LOCUS       NM_001271133            3109 bp    mRNA    linear   ROD 14-MAR-2025
DEFINITION  Rattus norvegicus piwi-like RNA-mediated gene silencing 4 (Piwil4),
            transcript variant 2, mRNA.
ACCESSION   NM_001271133 XM_001072752
VERSION     NM_001271133.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Henaoui,I.S., Jacovetti,C., Guerra Mollet,I., Guay,C., Sobel,J.,
            Eliasson,L. and Regazzi,R.
  TITLE     PIWI-interacting RNAs as novel regulators of pancreatic beta cell
            function
  JOURNAL   Diabetologia 60 (10), 1977-1986 (2017)
   PUBMED   28711973
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Sivagurunathan,S., Palanisamy,K., Arunachalam,J.P. and
            Chidambaram,S.
  TITLE     Possible role of HIWI2 in modulating tight junction proteins in
            retinal pigment epithelial cells through Akt signaling pathway
  JOURNAL   Mol Cell Biochem 427 (1-2), 145-156 (2017)
   PUBMED   28025795
REFERENCE   3  (bases 1 to 3109)
  AUTHORS   Yang,Z., Chen,K.M., Pandey,R.R., Homolka,D., Reuter,M.,
            Janeiro,B.K., Sachidanandam,R., Fauvarque,M.O., McCarthy,A.A. and
            Pillai,R.S.
  TITLE     PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from
            Cytosolic Targets of the Mouse piRNA Pathway
  JOURNAL   Mol Cell 61 (1), 138-152 (2016)
   PUBMED   26669262
REFERENCE   4  (bases 1 to 3109)
  AUTHORS   Pastor,W.A., Stroud,H., Nee,K., Liu,W., Pezic,D., Manakov,S.,
            Lee,S.A., Moissiard,G., Zamudio,N., Bourc'his,D., Aravin,A.A.,
            Clark,A.T. and Jacobsen,S.E.
  TITLE     MORC1 represses transposable elements in the mouse male germline
  JOURNAL   Nat Commun 5, 5795 (2014)
   PUBMED   25503965
  REMARK    Erratum:[Nat Commun. 2015 Jul 10;6:7604. doi: 10.1038/ncomms8604.
            PMID: 26159654]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3109)
  AUTHORS   Keam,S.P., Young,P.E., McCorkindale,A.L., Dang,T.H., Clancy,J.L.,
            Humphreys,D.T., Preiss,T., Hutvagner,G., Martin,D.I., Cropley,J.E.
            and Suter,C.M.
  TITLE     The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in
            somatic cells
  JOURNAL   Nucleic Acids Res 42 (14), 8984-8995 (2014)
   PUBMED   25038252
REFERENCE   6  (bases 1 to 3109)
  AUTHORS   Shoji,M., Tanaka,T., Hosokawa,M., Reuter,M., Stark,A., Kato,Y.,
            Kondoh,G., Okawa,K., Chujo,T., Suzuki,T., Hata,K., Martin,S.L.,
            Noce,T., Kuramochi-Miyagawa,S., Nakano,T., Sasaki,H., Pillai,R.S.,
            Nakatsuji,N. and Chuma,S.
  TITLE     The TDRD9-MIWI2 complex is essential for piRNA-mediated
            retrotransposon silencing in the mouse male germline
  JOURNAL   Dev Cell 17 (6), 775-787 (2009)
   PUBMED   20059948
REFERENCE   7  (bases 1 to 3109)
  AUTHORS   Aravin,A.A., van der Heijden,G.W., Castaneda,J., Vagin,V.V.,
            Hannon,G.J. and Bortvin,A.
  TITLE     Cytoplasmic compartmentalization of the fetal piRNA pathway in mice
  JOURNAL   PLoS Genet 5 (12), e1000764 (2009)
   PUBMED   20011505
REFERENCE   8  (bases 1 to 3109)
  AUTHORS   Aravin,A.A., Sachidanandam,R., Bourc'his,D., Schaefer,C., Pezic,D.,
            Toth,K.F., Bestor,T. and Hannon,G.J.
