LOCUS       NM_001415160            3426 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus pitrilysin metallopeptidase 1 (Pitrm1),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001415160 XM_006254186
VERSION     NM_001415160.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Brunetti,D., Torsvik,J., Dallabona,C., Teixeira,P.,
            Sztromwasser,P., Fernandez-Vizarra,E., Cerutti,R., Reyes,A.,
            Preziuso,C., D'Amati,G., Baruffini,E., Goffrini,P., Viscomi,C.,
            Ferrero,I., Boman,H., Telstad,W., Johansson,S., Glaser,E.,
            Knappskog,P.M., Zeviani,M. and Bindoff,L.A.
  TITLE     Defective PITRM1 mitochondrial peptidase is associated with Abeta
            amyloidotic neurodegeneration
  JOURNAL   EMBO Mol Med 8 (3), 176-190 (2016)
   PUBMED   26697887
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   King,J.V., Liang,W.G., Scherpelz,K.P., Schilling,A.B.,
            Meredith,S.C. and Tang,W.J.
  TITLE     Molecular basis of substrate recognition and degradation by human
            presequence protease
  JOURNAL   Structure 22 (7), 996-1007 (2014)
   PUBMED   24931469
REFERENCE   3  (bases 1 to 3426)
  AUTHORS   Pinho,C.M., Bjork,B.F., Alikhani,N., Backman,H.G., Eneqvist,T.,
            Fratiglioni,L., Glaser,E. and Graff,C.
  TITLE     Genetic and biochemical studies of SNPs of the mitochondrial A
            beta-degrading protease, hPreP
  JOURNAL   Neurosci Lett 469 (2), 204-208 (2010)
   PUBMED   19962426
REFERENCE   4  (bases 1 to 3426)
  AUTHORS   Chow,K.M., Gakh,O., Payne,I.C., Juliano,M.A., Juliano,L., Isaya,G.
            and Hersh,L.B.
  TITLE     Mammalian pitrilysin: substrate specificity and mitochondrial
            targeting
  JOURNAL   Biochemistry 48 (13), 2868-2877 (2009)
   PUBMED   19196155
REFERENCE   5  (bases 1 to 3426)
  AUTHORS   Falkevall,A., Alikhani,N., Bhushan,S., Pavlov,P.F., Busch,K.,
            Johnson,K.A., Eneqvist,T., Tjernberg,L., Ankarcrona,M. and
            Glaser,E.
  TITLE     Degradation of the amyloid beta-protein by the novel mitochondrial
            peptidasome, PreP
  JOURNAL   J Biol Chem 281 (39), 29096-29104 (2006)
   PUBMED   16849325
REFERENCE   6  (bases 1 to 3426)
  AUTHORS   Mzhavia,N., Berman,Y.L., Qian,Y., Yan,L. and Devi,L.A.
  TITLE     Cloning, expression, and characterization of human metalloprotease
            1: a novel member of the pitrilysin family of metalloendoproteases
  JOURNAL   DNA Cell Biol 18 (5), 369-380 (1999)
   PUBMED   10360838
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Dec 22, 2022 this sequence version replaced XM_006254186.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643291.39694.1,
                                           SRR26643290.50122.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760386,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                JAXUCZ010000017.1  68737284-68737350   c
            68-167              JAXUCZ010000017.1  68735802-68735901   c
            168-274             JAXUCZ010000017.1  68732545-68732651   c
            275-426             JAXUCZ010000017.1  68732056-68732207   c
            427-541             JAXUCZ010000017.1  68730309-68730423   c
            542-638             JAXUCZ010000017.1  68730129-68730225   c
            639-799             JAXUCZ010000017.1  68729032-68729192   c
            800-926             JAXUCZ010000017.1  68727845-68727971   c
            927-1015            JAXUCZ010000017.1  68727529-68727617   c
            1016-1144           JAXUCZ010000017.1  68725854-68725982   c
            1145-1258           JAXUCZ010000017.1  68725164-68725277   c
            1259-1355           JAXUCZ010000017.1  68724779-68724875   c
            1356-1490           JAXUCZ010000017.1  68723278-68723412   c
            1491-1629           JAXUCZ010000017.1  68721881-68722019   c
            1630-1746           JAXUCZ010000017.1  68720681-68720797   c
            1747-1879           JAXUCZ010000017.1  68718303-68718435   c
            1880-2000           JAXUCZ010000017.1  68716996-68717116   c
            2001-2077           JAXUCZ010000017.1  68716803-68716879   c
            2078-2243           JAXUCZ010000017.1  68716402-68716567   c
            2244-2344           JAXUCZ010000017.1  68715283-68715383   c
            2345-2465           JAXUCZ010000017.1  68712337-68712457   c
