LOCUS       NM_001105755            5592 bp    mRNA    linear   ROD 08-APR-2024
DEFINITION  Rattus norvegicus protein kinase N2 (Pkn2), mRNA.
ACCESSION   NM_001105755 XM_001076809 XM_215718
VERSION     NM_001105755.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   3  (bases 1 to 5592)
  AUTHORS   Wallace,S.W., Magalhaes,A. and Hall,A.
  TITLE     The Rho target PRK2 regulates apical junction formation in human
            bronchial epithelial cells
  JOURNAL   Mol Cell Biol 31 (1), 81-91 (2011)
   PUBMED   20974804
REFERENCE   4  (bases 1 to 5592)
  AUTHORS   Lachmann,S., Jevons,A., De Rycker,M., Casamassima,A., Radtke,S.,
            Collazos,A. and Parker,P.J.
  TITLE     Regulatory domain selectivity in the cell-type specific
            PKN-dependence of cell migration
  JOURNAL   PLoS One 6 (7), e21732 (2011)
   PUBMED   21754995
REFERENCE   5  (bases 1 to 5592)
  AUTHORS   Harrison,B.C., Huynh,K., Lundgaard,G.L., Helmke,S.M., Perryman,M.B.
            and McKinsey,T.A.
  TITLE     Protein kinase C-related kinase targets nuclear localization
            signals in a subset of class IIa histone deacetylases
  JOURNAL   FEBS Lett 584 (6), 1103-1110 (2010)
   PUBMED   20188095
REFERENCE   6  (bases 1 to 5592)
  AUTHORS   Kim,S.J., Kim,J.H., Kim,Y.G., Lim,H.S. and Oh,J.W.
  TITLE     Protein kinase C-related kinase 2 regulates hepatitis C virus RNA
            polymerase function by phosphorylation
  JOURNAL   J Biol Chem 279 (48), 50031-50041 (2004)
   PUBMED   15364941
REFERENCE   7  (bases 1 to 5592)
  AUTHORS   Torbett,N.E., Casamassima,A. and Parker,P.J.
  TITLE     Hyperosmotic-induced protein kinase N 1 activation in a vesicular
            compartment is dependent upon Rac1 and 3-phosphoinositide-dependent
            kinase 1
  JOURNAL   J Biol Chem 278 (34), 32344-32351 (2003)
   PUBMED   12783890
REFERENCE   8  (bases 1 to 5592)
  AUTHORS   Gross,C., Heumann,R. and Erdmann,K.S.
  TITLE     The protein kinase C-related kinase PRK2 interacts with the protein
            tyrosine phosphatase PTP-BL via a novel PDZ domain binding motif
  JOURNAL   FEBS Lett 496 (2-3), 101-104 (2001)
   PUBMED   11356191
REFERENCE   9  (bases 1 to 5592)
  AUTHORS   Yu,W., Liu,J., Morrice,N.A. and Wettenhall,R.E.
  TITLE     Isolation and characterization of a structural homologue of human
            PRK2 from rat liver. Distinguishing substrate and lipid activator
            specificities
  JOURNAL   J Biol Chem 272 (15), 10030-10034 (1997)
   PUBMED   9092545
REFERENCE   10 (bases 1 to 5592)
  AUTHORS   Morrice,N.A., Gabrielli,B., Kemp,B.E. and Wettenhall,R.E.
