LOCUS       NM_053926               3507 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus phosphatidylinositol-5-phosphate 4-kinase type 2
            alpha (Pip4k2a), mRNA.
ACCESSION   NM_053926
VERSION     NM_053926.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Clarke,J.H., Giudici,M.L., Burke,J.E., Williams,R.L., Maloney,D.J.,
            Marugan,J. and Irvine,R.F.
  TITLE     The function of phosphatidylinositol 5-phosphate 4-kinase gamma
            (PI5P4Kgamma) explored using a specific inhibitor that targets the
            PI5P-binding site
  JOURNAL   Biochem J 466 (2), 359-367 (2015)
   PUBMED   25495341
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Vicinanza,M., Korolchuk,V.I., Ashkenazi,A., Puri,C., Menzies,F.M.,
            Clarke,J.H. and Rubinsztein,D.C.
  TITLE     PI(5)P regulates autophagosome biogenesis
  JOURNAL   Mol Cell 57 (2), 219-234 (2015)
   PUBMED   25578879
REFERENCE   3  (bases 1 to 3507)
  AUTHORS   Clarke,J.H. and Irvine,R.F.
  TITLE     Evolutionarily conserved structural changes in phosphatidylinositol
            5-phosphate 4-kinase (PI5P4K) isoforms are responsible for
            differences in enzyme activity and localization
  JOURNAL   Biochem J 454 (1), 49-57 (2013)
   PUBMED   23758345
REFERENCE   4  (bases 1 to 3507)
  AUTHORS   Schulze,H., Korpal,M., Hurov,J., Kim,S.W., Zhang,J., Cantley,L.C.,
            Graf,T. and Shivdasani,R.A.
  TITLE     Characterization of the megakaryocyte demarcation membrane system
            and its role in thrombopoiesis
  JOURNAL   Blood 107 (10), 3868-3875 (2006)
   PUBMED   16434494
REFERENCE   5  (bases 1 to 3507)
  AUTHORS   Ishihara,H., Shibasaki,Y., Kizuki,N., Wada,T., Yazaki,Y., Asano,T.
            and Oka,Y.
  TITLE     Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the
            third isoform and deletion/substitution analysis of members of this
            novel lipid kinase family
  JOURNAL   J Biol Chem 273 (15), 8741-8748 (1998)
   PUBMED   9535851
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB032899.1, CV077559.1, CB715008.1, CO573943.1, DV713604.1 and
            BQ782736.1.
            
            On May 23, 2007 this sequence version replaced NM_053926.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1530              AB032899.1         1-1530
            1531-2211           CV077559.1         71-751
            2212-2426           CB715008.1         208-422
            2427-2717           CO573943.1         169-459
            2718-3438           DV713604.1         42-762
            3439-3507           BQ782736.1         1-69                c
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.3"
     gene            1..3507
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /note="phosphatidylinositol-5-phosphate 4-kinase type 2
                     alpha"
                     /db_xref="GeneID:116723"
                     /db_xref="RGD:621708"
     exon            1..240
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..1317
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /EC_number="2.7.1.149"
                     /note="1-phosphatidylinositol-5-phosphate 4-kinase A;
                     PIPK2 alpha; PIP4KII-alpha; diphosphoinositide kinase
                     2-alpha; PI(5)P 4-kinase type II alpha;
                     phosphatidylinositol-5-phosphate 4-kinase type-2
                     alpha-like; phosphatidylinositol-4-phosphate 5-kinase,
                     type II, alpha; 1-phosphatidylinositol 5-phosphate
                     4-kinase 2-alpha; phosphatidylinositol 5-phosphate
                     4-kinase type II alpha"
                     /codon_start=1
                     /product="phosphatidylinositol 5-phosphate 4-kinase type-2
                     alpha"
                     /protein_id="NP_446378.1"
                     /db_xref="GeneID:116723"
                     /db_xref="RGD:621708"
                     /translation="MATPSNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWG
                     VNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLR
                     ERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYVIKTITSEDVAEMHNILK
                     KYHQYIVECHGVTLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVA
                     REASDKEKAKELPTLKDNDFINEGQKIYIDDSNKKIFLEKLKKDVEFLAQLKLMDYSL
                     LVGIHDVERAEQEEVECEENEGEEEGESDGAHPIGTPPDSPGNTLNSSPPLAPGEFDP
                     NIDVYAIKCHENAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQ
                     YSKRFLDFIGHILT"
     exon            241..338
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            339..435
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            436..588
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            589..735
