LOCUS       NM_012624               2922 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus pyruvate kinase L/R (Pklr), mRNA.
ACCESSION   NM_012624
VERSION     NM_012624.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Gassaway,B.M., Cardone,R.L., Padyana,A.K., Petersen,M.C.,
            Judd,E.T., Hayes,S., Tong,S., Barber,K.W., Apostolidi,M.,
            Abulizi,A., Sheetz,J.B., Kshitiz, Aerni,H.R., Gross,S., Kung,C.,
            Samuel,V.T., Shulman,G.I., Kibbey,R.G. and Rinehart,J.
  TITLE     Distinct Hepatic PKA and CDK Signaling Pathways Control
            Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic
            Glucose Production
  JOURNAL   Cell Rep 29 (11), 3394-3404 (2019)
   PUBMED   31825824
  REMARK    GeneRIF: Distinct Hepatic PKA and CDK Signaling Pathways Control
            Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic
            Glucose Production.
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Canu,G., De Bonis,M., Minucci,A. and Capoluongo,E.
  TITLE     Red blood cell PK deficiency: An update of PK-LR gene mutation
            database
  JOURNAL   Blood Cells Mol Dis 57, 100-109 (2016)
   PUBMED   26832193
  REMARK    Review article
REFERENCE   3  (bases 1 to 2922)
  AUTHORS   Zhang,H., Li,Y., Hu,J., Shen,W.J., Singh,M., Hou,X., Bittner,A.,
            Bittner,S., Cortez,Y., Tabassum,J., Kraemer,F.B. and Azhar,S.
  TITLE     Effect of Creosote Bush-Derived NDGA on Expression of Genes
            Involved in Lipid Metabolism in Liver of High-Fructose Fed Rats:
            Relevance to NDGA Amelioration of Hypertriglyceridemia and Hepatic
            Steatosis
  JOURNAL   PLoS One 10 (9), e0138203 (2015)
   PUBMED   26394137
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2922)
  AUTHORS   Faustova,I., Loog,M. and Jarv,J.
  TITLE     Probing L-pyruvate kinase regulatory phosphorylation site by
            mutagenesis
  JOURNAL   Protein J 31 (7), 592-597 (2012)
   PUBMED   22878931
  REMARK    GeneRIF: Studied the effect of the point mutations around this
            phosphorylation site on the catalytic properties of L-pyruvate
            kinase.
REFERENCE   5  (bases 1 to 2922)
  AUTHORS   Jiang,M., Zhang,Y., Liu,M., Lan,M.S., Fei,J., Fan,W., Gao,X. and
            Lu,D.
  TITLE     Hypermethylation of hepatic glucokinase and L-type pyruvate kinase
            promoters in high-fat diet-induced obese rats
  JOURNAL   Endocrinology 152 (4), 1284-1289 (2011)
   PUBMED   21239437
  REMARK    GeneRIF: Data report that DNA hypermethylation is correlated with a
            decline in hepatic glucokinase (Gck) and L-type pyruvate kinase
            (LPK) expression in high-fat diet-induced obese rats as compared
            with the control diet group.
REFERENCE   6  (bases 1 to 2922)
  AUTHORS   Kanno,H., Fujii,H., Hirono,A., Omine,M. and Miwa,S.
  TITLE     Identical point mutations of the R-type pyruvate kinase (PK) cDNA
            found in unrelated PK variants associated with hereditary hemolytic
            anemia
  JOURNAL   Blood 79 (5), 1347-1350 (1992)
   PUBMED   1536957
REFERENCE   7  (bases 1 to 2922)
  AUTHORS   Brand,I.A. and Heinickel,A.
  TITLE     Key enzymes of carbohydrate metabolism as targets of the 11.5-kDa
            Zn(2+)-binding protein (parathymosin)
  JOURNAL   J Biol Chem 266 (31), 20984-20989 (1991)
   PUBMED   1834654
REFERENCE   8  (bases 1 to 2922)
  AUTHORS   Marie,J., Simon,M.P., Lone,Y.C., Cognet,M. and Kahn,A.
