchi,T., Itabe,H., Nakatani,Y.
            and Hara,S.
  TITLE     The group VIA calcium-independent phospholipase A2 and NFATc4
            pathway mediates IL-1beta-induced expression of chemokines CCL2 and
            CXCL10 in rat fibroblasts
  JOURNAL   FEBS J 285 (11), 2056-2070 (2018)
   PUBMED   29637744
  REMARK    GeneRIF: the transcriptional activation of nuclear factor of
            activated T-cell proteins (NFATs), but not nuclear factor-kappaB,
            by IL-1beta stimulation was markedly attenuated by the iPLA2
            inhibitor bromoenol lactone, and NFATc4 knockdown markedly
            attenuated the IL-1beta-induced expression of both CCL2 and CXCL10.
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Zhou,Z., Wang,Z., Guan,Q., Qiu,F., Li,Y., Liu,Z., Zhang,H., Dong,H.
            and Zhang,Z.
  TITLE     PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia
            Cardiomyocytes through PEDF Receptor/Phospholipase A2
  JOURNAL   Int J Mol Sci 17 (12), 2064 (2016)
   PUBMED   27973457
  REMARK    GeneRIF: PEDF can protect against hypoxia-induced activation of the
            NLRP3 inflammasome by inhibiting mitochondrial fission though
            PEDFR/iPLA2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3100)
  AUTHORS   Caporarello,N., Salmeri,M., Scalia,M., Motta,C., Parrino,C.,
            Frittitta,L., Olivieri,M., Toscano,M.A., Anfuso,C.D. and Lupo,G.
  TITLE     Role of cytosolic and calcium independent phospholipases A(2) in
            insulin secretion impairment of INS-1E cells infected by S. aureus
  JOURNAL   FEBS Lett 589 (24 Pt B), 3969-3976 (2015)
   PUBMED   26632509
  REMARK    GeneRIF: Data suggest glucose-induced insulin secretion is first
            up-regulated (acute infection), then down-regulated (chronic
            infection) in beta cells infected with S. aureus; Pla2g4a is
            up-regulated in acute infection; Pla2g6 is up-regulated in chronic
            infection.
REFERENCE   4  (bases 1 to 3100)
  AUTHORS   Rauckhorst,A.J., Pfeiffer,D.R. and Broekemeier,K.M.
  TITLE     The iPLA(2)gamma is identified as the membrane potential sensitive
            phospholipase in liver mitochondria
  JOURNAL   FEBS Lett 589 (18), 2367-2371 (2015)
   PUBMED   26206229
  REMARK    GeneRIF: membrane potential sensitive activity is the iPLA2gamma
REFERENCE   5  (bases 1 to 3100)
  AUTHORS   Takatani-Nakase,T. and Takahashi,K.
  TITLE     Leptin suppresses non-apoptotic cell death in ischemic rat
            cardiomyocytes by reduction of iPLA(2) activity
  JOURNAL   Biochem Biophys Res Commun 463 (1-2), 13-17 (2015)
   PUBMED   25979360
  REMARK    GeneRIF: Leptin was shown to significantly inhibit the activity of
            iPLA2, which is considered to play crucial roles in non-apoptotic
            cell death, resulting in effective prevention of ischemia-induced
            myocyte death.
REFERENCE   6  (bases 1 to 3100)
  AUTHORS   Jenkins,C.M., Han,X., Mancuso,D.J. and Gross,R.W.
  TITLE     Identification of calcium-independent phospholipase A2 (iPLA2)
            beta, and not iPLA2gamma, as the mediator of arginine
            vasopressin-induced arachidonic acid release in A-10 smooth muscle
            cells. Enantioselective mechanism-based discrimination of mammalian
            iPLA2s
  JOURNAL   J Biol Chem 277 (36), 32807-32814 (2002)
   PUBMED   12089145
  REMARK    GeneRIF: The agonist-stimulated release of arachidonic acid (AA)
            from cellular phospholipids in many cell types (e.g. myocytes,
            beta-cells, and neurons) has been demonstrated to be primarily
            mediated by calcium-independent phospholipases A(2) (iPLA(2)s).
REFERENCE   7  (bases 1 to 3100)
  AUTHORS   Seegers,H.C., Gross,R.W. and Boyle,W.A.
  TITLE     Calcium-independent phospholipase A(2)-derived arachidonic acid is
            essential for endothelium-dependent relaxation by acetylcholine
  JOURNAL   J Pharmacol Exp Ther 302 (3), 918-923 (2002)
   PUBMED   12183647
REFERENCE   8  (bases 1 to 3100)
  AUTHORS   Bloch-Shilderman,E., Abu-Raya,S., Trembovler,V., Boschwitz,H.,
            Gruzman,A., Linial,M. and Lazarovici,P.
  TITLE     Pardaxin stimulation of phospholipases A2 and their involvement in
            exocytosis in PC-12 cells
  JOURNAL   J Pharmacol Exp Ther 301 (3), 953-962 (2002)
   PUBMED   12023524
REFERENCE   9  (bases 1 to 3100)
  AUTHORS   Ma,Z., Ramanadham,S., Wohltmann,M., Bohrer,A., Hsu,F.F. and Turk,J.
