LOCUS       NM_001100651             988 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis,
            class X (Pigx), mRNA.
ACCESSION   NM_001100651 XM_001073238 XM_213602
VERSION     NM_001100651.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Ashida,H., Hong,Y., Murakami,Y., Shishioh,N., Sugimoto,N.,
            Kim,Y.U., Maeda,Y. and Kinoshita,T.
  TITLE     Mammalian PIG-X and yeast Pbn1p are the essential components of
            glycosylphosphatidylinositol-mannosyltransferase I
  JOURNAL   Mol Biol Cell 16 (3), 1439-1448 (2005)
   PUBMED   15635094
  REMARK    GeneRIF: A non-AUG translation initiation site (CUG) is used in rat
            Pigx
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB177393.1 and DY311520.1.
            
            On or before Dec 1, 2007 this sequence version replaced
            XM_001073238.1, XM_213602.4.
            
            Summary: This gene encodes a type I transmembrane protein in the
            endoplasmic reticulum (ER). The protein is an essential component
            of glycosylphosphatidylinositol-mannosyltransferase I, which
            transfers the first of the four mannoses in the GPI-anchor
            precursors during GPI-anchor biosynthesis. The protein is
            translated from a non-AUG translation initiation site. [provided by
            RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB177393.1, SRR8487227.288311.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 15635094
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AB177393.1         1-49
            50-499              DY311520.1         48-497
            500-988             AB177393.1         500-988
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q22"
     gene            1..988
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class X"
                     /db_xref="GeneID:288041"
                     /db_xref="RGD:1307289"
     exon            1..252
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..917
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /note="non-AUG (CUG) translation initiation codon;
                     GPI-mannosyltransferase subunit"
                     /codon_start=1
                     /product="phosphatidylinositol-glycan biosynthesis class X
                     protein precursor"
                     /protein_id="NP_001094121.1"
                     /db_xref="GeneID:288041"
                     /db_xref="RGD:1307289"
                     /translation="MAASALAWLLLWAAGLVGRLAADISDARFSDGVRATCSEIILRQ
                     EFLKDGFHRDLLIKVKFGESIEDLQTCRLLIKHYIPTGLFVDPYELASLRERNITEAV
                     MVSESFNLEAPSYLSTESAVLIYARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTLIVV
                     NNPDLLMHCDQEFPILKCWAQSEVAAPCSLKSEEICQWKNMQYKSILKNLTVQVPVGL
                     TIHTSLVCSVTLLITVLCSTLILLAVFKYGHFSL"
     exon            253..316
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /inference="alignment:Splign:2.1.0"
     exon            317..458
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /inference="alignment:Splign:2.1.0"
     exon            459..672
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /inference="alignment:Splign:2.1.0"
     exon            673..773
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /inference="alignment:Splign:2.1.0"
     exon            774..970
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
                     /inference="alignment:Splign:2.1.0"
     regulatory      946..951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
     polyA_site      970
                     /gene="Pigx"
                     /gene_synonym="RGD1307289"
ORIGIN      
        1 ctcaggaacc tggagttaca gggggtccta gggtgggagc gcaaggagcg ttctgtccgg
       61 gtcaccgtgg aaggtggggg tacgctctgc gtgctcaggt tcctcccgtg accgcagctt
      121 gctccgcgcg cccctctcgg gcgtcgggct ccggcgtcct ggctgccagc gcccttgcct
      181 ggctgctcct ctgggcagct gggctggtcg gcaggctcgc cgcagacatc agcgacgccc
      241 gcttcagcga tggtgtaagg gccacttgtt ctgaaattat tttgaggcaa gaatttttga
      301 aagatggttt ccacagagac cttttaataa aagtgaaatt tggggaaagc atcgaggacc
      361 tacagacctg ccggctccta atcaaacact acatcccaac aggactcttt gtggacccgt
      421 acgagttggc ttcattacgg gaaagaaaca taacagaggc agtgatggta tcagagagtt
      481 ttaatctaga agcccccagc tatttatcca cagagtctgc ggtcctcatt tatgcccggc
      541 aggacgcaca gtgcatcgac tgcttccagg ccttcctacc tgtgcactat cgatatcacc
      601 gtccacataa gaaggatgga gacaccctca ttgtggtcaa caaccctgac ttactgatgc
      661 actgtgacca agagttccca attttgaaat gctgggctca gtcagaagta gcagctcctt
      721 gttctttgaa gagtgaggag atctgccagt ggaaaaacat gcagtacaaa tcaatactta
      781 agaatttgac agtgcaggtg ccagtgggac tgactataca cacctcctta gtgtgctccg
      841 tgacactgct cattacggtt ctgtgttcta ctctgatcct tttagctgtt ttcaaatacg
      901 gccatttttc cctgtaagtt ttatgcagtt aagtgttttc tacctaataa atgagacata
      961 ttcattgtga aaaaaaaaaa aaaaaaaa
//