            2466-2537           JAXUCZ010000017.1  68711201-68711272   c
            2538-2650           JAXUCZ010000017.1  68710479-68710591   c
            2651-2776           JAXUCZ010000017.1  68708278-68708403   c
            2777-2922           JAXUCZ010000017.1  68707183-68707328   c
            2923-3025           JAXUCZ010000017.1  68706222-68706324   c
            3026-3426           JAXUCZ010000017.1  68705699-68706099   c
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.1"
     gene            1..3426
                     /gene="Pitrm1"
                     /note="pitrilysin metallopeptidase 1"
                     /db_xref="GeneID:307081"
                     /db_xref="RGD:1310998"
     exon            1..67
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..3119
                     /gene="Pitrm1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     pitrilysin metalloprotease 1; presequence protease,
                     mitochondrial; pitrilysin metallepetidase 1"
                     /codon_start=1
                     /product="presequence protease, mitochondrial isoform 2"
                     /protein_id="NP_001402089.1"
                     /db_xref="GeneID:307081"
                     /db_xref="RGD:1310998"
                     /translation="MWRFSGRRGLCAVQRLSSGVHHKVWREKSDQACDRALQYKVGEK
                     IHGFTVNQVTPVPELFLTAVKLSHDNTGARYLHLAREDNNNLFSVQFRTTPMDSTGVP
                     HVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAFTASDYTMYPFSTQNPKDFQNLLS
                     VYLDATFFPCLRELDFWQEGWRLEHEDPSDPQTPLIFKGVVFNEMKGAFTDNERIFSQ
                     HLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHTTHYHPSNARFFTYGNFPLEDHL
                     KQIHEEALSKFQKMEESTAVPAQKYWDKPREFHITCGPDSLATDATKQTTVSVSFLLP
                     DITNTFEAFTLNLLSSLLISGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGL
                     QGIAENDVKTVRELVDRTIEEVIEKGFEDDQIEALLHKIEIQMKHQSASFGMALTSYI
                     ASCWNHDGDPVELLQMGSQLTKFRKCLKENPKFLQEKVEQYFKNNPHRLTLSMKPDDR
                     YYEKQTQMETEKLEQKVNSLSQADKKQIYEKGLELQKQQSKHQDASCLPALKVSDIEP
                     TMPFTKFDIALSAGDVPVQYCPQPTNGIVYFRAFSSLNTLPEELRPFVPLFCTVLTKL
                     GCGILNYREQAQQIELKTGGMTVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHL
                     WSEIFNNPCFEEEEHFKVLVRMSAQELSNGIPDSGHLYAALRAGKTLTPAGDLQETFS
                     GMDQVKVMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENF
                     LRNVGRSKKERKPVRPHIVEKPTPSGPSGGAHADGSQIIRKLITDPTFKPCQMKTHFV
                     LPFPVNYVGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKVTHTG
                     IFTLYSYRDPNSIETLQSFGKAIDWAKSGKFTQQDIDEAKLSVFSAVDSPVAPSDKGM
                     DHFLYGLSDEMKQTYREQLFAVTHDKLTSVSHKYLGIGKSTHGLAILGPENSKLAKDP
                     SWIIR"
     exon            68..167
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            168..274
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            275..426
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            427..541
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            542..638
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..799
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            800..926
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1015
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1144
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1258
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1355
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1490
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1629
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1746
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1879
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2000
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2077
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2243
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2344
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2465
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2537
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2650
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2776
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2922
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2923..3025
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            3026..3426
