LOCUS       NM_133399               6418 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus phosphatidylinositol-4,5-bisphosphate 3-kinase,
            catalytic subunit alpha (Pik3ca), mRNA.
ACCESSION   NM_133399 XM_001059350 XM_001063573 XM_574916
VERSION     NM_133399.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Wang,S., Duan,J., Liao,J., Wang,Y., Xiao,X., Li,L., Liu,Y., Gu,H.,
            Yang,P., Fu,D., Du,J., Li,X. and Shao,M.
  TITLE     LncRNA H19 inhibits ER stress induced apoptosis and improves
            diabetic cardiomyopathy by regulating PI3K/AKT/mTOR axis
  JOURNAL   Aging (Albany NY) 14 (16), 6809-6828 (2022)
   PUBMED   36044268
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Gao,H., Du,W.Y., Lin,J., Han,S.L., Zhang,Y.J. and Sun,X.F.
  TITLE     Losartan Protects Podocytes against High Glucose-induced Injury by
            Inhibiting B7-1 Expression
  JOURNAL   Curr Med Sci 41 (3), 505-512 (2021)
   PUBMED   34129205
  REMARK    GeneRIF: Losartan Protects Podocytes against High Glucose-induced
            Injury by Inhibiting B7-1 Expression.
REFERENCE   3  (bases 1 to 6418)
  AUTHORS   Beca,F., Krings,G., Chen,Y.Y., Hosfield,E.M., Vohra,P.,
            Sibley,R.K., Troxell,M.L., West,R.B., Allison,K.H. and Bean,G.R.
  TITLE     Primary mammary angiosarcomas harbor frequent mutations in KDR and
            PIK3CA and show evidence of distinct pathogenesis
  JOURNAL   Mod Pathol 33 (8), 1518-1526 (2020)
   PUBMED   32123305
REFERENCE   4  (bases 1 to 6418)
  AUTHORS   Seo,A.N., Kang,B.W., Bae,H.I., Kwon,O.K., Park,K.B., Lee,S.S.,
            Chung,H.Y., Yu,W., Jeon,S.W., Kang,H. and Kim,J.G.
  TITLE     Exon 9 Mutation of PIK3CA Associated With Poor Survival in Patients
            With Epstein-Barr Virus-associated Gastric Cancer
  JOURNAL   Anticancer Res 39 (4), 2145-2154 (2019)
   PUBMED   30952761
REFERENCE   5  (bases 1 to 6418)
  AUTHORS   Zhou,H., Tan,S., Li,H. and Lin,X.
  TITLE     Expression and significance of EBV, ARID1A and PIK3CA in gastric
            carcinoma
  JOURNAL   Mol Med Rep 19 (3), 2125-2136 (2019)
   PUBMED   30747208
REFERENCE   6  (bases 1 to 6418)
  AUTHORS   Lu,W., Bhasin,M. and Tsirka,S.E.
  TITLE     Involvement of tissue plasminogen activator in onset and effector
            phases of experimental allergic encephalomyelitis
  JOURNAL   J Neurosci 22 (24), 10781-10789 (2002)
   PUBMED   12486171
REFERENCE   7  (bases 1 to 6418)
  AUTHORS   Hers,I., Bell,C.J., Poole,A.W., Jiang,D., Denton,R.M., Schaefer,E.
            and Tavare,J.M.
  TITLE     Reciprocal feedback regulation of insulin receptor and insulin
            receptor substrate tyrosine phosphorylation by phosphoinositide
            3-kinase in primary adipocytes
  JOURNAL   Biochem J 368 (Pt 3), 875-884 (2002)
   PUBMED   12220227
  REMARK    GeneRIF: PI 3-kinase feedback control of signalling by the insulin
            receptor and IRS proteins is multifaceted and reciprocal
REFERENCE   8  (bases 1 to 6418)
  AUTHORS   Cros,N., Tkatchenko,A.V., Pisani,D.F., Leclerc,L., Leger,J.J.,
            Marini,J.F. and Dechesne,C.A.