  TITLE     A piRNA pathway primed by individual transposons is linked to de
            novo DNA methylation in mice
  JOURNAL   Mol Cell 31 (6), 785-799 (2008)
   PUBMED   18922463
REFERENCE   9  (bases 1 to 3109)
  AUTHORS   Kuramochi-Miyagawa,S., Watanabe,T., Gotoh,K., Totoki,Y., Toyoda,A.,
            Ikawa,M., Asada,N., Kojima,K., Yamaguchi,Y., Ijiri,T.W., Hata,K.,
            Li,E., Matsuda,Y., Kimura,T., Okabe,M., Sakaki,Y., Sasaki,H. and
            Nakano,T.
  TITLE     DNA methylation of retrotransposon genes is regulated by Piwi
            family members MILI and MIWI2 in murine fetal testes
  JOURNAL   Genes Dev 22 (7), 908-917 (2008)
   PUBMED   18381894
REFERENCE   10 (bases 1 to 3109)
  AUTHORS   Carmell,M.A., Girard,A., van de Kant,H.J., Bourc'his,D.,
            Bestor,T.H., de Rooij,D.G. and Hannon,G.J.
  TITLE     MIWI2 is essential for spermatogenesis and repression of
            transposons in the mouse male germline
  JOURNAL   Dev Cell 12 (4), 503-514 (2007)
   PUBMED   17395546
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Sep 1, 2012 this sequence version replaced XM_001072752.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMEA5760383, SAMEA5760434
                              [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               JAXUCZ010000008.1  19861118-19861220   c
            104-235             JAXUCZ010000008.1  19857313-19857444   c
            236-450             JAXUCZ010000008.1  19854407-19854621   c
            451-572             JAXUCZ010000008.1  19849931-19850052   c
            573-653             JAXUCZ010000008.1  19848140-19848220   c
            654-851             JAXUCZ010000008.1  19844447-19844644   c
            852-963             JAXUCZ010000008.1  19842929-19843040   c
            964-1087            JAXUCZ010000008.1  19835733-19835856   c
            1088-1205           JAXUCZ010000008.1  19833845-19833962   c
            1206-1317           JAXUCZ010000008.1  19831229-19831340   c
            1318-1502           JAXUCZ010000008.1  19829442-19829626   c
            1503-1575           JAXUCZ010000008.1  19826182-19826254   c
            1576-1776           JAXUCZ010000008.1  19824687-19824887   c
            1777-1880           JAXUCZ010000008.1  19823626-19823729   c
            1881-1951           JAXUCZ010000008.1  19823388-19823458   c
            1952-2105           JAXUCZ010000008.1  19822130-19822283   c
            2106-2234           JAXUCZ010000008.1  19819948-19820076   c
            2235-2382           JAXUCZ010000008.1  19819279-19819426   c
            2383-3109           JAXUCZ010000008.1  19817986-19818712   c
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q12"
     gene            1..3109
                     /gene="Piwil4"
                     /note="piwi-like RNA-mediated gene silencing 4"
                     /db_xref="GeneID:689972"
                     /db_xref="RGD:1311015"
     CDS             1..2499
                     /gene="Piwil4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     piwi-like protein 4; piwi-like 4"
                     /codon_start=1
                     /product="piwi-like protein 4 isoform 2"
                     /protein_id="NP_001258062.1"
