LOCUS       NM_138896               4714 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2),
            transcript variant 1, mRNA.
ACCESSION   NM_138896 XM_346858
VERSION     NM_138896.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Zhong,J., Wang,H., Chen,W., Sun,Z., Chen,J., Xu,Y., Weng,M.,
            Shi,Q., Ma,D. and Miao,C.
  TITLE     Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1
            macrophage polarization by activating JNK and p38 pathways
  JOURNAL   Cell Death Dis 8 (5), e2763 (2017)
   PUBMED   28471450
  REMARK    Erratum:[Cell Death Dis. 2018 Jul 16;9(8):782. doi:
            10.1038/s41419-018-0828-y.. PMID: 30013073]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Lignitto,L., Carlucci,A., Sepe,M., Stefan,E., Cuomo,O., Nistico,R.,
            Scorziello,A., Savoia,C., Garbi,C., Annunziato,L. and Feliciello,A.
  TITLE     Control of PKA stability and signalling by the RING ligase praja2
  JOURNAL   Nat Cell Biol 13 (4), 412-422 (2011)
   PUBMED   21423175
  REMARK    GeneRIF: Data suggest that praja2 regulates the stability and total
            concentration of protein kinase A R subunits, tuning the strength
            and duration of PKA signal output in response to cAMP.
REFERENCE   3  (bases 1 to 4714)
  AUTHORS   Nakayama,M., Miyake,T., Gahara,Y., Ohara,O. and Kitamura,T.
  TITLE     A novel RING-H2 motif protein downregulated by axotomy: its
            characteristic localization at the postsynaptic density of
            axosomatic synapse
  JOURNAL   J Neurosci 15 (7 Pt 2), 5238-5248 (1995)
   PUBMED   7623148
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB577390.1, BC074015.1 and JAXUCZ010000009.1.
            
            On Mar 28, 2013 this sequence version replaced NM_138896.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC074015.1, SRR26360181.1081394.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                CB577390.1         1-75
            76-2732             BC074015.1         1-2657
            2733-4714           JAXUCZ010000009.1  111403624-111405605 c
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q37"
     gene            1..4714
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /note="praja ring finger ubiquitin ligase 2"
                     /db_xref="GeneID:192256"
                     /db_xref="RGD:620273"
     exon            1..117
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            118..235
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..2328
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase Praja-2; protein carrying the
                     RING-H2 sequence motif; praja 2, RING-H2 motif containing;
                     praja ring finger 2, E3 ubiquitin protein ligase;
                     RING-type E3 ubiquitin transferase Praja-2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Praja-2 isoform 1"
                     /protein_id="NP_620251.1"
                     /db_xref="GeneID:192256"
                     /db_xref="RGD:620273"
                     /translation="MSQYTEKEPSVMDQDSSKAAWPRAAGGYQTITGRRYGRRHAYVS
                     FKPCMTRHERSLGRAGDDYEVLELDDVAKENTAGSSSLDQVHPSLPSETTVEKSETEI
                     PTCGPALNQSTESNPSVATVCHSEEVRETLDSSTNLQNHAERECTPAVCNASSVQNGI
                     VLVHTDSYDPDSKHDENDSLQLCAQAVEGGRRQKVLGNAVFELENGEVERYADLCPSV
                     PSLSGEIREESEELGSALLEKNSAGDAEAVHQDGQEFQRSSEDGIVRKRRQDDTDQGR
                     QTENSTEDADCVPGHVEQNTSERANHHGSSPEQVVRPKVRKVISSSQVDQESGFNRHE
