LOCUS       NM_017231               1638 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus phosphatidylinositol transfer protein, alpha
            (Pitpna), mRNA.
ACCESSION   NM_017231
VERSION     NM_017231.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Grabon,A., Orlowski,A., Tripathi,A., Vuorio,J., Javanainen,M.,
            Rog,T., Lonnfors,M., McDermott,M.I., Siebert,G., Somerharju,P.,
            Vattulainen,I. and Bankaitis,V.A.
  TITLE     Dynamics and energetics of the mammalian phosphatidylinositol
            transfer protein phospholipid exchange cycle
  JOURNAL   J Biol Chem 292 (35), 14438-14455 (2017)
   PUBMED   28718450
  REMARK    GeneRIF: Data suggest that (1) interaction of Pitpna with
            membrane/lipid bilayer is spontaneous and mediated by four specific
            protein substructures; (2) ability of Pitpna to initiate closure
            around PC ligand is accompanied by loss of flexibility of two
            helix/loop regions, as well as of C-terminal helix; (3) energy
            barrier of PC/PI extraction from membrane is lowered by hydrogen
            bonding between lipid and Pitpna.
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Garner,K., Hunt,A.N., Koster,G., Somerharju,P., Groves,E., Li,M.,
            Raghu,P., Holic,R. and Cockcroft,S.
  TITLE     Phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1)
            binds and transfers phosphatidic acid
  JOURNAL   J Biol Chem 287 (38), 32263-32276 (2012)
   PUBMED   22822086
REFERENCE   3  (bases 1 to 1638)
  AUTHORS   Shadan,S., Holic,R., Carvou,N., Ee,P., Li,M., Murray-Rust,J. and
            Cockcroft,S.
  TITLE     Dynamics of lipid transfer by phosphatidylinositol transfer
            proteins in cells
  JOURNAL   Traffic 9 (10), 1743-1756 (2008)
   PUBMED   18636990
REFERENCE   4  (bases 1 to 1638)
  AUTHORS   Kang,D., Oh,S., Ahn,S.M., Lee,B.H. and Moon,M.H.
  TITLE     Proteomic analysis of exosomes from human neural stem cells by flow
            field-flow fractionation and nanoflow liquid chromatography-tandem
            mass spectrometry
  JOURNAL   J Proteome Res 7 (8), 3475-3480 (2008)
   PUBMED   18570454
REFERENCE   5  (bases 1 to 1638)
  AUTHORS   Werner,H.B., Kuhlmann,K., Shen,S., Uecker,M., Schardt,A.,
            Dimova,K., Orfaniotou,F., Dhaunchak,A., Brinkmann,B.G., Mobius,W.,
            Guarente,L., Casaccia-Bonnefil,P., Jahn,O. and Nave,K.A.
  TITLE     Proteolipid protein is required for transport of sirtuin 2 into CNS
            myelin
  JOURNAL   J Neurosci 27 (29), 7717-7730 (2007)
   PUBMED   17634366
REFERENCE   6  (bases 1 to 1638)
  AUTHORS   Tremblay,J.M., Li,H., Yarbrough,L.R. and Helmkamp,G.M. Jr.
  TITLE     Modifications of cysteine residues in the solution and
            membrane-associated conformations of phosphatidylinositol transfer
            protein have differential effects on lipid transfer activity
  JOURNAL   Biochemistry 40 (31), 9151-9158 (2001)
   PUBMED   11478882
  REMARK    GeneRIF: Cys-to-Ala mutations of PITP show that neither Cys95 nor
            Cys188 is essential for lipid transfer activity; however, chemical
            modification of Cys95 results in the loss of lipid transfer
            activity.
REFERENCE   7  (bases 1 to 1638)
  AUTHORS   Yoder,M.D., Thomas,L.M., Tremblay,J.M., Oliver,R.L., Yarbrough,L.R.
            and Helmkamp,G.M. Jr.
  TITLE     Structure of a multifunctional protein. Mammalian
            phosphatidylinositol transfer protein complexed with
            phosphatidylcholine
  JOURNAL   J Biol Chem 276 (12), 9246-9252 (2001)
   PUBMED   11104777
REFERENCE   8  (bases 1 to 1638)
  AUTHORS   Alb,J.G. Jr., Gedvilaite,A., Cartee,R.T., Skinner,H.B. and
            Bankaitis,V.A.
  TITLE     Mutant rat phosphatidylinositol/phosphatidylcholine transfer
            proteins specifically defective in phosphatidylinositol transfer:
            implications for the regulation of phospholipid transfer activity
  JOURNAL   Proc Natl Acad Sci U S A 92 (19), 8826-8830 (1995)
   PUBMED   7568025
REFERENCE   9  (bases 1 to 1638)
  AUTHORS   Dickeson,S.K., Lim,C.N., Schuyler,G.T., Dalton,T.P., Helmkamp,G.M.
            Jr. and Yarbrough,L.R.
