LOCUS       NM_001107276            4899 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus piwi-like RNA-mediated gene silencing 2 (Piwil2),
            mRNA.
ACCESSION   NM_001107276 XM_001069687 XM_224334
VERSION     NM_001107276.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Lu Y, Zheng X, Hu W, Bian S, Zhang Z, Tao D, Liu Y and Ma Y.
  TITLE     Cancer/testis antigen PIWIL2 suppresses circadian rhythms by
            regulating the stability and activity of BMAL1 and CLOCK
  JOURNAL   Oncotarget 8 (33), 54913-54924 (2017)
   PUBMED   28903391
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Wenda JM, Homolka D, Yang Z, Spinelli P, Sachidanandam R, Pandey RR
            and Pillai RS.
  TITLE     Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI
            Slicing-Triggered Mammalian piRNA Biogenesis and Function
  JOURNAL   Dev Cell 41 (6), 623-637 (2017)
   PUBMED   28633017
REFERENCE   3  (bases 1 to 4899)
  AUTHORS   Sivagurunathan S, Palanisamy K, Arunachalam JP and Chidambaram S.
  TITLE     Possible role of HIWI2 in modulating tight junction proteins in
            retinal pigment epithelial cells through Akt signaling pathway
  JOURNAL   Mol Cell Biochem 427 (1-2), 145-156 (2017)
   PUBMED   28025795
REFERENCE   4  (bases 1 to 4899)
  AUTHORS   Barau J, Teissandier A, Zamudio N, Roy S, Nalesso V, Herault Y,
            Guillou F and Bourc'his D.
  TITLE     The DNA methyltransferase DNMT3C protects male germ cells from
            transposon activity
  JOURNAL   Science 354 (6314), 909-912 (2016)
   PUBMED   27856912
REFERENCE   5  (bases 1 to 4899)
  AUTHORS   Yang Z, Chen KM, Pandey RR, Homolka D, Reuter M, Janeiro BK,
            Sachidanandam R, Fauvarque MO, McCarthy AA and Pillai RS.
  TITLE     PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from
            Cytosolic Targets of the Mouse piRNA Pathway
  JOURNAL   Mol Cell 61 (1), 138-152 (2016)
   PUBMED   26669262
REFERENCE   6  (bases 1 to 4899)
  AUTHORS   Watanabe T, Totoki Y, Toyoda A, Kaneda M, Kuramochi-Miyagawa S,
            Obata Y, Chiba H, Kohara Y, Kono T, Nakano T, Surani MA, Sakaki Y
            and Sasaki H.
  TITLE     Endogenous siRNAs from naturally formed dsRNAs regulate transcripts
            in mouse oocytes
  JOURNAL   Nature 453 (7194), 539-543 (2008)
   PUBMED   18404146
REFERENCE   7  (bases 1 to 4899)
  AUTHORS   Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A,
            Ikawa M, Asada N, Kojima K, Yamaguchi Y, Ijiri TW, Hata K, Li E,
            Matsuda Y, Kimura T, Okabe M, Sakaki Y, Sasaki H and Nakano T.
  TITLE     DNA methylation of retrotransposon genes is regulated by Piwi
            family members MILI and MIWI2 in murine fetal testes
  JOURNAL   Genes Dev 22 (7), 908-917 (2008)
   PUBMED   18381894
REFERENCE   8  (bases 1 to 4899)
  AUTHORS   Aravin AA, Sachidanandam R, Girard A, Fejes-Toth K and Hannon GJ.
  TITLE     Developmentally regulated piRNA clusters implicate MILI in
            transposon control
  JOURNAL   Science 316 (5825), 744-747 (2007)
   PUBMED   17446352
REFERENCE   9  (bases 1 to 4899)
  AUTHORS   Aravin A, Gaidatzis D, Pfeffer S, Lagos-Quintana M, Landgraf P,
            Iovino N, Morris P, Brownstein MJ, Kuramochi-Miyagawa S, Nakano T,
            Chien M, Russo JJ, Ju J, Sheridan R, Sander C, Zavolan M and Tuschl
            T.