  TITLE     A cardiolipin-activated protein kinase from rat liver structurally
            distinct from the protein kinases C
  JOURNAL   J Biol Chem 269 (31), 20040-20046 (1994)
   PUBMED   8051089
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On May 29, 2008 this sequence version replaced NM_001105755.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643285.10307.1,
                                           SRR26643299.12362.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               JAXUCZ010000002.1  234558966-234559369 c
            405-705             JAXUCZ010000002.1  234530102-234530402 c
            706-860             JAXUCZ010000002.1  234507649-234507803 c
            861-978             JAXUCZ010000002.1  234499329-234499446 c
            979-1124            JAXUCZ010000002.1  234498008-234498153 c
            1125-1338           JAXUCZ010000002.1  234497704-234497917 c
            1339-1524           JAXUCZ010000002.1  234489463-234489648 c
            1525-1634           JAXUCZ010000002.1  234488527-234488636 c
            1635-1778           JAXUCZ010000002.1  234478580-234478723 c
            1779-1854           JAXUCZ010000002.1  234478222-234478297 c
            1855-2029           JAXUCZ010000002.1  234477371-234477545 c
            2030-2156           JAXUCZ010000002.1  234477031-234477157 c
            2157-2287           JAXUCZ010000002.1  234476507-234476637 c
            2288-2363           JAXUCZ010000002.1  234475038-234475113 c
            2364-2455           JAXUCZ010000002.1  234474870-234474961 c
            2456-2632           JAXUCZ010000002.1  234469530-234469706 c
            2633-2695           JAXUCZ010000002.1  234468031-234468093 c
            2696-2772           JAXUCZ010000002.1  234462359-234462435 c
            2773-2915           JAXUCZ010000002.1  234461460-234461602 c
            2916-3023           JAXUCZ010000002.1  234456862-234456969 c
            3024-3104           JAXUCZ010000002.1  234456576-234456656 c
            3105-5592           JAXUCZ010000002.1  234454001-234456488 c
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q44"
     gene            1..5592
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /note="protein kinase N2"
                     /db_xref="GeneID:207122"
                     /db_xref="RGD:620146"
     exon            1..404
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             357..3308
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /EC_number="2.7.11.13"
                     /note="protease-activated kinase 2; protein-kinase
                     C-related kinase 2; cardiolipin-activated protein kinase
                     Pak2; protein kinase C-like 2; PKN gamma; p140 kinase"
                     /codon_start=1
                     /product="serine/threonine-protein kinase N2"
                     /protein_id="NP_001099225.2"
                     /db_xref="GeneID:207122"
                     /db_xref="RGD:620146"
                     /translation="MASNPDRGEILLTELQGDSRPLPFSENVSAVQKLDFSDTIVQQK
                     LDDVKDRIKREIRKELKIKEGAENLRKVTTDKKNLAYVDNILKKSNKKLEELHHKLQE
                     LNAHIVVSDPEDSADCPRTPDTPNSDSRSSTSNNRLMALQKQLDIELKVKQGAENMIQ
                     MYSNGPSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELR
                     MEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLE
                     QRLNELPKNHPKSSVVIEELSLVASPTLSPRQSMLSTQNQYSTLSKPAALTGTLEVRL
                     MGCQDILENVPGRSKATSVALPGWSPSENRSSFMSRTSKSKSGSSRNLLKTDDLSNDV
                     CAVLKLDNTVVGQTIWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRL
                     EDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQ
                     MNINIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDARTPELAPPASDSTVTKLD
                     FDLEPEAPPAPPRASSLGEIDDSSELRVLDIPGQGSETVFDIENDRNNMRPKSKSEYE
                     LNIPDSSRSCWSVGELEDKRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKHTNEMF
                     AIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAA
                     GGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIA
                     DFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPF
                     PGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVKKHPF
                     FRLTDWSALLDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEM
                     FRDFDYVADWC"
     exon            405..705
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            706..860
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            861..978
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1124
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1338
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1524
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1634
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1778
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1854
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..2029
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2156
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2287
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2363
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2455
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2632
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2695
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2696..2772
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2915
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2916..3023
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3024..3104
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3105..5592
                     /gene="Pkn2"
                     /gene_synonym="Pak-2; Prkcl2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttttgcgaca cggtgacagc caaatggtgc gacaggagga gctgccgccg cggccgctgc
       61 gccccgcacc gagagctctg aggcggctcc cggcgtcgcc ctgagggagc ggctgaggga
      121 acagagtccg gcgggcggcg gcgggcggcg aggggagccg cgccgagggc cctcgctccc
      181 cgccgctctc catgagccgg gccgcctgag ccgcgcctgc agccgggccg cgcctccatg
      241 actccctagt tgctgctttc tctccggtcc tcaccccccc gcccggctcc cgtcccgcct
      301 tctccctcgg ccgcgccgct ggccgctttc aggtgcggag tcccgagcgg agcgccatgg
      361 cgtccaaccc cgaccggggg gagatcctcc tcacggagct gcagggagat tcccgacctc
      421 ttccattttc tgagaatgtg agtgctgttc agaaattaga cttttcagat acaatagtgc
      481 aacagaaatt ggatgacgtc aaggatcgaa taaagagaga aataaggaaa gaactgaaaa
      541 tcaaagaagg agctgaaaac ctgaggaaag ttacaacaga taagaaaaat ttggcttatg
      601 tagacaacat tttgaaaaag tcaaataaaa aattagaaga attacatcac aagctacaag
      661 aattaaatgc acacattgtt gtatcggatc cagaggactc tgcagattgt ccgaggactc
      721 cagatactcc aaatagtgac tctcgtagtt ctactagcaa caatagattg atggccttac
      781 agaaacaact ggatatagaa cttaaagtaa agcaaggtgc agagaacatg atacagatgt
      841 actcaaatgg cccttcaaag gatcggaaac tccatggtac agctcagcaa ctgctccagg
      901 atagcaagac aaaaatagaa gtcatccgaa tgcagattct tcaggccgtc cagaccaatg
      961 agctggcttt tgataatgca aaacctgtga taagccctct tgagcttcga atggaagaat
     1021 taagacatca ttttaggata gaatttgcag tagccgaagg tgcaaagaat gtcatgaaat
     1081 tacttggctc aggaaaagta acagacagaa aagcgctttc agaagcccaa gcaagattta
     1141 atgaatcaag tcagaagttg gaccttttaa aatattcact agagcagagg ttaaatgaac
     1201 tccccaagaa tcaccccaaa agcagtgtcg tcattgagga gctctccctt gttgcatcgc
     1261 caaccctgag tccacgacag agtatgctgt ccacgcagaa ccagtacagc acactgtcca
     1321 agccagcagc actaaccggt actttggaag ttcgtctcat ggggtgccaa gatatcctgg
     1381 agaatgtccc tggacggtca aaagcgacat ccgtagcact gcctggatgg agtccaagtg
     1441 aaaacagatc atcgtttatg agcagaacta gtaaaagtaa aagtggaagc agtcgcaacc
     1501 tcctgaaaac cgacgacttg tccaatgatg tttgtgctgt tttgaagcta gataatactg
     1561 tggttggtca aactatctgg aagcccattt ccaatcagtc gtgggaccag aagtttacac
     1621 tggaactaga caggtcacgt gaactggaaa tttcagttta ttggcgtgat tggagatctc
     1681 tgtgtgctgt aaaatttctg aggttagaag attttttaga caaccagcgg catggcatgt
     1741 gtctctattt ggaaccacag ggtactttgt ttgcagaggt tacttttttt aatccagtta
     1801 ttgaaagacg accaaaactc caaagacaaa agaaaatttt ttcaaaacaa caaggcaaaa
     1861 catttctcag agcccctcaa atgaatatta atattgccac ttggggaagg ctagtgagac
     1921 gagctattcc cacagtaaat cattctggca cgttcagccc tcagactcct gtgcccgcta
     1981 cagtgccagt ggtggatgca cgcactcctg aactcgcacc tccagccagt gattctacag
     2041 taaccaaatt ggactttgat cttgaacctg aagctcctcc agccccacca cgtgcttctt
     2101 cccttggaga gatagatgac tcttctgaat tgagagtttt ggatatacct ggacagggtt
     2161 cagaaactgt tttcgatatt gagaatgaca gaaataacat gcgtccaaaa tccaaatctg
