LOCUS       NM_001108500            8316 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus phosphatidylinositol-4-phosphate 3-kinase,
            catalytic subunit type 2 alpha (Pik3c2a), mRNA.
ACCESSION   NM_001108500 XM_001075241 XM_341911
VERSION     NM_001108500.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Lu H, Chen Y, Chen Y, Huang L and Chen L.
  TITLE     C/EBPalpha-Mediated Transcriptional Activation of PIK3C2A Regulates
            Autophagy, Matrix Metalloproteinase Expression, and Phenotypic of
            Vascular Smooth Muscle Cells in Aortic Dissection
  JOURNAL   J Immunol Res 2022, 7465353 (2022)
   PUBMED   36132983
  REMARK    GeneRIF: C/EBPalpha-Mediated Transcriptional Activation of PIK3C2A
            Regulates Autophagy, Matrix Metalloproteinase Expression, and
            Phenotypic of Vascular Smooth Muscle Cells in Aortic Dissection.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Xiaoyu L, Wei Z, Ming Z and Guowei J.
  TITLE     Anti-apoptotic Effect of MiR-223-3p Suppressing PIK3C2A in
            Cardiomyocytes from Myocardial Infarction Rat Through Regulating
            PI3K/Akt Signaling Pathway
  JOURNAL   Cardiovasc Toxicol 21 (8), 669-682 (2021)
   PUBMED   33999393
  REMARK    GeneRIF: Anti-apoptotic Effect of MiR-223-3p Suppressing PIK3C2A in
            Cardiomyocytes from Myocardial Infarction Rat Through Regulating
            PI3K/Akt Signaling Pathway.
REFERENCE   3  (bases 1 to 8316)
  AUTHORS   Merrill NM, Schipper JL, Karnes JB, Kauffman AL, Martin KR and
            MacKeigan JP.
  TITLE     PI3K-C2alpha knockdown decreases autophagy and maturation of
            endocytic vesicles
  JOURNAL   PLoS One 12 (9), e0184909 (2017)
   PUBMED   28910396
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8316)
  AUTHORS   Tang Y, Zhang Y, Chen Y, Xiang Y and Xie Y.
  TITLE     Role of the microRNA, miR-206, and its target PIK3C2alpha in
            endothelial progenitor cell function - potential link with coronary
            artery disease
  JOURNAL   FEBS J 282 (19), 3758-3772 (2015)
   PUBMED   26175229
REFERENCE   5  (bases 1 to 8316)
  AUTHORS   Nigorikawa K, Hazeki K, Guo Y and Hazeki O.
  TITLE     Involvement of class II phosphoinositide 3-kinase alpha-isoform in
            antigen-induced degranulation in RBL-2H3 cells
  JOURNAL   PLoS One 9 (10), e111698 (2014)
   PUBMED   25357130
  REMARK    GeneRIF: PI3K-C2alpha and its product PtdIns(3,4)P2 may play roles
            in the secretory process.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8316)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 8316)
  AUTHORS   Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta
            R, Wang NS and Knepper MA.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 8316)
  AUTHORS   Yoshioka K, Sugimoto N, Takuwa N and Takuwa Y.
  TITLE     Essential role for class II phosphoinositide 3-kinase alpha-isoform
            in Ca2+-induced, Rho- and Rho kinase-dependent regulation of myosin
            phosphatase and contraction in isolated vascular smooth muscle
            cells
  JOURNAL   Mol Pharmacol 71 (3), 912-920 (2007)
   PUBMED   17179444
  REMARK    GeneRIF: PI3K-C2alpha is necessary for Ca2+-induced Rho- and Rho
            kinase-dependent negative regulation of MLCP and consequently MLC
            phosphorylation and contraction.
REFERENCE   9  (bases 1 to 8316)
  AUTHORS   Wang Y, Yoshioka K, Azam MA, Takuwa N, Sakurada S, Kayaba Y,
            Sugimoto N, Inoki I, Kimura T, Kuwaki T and Takuwa Y.