                     /db_xref="GeneID:689972"
                     /db_xref="RGD:1311015"
                     /translation="MSGRARVRARGITTGHSAREVERAPRDLMVSASVYELGVSSGDG
                     RRTLVEKRGNGRQDFVDLGVCTREKLTHVKDSKTGSSGIPVQLATNLFHLDLPQDWQL
                     YQYHVTYSPDLASRRLRTALLYNHSILSDKAKAFDGTSLFLSEKLDHKVTELTSETQR
                     GETVKITLTLTSELFPNSPMCIQFFNVIFRKIFKKLSMYQIGRNYYKPSEPVEIPQYK
                     LSLWPGFAISVSRFESKLLFNADVNYKVLRNETVLEFMTDLCLRTDMSCFTETCHKQL
                     VGLIVLTRYNNKTYRIDDIDWSVKPTHAFQKRDGSETTYVDYYKQQYDITLSDLNQPV
                     LVSLLKRRRNDNSEPQMVHLIPELCFLTGLTSQATSDFRLMKAVAEETRLSPMGRQQQ
                     LARLADDIQRNQVTRFELETWGLHFGSQLSLTGRVVPSEKILLQDHTCQPAFAADWSK
                     DMRSCKVLSSEPLNTWLIVCCSRAEHLIESFLSCLRRVGGSTGFSVGHPKIIKVDENP
                     AAFLRAIQLHVNPDVQLVMCILPSNQKNYYDSIKRYLSSDCPVPSQCVLTRTLNKQGM
                     MLSVATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDICRDALSKDVAVVGFVASINSR
                     ITRWFSRCVLQRTAADIADCLKVCMTGALNKWYKHNHDLPARIIAYRDGVGNGQLKAV
                     LEYEVPQLLSSVTECGSDARSCRLSVVVVRKRCLLRLFAESGHTLQNPPLGTVVDSEA
                     TRPEWYDFYLTSQTANRGTVSPTYYNVIYDDNALKPDHMQRLTFKLCHLYYNWQGVIS
                     VPAPCQYAHKLTFLVAQSIHKEPSLELANKLFYL"
     exon            1..103
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            104..235
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            236..450
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            451..572
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            573..653
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            654..851
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            852..963
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1087
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1205
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1317
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1502
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1575
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1776
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1880
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1951
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2105
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2234
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2382
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..3109
                     /gene="Piwil4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgagtggac gggcccgtgt gagggcgcga ggcatcacca ctggccacag tgccagggaa
       61 gtggagcggg ccccaagaga cctcatggtg agtgcttctg tctatgagct tggcgtctct
      121 tctggtgatg gtagacgtac tctcgtggaa aaaagaggca atggcagaca ggacttcgtc
      181 gatttgggtg tctgtaccag ggagaagttg actcacgtaa aagattctaa aacaggttct
      241 agcggaatac ctgtgcagct ggctacaaat ctcttccatt tagatttgcc ccaggattgg
      301 cagctatacc agtaccatgt cacatacagc ccggatttag cctctaggag gctgaggact
      361 gctctgctgt ataaccacag catactctcc gacaaggcaa aggcattcga cggcaccagc
      421 cttttcctct cagaaaagct ggaccacaaa gtcacagaac tgacaagtga aacccaaaga
      481 ggggagactg tgaagataac tctcaccctg accagtgagc tgttcccaaa ctccccgatg
