LOCUS       NM_017175               3087 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus protein kinase N1 (Pkn1), mRNA.
ACCESSION   NM_017175
VERSION     NM_017175.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Wang,D., Shang,Q., Mao,J., Gao,C., Wang,J., Wang,D., Wang,H.,
            Jia,H., Peng,P., Du,M., Luo,Z. and Yang,L.
  TITLE     Phosphorylation of KRT8 (keratin 8) by excessive mechanical
            load-activated PKN (protein kinase N) impairs autophagosome
            initiation and contributes to disc degeneration
  JOURNAL   Autophagy 19 (9), 2485-2503 (2023)
   PUBMED   36897022
  REMARK    GeneRIF: Phosphorylation of KRT8 (keratin 8) by excessive
            mechanical load-activated PKN (protein kinase N) impairs
            autophagosome initiation and contributes to disc degeneration.
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Francois,A.A., Obasanjo-Blackshire,K., Clark,J.E., Boguslavskyi,A.,
            Holt,M.R., Parker,P.J., Marber,M.S. and Heads,R.J.
  TITLE     Loss of Protein Kinase Novel 1 (PKN1) is associated with mild
            systolic and diastolic contractile dysfunction, increased
            phospholamban Thr17 phosphorylation, and exacerbated
            ischaemia-reperfusion injury
  JOURNAL   Cardiovasc Res 114 (1), 138-157 (2018)
   PUBMED   29045568
  REMARK    GeneRIF: Cardioprotection by PKN1 is associated with reduced
            CamKIIdelta-dependent phospholamban Thr17 phosphorylation at the SR
            and therefore may stabilize the coupling of SR Ca2+ handling and
            contractile function, independent of its kinase activity.
REFERENCE   3  (bases 1 to 3087)
  AUTHORS   Yasui,T., Sakakibara-Yada,K., Nishimura,T., Morita,K., Tada,S.,
            Mosialos,G., Kieff,E. and Kikutani,H.
  TITLE     Protein kinase N1, a cell inhibitor of Akt kinase, has a central
            role in quality control of germinal center formation
  JOURNAL   Proc Natl Acad Sci U S A 109 (51), 21022-21027 (2012)
   PUBMED   23223530
REFERENCE   4  (bases 1 to 3087)
  AUTHORS   Singh,N.K., Kundumani-Sridharan,V., Kumar,S., Verma,S.K., Kotla,S.,
            Mukai,H., Heckle,M.R. and Rao,G.N.
  TITLE     Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin
            D1-CDK6 activity and modulates vascular smooth muscle cell division
            and migration leading to inward blood vessel wall remodeling
  JOURNAL   J Biol Chem 287 (43), 36291-36304 (2012)
   PUBMED   22893700
  REMARK    GeneRIF: Protein kinase N1 is a novel substrate of NFATc1-mediated
            cyclin D1-CDK6 activity and modulates vascular smooth muscle cell
            division and migration leading to inward blood vessel wall
            remodeling.
REFERENCE   5  (bases 1 to 3087)
  AUTHORS   Lachmann,S., Jevons,A., De Rycker,M., Casamassima,A., Radtke,S.,
            Collazos,A. and Parker,P.J.
  TITLE     Regulatory domain selectivity in the cell-type specific
            PKN-dependence of cell migration
  JOURNAL   PLoS One 6 (7), e21732 (2011)
   PUBMED   21754995
REFERENCE   6  (bases 1 to 3087)
  AUTHORS   Maesaki,R., Ihara,K., Shimizu,T., Kuroda,S., Kaibuchi,K. and
            Hakoshima,T.
  TITLE     The structural basis of Rho effector recognition revealed by the
            crystal structure of human RhoA complexed with the effector domain
            of PKN/PRK1
  JOURNAL   Mol Cell 4 (5), 793-803 (1999)
   PUBMED   10619026
REFERENCE   7  (bases 1 to 3087)
  AUTHORS   Mellor,H., Flynn,P., Nobes,C.D., Hall,A. and Parker,P.J.
  TITLE     PRK1 is targeted to endosomes by the small GTPase, RhoB
  JOURNAL   J Biol Chem 273 (9), 4811-4814 (1998)
   PUBMED   9478917
REFERENCE   8  (bases 1 to 3087)
  AUTHORS   Peng,B., Morrice,N.A., Groenen,L.C. and Wettenhall,R.E.