  TITLE     A novel class of small RNAs bind to MILI protein in mouse testes
  JOURNAL   Nature 442 (7099), 203-207 (2006)
   PUBMED   16751777
REFERENCE   10 (bases 1 to 4899)
  AUTHORS   Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita
            Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y and Nakano T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473951.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_224334.4, XM_001069687.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132266, SAMD00132270
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p11"
     gene            1..4899
                     /gene="Piwil2"
                     /note="piwi-like RNA-mediated gene silencing 2"
                     /db_xref="GeneID:306011"
                     /db_xref="RGD:1306964"
     exon            1..138
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            139..375
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..3099
                     /gene="Piwil2"
                     /note="piwi like homolog 2; piwi-like 2"
                     /codon_start=1
                     /product="piwi-like protein 2"
                     /protein_id="NP_001100746.1"
                     /db_xref="GeneID:306011"
                     /db_xref="RGD:1306964"
                     /translation="MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAG
                     RGLVFRKPEESSPPLQPVQKESVGLVSMFRGMGLDTALRAPSKREVPPLGRGVLGRGL
                     SANVVRKDREESRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSDVSLLPLGRAAGS
                     IGRGVDKPPSAFGLTARDPPRLPQPPTLSPTSLHSSDPPPVLTIERKEKELLVKQGSK
                     GTPQSLGLNLIKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQSVTGNVTAFDGSI
                     LYLPVKLQQLVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDM
                     KLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVM
                     HAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITF
                     LEYYSKNYGITVKEDDQPLLIHRPSERQNNHGTLLKGEILLLPELSFMTGIPEKMKKD
                     FRAMKDLTQQINLSPKQHHSALECLLQRISQNETANNELTRWGLSLQKDVHKIEGRLL
                     PMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLE
                     KIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAI
                     KKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVI
                     GMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYY
                     EVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKIST
                     NLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLS
                     PDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCENLFFL"
     exon            376..463
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            464..602
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            603..809
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..920
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1038
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1163
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1244
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1358
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1547
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1632
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1722
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1863