                     AKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPSYSRVTPREAERHRATA
                     ENGATASGRQEARENAFWNACGEYYQLFDKDEDSSECSDGEWSASLPHRFSGTEKDQS
                     SSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNE
                     SSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAIS
                     YVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDH
                     TAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVI
                     DASAAASSEPDLDASPANDNAEEAP"
     exon            236..436
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            437..1481
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1667
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1853
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1965
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2080
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2202
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..4714
                     /gene="Pja2"
                     /gene_synonym="Neurodap1; praja2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgccgctg cagccgctgc gcgttcggcg ggggaggagt cggaggcgga gaagaaggcg
       61 gtggtggcgg gtggaggatc gagcgctgtt ctcgctctgg agccgctgca catctcggaa
      121 tcatttgcag cttattcact gtgtaaggaa aaaccaaatt acatccataa ttctattgaa
      181 ctggaagcat agacgctgcc atatatgtca cagtatacag aaaaagaacc atcagtaatg
      241 gatcaagact ccagcaaggc tgcgtggcct agagcagcag gaggatacca gacgattaca
      301 ggcaggaggt acggaaggag acatgcgtat gtcagtttta agccatgtat gaccaggcat
      361 gaacggagct tgggtcgggc tggcgatgac tatgaagttc tggaactgga tgacgttgcc
      421 aaggaaaaca ccgcaggttc cagttcattg gatcaagtcc atccttcttt acccagtgaa
      481 actacagttg aaaaaagtga aacagaaatt cctacttgtg gtccagcact gaatcaaagc
      541 acggagagca acccatccgt tgccacagtg tgtcatagtg aggaagtcag ggagacctta
      601 gacagcagta cgaatcttca gaatcacgct gagagagagt gtacgccagc agtttgtaat
      661 gcctcaagtg tccagaatgg aattgtgttg gttcatactg actcttatga tccagacagc
      721 aagcatgatg agaatgactc tcttcaactt tgtgcccaag ctgtggaagg tggtagacgt
      781 cagaaggtat taggcaatgc agtctttgag ctggaaaatg gagaggtaga gagatatgct
      841 gatctgtgtc cctcagttcc ctctctcagt ggtgaaataa gggaggagtc tgaagagcta
      901 ggttcagcac tcttagagaa aaattctgct ggcgatgcgg aggctgtcca tcaggatggg
      961 caggaatttc agaggtcttc tgaagacggc attgttagaa agaggcgaca agatgatacc
     1021 gatcagggaa gacagacaga aaattcaact gaagatgcag actgtgttcc aggccatgtt
     1081 gaacaaaata ctagtgagag agccaatcac catggaagtt ctcctgaaca ggtagtgagg
     1141 cccaaagtta gaaaagtgat cagttcaagc caggtggacc aagagagcgg ttttaatagg
     1201 cacgaggcta agcaaagaag tgttcagagg tggagagagg ctctggaagt tgaggaatgc
     1261 agttcagatg accctataat caagtgtgac gattatgatg gagaccatga ctgcatgttc
     1321 ctaaccccat cctactcaag agttacgcca agggaagcag aacgtcaccg tgcgacagca
     1381 gaaaatggag ccacagcttc aggaaggcaa gaggctcggg aaaatgcctt ttggaatgcc
     1441 tgtggagagt attaccagct ctttgacaaa gacgaagaca gttcagagtg cagtgatggg
     1501 gaatggtctg cttctctgcc tcaccgattt tctggcacag aaaaagacca gtcctcaagc
     1561 gatgaaagct gggaaactct gccaggaaaa gatgagaatg aacctgagct acagagtgat
     1621 agtagtggcc ctgaggaaga aaaccaagaa ttgtctcttc aggagggaga acagacgtcc
     1681 ttggaggagg gggagattcc ctggttacag tacaatgagg tcaatgagag cagcagcgat
     1741 gaagggaacg agcctgccaa tgagtttgca caaccagaag ctttcatgtt ggatgggaac
     1801 aacaacctgg aggacgactc gagcgtgagt gaagacctgg atgtggactg gagcctattt
     1861 gatggttttg cggatggact tggtgttgct gaagctattt cctacgtgga tcctcagttc