      541 tgcattcagt ttttcaatgt catcttcaga aagatcttta aaaagttgag catgtaccag
      601 attggacgga actactataa gccttcagag ccagtggaga tcccccagta caaattgtcc
      661 ctgtggcctg ggtttgccat ttctgtgtct cgctttgaaa gcaaactcct gttcaatgct
      721 gacgtaaact ataaagtcct ccgcaatgag actgttctgg aattcatgac tgatctctgc
      781 ctcagaaccg acatgtcatg cttcactgag acgtgccaca aacagctagt ggggctcatt
      841 gtccttacca ggtacaacaa caaaacatat cgtatcgatg acattgactg gtctgtgaag
      901 cccacacatg cctttcagaa gcgggatggc tcagagacca cgtatgtgga ttactacaag
      961 cagcaatatg atatcacctt atctgaccta aatcagccag tgcttgtcag tctgttgaaa
     1021 agaaggagaa atgataactc cgagcctcag atggtccacc tgatcccgga gctctgcttt
     1081 ctcacgggcc tgaccagcca agcaacctca gacttccgct tgatgaaggc agtcgctgaa
     1141 gaaactcggc tcagtcctat gggaaggcag cagcaactag cccgactggc agatgacatc
     1201 cagagaaacc aagtgactcg gtttgagctt gagacctggg gattgcattt tggaagccag
     1261 ctatccctga ccggccgggt tgttccctct gaaaagatcc tgctgcagga ccacacatgt
     1321 caacctgcat ttgctgctga ttggtccaaa gatatgcgat cttgcaaggt tctgagttct
     1381 gagcctttga atacatggtt gatcgtgtgc tgtagcaggg ctgagcattt gattgaatcc
     1441 ttcctgagct gtctgaggag agttggaggt tccacgggat tcagtgtggg ccacccaaaa
     1501 atcataaaag tggatgaaaa ccctgcagcg ttccttcgag ccatccagct gcacgtcaac
     1561 cccgatgttc agttggtgat gtgcatcctg ccttctaatc agaagaacta ttacgactcc
     1621 attaaaagat atttgagctc cgactgcccg gtcccaagcc agtgtgtgct gacccggacc
     1681 ttgaacaaac agggaatgat gctgagtgtg gccaccaaga tcgccatgca aatgacctgc
     1741 aaactcgggg gcgagctgtg ggctgtggag atcccattga agtcactgat ggtcgtgggc
     1801 atcgacatct gtagagacgc cctcagcaag gatgtagcgg tcgtcgggtt tgtcgccagc
     1861 attaactcca ggatcaccag gtggttttcc cgctgtgtcc tccagagaac ggcagctgat
     1921 attgcagatt gcctgaaagt ctgcatgact ggtgctctca acaagtggta caaacacaac
     1981 cacgatttgc cggcacggat aatcgcgtac cgggatggtg tgggcaacgg ccagctaaag
     2041 gcagttttgg aatacgaagt cccacagctg ctgagcagtg tgacagaatg tggctccgac
     2101 gccaggagct gtagactgtc agtggtcgtg gtcaggaaga ggtgtctgct gcgtctcttt
     2161 gccgagtctg gccatacgct acagaacccc ccacttggca ctgtcgtgga ctcagaagca
     2221 acacgtccag agtggtatga cttctacctg accagccaga ctgctaaccg ggggactgtt
     2281 agtcccacct actacaatgt catctatgac gacaacgctc tgaagcctga ccatatgcag
     2341 cggctgacct tcaaactgtg ccacctctac tacaactggc agggcgtaat cagtgtccct
     2401 gctccgtgcc agtatgcaca caagctgacc ttcctggtgg cacaaagcat ccacaaggaa
     2461 ccaagtctgg aattagccaa taagctcttc tacctgtgac aagtgggcca atagcatggc
     2521 tcgatggaga cgccaaaaag cggttgctat gctttgttca aatctgccta atgctcaagg
     2581 ctcctgctgc ttaaaggagc ttcagcctga ttttaagtat tgggaaggag agggtttttt
     2641 ggtttttgtt tttttgtttt gttttgtttt gttttttgtt ttctgggttt tttttttgtt
     2701 gttgttgttg ttgtttttaa agtcatctga atcagttgag gacaaaccta tggggatttt
     2761 tgtctcccct gtgtctaaaa acaacaatga caaacaaaca aaaacccaca gagtgatggg
     2821 ttttcttgtg gctctgtcag gcgacatttt actttgtcct ggtttatcct cgtatttttt
     2881 tttttttaag taaatgcttg aggtgaattc tcacatggta tatacgtgat gatattggga
     2941 tatatcatca cagctaactg gattctaaaa caatattcgt gtggtattgt gatggtgttt
     3001 aagaggggtt taacttcttt cctgacatta ccagggttgt tctaagtatc gtagagcatt
     3061 ttgtagagtg ggttaagtaa atactgaaaa taaagactcc ctgagcatc
//