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1977
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2166
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2268
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2385
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2474
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..2580
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2834
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2942
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2943..4899
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgtgttgg aggaatgcgg gtcctggaat aggagggaaa ggaggtggct cgaggagaga
       61 gcgagcgagc actcgcttcg gggctcagtg gcacaagacc taaaaagaaa tctaggcaag
      121 actccagttg gtggaaaggt aacgaattgg aacagaacca acagtcattt ctactgcctc
      181 tcaatggatc ctgtccggcc attgttcagg gggcctactc cagtccaccc atctcaatgt
      241 gttcggatgc caggctgttg gccgcaagct cctagacctt tggaaccagc ttggggtagg
      301 gcaggacctg caggcagagg ccttgtgttt agaaagccag aggaatccag cccaccactc
      361 cagccagtac aaaaggagtc tgtgggtttg gtgtccatgt ttcgtggcat gggtcttgac
      421 acagcattgc gggccccttc aaaacgagaa gtgcctcctt taggcagagg agttctaggt
      481 cgaggcttgt ctgctaacgt agtccgcaag gacagagaag aatcccgttc ctctttgcct
      541 gatccttcgg tgctggcagc tggggacagc aagctggcag aggcttctgt tggatggagt
      601 agaatgctgg gaagaggtag ttctgatgtc tctttgttac cactgggaag agcagctggt
      661 agtataggca gaggagtgga caaacctccc agtgcctttg gccttactgc tcgggatccc
      721 cctcggctgc cccagcctcc aactctgtct ccaacttcac tgcactcctc tgatcctcct
      781 ccagtcctga ctatagagcg aaaggaaaaa gagcttttgg tcaagcaagg atcaaaagga
      841 acacctcagt ctttaggact gaacctcatc aaaatccagt gtcataacga agcagtttat
      901 caatatcatg taactttcag ccccaatgtg gagtgcaaaa gcatgaggtt tggcatgttg
      961 aaggaccacc aatctgtcac tggaaacgtt actgcttttg atggctctat tctttatctt
     1021 cctgttaagc ttcaacaact tgttgagtta aaaagccaga gaaaaactga tgatgccgag
     1081 atcagcatca agattcagct gacaaagatc ctggagccat gttccgactt gtgcattccc
     1141 ttctacaatg ttgtcttccg gcgggtaatg aaacttttgg atatgaagct tgtggggaga
     1201 aacttctatg atcctacaag tgccatggta ctgcagcaac acagattgca gatctggcct
     1261 ggctatgcag ctagtatccg gaggacagat gggggtctct tcttgctcgc tgatgtctct
     1321 cataaggtca ttcggaatga ctctgtactg gatgtcatgc atgctatcta ccagcagaac
     1381 aaggagcact tccaggacga gtgcagcaag cttctggtgg gcagcattgt catcacacgc
     1441 tacaacaacc gtacctaccg cattgacgat gtggactgga acaagacccc taaagacagc
     1501 tttgtaatgt cggatgggaa ggagatcaca ttcctggagt actacagtaa aaactatggg
     1561 atcacagtta aagaagatga ccagccgctg ctcatccacc ggcccagtga gagacagaat
     1621 aaccatggca cgttgctgaa gggtgagatc ctgctgctgc ctgagctttc cttcatgacg
     1681 gggattcctg agaagatgaa gaaggacttc agggccatga aggacttgac tcagcagatt
     1741 aacctgagcc ccaagcagca ccacagtgct ttggaatgcc tgctgcagag aatttcacaa
     1801 aatgagacag ccaacaacga gctgacccgc tgggggctca gtctgcaaaa agatgtccac
     1861 aagatcgaag gccgtcttct accaatggag aggattaact taaggaatac ttcatttgtc
     1921 acatcggagg acctgaactg ggttaaggag gtgaccagag atgcttccat tctgactatt
     1981 cccatgcatt tctgggcact cttttatcca aagagagcaa tggaccaagc cagagaactg
     2041 gttaacatgt tggaaaagat tgctggcccc attggcatgc gcataagccc cccagcctgg
     2101 gttgaactga aggatgaccg aatagagacc tatatcagga ccattcagtc cttactggga
     2161 gttgagggga agatacaaat ggtcgtttgc atcatcatgg gcacacggga tgatctctat
