LOCUS       NM_053297               1971 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus pyruvate kinase M1/2 (Pkm), mRNA.
ACCESSION   NM_053297
VERSION     NM_053297.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Sha,S., Jin,N., Zhou,R., Ruan,Y. and Ouyang,Y.
  TITLE     The Activation of PKM2 Induces Pyroptosis in Hippocampal Neurons
            via the NLRP3/Caspase-1/GSDMD Pathway in Neonatal Rats With
            Hypoxic-Ischemic Brain Injury
  JOURNAL   Brain Behav 14 (10), e70108 (2024)
   PUBMED   39444090
  REMARK    GeneRIF: The Activation of PKM2 Induces Pyroptosis in Hippocampal
            Neurons via the NLRP3/Caspase-1/GSDMD Pathway in Neonatal Rats With
            Hypoxic-Ischemic Brain Injury.
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Qian,J., Huang,C., Wang,M., Liu,Y., Zhao,Y., Li,M., Zhang,X.,
            Gao,X., Zhang,Y., Wang,Y., Huang,J., Li,J., Zhou,Q., Liu,R.,
            Wang,X., Cui,J. and Yang,Y.
  TITLE     Nuclear translocation of metabolic enzyme PKM2 participates in high
            glucose-promoted HCC metastasis by strengthening immunosuppressive
            environment
  JOURNAL   Redox Biol 71, 103103 (2024)
   PUBMED   38471282
  REMARK    GeneRIF: Nuclear translocation of metabolic enzyme PKM2
            participates in high glucose-promoted HCC metastasis by
            strengthening immunosuppressive environment.
REFERENCE   3  (bases 1 to 1971)
  AUTHORS   Cui,X., Jia,R., Zhao,R., Ma,N. and Jiang,F.
  TITLE     Silencing PKM2 Attenuates Brain Injury Induced by Status
            Epilepticus by Inhibiting the AKT/mTOR Pathway and the NLRP3
            Inflammasome
  JOURNAL   Neurochem Res 49 (1), 212-221 (2024)
   PUBMED   37702892
  REMARK    GeneRIF: Silencing PKM2 Attenuates Brain Injury Induced by Status
            Epilepticus by Inhibiting the AKT/mTOR Pathway and the NLRP3
            Inflammasome.
REFERENCE   4  (bases 1 to 1971)
  AUTHORS   Rihan,M. and Sharma,S.S.
  TITLE     Inhibition of Pyruvate kinase M2 (PKM2) by shikonin attenuates
            isoproterenol-induced acute myocardial infarction via reduction in
            inflammation, hypoxia, apoptosis, and fibrosis
  JOURNAL   Naunyn Schmiedebergs Arch Pharmacol 397 (1), 145-159 (2024)
   PUBMED   37382601
  REMARK    GeneRIF: Inhibition of Pyruvate kinase M2 (PKM2) by shikonin
            attenuates isoproterenol-induced acute myocardial infarction via
            reduction in inflammation, hypoxia, apoptosis, and fibrosis.
REFERENCE   5  (bases 1 to 1971)
  AUTHORS   Guo,L., Wang,L., Qin,G., Zhang,J., Peng,J., Li,L., Chen,X.,
            Wang,D., Qiu,J. and Wang,E.
  TITLE     M-type pyruvate kinase 2 (PKM2) tetramerization alleviates the
            progression of right ventricle failure by regulating oxidative
            stress and mitochondrial dynamics
  JOURNAL   J Transl Med 21 (1), 888 (2023)
   PUBMED   38062516
  REMARK    GeneRIF: M-type pyruvate kinase 2 (PKM2) tetramerization alleviates
            the progression of right ventricle failure by regulating oxidative
            stress and mitochondrial dynamics.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1971)
  AUTHORS   Parkison,C., Ashizawa,K., McPhie,P., Lin,K.H. and Cheng,S.Y.
  TITLE     The monomer of pyruvate kinase, subtype M1, is both a kinase and a
            cytosolic thyroid hormone binding protein
  JOURNAL   Biochem Biophys Res Commun 179 (1), 668-674 (1991)
   PUBMED   1883388
REFERENCE   7  (bases 1 to 1971)
  AUTHORS   Parkison,C., Kato,H. and Cheng,S.Y.
  TITLE     The nucleotide sequence of a full length cDNA encoding rat
            pituitary pyruvate kinase
  JOURNAL   Nucleic Acids Res 17 (17), 7106 (1989)
   PUBMED   2780321
REFERENCE   8  (bases 1 to 1971)
  AUTHORS   Takenaka,M., Noguchi,T., Inoue,H., Yamada,K., Matsuda,T. and
            Tanaka,T.
  TITLE     Rat pyruvate kinase M gene. Its complete structure and
            characterization of the 5'-flanking region
  JOURNAL   J Biol Chem 264 (4), 2363-2367 (1989)
   PUBMED   2914912
  REMARK    Erratum:[J Biol Chem 1989 Sep 25;264(27):16333]
REFERENCE   9  (bases 1 to 1971)
  AUTHORS   Noguchi,T., Inoue,H. and Tanaka,T.
  TITLE     The M1- and M2-type isozymes of rat pyruvate kinase are produced
            from the same gene by alternative RNA splicing
  JOURNAL   J Biol Chem 261 (29), 13807-13812 (1986)
   PUBMED   3020052
REFERENCE   10 (bases 1 to 1971)
  AUTHORS   Wagner,K.R., Carlson,B.M. and Max,S.R.