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            736..774
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            775..888
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1132
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1236
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..3490
                     /gene="Pip4k2a"
                     /gene_synonym="Pip5k2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcccc gggggatgcg gctgcctccc taggctggcg tgtagggagg gcgggtcccc
       61 ggcctcggcg gcgcggcggt ggaggggaca gcggccatgg cgacccctag caacctgggg
      121 tcctccgtcc tggcgagcaa gaccaagacg aagaagaagc acttcgtggc tcagaaagtg
      181 aagctgttcc gggccagcga cccgctgctc agcgtgctca tgtggggggt caaccactcg
      241 atcaatgaac tgagccatgt tcaaatccct gtcatgttga tgcctgatga cttcaaagcc
      301 tactcaaaga taaaggtgga caaccacctt ttcaacaagg aaaacatgcc gagccatttc
      361 aagtttaagg aatactgccc gatggtcttc cggaatcttc gggagaggtt tggaatcgac
      421 gaccaagatt tccagaattc cttgaccaga agtgcgcccc ttcccaatga ctcgcaggct
      481 cgcagcgggg ctcggtttca cacgtcctat gacaaaagat acgtcatcaa gaccattacc
      541 agcgaggacg tggcagagat gcacaacatt ctcaagaagt accaccagta tatagtggaa
      601 tgccatgggg tcaccctcct tcctcagttc ttgggaatgt accggcttaa tgtcgacgga
      661 gtggaaatat acgtgattgt tacgaggaac gtgttcagcc accgtctctc tgtgtatagg
      721 aagtacgacc tgaagggctc gacggtggct agagaagcta gtgacaaaga aaaggccaaa
      781 gagcttccaa ctttaaagga caatgacttc atcaatgagg gccaaaagat ttatatcgat
      841 gacagcaaca aaaagatatt cctggaaaaa ctgaaaaagg atgtcgagtt ccttgcacaa
      901 ctaaaactca tggactacag cctcctggtg gggattcatg atgtggagcg agcagagcaa
      961 gaggaggttg agtgtgagga gaacgagggg gaagaagaag gagagagtga cggtgcccac
     1021 cccatcggga cgccacctga cagtcctgga aacacactca acagctcacc acccttggct
     1081 cctggcgagt tcgacccaaa cattgatgtt tatgcaatta aatgccatga aaacgcacct
     1141 aggaaagaag tgtacttcat ggcgattatt gacattctca ctcattacga tgcaaaaaag
     1201 aaagctgccc acgctgcgaa aactgtaaaa cacggcgctg gagccgagat ctcgaccgtg
     1261 aacccagagc agtattcaaa gcgctttttg gactttatcg gacacatctt gacgtaacct
     1321 cctgcatagt cccggatggg cacgagctcg ggaaggctgg tggcggcttc ggtgtaggaa
     1381 taaaaaaaag gaaaatggaa ctccacgaag cactcacctt gcaggaagca aacccttgtt
     1441 tacatcttca agccgggatg actgaagttg ggggctcctc gctttaacag ctacccgata
     1501 tttcccagcg ccgccgctac ttctgactct gtgtgtgcgc tcgctcgcac tcgtgtgtgt
     1561 gcacacacac gggcgcactc tctcttgtgc acattttaaa gtaattaatt aattaataat
     1621 taattaaaac cgatcagctt gaatcagagc ttgtaacagc cagacccctc tgattccggc
     1681 tgtggcagca tggatggatg aagagacaag ctgggggagg ggaccagaaa tgcacctcat
     1741 cgcagcacca ccagagaagc tgtcgatcag tttgttttct gacttgaaag ataggaaatt
     1801 gttcatcatg gtggtggtgg tggtggtgat gatggtggtg atgatgatga ttaaaagaaa
     1861 gaaaaattta tatcaaatgt tacactcctg gcccacagaa agacctctcc tgaatagtgg
     1921 ttgtctaagc ccacacacag ccctaggtaa aggaatgcta gcatctccct gtgtactggt
     1981 tcaggtttct ctccgaacag tgtcactatc cccccgttgt cccaggctga gcccttcatc
     2041 ttccaagagt ctgatgccaa gaacccgtct acagtttcac ctccaggaaa acccgcttgc
     2101 ttctcttttc tcacctggag cctggcgttg ttaacaccct catgtccggc tgcgggaatt
     2161 cactctgcaa aacagatgta gaacgtccct gaagggtgca tagtttgggg caattgcagc
     2221 tgcacctccc accacaggaa gatgagcaag aagttaaaac ccccctgtga cccaaggcaa
     2281 ttaatcccaa ttatacaagg tttctgttta accttggttt aggaattgaa tttgctatga
     2341 attatccctt taactgaaac cctcagtttt actgtttaca tggttagaga aacagggatc
     2401 tttttgaatc taaaaattta atacacagca cgtgattttt gaagtttaca tgtaaagcca
     2461 ttttgcagtg tagggagcat aatgtttgtt gtgttttgag atatgcgtct tgcagttgtg
     2521 tttctgagtg agtgttctgt tcaaagtact tagtagacag tctatcacat aaaacagaag
     2581 gattctcttc ctcaagatgt gtatttatct gccttatggt tttttttttc ctataaaaag
     2641 attaaaccca aaatctggtt attgtaagtt cattgcccta aactgtgctg atggtgtgta
     2701 actgagactt agagaaaaag aaaaaaagga aaccttccgg catcaattgt gtatctacca
     2761 acatccctgc attcctagaa ggcctcaagt tacagtccta gatttgctca gagcacatta
     2821 ccaacatacg ggctgtgctg cagccgtgcc tttaaaagcc ccgcctaaag tacaagatgg
     2881 tttccgcagg cagttttaga aaggaaaaaa aaatccaccc ttcaataatt acaagtcaac
     2941 aagtcccctg ccaggctgcc ttccctgtca ctttactttt ggcctctcca gatacagctg
     3001 tgccccccgt gtgtgggctg ccctcacagc ctctggaagg cagcatcaac accatctgac
     3061 tcctgtgtac tgacccacca caggttcctt agctgatgat gttaactcca ctgtaacata
     3121 gctgtgcaaa tctgcatttg atgcatttaa aataatcaat gctgtgtgat tcggtttctg
     3181 ggcactgtta cacggcgttg tggtgtcccg atccgtttga gtgttccaga agcttctact
     3241 gttaaccttt gatccccaaa cttataaacc caaaggaaaa tgaaggtaca cattcatgat
     3301 ttgagtaacc atttcctctg tggccaatct gtatgctttt agaagtttaa aaaaaaatgc
     3361 tttggtcttt gtctgtaacc atctctgtca tccaaataag ccgtttggac aagagtgaga
     3421 acagtgctaa cagtgcgctc tcgaggcgtg agccgggctt tacgaataaa gcactttgat
     3481 gactctctta aaaaaaaaaa aaaaaaa
//