  TITLE     Isolation and sequence of cDNA clones encoding rat
            phosphatidylinositol transfer protein
  JOURNAL   J Biol Chem 264 (28), 16557-16564 (1989)
   PUBMED   2777797
REFERENCE   10 (bases 1 to 1638)
  AUTHORS   Funkhouser,J.D.
  TITLE     Amino-terminal sequence of a phospholipid transfer protein from rat
            lung
  JOURNAL   Biochem Biophys Res Commun 145 (3), 1310-1314 (1987)
   PUBMED   3606604
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from M25758.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M25758.1, BC070945.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     gene            1..1638
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /note="phosphatidylinositol transfer protein, alpha"
                     /db_xref="GeneID:29525"
                     /db_xref="RGD:61885"
     exon            1..212
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..1008
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /note="PI-TP-alpha; ptdIns transfer protein alpha;
                     ptdInsTP alpha"
                     /codon_start=1
                     /product="phosphatidylinositol transfer protein alpha
                     isoform"
                     /protein_id="NP_058927.1"
                     /db_xref="GeneID:29525"
                     /db_xref="RGD:61885"
                     /translation="MVLLKEYRVILPVSVDEYQVGQLYSVAEASKNETGGGEGVEVLV
                     NEPYEKDDGEKGQYTHKIYHLQSKVPTFVRMLAPEGALNIHEKAWNAYPYCRTVITNE
                     YMKEDFLIKIETWHKPDLGTQENVHKLEPEAWKHVEAIYIDIADRSQVLSKDYKAEED
                     PAKFKSIKTGRGPLGPNWKQELVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQE
                     KRLFTNFHRQLFCWLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD"
     exon            213..243
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            244..392
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            393..484
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            485..492
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            493..567
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            568..651
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            652..729
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            730..840
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            841..963
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1029
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1638
                     /gene="Pitpna"
                     /gene_synonym="Pitpn"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgggaggtg gcgaggccag ggcagggtgg cagaagcccg cgcggcgacc gcagcgagag
       61 cagcggggac agagcagacc acaacgaagg cgcacaggca gcggggccgg gccgggccac
      121 cgggagagcg gccaccggga agcgggcggg aggccgggct ggcagctggc agccgccgag
      181 ccgcgaagcg acatggtgct gctcaaggaa tatcgggtca tcctgcctgt gtctgtagat
      241 gagtatcaag tggggcagct gtactctgtg gctgaagcca gtaaaaatga aactggtggt
      301 ggggaaggtg tggaggtcct ggtgaacgag ccctacgaga aggatgatgg cgagaaaggc
      361 cagtacacac acaagatcta ccacttacag agcaaagttc ccacgtttgt tcgaatgctg
      421 gccccagaag gcgccctgaa tatacatgag aaagcctgga atgcctaccc ttactgcaga
      481 accgttatta caaatgagta catgaaggaa gactttctca ttaaaattga aacctggcac
      541 aagccagacc ttggcaccca ggagaatgtg cataaactgg agcctgaggc atggaaacat
      601 gtggaagcta tatatataga catcgctgat cgaagccaag tacttagcaa ggattacaag
      661 gcagaggaag acccagcaaa atttaaatct atcaaaacag gacgaggacc attgggcccg
      721 aattggaagc aagaacttgt caatcagaag gactgcccat atatgtgtgc atacaaactg
      781 gttactgtca agttcaagtg gtggggcttg cagaacaaag tggaaaactt tatacataag
      841 caagagaagc gtctgtttac aaactttcac aggcagctgt tctgttggct tgataaatgg
      901 gttgatctga ctatggatga cattcggagg atggaagaag agacgaagag acagctggat
      961 gagatgagac aaaaggaccc cgtgaaagga atgacagcag atgactagcg ctaccgccct
     1021 ttctgcactt tttgcaagac agtggtccac aggaaggccc agcagctcca ccctcctgag
     1081 tggcaggcca tctttggacg cagcctttct gtgctcattc ttcaggcaac tttcagtccc
     1141 ttactatagc tatttctaga tgccctttaa cattgtgaac aaatagaccg tctggtatta
     1201 taaagcctgt gtgtgcggga gcctgatcct ctaagatata tggtgtatgc tgctgtattt
     1261 acagctcatc ctctctccat tgtgccctga cccatttctg tatgccctcc ccctttattt
     1321 ccaagtgacg ttctcattct ttcaaatgtg ctgcctttgt gatgtgatta tttaagctca
     1381 cttatttcag ccttgggata agctgagcta ttttgcttcc tgggcagatc tttggcaatg
     1441 ttgaatattc acttggggag agaggaggag ttaaaaatac agagacacct accctagctc
     1501 ctcacaatag gaatgtggct tcagaaccct gacctctgct cctagtaacc tcttgttcgc
     1561 gtgctctaag caccaagaag gcacccaagg cccagccagt ctaagaaaat ttagcaaata
     1621 ataaataacc cctactcg
//