  TITLE     Analysis of altered gene expression in rat soleus muscle atrophied
            by disuse
  JOURNAL   J Cell Biochem 83 (3), 508-519 (2001)
   PUBMED   11596118
REFERENCE   9  (bases 1 to 6418)
  AUTHORS   Pons,S., Asano,T., Glasheen,E., Miralpeix,M., Zhang,Y.,
            Fisher,T.L., Myers,M.G. Jr., Sun,X.J. and White,M.F.
  TITLE     The structure and function of p55PIK reveal a new regulatory
            subunit for phosphatidylinositol 3-kinase
  JOURNAL   Mol Cell Biol 15 (8), 4453-4465 (1995)
   PUBMED   7542745
REFERENCE   10 (bases 1 to 6418)
  AUTHORS   Carpenter,C.L., Duckworth,B.C., Auger,K.R., Cohen,B.,
            Schaffhausen,B.S. and Cantley,L.C.
  TITLE     Purification and characterization of phosphoinositide 3-kinase from
            rat liver
  JOURNAL   J Biol Chem 265 (32), 19704-19711 (1990)
   PUBMED   2174051
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000067.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_133399.4.
            
            On Nov 27, 2020 this sequence version replaced NM_133399.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data from several strains because no single
            transcript was available for the full length of the gene. The
            extent of this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132262,
                              SAMD00132263 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-352               JACYVU010000067.1  30286542-30286893
            353-562             JACYVU010000067.1  30287429-30287638
            563-813             JACYVU010000067.1  30288135-30288385
            814-1059            JACYVU010000067.1  30290045-30290290
            1060-1145           JACYVU010000067.1  30290970-30291055
            1146-1251           JACYVU010000067.1  30293209-30293314
            1252-1404           JACYVU010000067.1  30293956-30294108
            1405-1539           JACYVU010000067.1  30294187-30294321
            1540-1664           JACYVU010000067.1  30298759-30298883
            1665-1746           JACYVU010000067.1  30300273-30300354
            1747-1911           JACYVU010000067.1  30300607-30300771
            1912-2015           JACYVU010000067.1  30300956-30301059
            2016-2187           JACYVU010000067.1  30302487-30302658
            2188-2294           JACYVU010000067.1  30304809-30304915
            2295-2416           JACYVU010000067.1  30305314-30305435
            2417-2495           JACYVU010000067.1  30306859-30306937
            2496-2666           JACYVU010000067.1  30310521-30310691
            2667-2784           JACYVU010000067.1  30312143-30312260
            2785-2936           JACYVU010000067.1  30312361-30312512
            2937-6418           JACYVU010000067.1  30313175-30316656
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     gene            1..6418
                     /gene="Pik3ca"
                     /note="phosphatidylinositol-4,5-bisphosphate 3-kinase,
                     catalytic subunit alpha"
                     /db_xref="GeneID:170911"
                     /db_xref="RGD:620916"
     CDS             1..3207
                     /gene="Pik3ca"
                     /EC_number="2.7.1.137"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.1.153"
                     /note="phosphatidylinositol 3-kinase, catalytic, alpha
                     polypeptide; phosphatidylinositol 4,5-bisphosphate
                     3-kinase 110 kDa catalytic subunit alpha; PI3Kalpha;
                     p110alpha; PI3K-alpha; PI3-kinase subunit alpha;
                     ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit
                     p110-alpha; serine/threonine protein kinase PIK3CA;
                     phosphoinositide-3-kinase catalytic alpha polypeptide"