  TITLE     Phosphorylation events associated with different states of
            activation of a hepatic cardiolipin/protease-activated protein
            kinase. Structural identity to the protein kinase N-type protein
            kinases
  JOURNAL   J Biol Chem 271 (50), 32233-32240 (1996)
   PUBMED   8943281
REFERENCE   9  (bases 1 to 3087)
  AUTHORS   Morrice,N.A., Gabrielli,B., Kemp,B.E. and Wettenhall,R.E.
  TITLE     A cardiolipin-activated protein kinase from rat liver structurally
            distinct from the protein kinases C
  JOURNAL   J Biol Chem 269 (31), 20040-20046 (1994)
   PUBMED   8051089
REFERENCE   10 (bases 1 to 3087)
  AUTHORS   Mukai,H. and Ono,Y.
  TITLE     A novel protein kinase with leucine zipper-like sequences: its
            catalytic domain is highly homologous to that of protein kinase C
  JOURNAL   Biochem Biophys Res Commun 199 (2), 897-904 (1994)
   PUBMED   8135837
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB608722.1, D26180.1, BC061836.1 and EV774518.1.
            
            On Jan 24, 2008 this sequence version replaced NM_017175.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ222217.1, D26180.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132263, SAMD00132264
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                CB608722.1         1-57
            58-99               D26180.1           9-50
            100-1547            BC061836.1         1-1448
            1548-1703           D26180.1           1499-1654
            1704-1868           BC061836.1         1605-1769
            1869-2219           D26180.1           1820-2170
            2220-3078           BC061836.1         2121-2979
            3079-3087           EV774518.1         699-707
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q11"
     gene            1..3087
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /note="protein kinase N1"
                     /db_xref="GeneID:29355"
                     /db_xref="RGD:69308"
     exon            1..195
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..3015
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /EC_number="2.7.11.13"
                     /note="protein kinase C-like PKN; protease-activated
                     kinase 1; protein-kinase C-related kinase 1;
                     serine-threonine protein kinase N; protein kinase C-like
                     1; protein kinase PKN"
                     /codon_start=1
                     /product="serine/threonine-protein kinase N1"
                     /protein_id="NP_058871.2"
                     /db_xref="GeneID:29355"
                     /db_xref="RGD:69308"
                     /translation="MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKR
                     EIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPD
                     PTAGSDAPQSLAEGSPVCSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDR
                     KLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGQLESQAAPDEAHGDPDLGAVELRI
                     EELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLRESLER
                     RLGELPADHPKGRLLREELTAASSAAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVV
                     GCKNLPETIPWSPPPSVGASGTPDSRTPFLSRPARGLYNRSGSLSGRSSLKGEAENST
                     EVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFL
                     KLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGQTFQRA
                     RQMNIDVATWVRLLRRLIPNAVATGSFSPNASPGSEIRSTGDISMEKLNLGADSDSSS
                     QKSPAGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAV
                     LGRGHFGKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAGHPFL
                     VNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKI
                     VYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTR
                     AVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRR
                     NPERRLGSTERDAEDVKKQPFFRTLDWDALLARRLPPPFVPTLSGRTDVSNFDEEFTG
                     EAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY"
     exon            196..496
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            497..648
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            649..793
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            794..939
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1153
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1348
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1458
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1602
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1678
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1820