  TITLE     Tissue-specific heterogeneity of the 3'-untranslated region of
            L-type pyruvate kinase mRNAs
  JOURNAL   Eur J Biochem 158 (1), 33-41 (1986)
   PUBMED   2874025
REFERENCE   9  (bases 1 to 2922)
  AUTHORS   Lone,Y.C., Simon,M.P., Kahn,A. and Marie,J.
  TITLE     Complete nucleotide and deduced amino acid sequences of rat L-type
            pyruvate kinase
  JOURNAL   FEBS Lett 195 (1-2), 97-100 (1986)
   PUBMED   3080337
REFERENCE   10 (bases 1 to 2922)
  AUTHORS   Inoue,H., Noguchi,T. and Tanaka,T.
  TITLE     Complete amino acid sequence of rat L-type pyruvate kinase deduced
            from the cDNA sequence
  JOURNAL   Eur J Biochem 154 (2), 465-469 (1986)
   PUBMED   3002799
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Apr 23, 2008 this sequence version replaced NM_012624.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M11709.1, SRR26643291.6649.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                JAXUCZ010000002.1  176841264-176841285
            23-205              JAXUCZ010000002.1  176841870-176842052
            206-297             JAXUCZ010000002.1  176844017-176844108
            298-429             JAXUCZ010000002.1  176844250-176844381
            430-616             JAXUCZ010000002.1  176844516-176844702
            617-887             JAXUCZ010000002.1  176845019-176845289
            888-1038            JAXUCZ010000002.1  176845402-176845552
            1039-1191           JAXUCZ010000002.1  176845765-176845917
            1192-1358           JAXUCZ010000002.1  176846011-176846177
            1359-1540           JAXUCZ010000002.1  176847398-176847579
            1541-2922           JAXUCZ010000002.1  176848256-176849637
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..2922
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /note="pyruvate kinase L/R"
                     /db_xref="GeneID:24651"
                     /db_xref="RGD:3336"
     exon            1..22
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..1647
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /EC_number="2.7.1.40"
                     /note="pyruvate kinase isozymes R/L; L-PK; pyruvate
                     kinase, liver and red blood cell; pyruvate kinase isozymes
                     L/R; pyruvate kinase, liver and RBC"
                     /codon_start=1
                     /product="pyruvate kinase PKLR"
                     /protein_id="NP_036756.3"
                     /db_xref="GeneID:24651"
                     /db_xref="RGD:3336"
                     /translation="MEGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCL
                     LDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIAN
                     IREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKF
                     QTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSR
                     KGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQN
                     IKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGK
                     PVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHA
                     IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSA
                     QLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIES
                     GKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS"
     exon            23..205
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            206..297
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            298..429
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            430..616
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            617..887
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1038
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1191
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1358
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1540