                     /codon_start=1
                     /product="phosphatidylinositol 4,5-bisphosphate 3-kinase
                     catalytic subunit alpha isoform"
                     /protein_id="NP_596890.2"
                     /db_xref="GeneID:170911"
                     /db_xref="RGD:620916"
                     /translation="MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVT
                     IKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVI
                     EPVGNREEKILNREIGFVIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPH
                     SRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPE
                     QVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSC
                     IMLGRMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGETATKSLWVINSAL
                     RIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIY
                     IPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPH
                     GLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSY
                     SHTGLSNRLARDNELRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILP
                     KLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLT
                     DDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQ
                     RFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMR
                     QPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNE
                     IIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHT
                     IMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIG
                     DRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYT
                     KTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDK
                     TEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN"
     exon            1..352
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            353..562
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            563..813
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            814..1059
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1145
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1251
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1404
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1539
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1664
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1746
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1911
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2015
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2187
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2294
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2416
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2495
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2496..2666
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2784
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2936
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
     exon            2937..6418
                     /gene="Pik3ca"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgcctccac gaccatcttc gggtgaactg tggggcatcc acttgatgcc cccacgaatc
       61 ctagtggaat gtttactccc aaatggaatg atagtgactt tagaatgcct ccgtgaggcc
      121 acactagtca ccatcaagca tgaactgttc aaagaggcca ggaaataccc tctccatcag
      181 cttctgcaag atgaatcatc ttacattttc gtaagtgtta cccaagaagc agaaagggaa
      241 gaatttttcg atgaaacaag acggctttgt gaccttcggc tttttcaacc ctttttaaaa
      301 gtaattgagc cagtaggcaa ccgtgaagaa aagatcctca accgagaaat tggttttgtt
      361 attggcatgc cagtgtgtga atttgatatg gttaaagatc cagaagtcca agacttccga
      421 aggaacattc tgaatgtttg caaagaagcc gtggacctgc gggatctcaa ctcgcctcat