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1914
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..1994
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2070
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2162
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2339
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2340..2402
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2479
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2480..2622
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2730
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2811
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..3087
                     /gene="Pkn1"
                     /gene_synonym="PAK-1; Prkcl1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atcctgcagc cgctggggcc cgagggcgca gctcggcgga ggcctggcca ggccgggccg
       61 agcgcagcag gccggatcgc gcagcgcgcc agggaaggtc tgggctccgc gcttcccgcc
      121 ccacacgccg acgcgctcgc aagccctccc tcggcgctgg gacccctggc ggacatggcc
      181 ggcgacgccg tgcagagtga acctcgcagc tggtcactgc tggagcagct gggtctggct
      241 ggggcagacc tggcagcccc tggggtgcag cagcagctgg agttagagcg agagcggctg
      301 aagcgggaaa tccgaaaaga gctgaagctg aaggagggcg ctgagaacct gaggcgggcc
      361 accactgacc tgggccgcag cttggcccct gtggaactgc tgctgagggg ctccgctaga
      421 cggcttgact tgctgcacca gcagctgcag gagctgcatg cacatgtggt gctgcccgac
      481 cctacagcgg ggagtgatgc tccccaatcc cttgcagagg gcagccctgt ctgctcatcc
      541 accaacctga gccgagtggc tggcctggag aagcagctgg ccattgagct caaggtcaaa
      601 cagggggcag aaaacatgat ccagacctac agcaatggca gcaccaagga ccggaagctg
      661 ctgttgacgg cccaacagat gctgcaggat agtaagacca agattgacat catccgcatg
      721 cagcttcgcc gggcgctaca agcactccag gctggccagc tggagagtca ggcagctcct
      781 gatgaggccc acggagatcc agacctggga gccgtagagc tacgcattga ggagctacga
      841 caccattttc gagtagagca tgcagtggca gaaggcgcca agaatgtcct gcgtctgctc
      901 agtgctgcaa aggccccaga ccgcaaagca gtcagcgagg ctcaggagaa attgactgag
      961 tccaaccaga agctgggctt gctgcgggag tcactggaga ggcgcttggg ggaactgcct
     1021 gctgatcacc ccaagggacg cctgcttcgg gaggagctca ctgcggcctc atcggcagcc
     1081 ttcagtgcaa tactgcctgg gcccttccct gccactcact acagcacctt gagcaagcct
     1141 gcaccactca cagggaccct ggaagtacga gtggtgggct gcaaaaacct tcccgagacc
     1201 atcccttgga gccctccccc ctcagtcggg gcatctggga cccccgacag ccgcactcct
     1261 ttcctgagtc gtccagctcg gggcctttac aaccgaagtg gaagccttag tggacggagc
     1321 agcctcaagg gggaggcaga gaattccact gaggtcagca ccgtgctcaa gctggacaac
     1381 actgtggtgg ggcaaacagc ctggaagcca tgcggcccca atgcctggga ccagagcttc
     1441 accctggagc tggagagggc tcgggagctg gagttggctg tgttctggcg tgaccagagg
     1501 ggtctgtgtg ctctcaaatt tctgaagttg gaagacttct tggacaatga gaggcatgag
     1561 gtgcagctgg acatggaacc ccagggctgc ctggtggctg aggtcacctt ccgtaacccc
     1621 atcatcgagc ggatccctag gctccaaagg cagaaaaaaa ttttctccaa gcagcaaggg
     1681 cagacatttc agcgtgccag acagatgaac atcgatgtgg ccacctgggt gcggctgctc
     1741 cggagactca tcccgaacgc cgtggccact ggctccttca gccccaatgc atctccaggc
     1801 tctgagatcc ggagcactgg agacatatcc atggagaaat tgaatctcgg tgctgactca
     1861 gacagctcgt cccagaagag ccccgcaggg ctgccctcca cctcatgtag cctgagttct
     1921 ccaacccacg aatccaccac ctctccagag ctgccttcag agacccagga gaccccaggc
     1981 cctggcctgt gcagtcccct gaggaagtcg cccctgacgc ttgaggactt caagttcctg
     2041 gcagtgcttg gtcggggtca ctttggaaag gtgctgctgt ctgaattcca ctccagtggg
     2101 gagctctttg ccattaaagc cttgaagaaa ggtgacattg tagcccggga tgaggttgag
     2161 agcctgatgt gtgagaagcg gattttggcg accgtgacca gggcaggaca tcccttcctg
     2221 gtgaaccttt tcggctgttt ccagacccca gagcatgtgt gctttgtgat ggagtactca
     2281 gccggtgggg acttgatgct gcatatccac agcgacgtgt tctcagagcc tcgggctgtc
     2341 ttctattcgg cctgtgtggt gctgggactg cagttcctcc atgaacacaa gattgtctac
     2401 agggacctga agttggacaa tttgctcctg gatactgagg gctacgtcaa gatcgcagac
     2461 tttggcctct gcaaggaggg gatgggctat ggggaccgga ccagcacatt ctgcggaact
     2521 ccggagttcc tggcgccgga agtgctcaca gacacatcct acactcgagc cgtggactgg
     2581 tggggactgg gtgtattgct ctatgagatg ctggttggag agtctccgtt ccctggggac
     2641 gacgaggagg aagtatttga cagcatcgtc aatgatgagg ttcgttatcc ccgcttcctg
     2701 tctgcggagg ccatcggcat catgagaagg ctactgcgga ggaacccaga gcggaggttg
     2761 ggatccactg agcgtgatgc agaagatgtg aaaaaacagc ctttcttcag gactctggac
     2821 tgggatgccc tgctggcccg tcgcctgcct ccacccttcg tgcctacact ttcggggcgc
     2881 acagacgtca gcaacttcga tgaggagttc actggggagg cccccacact gagccctccc
     2941 cgggatgcac ggcccctgac agctgcggag caggcggcct tccgggattt cgactttgtg
     3001 gcaggaggct attagcccta agcccctgcc ttgcccaaga gttcttggtt tttaaaaaag
     3061 cctttggggt ttactccata catgcat
//