  TITLE     Class II phosphoinositide 3-kinase alpha-isoform regulates Rho,
            myosin phosphatase and contraction in vascular smooth muscle
  JOURNAL   Biochem J 394 (Pt 3), 581-592 (2006)
   PUBMED   16336212
  REMARK    GeneRIF: The role of PI3K-C2alpha in Ca2+-dependent Rho-mediated
            negative control of myosin phosphatase and thus vascular smooth
            muscle contraction is reported.
REFERENCE   10 (bases 1 to 8316)
  AUTHORS   Virbasius JV, Guilherme A and Czech MP.
  TITLE     Mouse p170 is a novel phosphatidylinositol 3-kinase containing a C2
            domain
  JOURNAL   J Biol Chem 271 (23), 13304-13307 (1996)
   PUBMED   8663140
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473956.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_341911.3, XM_001075241.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q35"
     gene            1..8316
                     /gene="Pik3c2a"
                     /note="phosphatidylinositol-4-phosphate 3-kinase,
                     catalytic subunit type 2 alpha"
                     /db_xref="GeneID:361632"
                     /db_xref="RGD:1310389"
     exon            1..146
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            147..232
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            233..1361
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..5354
                     /gene="Pik3c2a"
                     /EC_number="2.7.1.154"
                     /note="phosphatidylinositol 3-kinase, C2 domain
                     containing, alpha polypeptide; phosphoinositide-3-kinase,
                     class 2, alpha polypeptide"
                     /codon_start=1
                     /product="phosphatidylinositol 4-phosphate 3-kinase C2
                     domain-containing subunit alpha"
                     /protein_id="NP_001101970.1"
                     /db_xref="GeneID:361632"
                     /db_xref="RGD:1310389"
                     /translation="MAQISNNSEFKQCSSSHPEPIRTKDVDKAEALQMEAEALAKLQK
                     DRHMTDSPRGFELSNSTRQRTQGFNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEK
                     ILLDDNFETRKTPASPVTPVLSPSFSAQLYLRPAVQRSQWPPGLCGPSTYTLPSIYPS
                     AYSKQATFQNGFSPRMPTFPSTESIYLRLPGQSPYFSYPLTPATPFHPQGSLPVYRPL
                     VSPDMAKLFDKIASTSEFLKNGKARTDLEIANSKASVCNLQISPKSEDINKFDWLDLD
                     PLSKPKIDCVEVLEHEEEKKNPVLLAEDPWDAVLLEERSPTSRHLERKVNGKSLSGAT
                     VTRSQSLIVRTTQVTKAQSQVSQKDPNGTSSLATGSSLLQEFEVQNEEVAAFCQSIMK
                     LKTKFPYTDHCTNPGYLLSPVTVQRNMCGENASVKVSIEIEGFQQPVTFTCDVSSTVD
                     IIIMQALCWVHDDLNQVDVGSYILKVCGQEEVLQNNHCLGSHEHIQNCRKWDTEIKLQ
                     LLTFSAMCQNLARTAEDDEAPVDLNKYLYQIEKPYKEVMTRHPVEELLDSYHYQVELA
                     LQTENQHRAIDQVIKAVRKICSALDGVETPAVTEAVKKLKRAVNLPRNKSADVTSLSG
                     GDTSKNSTRGSLNLENPVQVSMDHLTAAIYDLLRLHTNSCKSSTVCPQGSRNIKEAWT
                     ATEQLQFTVYAAHGISSNWVSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLI
                     KWDELIIFPIQISQLPLESVLHLTLFGVLNQSSGSSPDSNKQRKGPEALGKVSLTLFD
                     FKRFLTCGTKLLYLWTSSHTNSIPGAIPQKSYVMERIVLQVDFPSPAFDIIYTSPQID
                     RNIIQQYKLEALENEIKGKLLDIVHRDSSFGLSKEDKAFLWEKRYYCLKHPNCLPKIL
                     ASAPNWKWANLAKTYSLLHQWPPLCPLAALELLDAKFADQEVRSLAVSWIEAISDDEL