  TITLE     Studies of insulin secretory responses and of arachidonic acid
            incorporation into phospholipids of stably transfected insulinoma
            cells that overexpress group VIA phospholipase A2 (iPLA2beta)
            indicate a signaling rather than a housekeeping role for iPLA2beta
  JOURNAL   J Biol Chem 276 (16), 13198-13208 (2001)
   PUBMED   11278673
REFERENCE   10 (bases 1 to 3100)
  AUTHORS   Ma,Z., Ramanadham,S., Kempe,K., Chi,X.S., Ladenson,J. and Turk,J.
  TITLE     Pancreatic islets express a Ca2+-independent phospholipase A2
            enzyme that contains a repeated structural motif homologous to the
            integral membrane protein binding domain of ankyrin
  JOURNAL   J Biol Chem 272 (17), 11118-11127 (1997)
   PUBMED   9111008
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC081916.1.
            
            On or before Oct 14, 2004 this sequence version replaced
            NM_053892.1, XM_346803.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081916.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..3100
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /note="phospholipase A2 group VI"
                     /db_xref="GeneID:360426"
                     /db_xref="RGD:628867"
     exon            1..52
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            53..302
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..2517
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.4"
                     /EC_number="3.1.2.2"
                     /note="isoform 1 is encoded by transcript variant 1; 85
                     kDa calcium-independent phospholipase A2; patatin-like
                     phospholipase domain-containing protein 9; intracellular
                     membrane-associated calcium-independent phospholipase A2
                     beta; 85/88 kDa calcium-independent phospholipase A2;
                     phospholipase A2, group VI (cytosolic,
                     calcium-independent); palmitoyl-CoA hydrolase;
                     2-lysophosphatidylcholine acylhydrolase"
                     /codon_start=1
                     /product="85/88 kDa calcium-independent phospholipase A2
                     isoform 1"
                     /protein_id="NP_001005560.1"
                     /db_xref="GeneID:360426"
                     /db_xref="RGD:628867"
                     /translation="MQFFGRLVNTLSSVTNLFSNPFRVKEVSLADYASSERVREEGQL
                     ILLQNASNRTWDCVLVSPRNPQSGFRLFQLESEADALVNFQQYSSQLPPFYESSVQVL
                     HVEVLQHLTDLIRNHPSWTVTHLAVELGIRECFHHSRIISCANSTENEEGCTPLHLAC
                     RKGDSEILVELVQYCHAQMDVTDNKGETAFHYAVQGDNPQVLQLLGKNASAGLNQVNN
                     QGLTPLHLACQMGKQEMVRVLLLCNARCNIMGPGGFPIHTAMKFSQKGCAEMIISMDS
                     NQIHSKDPRYGASPLHWAKNAEMARMLLKRGCDVDSTSASGNTALHVAVTRNRFDCVM
                     VLLTYGANAGARGEHGNTPLHLAMSKDNMEMVKALIVFGAEVDTPNDFGETPAFIASK
                     ISKLITRKALLTLLKTVGADYHFPFIQGVSTEQSSAAGPHPFFSLDRTQPPTISLNNL
                     ELQDLMPVSRARKPAFILSSMRDEKRSHDHLLCLDGGGVKGLVIIQLLIAIEKASGVA
                     TKDLFDWVAGTSTGGILALAILHSKSMAYMRGVYFRMKDEVFRGSRPYESGPLEEFLK
                     REFGEHTKMTDVKKPKVMLTGTLSDRQPAELHLFRNYDAPEAVREPRCTPNINLKPPT
                     QPADQLVWRAARSSGAAPTYFRPNGRFLDGGLLANNPTLDAMTEIHEYNQDMIRKGQG
                     NKVKKLSIVVSLGTGKSPQVPVTCVDVFRPSNPWELAKTVFGAKELGKMVVDCCTDPD
                     GRAVDRARAWCEMVGIQYFRLNPQLGSDIMLDEVSDAVLVNALWETEVYIYEHREEFQ
                     KLVQLLLSP"
     exon            303..518
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            519..702
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            703..890
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            891..987
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1170
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1279
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1444
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1523
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1687
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1838
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1975
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2130