      481 agcagagcaa tgtatgtcta ccctccaaat gtcgagtctt ccccagaact gccaaagcac
      541 atctacaaca agttagataa aggacaaatc atagtggtga tttgggtgat agtctctcca
      601 aacaacgaca agcagaagta cactctgaag atcaaccatg actgcgtgcc agagcaagtc
      661 attgctgagg ccatcaggaa gaagacccgg agcatgttgc tgtcctcgga gcagctgaaa
      721 ctctgtgtct tagaatacca gggcaagtac attctcaaag tgtgtggctg tgatgagtac
      781 ttcctagaga agtaccctct gagtcagtac aagtacataa gaagctgtat aatgctgggg
      841 aggatgccca acttgatgct gatggccaag gagagcctgt actctcagct gccgatcgat
      901 agcttcacaa tgccatccta ctccaggcgc atttccacag cgacacccta tatgaacggg
      961 gagactgcta cgaaatccct ctgggttata aatagcgcgc tcagaataaa aattctgtgt
     1021 gcaacctatg taaatgtaaa tattcgagac attgataaga tctatgttcg aacaggtatc
     1081 taccatggag gagaaccctt atgtgacaat gtgaatactc aaagagtccc ttgttccaat
     1141 cctaggtgga atgaatggct gaattatgat atatacattc ctgatcttcc tcgtgctgcc
     1201 cgcctttgcc tttcaatctg ctctgttaaa ggccgaaagg gtgctaagga ggagcactgt
     1261 ccgttggcct ggggaaacat aaacttgttt gattatacag acaccctagt gtccgggaaa
     1321 atggctttga atctctggcc tgtaccacat gggttggaag atctgctgaa ccctattggt
     1381 gttactgggt caaatccaaa taaagaaact ccatgcttag agttggagtt tgattggttc
     1441 agcagtgtgg tgaagtttcc agatatgtct gtgatcgaag agcatgccaa ttggtctgtg
     1501 tcccgagaag ccggattcag ttactctcat acaggactga gtaacagact agccagagac
     1561 aatgagttaa gagaaaatga caaggaacag ctccgagcac tttgtacccg ggacccactg
     1621 tctgaaatca ctgaacaaga gaaagacttc ctatggagcc acagacacta ctgtgtaact
     1681 attcctgaaa tcctacccaa attgcttctg tctgtcaagt ggaattccag agatgaagtg
     1741 gcccagatgt actgcttagt aaaagattgg cctccaatca aaccagagca agccatggag
     1801 ctcctggact gtaactaccc agaccccatg gttcggagct ttgctgtccg gtgcttggaa
     1861 aaatacttaa cagatgacaa actttctcag tacctcatcc agcttgtaca ggtcttaaaa
     1921 tatgaacagt atttggataa cctgcttgtg agatttttac tcaagaaagc actgacaaat
     1981 caaaggattg gccatttttt cttttggcat ttaaaatctg agatgcacaa taagactgtc
     2041 agtcagaggt tcggcctgct gttggagtcc tactgccgtg cctgtgggat gtatctgaag
     2101 cacctgaaca gacaggtaga ggccatggag aagctcatca atctaactga catcctcaag
     2161 caggagaaga aggatgagac acagaaggta cagatgaagt tcttggttga acagatgaga
     2221 cagccagatt tcatggatgc tttgcagggt tttctgtccc ctctaaatcc tgctcatcaa
     2281 ctaggaaacc tcaggcttga agagtgtcga attatgtcct ctgcaaaaag gccactgtgg
     2341 ttgaattggg agaacccaga catcatgtca gagctactgt ttcagaacaa tgagatcatc
     2401 tttaaaaatg gcgatgactt acggcaagac atgttaaccc ttcagatcat ccgaatcatg
     2461 gagaacatct ggcaaaacca aggccttgac cttcgcatgc taccttatgg ctgtctatcc
     2521 attggggact gtgtgggtct catcgaggtg gtgagaaact ctcacaccat catgcagatt
     2581 cagtgcaaag gaggcctgaa gggggcactg cagttcaaca gccacacgct gcatcagtgg
     2641 ctcaaggaca agaacaaggg cgagatatat gatgcagcca ttgacctgtt cactcggtcc
     2701 tgcgctgggt actgcgtggc aacctttatc ttgggaattg gagaccggca caacagcaac
     2761 atcatggtga aagatgacgg acagctgttt catatagatt ttgggcactt tttggatcac
     2821 aagaagaaaa aatttggcta taaacgggaa cgtgtgccgt ttgttttgac gcaggatttc
     2881 ttaatagtga ttagtaaagg agcacaagag tacacaaaga ccagagagtt tgagaggttt
     2941 caggagatgt gttacaaggc gtacctagca attcggcagc atgccaatct cttcatcaac
     3001 cttttctcca tgatgcttgg ctccggaatg ccagaactgc agtctttcga tgatattgca
     3061 tatattcgaa agactctagc cttagacaaa actgagcaag aggctctgga gtatttcaca
     3121 aagcaaatga atgacgcaca tcatggtggc tggacaacaa aaatggactg gatcttccac
     3181 accatcaagc agcatgcatt gaactgagat ggcagctggg gactgcgagc tggtgcctag
     3241 cttctccact gcatggcagt gagtggcagc aggcacacgg tggcatggga tggcacagtc
     3301 aggaacaaca tcaggactcg agcaagaaca taaacaacgt gctctataat tgaaacactg
     3361 tacacgcaaa cagggtttga tagcactaaa ctagttcatt tcaaaattca gctttagaat
     3421 aatgagcaat ttcatgttat gccttaagtc caaaaaaagg taaactttga agattgtttg