                     TDLLPQFVQALKYEIYLNSSLVRFLLSRALGNIQIAHSLYWLLKDALHDTRFGSRYEH
                     VLGALLSVGGKGLREELSKQMKLVQLLGGVAEKVRQASGSARQVVLQKSMERVQSFFL
                     RNKCRVPLKPSLVAKELNIKSCSFFSSNAMPLKVTMVNADPLGEEINVMFKVGEDLRQ
                     DMLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDQGMVELVPASDTLRKIQVEYGVT
                     GSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRST
                     GHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQA
                     YNLIRKQTNLFLNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIES
                     SLGSIATKFNFFIHNLAQLRFSGLPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKK
                     YNPDKHYIYVVRILREGHLEPSFVFRTFDEFQELHNKLSIIFPLWKLPGFPNRMVLGR
                     THIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKAEGIARSTGTSP
                     FSPTLGQIGGAVKLSVSYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDTHKTSK
                     RKTKISRKTRNPTFNEMLVYSGYSRETLRQRELQLSVLSAESLRENFFLGGITLPLKD
                     FNLSKETVKWYQLTAATYL"
     exon            1362..1465
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1623
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1744
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1856
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..1936
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2000
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2144
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2190
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2401
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2524
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2692
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2804
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2950
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3139
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3233
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3328
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3329..3509
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3510..3614
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3615..3707
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3708..3837
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3838..3974
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3975..4144
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4145..4301
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4302..4411
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4412..4619
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4620..4744
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4745..4862
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4863..4935
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4936..5045
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            5046..5171
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
     exon            5172..8316
                     /gene="Pik3c2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtacagtgct cggctggccg cggagtcagt cgaagctctc ctcagcggcc ggctgagcca
       61 gctgaggcgg gagaaaaaca tggctcggac ctttgagggc gcgaaggctt gggttgcggt
      121 ggagactggg gctcccgtag cgtgagccag tgggtgtttt gaattgagtg acacgaagga
      181 agcatccctg tttgaacaag attacttaag aagtattccc aaatcttacc aggtcctggt
      241 attttggaag ccacaagaaa aaagattaag agatttggtc tcttgtggac atggctcaga