     1921 ctcacctaca tggcactaga agaacgctta gcccaggcta tggagactgc actggcacac
     1981 ttagagtctc ttgctgtaga cgttgaggtg gctaacccac ctgccagtaa ggaaagcatc
     2041 gacggacttc cagaaaccct tgtcctagaa gatcacaccg ctattggtca ggagcagtgc
     2101 tgtcccatct gctgcagtga gtacattaag gatgacatcg caacagagct gccctgccat
     2161 cacttctttc ataagccttg tgtctccatt tggctacaga agtccggaac gtgccctgtg
     2221 tgccgccgcc acttcccacc tgcagtgatt gacgcatctg cagctgcttc ctctgaacca
     2281 gaccttgatg cctcgcctgc aaacgacaat gctgaggaag ccccgtaagc cgtcacgggg
     2341 aaatgagatc gaaattctat cacagtaaat ctgcaaattc cttctaaatc tgatgtgcaa
     2401 ataattatat ataaatatat ttaaaatgct ctatatagta tatgccgtag tttagaaaga
     2461 gaatattaac ctttctaaac tgaatttagg tttgcagaag atactaaaca ttttcaagct
     2521 gaatgttgaa gcagtgcatc cattttcttt agttgaacat ggtgtccatt gtaacgtcag
     2581 tagtcatctc tgtggcatac gtttctgttt tgtctgaagt gctgccacta agagatcaga
     2641 attaagctct cctttccaca tcagatgtga aaactgtgat cacaacagta gtacagtttg
     2701 gtttcattga aaataaactg aattctaaag catgcttttt cactggtccc tttgcttttg
     2761 ctacttcgag acctcttggt ttatataaca ctgaggttaa gattaaagct tttcagaatg
     2821 ccaggcaaag actagagttt tgacccgaac acacacacaa aataaaaaaa taaaaaataa
     2881 attacatctt agatgtaaac ttcctgaaac tgtcatgtaa taaactattc agtgaacgtt
     2941 ggatgtgttt aaaggctagt aaagcatgtg aattatctga tgtgcttcat gctcggcaca
     3001 gtctcatgag cttggcctat gaaacactgt ctaagccctc atgcagtctg cttctacttg
     3061 ttaaacactg aaggtccaga ccagtcttgg catctgatta agaaattgga tgtagttagc
     3121 taaggtagtt tgggttctac tttaggagat ctgaagattt gcacatggcc atgtctgtta
     3181 cagcatcacc ccagattaga aaggcacagc ttttaggtgg tcacacgagt aggcaagtct
     3241 gtgcagacgc tggtgtgata ggtagcttac aggtgagccc tgacatcagc acagggacaa
     3301 cggctcagca ggcccagcac tggagcccat ccgcgtgcac atctacctgc cctgctcatc
     3361 cagttcggga atatttctat agcttcccaa atcagcttca tctgatttgt gtaatatatc
     3421 tgagtattta tagtctttct tttttctcca gtcttaaaaa taaatgaatt ttcactgttg
     3481 gcacatttga ggcttaaata taagaaacct aacacttgca ttctgatttt tgcatatatt
     3541 gtaaatgtgg ctggtattta cagcgaatcc tgtgtgtcct tttatgggta aaacaaaagt
     3601 gaacattgca tgcaagtaat gtggtgaatt tgtaatttag gggttttctg gggcttctgt
     3661 gactgtggat gcttaaattc aggccttaat gttgctctag ctagcatgtt tccttctaat
     3721 gtacatagtc agcctgtatc cactaatctc tgcttgtctt ctcctccgtg gtcttcacac
     3781 cgtatccact gatggtcagt tagtgtcaga ggtcacccag gttagcatca gttcccatgt
     3841 gtgccgtgcg gattgtgagc cgtgtggttg cttggttggt tttcttttat gtactttgat
     3901 tagctgtggc acttactgag taaacttacg ttgctgcaga ctgctgtgta acaagccacg
     3961 ttttgtgttt agtcagtatt acacacggga ccttgtttta ttcttctaga tagcagctgt
     4021 cccaaagaaa atatttcttc tttgcctatt aagatttagc tattatctgc cagttgttaa
     4081 ggggtttgat tccaaactca accagagttg aacgtaagaa agctgctgta ctcttgctgt
     4141 ccatctgtta cgtcctccgt tgttggctcc tgactacgta cccacggctg ctgaaggaga
     4201 cttgttttaa tttgttttaa aaaggaaaat gaagttttag gactttgatg gtggggaagc
     4261 tgacaagttc agaccccagt ctgtcatttg ctcttagctt ggatcctttt gaaaagttaa
     4321 gaatatatga aggtaaatta gaaaaagtaa aaatattgat aaaatgtcat ttatcttatg
     4381 tctattttat gttgttattt ggcctattaa ttttaatttt ttgaacattt tcttatttct
     4441 tgtaatatga atgccgatat ttaaagtttg actcacttgg gtttcttttg tgtttcttag
     4501 ttgttatctc taagctaact aacttttaag attatttgtg atatggattt atataagctg
     4561 ttaaatatat atgagctgtt aaaatggaat gcaaggtttt ccaaagacca ggtctaactg
     4621 taatgattgg tttattgttc tacaatccca gcccggcatt ttccgtgtaa atcataaaca
     4681 ataaacagga tatactcagt gttcatttct aata
//