     3481 tatctttttt aaaaaaacaa aacaaaacaa aaatccccaa aatacataca aatgatggag
     3541 acgaaaagag aatgctgttg tttgtctgcc agtgttctgt ccaagatgtg gacacccaag
     3601 ctgactgctg taggcccgag taagctgaag cttatattaa gttacatgaa attgaagaag
     3661 aatgaaaatt ctgattttcc caatgctgtt cagacttatg attggaagtg ggtattttga
     3721 ctccctgctt aatgaggaac aacttttggg gtgaagggat ttgctttttg ctttgaacaa
     3781 acaacccttc gatggacttg gggtctcacc tacggttttg aaagcagtca cgaatgatac
     3841 ctgcagacag ctgtgttttt gttgggtttt gtttttttgt tgtttttctc tctggacagt
     3901 atttacaagg atctgactta tttcctaggg aaattctggg ctcgcgtcaa gtacagcagt
     3961 aaccacagag gagaggcagc agggagctcc tgttcctgac ttgtacagta ttcactttaa
     4021 gctgattgtt tctccttttg caattgaact gaatactttt ttttcatgca tgttttccag
     4081 aaaatagaag tgagtattaa tgttattaaa aagattattt ttttttatta aaggctattt
     4141 atattataga aactatcatt aatatatatt ctttatttac ataatctgtc ccatagtcat
     4201 gcattgtttt gcaccccaaa ttttttattg ttggtaacag catggttagg ttttctcggt
     4261 ctatagatga ggctcaggca ctattccatt tacccagata cccctgtatg actccttaag
     4321 gaacagattg acctgcacat gctctccttc ctctgctgtc cgttttttac acgctgccct
     4381 cattgcacac ccatctgtag ttgagatggc acaatcctat gagatcagtt actgaaatga
     4441 atgcaaagca gactatcatc ctgactccta agtccctctg ctgaggtcag tcatgaatga
     4501 ctttttacat tatatatgag agactggaaa ccatgaattt tttaccttca taagctgtgt
     4561 atccatatcc aatgataaaa gtaggacatt aacccatttt tactatcatg tcccatttcc
     4621 aagtggtgag gtctcactcc gacttcatga tcccgttcag tcacggagtg cgttggtgag
     4681 cattctgagg agcgtccatc ctagatgtag ggctgctgtt agtggtcact ctagacttct
     4741 actccgtgtt tgaatgattc atgtcctaga aaatagcttt agcagatact cagatgccac
     4801 accaaaaaga aaaaaaaaaa aagaaaaaaa aagaaaaaga aaaaaaagtg caataattta
     4861 ctgacagttc ctagcttagg cgttattgga gagcatcttc atgaagagcg cacgctgtac
     4921 actctagaaa acacaatcct tttaataaag cgctcaccgt gaggtcagac acatatatag
     4981 aagttttgaa tagtaaacag gggactctaa taaaaatact cttaatatct gcctatttta
     5041 gaacccttaa agggcataat tattgaagat ttaggtactt cactaaagca tgtatatatt
     5101 attgccaaca agaaaaacct gaagattagg ggaacttagt tctgtaaact gtcttggaat
     5161 agttaagcag aatttaaact ctgttttatg cagaaaacca gatagattct tttgcagata
     5221 tagaaaactt cctaacttat ttaaacttgg catttaacac tttgtgttac ttagatatgc
     5281 agttgctagg tactaacatc ccattcttct ctatatcagg gattattaca gtcaaactca
     5341 gtgacatggt acaaatctac aactttgatg gtggaaactg aggaattaca gagaactgtt
     5401 ttcccgagtg ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa gccgcgagcc tccttgcaca
     5461 aaattgatag gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtttaaca
     5521 ttagttcatt agttgccgca gtatcacctc ccagggtctc tgcacaatta aaacacagcc
     5581 acatagctta ttttgtcact tactaccaca ctgattttat ttgaaagaaa gtctatattc
     5641 tataaagggt attagtaaag aaaggagaac tggtgtattc aaaaaggcaa aagccaaact
     5701 tgaatgaagc tggggtgatc aatatctgtt tccagagagc aataatgtgc cactcactcc
     5761 caggtgcttc agtaccagaa agtcctgcag acctggtctg taggtgaccg accttgtcat
     5821 taaatattgt ataaatgact tgggctccat agttacacta agtcatgtgg ggatgcttca
     5881 tcagtgattt ttttctggaa ggaaagaaaa caaatctaaa gaaagaaact aaatacactg
     5941 tagcaagaag taactcttaa atcatgcttt aactttttac catattctca gctataaaac
     6001 aaaacaactt tagtgtgaag attttagact gctgttcatt tgaaatctgt tgtttttact
     6061 gtggagtttg atttgtttgt ttgtttgctt tttgtttgtt tttaaagatg tttctaatta
     6121 gattttctaa aaaaagaaga atggaatctg gttgctattt taaggtagaa cctgagactt
     6181 ttgtggttct tcatgtcctc tgtaaaacgt ggtgtcaaga gtcatcaact ctgaggttgt
     6241 cccttctgtt atgctttata ttactgccca tcaggacatg ggaacctggt gaatatatga
     6301 tgacctgtaa aatattttaa atgtgtaact ttttcaactg tgaaactgac tattggtttt
     6361 ttgatgaaaa cagctgctca taaagtattt tgtgtaaagt gtagttctta ttaatcag
//