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..2922
                     /gene="Pklr"
                     /gene_synonym="PK1; PKL; Pklg"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aagcaacgta gcagcatgga agggccagcg ggataccttc gacgtgcgag tgtggctcaa
       61 ctgacccagg agctgggcac tgccttcttc cagcagcagc aactgcccgc agctatggcg
      121 gacaccttcc tggaacacct ctgccttctg gatatcgact cacagcctgt ggctgctcgt
      181 agcaccagca tcattgccac cattgggcca gcatcccgct ctgtggaccg cctcaaggag
      241 atgatcaaag cagggatgaa cattgcacga ctcaacttct cccatggctc ccatgagtac
      301 catgcagaat ccatcgccaa catccgggag gcaactgaga gttttgcaac ctccccactc
      361 agctacagac ctgtggccat cgccctggac accaagggac ctgagatacg aaccggagtc
      421 ttgcaggggg gtccggagtc ggaggtggaa attgtgaagg gctcacaggt gctggtgacg
      481 gtggacccga agttccagac aaggggcgat gcaaagacag tgtgggtgga ctaccacaat
      541 atcacccggg tcgttgcagt ggggggccgc atctacattg acgacgggct catctcctta
      601 gtggtacaga aaatcggccc agagggactg gtgacagaag tggagcacgg tggtatcttg
      661 ggcagcagga agggtgtgaa cttgccaaac actgaggtgg acctgcccgg gctgtctgag
      721 caagaccttt tggatctgcg cttcggggtg cagcataatg tggacatcat ctttgcctcc
      781 tttgtgcgga aagccagtga cgtgttagca gtccgggatg ccctggggcc agaaggacag
      841 aacatcaaaa ttatcagcaa aatcgagaac catgaaggcg tgaagaagtt tgatgaaatt
      901 ctagaagtga gcgatggcat catggtggca cggggtgacc tgggcattga gatccctgcg
      961 gagaaggttt tcttggctca gaagatgatg attggacgct gcaacctggc cggcaagcct
     1021 gtcgtttgtg ccacacagat gctggagagc atgatcacta aggctcgacc aactcgggcg
     1081 gagacaagcg atgtggccaa tgccgtgctg gatggggctg actgtatcat gctgtccgga
     1141 gagaccgcca agggcagttt tcctgtggaa gctgtaatga tgcaacatgc gattgcgcgg
     1201 gaggcagagg ccgctgtgta ccaccgccag ttgtttgagg agctacgccg ggcagcgccg
     1261 ctgagccgtg acccaactga ggtcactgcg attggagccg tggaggcttc cttcaagtgc
     1321 tgtgcagcag ccatcatcgt gctgacgaag actggccgtt cagcccagct tctatctcaa
     1381 taccgacctc gggcggctgt cattgctgtg actcgatctg cccaggctgc ccgacaggtc
     1441 cacctgtccc gaggagtctt ccccttgctc taccgtgagc ctccagaggc catctgggca
     1501 gatgatgtgg atcgaagggt ccaatttggc attgaaagtg gaaagctccg tggtttcctc
     1561 cgtgtgggtg atctggtgat tgtggtgaca ggttggcggc ctggctctgg ctataccaac
     1621 atcatgcggg tgctgagcgt atcctgaaat gcctctcccc attctgaccc agttacaccc
     1681 tatttctttc aacccacacc cctcccatag tcctacatct gccatctagc cccatccctg
     1741 tgctttacac aggccctgaa tgtctgtgtc caattataca gtggccaccg gcagcatcgg
     1801 ttgtatatcc ctgtctcaat ccgctcagct ggactctaag ataccctgag cctttaatcc
     1861 cagcccagct ggttgattcg attccttccg ggtcccaatc attggaatgg gggagtggaa
     1921 acagggtgat cttgtccaat tttcatacaa tcatgatttt aaaacactgt ctgatataac
     1981 cctcatgatc agtttcctag caaagtgtca tctcctaatg gcctcaagtc agggcagaat
     2041 actccttcaa ggagcacagc tccacacttt agggaaggct ggggcagctg ggtactggag
     2101 agaactaaga caggctggct tttctctctc tctctctctt tttttttttc ttttcttttt
     2161 ctttttttcg gagctgggga ccgaacccag ggccttgtgc ttgctaggca agcgctctac
     2221 cactgagcta aatccccaac cccagctttt ctctttttaa tacaagctct cactggcctc
     2281 aaactcctaa gtcctcctgc ctggccctcc taagggtagg gactacaggc atgagtgacc
     2341 agctggactt cgggtagcct tattttctta ctgactccac aaaccatggt tgttctcctg
     2401 cccactgctc tgctgggtca gatgatccag aaattcttcc acaaccactt ggctcccaca
     2461 tacaaattag aagcaaaact gaatcttttc ttttaaaccc aactgtttag gtgcaattat
     2521 aaaaacaact cccacaggca aagaatccca gaatctccta ccctaggaga tgtatagtcc
     2581 tggccccacc catcaatgct gtagtatact cctgaagcgg gacagaactg gtggacaggg
     2641 gactcctctt gtccctaaga aagtggaggc actgttggcc cacccctcct aggtttgaat
     2701 actccaggcc ctcctcttca gcaccaacag caaatccaga tgaggaaaaa aaaataagtg
     2761 cagttctcct gctgccctcc tcttttcact acctcaatac agcaagtttg agtattgctg
     2821 ctgatggcag tgtgcaagga ccacaaagat gtcccccctc agccccctac cagaaggtgg
     2881 agaggacaga ggaatgaata ataaagtgaa tgcgtcaaat ta
//