     2221 ggggccatca agaaactgtg ctgtgtgcag tccccagtgc cctcacaggt catcaatgtc
     2281 cgaaccattg gtcaacccac caggcttcgg agtgtggctc agaaaatttt acttcagatg
     2341 aactgtaaac tgggtggtga gctctgggga gtagatattc cactgaaaca attaatggtg
     2401 attgggatgg atgtgtacca cgaccccagc agaggcatgc gctctgtggt tggctttgtt
     2461 gccagcataa atctcacact caccaaatgg tactcgaggg tggtgttcca gatgccgcat
     2521 caggagattg tggacagcct gaagctctgc ctggtgggct ccttgaaaaa gtattatgag
     2581 gtgaaccact gtctcccaga gaagattgtg gtgtaccgag atggagtgtc tgatggccag
     2641 ctaaagacag ttgccaacta tgagatccct cagctgcaga agtgctttga agcctttgat
     2701 aactaccatc ccaagatggt ggtatttgta gttcagaaga aaatcagcac caatctgtac
     2761 cttgctgctc ctgaccactt tgtgaccccc tcccctggga ctgtggttga tcataccata
     2821 accagctgtg agtgggtgga tttctacctt cttgctcatc atgtgcgaca gggctgtggc
     2881 atacctacac actacatctg tgttctgaac actgcaaatc tgagccctga tcacatgcag
     2941 aggctgactt tcaaactatg ccacatgtac tggaattggc ctggtaccat ccgagttcca
     3001 gctccttgca agtatgccca caagctagct ttcctgtccg gacagatttt gcatcatgaa
     3061 ccagccatcc agctatgtga gaacctgttc ttcctgtaac tgggaacttg gacaccagct
     3121 gcaaggagca actggattcc agctcagctc attcttacag aatcaacaga aatggcagtg
     3181 gagttttacg tttgcatttt ctctttctcc atccttgtag aattagattt ctgttcttct
     3241 tttaaccctg atattatagt agagtggtgt ggtacatgcc tgttactcca gcacatggga
     3301 gactgaagca gaaggatctt tagttcaagg ccagcctgga ctacatggaa agttccaagc
     3361 tagccaagat tatagagtga gaccctatct caaaagacaa aaccaaacca ctgtccccac
     3421 aaagcccaca acaaaaccaa agcctggtgt caaggaagca agtttctgag tagcagccag
     3481 cagctgcctt gatggagcag agtgatgtta gcagtgccgc tgctctgccg agcatgggcc
     3541 tacggacatg tccagtcccc ctaccaaata agtgaaggac ataaaaaaca gctcccaagg
     3601 cagcatgagc atcagtttat aaaccagagt ttatgcctac aagatcatcc tatttctggg
     3661 ccagaagtga catgaatgaa gtagtcattg cagatcttaa aggagagagt tactatgttc
     3721 agagagagtt agtgtttcag agagagcagc tgtttgcatg catggaagat gtaggaaaac
     3781 cagtgtaagg cacagtcagg tgagctgact tgacccaggc cagtgagttt ggccactcct
     3841 tggcctcctg agagtccagc tagagctgct tttctggacc ttttagtcat ttacgaccat
     3901 cagattgctt tcctgcagcc tgactgacac attcatgtgc acatacacac ttttgatctt
     3961 tgtgactaat gtgaaataaa ttcagttagg acattcagca gacctaggaa cattctggat
     4021 ggagtatagg tttttttctg caaaaatctg taaatgagat tatgcaagag tctcttccag
     4081 ctgtgggctg gtgttgcttg gaaattttca aaatcccaaa gtttcatgct tcccaaagtt
     4141 ggcttggaca aatgtgatgc tctcacctga gcctagacat gtaggaagtt ttcctagcgc
     4201 cagtgggctt ctgtatttgg gtgaagcact ggttttctgc gttttttttt tcccttctgt
     4261 ttcagaatct tcaagtttct tcaggcttaa tgaaccatcc cttgctttac tgggttttaa
     4321 aagctaattt tacttaacct cttcaaattt gtgtgtatct atttatgttt tggtgtggtg
     4381 gatggatggt aggggactga gcagaaattc agtgtgctag gagagcctca ctgggaagta
     4441 ctgaagagcc acagggtctg tgggagtcag tgttagcact caccaagcca ggccaggcct
     4501 tgggtttgaa ggcaacacca gcaaagcctt tagttcagta tagcacccag aggttggcga
     4561 gggataagtg ttggggtctt caaatacaac ccctggtgtt tggagtctgt gaccaaggcc
     4621 agactgagac ctgaaaggta aaagccatag gattaaacag agcagactga aaaatgagtc
     4681 tttaggttga acacattcca ctttctggat ttctctgtga gtattttgag tttgtcttta
     4741 gacgttgcta ttcctagcac tccttttctc tttaggtaga acagacactt aaccataatg
     4801 ccagaatgta ctttctatac ctgatgtcct tgccttgttt ttcagtttca gggccaggta
     4861 attgggccct gtggtgtaaa ataaaaacag tgtatgtgt
//