     2221 aatatgagct taacattcct gactcaagtc gaagctgttg gagtgttgga gaacttgagg
     2281 acaagagatc tcaacagagg tttcagttta atctacaaga cttcaggtgc tgtgctgtct
     2341 tggggagagg acactttgga aaggtgctgt tggcagaata taaacacaca aatgaaatgt
     2401 ttgcaataaa agccttaaag aaaggggaca ttgtggcacg agatgaagta gacagcttga
     2461 tgtgtgaaaa aagaattttt gagactgtga acagtgtccg gcatcctttc ctagtgaacc
     2521 tgtttgcatg tttccaaacc aaagagcatg tttgctttgt gatggagtac gctgctggtg
     2581 gggacctgat gatgcacatc cacactgacg tcttctctga acccagagct gtattttatg
     2641 cagcatgtgt agttcttgga ttgcaatatt tacatgaaca caaaattgtt tatagagact
     2701 tgaagttgga taacctgttg ctggatacag aaggcttcgt gaagattgct gactttggtc
     2761 tttgcaaaga aggaatgggg tatggagata ggacaagcac attttgtggt actcctgaat
     2821 ttcttgcccc agaagtatta acagaaacgt cgtacacaag ggctgtagat tggtggggcc
     2881 ttggcgtact tatatatgaa atgctcgtcg gagagtctcc ctttcctggt gatgatgaag
     2941 aagaggtttt tgacagtatt gtaaatgacg aagtaaggta tccaaggttc ttatctacag
     3001 aagccatttc cataatgaga aggctgttga ggaggaatcc tgagcggcgc ctgggagctg
     3061 gtgagaaaga tgccgaggat gtaaagaagc acccgttttt ccggctaacc gactggagtg
     3121 cactactgga caagaaagta aagccaccat ttgtacctac cattagagga cgggaagatg
     3181 ttagtaactt tgatgatgaa tttacctcag aagcacctat tctgactccc cctcgagaac
     3241 cgaggatact cttggaggag gagcaggaga tgttccgcga ttttgactac gttgctgatt
     3301 ggtgttaagt ttctggacac ggagaccaag cggactgaca gacaagaaga cctctagaga
     3361 acagcagcct ttcatttgct ttccgtgccc aggagctttt gaggccatat aaagcacatg
     3421 aagatgtggt tacagcactg ttcatacctt cttaatgttt tcaacggaaa tctgaacact
     3481 ggaagcagtt tcacggagtt taggctgagt gaacattggc catggaaatc cattacaaat
     3541 aaatactttt acatcaatac tctattttta aaaagaagag gaaccactct tttatagtcc
     3601 ccatctttgc cctagcctgc cttaagtgga aggaagatta atcacttaac tgtattttac
     3661 aaaagaaaaa atagggcttt ggatgctatt actagtcata taaaaagtat gattctgttt
     3721 gactaaggca attattcttt aagaaatcac tataatatga aaaactgtgg cagtttgtac
     3781 acccttggtg gcttgatttt aagaaaaaaa tgagaatgta ctgatgtgta gtttgatgaa
     3841 ttttctgtat ttatcaggag aacacttcat tgccttaacg ttaccaacca tgcagcagag
     3901 cctcacggct tccagctgcg ctgcctgcca cacgagctgt gcttattaac agtgctgcag
     3961 aacgatagga gcgccagcgc ccactcacag tctcagccag gctctgggca gccgggacca
     4021 aggtaccgtg ctgactgtgt gcactcccca agtccaggcc ccacggagga tgttctgtcg
     4081 ggtcttccca agtcagagcc cgatggaccc ttatcattct atgcattcta cacggatttc
     4141 aaccgagaca tcttgttctt gtgtctgctg taggagggaa ctctatcttt gttaaaaaaa
     4201 ccatagagat tatcatggta tacatgctgt gaacatgtat atgtacaagt tgtaatgtat
     4261 tctgtgttgt aggcatatta tttaatttca ccaaatcatc acttttgtac agtggccaag
     4321 cagacacctc caactccagc atttctatta agtgcatttg tacattttag gccgctggat
     4381 ccagacttta tattggcagt tactgagggc aaaagcaata tattgtaaca gaatgtataa
     4441 atatttttga taaaacagtc tatattttat aaaaagttat tataacacta aagctgtacc
     4501 tcaaaagtct cacaaataat ttgatcaaat actctacaca tcctcagagg atccgtttgt
     4561 ggctttttat cgctctcatc atatgccgtt ttgcaaatca tgatcttcac tgggctggaa
     4621 attttttagg tttttgtttt gtttttctct tgcctgtcac tccattgttc tccctcagtt
     4681 cagtaatatc attgataaca ctgtgcttag catgttaggg ccatgatacc tcaggaacag
     4741 caagttggcc actaaccaca cttgaattaa ccatgtaatc acagtgagct catctcttac
     4801 aattgtccag atctgagtca atgagaaaca ggaaagcaca caaatacatg aagagttgag
     4861 cttgagacag ccttgctgag atacattggc ctgttgtcac tgtactctga gaataattaa
     4921 atttgtccag agatagttta taataccttc caaattcatt accatttttc tcccaagatc
     4981 ctttggaaga aattttctaa aaagaggagc aatatgttgc tgtaagttgc tttaaatgcc
     5041 agttaattgc cttggaaaag aacaatagaa aaaatagcat aattgtctag cttcaatttg
     5101 aacagtgtta acacaatctg aaaataaaac gctaactact tttgaggtac agtctgcata
     5161 cctttgttgg ttctgaaata tgcacagaaa gcagcgtttc aaatgggctt ttagcattaa
     5221 aaactgacag agttatatat acagtgtaca aaaacttttt taattgaggt caatcactaa
     5281 aaaaattagc gatcagggta tagtcacaca attatgctga aaaaaaaagc actcattttt
     5341 ctgtagttta aaaaatatat atattagcca gattttaaat tttaagagtg taaatagatg
     5401 ttgctattac tgtatgtgta ctggctgctc aagcactttg taaggagagc ggatacttta
     5461 gaatggtacc ctatgcatcc aaatgaaatg tgtgccttta cgtcattctg agaaagaggg
     5521 ggtttgcagt tataagtttc cacaaatgca caaattaaag ttcaaaatag attgaaattt
     5581 gtaactttgg tt
//