  TITLE     Developmental patterns of glycolytic enzymes in regenerating
            skeletal muscle after autogenous free grafting
  JOURNAL   J Neurol Sci 34 (3), 373-390 (1977)
   PUBMED   145474
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X15800.1.
            
            On Aug 31, 2012 this sequence version replaced NM_053297.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X15800.1, FQ223721.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1971              X15800.1           3-1973
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..1971
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /note="pyruvate kinase M1/2"
                     /db_xref="GeneID:25630"
                     /db_xref="RGD:3337"
     exon            1..69
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            70..236
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..1678
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /EC_number="2.7.1.40"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="pyruvate kinase isozymes M1/M2; pyruvate kinase
                     muscle isozyme; M2 pyruvate kinase; pyruvate kinase,
                     muscle; tyrosine-protein kinase PKM2; threonine-protein
                     kinase PKM2"
                     /codon_start=1
                     /product="pyruvate kinase PKM"
                     /protein_id="NP_445749.1"
                     /db_xref="GeneID:25630"
                     /db_xref="RGD:3337"
                     /translation="MPKPDSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARN
                     TGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRAATESFASDP
                     ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
                     YKNICKVVEVGSKIYVDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVNLPGAAVDL
                     PAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEG
                     VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
                     IKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAVFHR
                     LLFEELARASSQSTDPLEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPI
                     IAVTRNPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGKARGFFKKGDV
                     VIVLTGWRPGSGFTNTMRVVPVP"
     exon            237..328
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            329..460
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            461..647
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            648..918
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1069
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1222
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1389
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1571
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1971
                     /gene="Pkm"
                     /gene_synonym="PK; Pk3; PKM12; Pkm2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagcgaccc gtcctaagtc gacagacgtc ctctttaggt attgcaacag gatctgaagt
       61 acgcccgagg atctcagaaa ccatgcccaa gccagacagc gaagcaggga ctgccttcat
      121 tcagacccag cagctccatg cagccatggc tgacaccttc ctggaacaca tgtgccgcct
      181 ggacattgac tccgcaccca tcacggcccg caacactggg atcatctgta ccattggccc
      241 tgcttcccga tctgtggaga tgctgaagga gatgattaag tctgggatga atgtggctcg
      301 gctgaatttc tctcatggaa cccatgagta ccatgcagag actatcaaga atgtccgtgc
      361 agccacagaa agctttgcat ctgatcccat tctctaccga cctgttgcgg tggctctgga
      421 tacaaaggga cctgagatcc ggactggact catcaagggc agcggcaccg cagaggtgga
      481 gctgaagaag ggagccacac tgaagatcac cctggacaac gcctacatgg agaagtgcga
      541 tgagaacatc ctgtggctgg actataagaa catctgcaag gtggtggagg tgggcagcaa
      601 gatctacgtg gacgatgggc tcatctccct gcaggtgaag gagaaaggtg ctgactacct
      661 ggtgacagaa gtggaaaatg gtggctcctt gggcagcaag aagggcgtga acctgcctgg
      721 tgctgctgtg gacctccctg ctgtgtcaga aaaggacatc caggacctga agtttggggt
      781 ggagcaggac gtggacatgg tgtttgcgtc tttcatccgc aaggcggctg acgtgcatga
      841 ggttaggaag gtcctgggag agaagggcaa gaacatcaag atcatcagca aaatcgagaa
      901 ccatgaaggt gtccgcaggt ttgatgagat tttggaggcc agcgatggaa tcatggtagc
      961 tcgtggtgac ctgggcattg agattccggc agagaaggtc ttcctagctc agaagatgat
     1021 gattggacga tgcaaccgag ctgggaagcc agtcatctgc gccacccaga tgctggagag
     1081 catgatcaag aagccacgcc ccacccgtgc tgaaggcagt gacgtggcca atgcagtcct
     1141 agatggagct gactgcatca tgctgtccgg agaaacagcc aaaggggact accctctgga
     1201 ggctgttcgc atgcagcacc tgatagctcg agaggctgag gcagccgtgt tccaccgcct
     1261 gctgtttgaa gagcttgcgc gagcctccag tcaatccaca gaccccctgg aggccatggc
     1321 catgggcagc gtggaggcct cttataaatg tttagcagca gctttgatag ttctgacgga
     1381 gtccggcagg agtgctcacc aagtggcccg gtaccgccca agggctccta tcattgctgt
     1441 gacacgcaat ccccagacag cccgccaggc ccatctgtac cgtggcatct tccctgtgct
     1501 gtgtaaggat gccgtactgg atgcctgggc tgaggacgtt gatcttcgtg tgaacttggc
     1561 catgaatgtt ggcaaggccc gaggcttctt caagaaggga gatgtggtca ttgtgctgac
     1621 tggatggcgc cctggctctg gcttcaccaa caccatgcgt gtagtgcctg taccatgatg
     1681 atcctctgga gcttctcttc tagcccctgt cccttcccct cccctatcct atccattagg
     1741 ccagcaacgc ttgtagtgct cactctgggc catagtgtgg cgctggtggg ctgggacacc
     1801 aggaaaaatt aatgcctctg aaacatgcaa tagagcccag ctatttttca tggccctact
     1861 tgagccaggg gtgaaggagg aatgcaggat tggaaaccct ctgactttat cacagaaggg
     1921 cagcattatc tctgtgttct ttgctcctgt agaaagtttt ccagagaatt c
//