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2298
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2372
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..3077
                     /gene="Pla2g6"
                     /gene_synonym="caI-PLA2; GVI PLA2; iPla2; iPLA2-beta;
                     PNPLA9"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgcgtttgt ttgcggaagt taggagtgct gagaagtgct gagtaagctg agagcacagg
       61 ggcaggctgg gtcgcctacc tgtcccacat aggatgcagt tctttggacg cctcgttaac
      121 accctcagta gtgtcaccaa cttgttctca aacccattcc gggtaaagga ggtgtccttg
      181 gctgactatg cctcaagtga acgtgttcga gaggaagggc agctgatcct gttgcagaat
      241 gcttccaatc gtacctggga ctgtgtcctg gtcagcccta ggaacccaca gagtggcttc
      301 cgactcttcc aactggagtc agaggcagac gccctggtga acttccagca gtattcctcc
      361 cagctgccac cgttctatga gagctctgtg caggtcctgc atgtggaggt gctgcagcac
      421 ctgacggacc tcattcggaa ccaccccagc tggacagtga cgcacctggc cgtggaactt
      481 ggcattcggg agtgtttcca tcacagccgc atcatcagct gcgccaacag cacagagaat
      541 gaggagggct gtaccccact acatctggcc tgccgcaagg gtgacagtga gattctggtg
      601 gagttggtac agtactgcca cgcccagatg gatgtcactg acaacaaagg agagaccgcc
      661 ttccattatg ctgtgcaagg ggacaatccc caggtgctac agctcctagg aaagaatgcc
      721 tcagccggcc tgaaccaggt gaacaaccaa gggctgacgc cgctgcacct ggcctgccag
      781 atggggaagc aggagatggt gcgtgtcctg ctgctctgca atgcccgctg caacatcatg
      841 gggcccggtg gcttccccat ccacacagcc atgaagtttt cccagaaggg gtgtgctgaa
      901 atgattatca gcatggacag taaccagatc cacagcaagg atcctcgcta tggagccagc
      961 ccgctccact gggccaagaa cgctgagatg gcccgaatgc ttctgaagag gggctgtgac
     1021 gtggacagca caagtgcctc ggggaacaca gccctgcacg tggcggtgac gcgcaaccgc
     1081 tttgactgtg tcatggtgct gctgacctat ggggccaacg caggtgcccg aggagagcat
     1141 gggaacacac cactgcatct ggccatgtcg aaagataaca tggagatggt caaagccctc
     1201 attgtatttg gggcagaagt agacaccccg aatgactttg gggagactcc tgcattcata
     1261 gcttccaaga tcagcaagct gattaccagg aaggcgctct tgactctgct gaaaacggtg
     1321 ggggccgact accacttccc attcatacaa ggggtctcca cggagcagag ctctgcagct
     1381 ggacctcacc ccttcttctc cctggacaga actcagcctc caacgatcag cttaaacaac
     1441 ctagagcttc aggacctcat gcccgtctcc cgagcccgga agccagcgtt catcctgagc
     1501 tccatgaggg atgagaagcg aagccatgac cacctgctct gcttggatgg agggggcgtg
     1561 aagggcctgg tcatcatcca gcttctcatt gccattgaga aggcctcagg tgtggctacc
     1621 aaggacctct tcgactgggt ggcaggaacc agcacaggcg gcatcctggc cctggccatt
     1681 ctacacagta aatccatggc ctatatgcgt ggcgtgtact tccgtatgaa ggatgaggtg
     1741 tttcggggct cacggcccta tgagtctggg cccctggagg agttcctgaa gcgtgagttt
     1801 ggggagcaca ccaagatgac agatgtcaag aaacccaagg tgatgctgac agggacactg
     1861 tcagaccggc agccagcaga gctccacctc ttccggaatt acgacgcccc agaggccgtt
     1921 cgggaacctc gctgtacccc aaacattaac ctgaagccgc ccacccagcc tgcagaccag
     1981 ttagtatggc gggcagcccg gagcagtggg gcagccccaa cctatttccg gcccaatgga
     2041 cgcttcttgg atggtgggct gctggccaac aaccccacac tggatgccat gactgaaatc
     2101 catgagtaca atcaggatat gatccgcaag ggccagggca acaaggtgaa gaaactctcc
     2161 atagtcgtgt ctctggggac aggaaagtcc cctcaagtgc ctgtaacctg tgtagatgtc
     2221 tttcgtccca gcaacccttg ggaactggcc aagactgttt ttggagccaa ggaactgggc
     2281 aagatggtgg tggactgttg cacagatcca gatgggcggg ctgtggatcg tgcccgggcc
     2341 tggtgtgaga tggtcggtat ccagtacttc aggctgaacc cccagctagg ctcagacatc
     2401 atgctggacg aggtcagtga tgcagtgctg gtcaacgccc tctgggagac cgaggtctac
     2461 atctatgagc accgggagga gttccagaag cttgtccagc tgctgctatc tccctgagcc
     2521 cggccctgct ggcaggagtg cgccgggccg cctggcacac tgcgggccag gctggcctgc
     2581 cgctccatgt ccatctgaga ctagggctcg gagcaaacag gttccccatg aggcacctct
     2641 cctgatccat ccgcacttta ctactccagg ctgaaagccc agagttccct cggccccttc
     2701 ccctgactat gaaggacaac tgattccatc tactacccca tatatcagag atcaacacta
     2761 aggggccagt gtgcccagag ggccctccca ggctccctgg ccactgctgc ccaccccctc
     2821 tcccacttcc tgatgtcagg gattagagaa atggggttcc accccgtcat caggtgaaat
     2881 ccaggctaat gaaacccagc gccattccgc cattcgactt tgcccctcgc acctgcagcc
     2941 tctccaccct gcagccgccc cacaaggagc gctcctagct cccaaaggtc aatcctgtgc
     3001 acatgctctt ctctggaagg agagtgggga ggggctcaag gccacctcaa ctgtgaaata
     3061 aacggtttta cactcaaaaa aaaaaaaaaa aaaaaaaaaa
//