      301 tttccaacaa cagtgaattt aaacagtgtt catcttcaca tccagaacca ataagaacca
      361 aagatgtgga caaagcagaa gcattacaga tggaagcaga agccttagca aaattgcaga
      421 aggatagaca catgactgac agtccaagag gctttgagct gtctaacagc actagacaaa
      481 gaacacaggg ttttaacaaa caggattatg atctcatggt gtttcctgag tcggattccc
      541 aaaaaagagc attagatatt gacgtagaaa agcttaccca agctgaactt gaaaagatat
      601 tgctggatga caattttgaa actagaaaaa ctcctgcatc gccagttact cctgttctga
      661 gcccttcatt ttcagcacag ctatatctta gacctgctgt tcaaagaagc cagtggcccc
      721 ctggattatg cgggccttcc acatacactt taccttctat ttatccttca gcatacagta
      781 aacaggccac attccagaat ggcttcagtc caaggatgcc tacttttcca tctacagagt
      841 ctatatattt aagacttcca ggacagtctc catatttttc atatcctttg acacctgcca
      901 caccatttca tccacaagga agtttaccag tctatcggcc actagtcagt cctgacatgg
      961 caaaactatt tgacaaaata gcaagtacct cagaattttt aaaaaatggg aaagcaagga
     1021 ctgatttgga gatagcaaac tcgaaagctt cagtctgcaa tctacaaata tctccaaagt
     1081 ctgaagacat caataaattt gattggttgg acttggatcc tctgagtaag cctaagatag
     1141 actgtgtgga agtgttagaa catgaagaag agaagaagaa tccagttttg ctagcagagg
     1201 atccttggga tgctgttctt cttgaagaga gatcaccaac aagtcgtcac ctagaaagga
     1261 aggtgaatgg aaaatccctt tctggggcga ctgtaacaag aagccagtct ttaatcgttc
     1321 ggacaactca agttacaaaa gcccagagcc aggtatctca gaaagaccca aatgggacca
     1381 gtagtttggc aactggaagt tctcttctac aagaatttga agtacagaat gaggaggtgg
     1441 cagctttttg tcaatccatt atgaaattaa agaccaaatt tccatatacc gatcactgta
     1501 caaatccagg ctatttgtta agtccagtca cagtgcaaag aaacatgtgt ggggaaaatg
     1561 ccagtgtgaa ggtctccatt gaaattgaag ggtttcaaca accagttact tttacatgtg
     1621 atgtgagttc tactgtagac ataattataa tgcaagccct ttgctgggta catgatgact
     1681 tgaatcaagt ggatgttggc agctacattc tgaaagtttg tggtcaagag gaggttctac
     1741 agaataatca ttgccttgga agtcacgaac atattcaaaa ttgtcgaaaa tgggacacag
     1801 agattaaatt acaactcttg accttcagtg caatgtgcca gaatctggct cgaacagcag
     1861 aagatgatga agcacctgtg gatttaaaca agtacttgta tcaaatagaa aaaccttata
     1921 aagaagtcat gacaagacac cctgttgaag aactcttaga ttcctatcac taccaagtag
     1981 aactggctct acaaactgaa aaccagcacc gagctattga tcaagtgatt aaagcagtaa
     2041 gaaaaatctg tagtgcttta gatggggttg agacccccgc cgtcacagaa gcagtgaaga
     2101 agctaaagcg agcagttaac cttccaagga acaaaagtgc tgatgtgact tcattatctg
     2161 gaggagatac tagcaagaac tcaactaggg gctcactgaa tcttgaaaat cctgttcaag
     2221 taagcatgga tcacttaacg gcagcaattt atgatcttct caggcttcat acaaattctt
     2281 gtaagagttc tacagtctgt ccccaaggta gcaggaacat caaggaagca tggactgcaa
     2341 cagagcagct ccagttcacc gtctatgccg cacacggaat ttccagtaac tgggtatcca
     2401 attacgaaaa atactacttg atatgttccc tgtctcacaa tgggaaggat ctttttaagc
     2461 ctattcagtc aaagaaggtt ggcacataca agaatttctt ctatcttatc aaatgggatg
     2521 aactaatcat tttccctatc cagatatcgc agttgccttt agagtcagtt cttcatctca
     2581 ctttgtttgg agttttaaat cagagcagtg gaagttcccc tgattctaat aaacagagaa
     2641 aggggccaga agctctgggc aaagtctctt taactctatt tgactttaaa cggtttttaa
     2701 catgtggaac taaacttctc tacctttgga cttcatcaca tacaaattct attcctggag
     2761 caatccccca aaaaagctat gtcatggaaa gaattgtgct acaggttgat tttccttctc
     2821 ctgcatttga cattatttat acatctcctc aaattgatag aaacattata cagcaataca
     2881 agctggaagc actggagaat gaaataaagg gaaaacttct ggatattgtt cacagagatt