                     /gene="Pitrm1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtcacatgc catgtggcgc ttcagcggtc ggcggggact gtgcgctgta cagcgactga
       61 gcagcggggt ccaccacaaa gtgtggagag agaagagtga ccaagcctgt gatcgtgctc
      121 tgcagtataa agtgggggaa aaaatccacg ggttcaccgt caaccaggtc actcctgttc
      181 cggaactgtt cctgacagcg gtgaaactca gccatgacaa cacgggagcc agatacctgc
      241 acctggccag ggaagataac aataacttat tcagtgtaca gttccgcaca accccaatgg
      301 atagcaccgg agtcccccat gttctcgagc ataccgtgct ctgcggctct cagaagtacc
      361 catgcagaga tcctttcttc aaaatgctca acaggtccct gtccacattt atgaatgcct
      421 tcacagccag cgattacacc atgtatccgt tttccacaca aaatcccaaa gactttcaaa
      481 acctcctctc ggtgtatttg gatgccacct tcttcccgtg cttgagggaa ctggacttct
      541 ggcaggaagg atggcgacta gagcatgagg atccgagcga ccctcagacg cccttgatct
      601 tcaagggggt cgtcttcaac gagatgaaag gggcgtttac agacaacgag aggatattct
      661 cccagcacct ccagaacaag cttcttcctg accacacata ctctgtggtt tctggagggg
      721 acccactgtg catccctgag ctcacgtggg agcagctgaa acagttccac acaactcact
      781 atcacccaag caatgccagg ttcttcactt atggaaattt tccgctggaa gatcacctga
      841 agcaaattca tgaggaagcc ctgagtaaat ttcagaaaat ggaggagagc acagcggtgc
      901 ctgcccagaa gtactgggac aagcctaggg aattccatat aacgtgtggc ccagattcac
      961 tagctacaga tgctaccaag caaacaactg tcagcgttag cttcctctta ccagatatca
     1021 ccaacacctt tgaagccttc accttgaacc ttctgtcttc cctcctgatt tccggcccta
     1081 actccccttt ctacaaagct ttgattgagt ctgggcttgg cacagacttc tctcccgacg
     1141 ttggatataa cggctacaca cgggaggctt acttcagcgt cgggctccag gggattgcag
     1201 agaacgatgt caagacggtc agagagctcg tagacaggac aattgaagaa gttatagaga
     1261 aaggttttga agatgatcag attgaagcac tgcttcataa aatcgagatc caaatgaagc
     1321 atcagtcggc cagttttggc atggccctga catcatatat agcttcttgc tggaatcatg
     1381 atggggaccc cgtggagctc ctgcaaatgg gaagtcagct gactaaattt aggaagtgcc
     1441 ttaaggaaaa tccaaaattt ttacaagaaa aagtagagca gtattttaag aacaatccgc
     1501 acaggctgac tttatccatg aaaccagatg acaggtatta cgaaaagcaa actcagatgg
     1561 agacggaaaa gctagagcag aaggtgaatt ctctctccca ggcggacaaa aagcagatct
     1621 acgagaaagg tttagaattg cagaagcagc aaagtaaaca tcaagacgcc tcctgtctcc
     1681 cggcactaaa agtctctgac atcgagccca ccatgccttt caccaagttt gacatcgccc
     1741 tctcagctgg agacgtccct gtgcagtact gcccgcagcc caccaacggc attgtgtatt
     1801 tccgagcctt ttccagttta aacacgctgc cggaggagtt gagacccttc gtgcctctct
     1861 tctgcactgt gctgaccaag ctgggttgtg gcatcctcaa ctacagagag caagctcaac
     1921 agatcgagct gaagacggga ggcatgaccg tcacccccca tgtgctccct gatgactcgc
     1981 agctggacac ctacgagcag ggtgtgttat tttcatccct ctgcctggag cggaacctgc
     2041 cagacatgat gcatctgtgg agcgaaatat ttaacaaccc atgctttgaa gaagaagagc
     2101 acttcaaagt gttggtgagg atgagcgctc aggagctctc caacgggatc ccagactcgg
     2161 ggcatctcta cgcagccctc agagcaggca agacactcac gcctgcaggg gacttgcagg
     2221 agaccttcag tgggatggat caggtgaagg tgatgaaaag aattgccgaa atgacagaca
     2281 tcaagcccat cctgagaaaa ctaccccgga tcaagaagta tctactaaac tgtgacaaca
     2341 tgagatgctc agtgaatgct acccctcagc agatgcctca ggcagagaaa gaggtggaaa
     2401 acttccttag aaacgtcggc cgaagcaaaa aggaacggaa gcctgtccgc ccccatattg
     2461 tcgagaaacc cacacccagt ggccccagtg gaggtgccca tgccgatggg tcccagatca
     2521 tcagaaagtt gataacagac cccaccttca agccgtgcca gatgaagaca catttcgtgc
     2581 tgcccttccc ggtgaattac gttggcgagt gtgtcagaac cgtcccctat gccgatccag
     2641 atcatgccag ccttaagatc cttgcccgtt taatgacagc taaattcttg catacggaaa
     2701 ttcgagaaaa ggggggtgct tatggtggag gtgctaaagt cacccacacc gggatcttca
     2761 cgctctactc ttacagggat ccaaactcca tagaaacact acagtctttt gggaaggcta
     2821 tagactgggc taaatctgga aagttcacac agcaggacat tgatgaagcc aagctttccg
     2881 ttttctccgc tgtggattct cctgttgctc catcggataa aggaatggac cacttcctgt
     2941 acggcctctc cgatgagatg aagcagacat accgggaaca gctcttcgct gtcacccacg
     3001 acaagctgac ctctgtgagc cataaatacc tcggcatcgg gaagagcacg cacggcctgg
     3061 ctattctggg accagagaac tcaaaactcg ccaaagaccc atcgtggatc ataagatgaa
     3121 tatctttgag tatacgtaag aagtcagatg ctcttaaaaa ctgagcctct gagctagatt
     3181 taatgtagat ggatcacaag agttactggt ttctttgatc caaaagccat tttcatatga
     3241 aaatccaacc aaaggtattt actgactggc aagttgggca aaaacgctta aggaaatact
     3301 gttaccatga agaaattcat taatcatgca ttaaatgaca acagtgagca aattaagctt
     3361 ctgaaacatt ctgaaacatt actaataaaa atatttattt taaaaataaa agccaactcc
     3421 aaccta
//
