LOCUS       NP_596890               1068 aa            linear   ROD 03-APR-2024
DEFINITION  phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit
            alpha isoform [Rattus norvegicus].
ACCESSION   NP_596890 XP_001059350 XP_001063573 XP_574916
VERSION     NP_596890.2
DBSOURCE    REFSEQ: accession NM_133399.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1068)
  AUTHORS   Wang,S., Duan,J., Liao,J., Wang,Y., Xiao,X., Li,L., Liu,Y., Gu,H.,
            Yang,P., Fu,D., Du,J., Li,X. and Shao,M.
  TITLE     LncRNA H19 inhibits ER stress induced apoptosis and improves
            diabetic cardiomyopathy by regulating PI3K/AKT/mTOR axis
  JOURNAL   Aging (Albany NY) 14 (16), 6809-6828 (2022)
   PUBMED   36044268
REFERENCE   2  (residues 1 to 1068)
  AUTHORS   Gao,H., Du,W.Y., Lin,J., Han,S.L., Zhang,Y.J. and Sun,X.F.
  TITLE     Losartan Protects Podocytes against High Glucose-induced Injury by
            Inhibiting B7-1 Expression
  JOURNAL   Curr Med Sci 41 (3), 505-512 (2021)
   PUBMED   34129205
  REMARK    GeneRIF: Losartan Protects Podocytes against High Glucose-induced
            Injury by Inhibiting B7-1 Expression.
REFERENCE   3  (residues 1 to 1068)
  AUTHORS   Beca,F., Krings,G., Chen,Y.Y., Hosfield,E.M., Vohra,P.,
            Sibley,R.K., Troxell,M.L., West,R.B., Allison,K.H. and Bean,G.R.
  TITLE     Primary mammary angiosarcomas harbor frequent mutations in KDR and
            PIK3CA and show evidence of distinct pathogenesis
  JOURNAL   Mod Pathol 33 (8), 1518-1526 (2020)
   PUBMED   32123305
REFERENCE   4  (residues 1 to 1068)
  AUTHORS   Seo,A.N., Kang,B.W., Bae,H.I., Kwon,O.K., Park,K.B., Lee,S.S.,
            Chung,H.Y., Yu,W., Jeon,S.W., Kang,H. and Kim,J.G.
  TITLE     Exon 9 Mutation of PIK3CA Associated With Poor Survival in Patients
            With Epstein-Barr Virus-associated Gastric Cancer
  JOURNAL   Anticancer Res 39 (4), 2145-2154 (2019)
   PUBMED   30952761
REFERENCE   5  (residues 1 to 1068)
  AUTHORS   Zhou,H., Tan,S., Li,H. and Lin,X.
  TITLE     Expression and significance of EBV, ARID1A and PIK3CA in gastric
            carcinoma
  JOURNAL   Mol Med Rep 19 (3), 2125-2136 (2019)
   PUBMED   30747208
REFERENCE   6  (residues 1 to 1068)
  AUTHORS   Lu,W., Bhasin,M. and Tsirka,S.E.
  TITLE     Involvement of tissue plasminogen activator in onset and effector
            phases of experimental allergic encephalomyelitis
  JOURNAL   J Neurosci 22 (24), 10781-10789 (2002)
   PUBMED   12486171
REFERENCE   7  (residues 1 to 1068)
  AUTHORS   Hers,I., Bell,C.J., Poole,A.W., Jiang,D., Denton,R.M., Schaefer,E.
            and Tavare,J.M.
  TITLE     Reciprocal feedback regulation of insulin receptor and insulin
            receptor substrate tyrosine phosphorylation by phosphoinositide
            3-kinase in primary adipocytes
  JOURNAL   Biochem J 368 (Pt 3), 875-884 (2002)
   PUBMED   12220227
  REMARK    GeneRIF: PI 3-kinase feedback control of signalling by the insulin
            receptor and IRS proteins is multifaceted and reciprocal
REFERENCE   8  (residues 1 to 1068)
  AUTHORS   Cros,N., Tkatchenko,A.V., Pisani,D.F., Leclerc,L., Leger,J.J.,
            Marini,J.F. and Dechesne,C.A.
  TITLE     Analysis of altered gene expression in rat soleus muscle atrophied
            by disuse
  JOURNAL   J Cell Biochem 83 (3), 508-519 (2001)
   PUBMED   11596118
REFERENCE   9  (residues 1 to 1068)
  AUTHORS   Pons,S., Asano,T., Glasheen,E., Miralpeix,M., Zhang,Y.,
            Fisher,T.L., Myers,M.G. Jr., Sun,X.J. and White,M.F.
  TITLE     The structure and function of p55PIK reveal a new regulatory
            subunit for phosphatidylinositol 3-kinase
  JOURNAL   Mol Cell Biol 15 (8), 4453-4465 (1995)
   PUBMED   7542745
REFERENCE   10 (residues 1 to 1068)
  AUTHORS   Carpenter,C.L., Duckworth,B.C., Auger,K.R., Cohen,B.,
            Schaffhausen,B.S. and Cantley,L.C.