     2941 catcatttgg actttctaaa gaagataagg cctttttgtg ggaaaaacgc tattattgcc
     3001 taaaacatcc aaattgtctt ccgaagatac tagcaagtgc tccaaactgg aagtgggcta
     3061 atcttgccaa aacttactct ttgctccacc agtggccgcc attgtgtcca ttagctgcat
     3121 tggagctcct tgatgcaaaa tttgctgatc aggaagtgcg atcgctagct gtgagctgga
     3181 ttgaggccat tagtgatgat gagctaacag atcttctccc acagtttgta caggctttga
     3241 aatatgaaat ttatttgaat agttcactag tgcgcttcct tctgtccagg gcactgggaa
     3301 acatccagat agcacacagt ttgtattggc ttctcaagga tgctttgcac gatacacggt
     3361 ttggaagcag atatgaacat gtgttgggtg ctctcctctc tgtaggagga aaaggactca
     3421 gagaagagct ttctaagcag atgaaacttg tacagctttt aggaggagtg gcagaaaaag
     3481 taaggcaggc tagtggatca gccagacagg tggtcctcca gaagagtatg gaacgagtac
     3541 agtccttttt tctgagaaat aaatgccggg ttcctctcaa accaagtcta gtagcgaaag
     3601 aattaaatat taagtcatgc tcattcttca gttcgaatgc catgcctctg aaagtcacaa
     3661 tggtgaatgc tgatcctctg ggagaagaaa ttaatgtcat gtttaaggtt ggtgaagatc
     3721 ttcggcaaga tatgttagct ttacagatga taaagattat ggataagatc tggcttaaag
     3781 agggcctgga tctgaggatg gtgatattca aatgcctgtc gactggccga gatcaaggca
     3841 tggtggagct agttcctgcc tcagacactc tcaggaaaat ccaagtggaa tatggtgtga
     3901 caggatcctt taaagataaa ccacttgctg aatggctaag gaaatacaac ccttctgaag
     3961 aagaatatga aaaggcttct gagaacttta tctactcttg tgctgggtgc tgtgtagcca
     4021 cttatgtttt aggcatttgt gatcggcaca atgacaatat aatgcttcga agcacaggac
     4081 acatgttcca cattgacttt ggaaagtttt tgggccatgc acagatgttt ggtagcttca
     4141 aaagggaccg cgctcctttc gtgcttacct ctgacatggc ctatgtcatt aatggaggtg
     4201 aaaagcccac cattcgtttc cagttgtttg tggacctctg ctgtcaagcc tacaacttga
     4261 taagaaagca aacaaacctc tttcttaacc ttctctcact gatgattcct tcaggattgc
     4321 cagaacttac aagtattcag gatttgaaat atgttagaga tgcacttcag ccccaaacta
     4381 cagatgctga agctactatt ttctttacta ggctgattga gtcaagtttg ggaagcattg
     4441 ccacaaagtt taatttcttc attcataacc ttgctcagct acgtttttct ggccttcctt
     4501 ctaatgatga gcccatcctt tcattttcac caaaaacata ctcctttaga caagacggcc
     4561 ggatcaaaga agtctctgtt ttcacatatc ataagaaata caacccagat aaacactata
     4621 tttatgtggt tcgaattctg agggaaggac accttgaacc atcatttgta ttccggacat
     4681 ttgatgagtt tcaggaactg cacaataagc tcagcattat tttcccactt tggaagttac
     4741 ctggctttcc taataggatg gttctaggaa gaacacacat caaagatgta gcagccaaga
     4801 ggaaaattga attaaacagt tatttacaga gtttgatgaa tgcatcaaca gatgtagcag
     4861 agtgtgacct tgtttgtact tttttccacc ccttacttcg tgatgaaaaa gctgaaggaa
     4921 tagctaggtc tacaggtaca agtcccttca gccctacttt gggccaaata ggaggagcag
     4981 tgaagttatc ggtttcttac cgaaatggca ccctcttcat catggtgatg cacatcaaag
     5041 atctggtgac tgaagatggg gctgacccaa atccatacgt caaaacatac ctgcttccag
     5101 atacccacaa aacgtcaaaa cgtaaaacca aaatttcacg taaaactagg aacccaacat
     5161 tcaatgaaat gcttgtatat agtggataca gcagagaaac tctgagacag agagaacttc
     5221 aactgagtgt gctcagtgca gaatctctgc gggagaattt cttcctgggt ggaataaccc
     5281 tgccactgaa agatttcaac ttgagcaaag agacagttaa gtggtatcag ctgactgcgg
     5341 caacatacct ataaacctcc aacttccgag ctttggaagc aaggaattat aaatgtatgc
     5401 acatgtgtgt atgtgttctc atacacacgc cttgggaact ttatacaatt tcatacttgg
     5461 ttagaaatta taaacttaac atgttggacc aaccgtcagc cacataacta acttttatag
     5521 atttttgaga agccattgct gtttcagagt cattttgttg actcctacca attccaaact
     5581 taatacgtac taagtcctaa aaaagacttt gacataatac tgtgttattt cggccacttt
     5641 gtctgtattc ttcctataca gacacagcaa attgtttttt gttgtacacc tcacagcctt
     5701 tacctctgtg tgtgttcaca gacaccaaag gaaagatgcg gctgctactc aacaggctga
     5761 aaagcaaagc acaaacagta gtcaggatgc taaggactac taagtagtct acagaagtag
     5821 gaaaaaatac tatttttatt cattgttaag ccttttcaga gtaagtgcca atcactgaag
     5881 ttgtaaataa tggtgcctta ctgaactctg tgcttcactg gcacttcatt tctgttaaag
     5941 agcaagtcgt tttcactcat tttcacctta ctgaggagga caaaatatca tgtacattta
     6001 ctaatggttt cctcacaaaa atactatttt ctctacttct gattgtgact ttattttcac
     6061 tattgagcta tttgacttaa tgttaattgc ccagtaattg aatgagattc attgatactg
     6121 ttgtattcta aattctatca ttccattatt atttgcaagg gtgtgaactg aaattggcat
     6181 ttcacttcca tgtatctaaa ctttattccc tcattaaaca aatggaaatt gttcagcctt
     6241 atgcataata accattttta tttggtaaga tgcccgggcg gcataaggag ttagggtcct
     6301 gactgaactt taatttagac atttatggaa ggtgttgtgt aaactgtcct gtatttgtag
     6361 acattgtaca ttcatgtgaa agaactattt tctgtactac aatgaaactc agaatttcaa
     6421 gagaaaagtt tgaagttatt agggagtagc cattaagaat aacattaatc tttatttccc
     6481 ctttcaaata taattttgtt tagttttggt aaacatgtat atataataaa tttcttggtt
     6541 agaagttcaa atatttttag gatggtaata gagatgtagc agatttggta gttaagagaa
     6601 aactgacaga ctatttaatg aagggtacca agagaggaca atctgattca gttttccaga
     6661 ataaaatttt tatgaagtat acaaagtgat taacagaagt gatgcttaaa agagttaagg
     6721 gtgttaccat tggaatacat ttttaaaaat caaaacttga agtatttggt gcctaacact
     6781 atggtccatt tgtagggaaa tgagtatgag tatgtgaatt ttttatatta aagtgtgttg
     6841 tctacttgat aattaaaatg aagatcaaaa caccattgtt tgctttttcc acaattaaca
     6901 tttccttaaa ttgttttgca gatgtaaata gcattagaat ttttttttct tttgagagag
     6961 acctgattcc attccatgag tcacatagct acttaaaaaa aaaaaaaaac aaaaacgctg
     7021 tttgtaaaag gtggtcattg actggactca tggacctttt tgtgacctgt tactgtcaca
     7081 aaatattaag atattagcag aaacagaatt agaaaatgta atgtcacttt tcagtgacaa
     7141 gaaatctgat taaacggggt tggggattta gctcagtggt aggtcctggg ttcggtcccc
     7201 agctccgaaa aaaaaaaaaa agaaatctga ttaatatgta aaagtatata ttataattaa
     7261 tactgtatag ttaacaagaa ttcaaaaggt attattgtct aatttaactc aatatgtgcc
     7321 ttctttggtc attagactac atatatctta atataagtta tttctttgta aaatgctaat
     7381 cttcagccca taccaaaaaa acaatgatag ggaggagaaa gtagcctctt tgcactacaa
     7441 ctgcagccaa ttataatcag ctggctttct aaaagcttct taacagttat cttgaaataa
     7501 atctgaaaaa caaaactcta aaaaaacagg tcaaaacact aatttttatg tatatcatat
     7561 ttataactac cttcatcaag taaaaataat tctgctacat acaagtcctg tgccagaatt
     7621 ctttgttctt gccagtgctc taggacctga agataatggt tcatcccaag aacctgtttt
     7681 ctttgaggaa tccaatgaga attggcacca gtgcttttta ttactgtgtt tatgtgaatg
     7741 agttgttaaa gcatgtgtat ctttttaagt actttttttt caaattaaag ttgtatctaa
     7801 agttcagaat cttgccttaa attgtttaat tctttctaag actcatgaat tgtaacattt
     7861 aaaaaattca cttttgataa ctcagatttt aaaaacactg aagccctgct atcatgatac
     7921 acatttactt acatgctgtc cactagcaat gcgaatatgg atgtggttac tagtgtcacc
     7981 tgtaagaaca aaatgcttta attatatcag catagtgaga taagatgtat aatattttta
     8041 ttaatacttg gaatatattc agtgaattga atgtgacttc ataactatac tacaattata
     8101 ttaaaatatt tctgaaataa aggaactcag ctgtcatttc cttccttggt tcaatacttt
     8161 ggtttcaaac tttaatatgg ttattgcttc ttacccagga gtttgtaagg tttagtaatt
     8221 ctaagttcga atgtgattct ttgaaggtct ggatattgtc tgctacagcc tatcacttcc
     8281 tgagatgtga attctgtaga tatggttcct ggcctg
//