  TITLE     Purification and characterization of phosphoinositide 3-kinase from
            rat liver
  JOURNAL   J Biol Chem 265 (32), 19704-19711 (1990)
   PUBMED   2174051
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On or before May 22, 2010 this sequence version replaced
            XP_001059350.1, XP_001063573.2, NP_596890.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data from several strains because no single
            transcript was available for the full length of the gene. The
            extent of this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.1851971.1,
                                           SRR26360176.982690.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     Protein         1..1068
                     /product="phosphatidylinositol 4,5-bisphosphate 3-kinase
                     catalytic subunit alpha isoform"
                     /EC_number="2.7.1.137"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.1.153"
                     /note="phosphatidylinositol 3-kinase, catalytic, alpha
                     polypeptide; phosphatidylinositol 4,5-bisphosphate
                     3-kinase 110 kDa catalytic subunit alpha; PI3Kalpha;
                     p110alpha; PI3K-alpha; PI3-kinase subunit alpha;
                     ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit
                     p110-alpha; serine/threonine protein kinase PIK3CA;
                     phosphoinositide-3-kinase catalytic alpha polypeptide"
                     /calculated_mol_wt=124224
     Region          31..108
                     /region_name="PI3K_p85B"
                     /note="PI3-kinase family, p85-binding domain; smart00143"
                     /db_xref="CDD:197539"
     Region          173..292
                     /region_name="PI3K_rbd"
                     /note="PI3-kinase family, Ras-binding domain; smart00144"
                     /db_xref="CDD:197540"
     Region          325..484
                     /region_name="C2_PI3K_class_I_alpha"
                     /note="C2 domain present in class I alpha
                     phosphatidylinositol 3-kinases (PI3Ks); cd08398"
                     /db_xref="CDD:176043"
     Site            order(345..346,410,447..450,452,469)
                     /site_type="active"
                     /note="p110alpha-p85alpha complex [active]"
                     /db_xref="CDD:176043"
     Region          525..696
                     /region_name="PI3Ka_I"
                     /note="Phosphoinositide 3-kinase (PI3K) class I, accessory
                     domain; PIK domain is conserved in all PI3 and
                     PI4-kinases. Its role is unclear but it has been suggested
                     to be involved in substrate presentation. In general, PI3K
                     class I prefer phosphoinositol (4,5)...; cd00872"
                     /db_xref="CDD:238444"
     Region          695..1064
                     /region_name="PI3Kc_IA_alpha"
                     /note="Catalytic domain of Class IA Phosphoinositide
                     3-kinase alpha; cd05175"
                     /db_xref="CDD:270719"
     Region          771..777
                     /region_name="G-loop.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00269"
                     /note="propagated from UniProtKB/Swiss-Prot
                     (A0A0G2K344.1)"
     Site            order(772,774..776,778,780,800,802,805,836,848..851,856,
                     859,920,922,932..933)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270719"
     Site            889
                     /site_type="other"
                     /note="Ras binding site [polypeptide binding]"
                     /db_xref="CDD:270719"
     Region          912..920
                     /region_name="Catalytic loop.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00269"
                     /note="propagated from UniProtKB/Swiss-Prot
                     (A0A0G2K344.1)"
     Site            912..920
                     /site_type="active"
                     /note="catalytic loop [active]"
                     /db_xref="CDD:270719"
     Region          931..957
                     /region_name="Activation loop.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00269"
                     /note="propagated from UniProtKB/Swiss-Prot
                     (A0A0G2K344.1)"
     Site            933..957
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270719"
     Site            order(944..945,947,949,1017,1020,1029)
                     /site_type="other"
                     /note="regulatory subunit interface [polypeptide binding]"
                     /db_xref="CDD:270719"
     CDS             1..1068
                     /gene="Pik3ca"
                     /coded_by="NM_133399.4:1..3207"
                     /db_xref="GeneID:170911"
                     /db_xref="RGD:620916"
ORIGIN      
        1 mpprpssgel wgihlmppri lvecllpngm ivtleclrea tlvtikhelf kearkyplhq
       61 llqdessyif vsvtqeaere effdetrrlc dlrlfqpflk viepvgnree kilnreigfv
      121 igmpvcefdm vkdpevqdfr rnilnvckea vdlrdlnsph sramyvyppn vesspelpkh
      181 iynkldkgqi ivviwvivsp nndkqkytlk inhdcvpeqv iaeairkktr smllsseqlk
      241 lcvleyqgky ilkvcgcdey flekyplsqy kyirscimlg rmpnlmlmak eslysqlpid
      301 sftmpsysrr istatpymng etatkslwvi nsalrikilc atyvnvnird idkiyvrtgi
      361 yhggeplcdn vntqrvpcsn prwnewlnyd iyipdlpraa rlclsicsvk grkgakeehc
      421 plawgninlf dytdtlvsgk malnlwpvph gledllnpig vtgsnpnket pclelefdwf
      481 ssvvkfpdms vieehanwsv sreagfsysh tglsnrlard nelrendkeq lralctrdpl
      541 seiteqekdf lwshrhycvt ipeilpklll svkwnsrdev aqmyclvkdw ppikpeqame
      601 lldcnypdpm vrsfavrcle kyltddklsq yliqlvqvlk yeqyldnllv rfllkkaltn
      661 qrighfffwh lksemhnktv sqrfgllles ycracgmylk hlnrqveame klinltdilk
      721 qekkdetqkv qmkflveqmr qpdfmdalqg flsplnpahq lgnlrleecr imssakrplw
      781 lnwenpdims ellfqnneii fkngddlrqd mltlqiirim eniwqnqgld lrmlpygcls
      841 igdcvgliev vrnshtimqi qckgglkgal qfnshtlhqw lkdknkgeiy daaidlftrs
      901 cagycvatfi lgigdrhnsn imvkddgqlf hidfghfldh kkkkfgykre rvpfvltqdf
      961 liviskgaqe ytktreferf qemcykayla irqhanlfin lfsmmlgsgm pelqsfddia
     1021 yirktlaldk teqealeyft kqmndahhgg wttkmdwifh tikqhaln
//
