LOCUS       NM_001011953            1557 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis,
            class K (Pigk), mRNA.
ACCESSION   NM_001011953 XM_215723
VERSION     NM_001011953.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Ohishi K, Inoue N and Kinoshita T.
  TITLE     PIG-S and PIG-T, essential for GPI anchor attachment to proteins,
            form a complex with GAA1 and GPI8
  JOURNAL   EMBO J 20 (15), 4088-4098 (2001)
   PUBMED   11483512
REFERENCE   3  (bases 1 to 1557)
  AUTHORS   Ohishi K, Inoue N, Maeda Y, Takeda J, Riezman H and Kinoshita T.
  TITLE     Gaa1p and gpi8p are components of a glycosylphosphatidylinositol
            (GPI) transamidase that mediates attachment of GPI to proteins
  JOURNAL   Mol Biol Cell 11 (5), 1523-1533 (2000)
   PUBMED   10793132
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC083636.1.
            
            On Feb 11, 2005 this sequence version replaced XM_215723.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083636.1, SRR8487231.186329.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q45"
     gene            1..1557
                     /gene="Pigk"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class K"
                     /db_xref="GeneID:295543"
                     /db_xref="RGD:1306753"
     exon            1..103
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1198
                     /gene="Pigk"
                     /EC_number="3.-.-.-"
                     /note="phosphatidylinositol glycan, class K; GPI-anchor
                     transamidase"
                     /codon_start=1
                     /product="GPI-anchor transamidase precursor"
                     /protein_id="NP_001011953.1"
                     /db_xref="GeneID:295543"
                     /db_xref="RGD:1306753"
                     /translation="MASPCFFTLPVAVLATLAFLSLGSSAASHIEDQAEQFFRSGHTN
                     NWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNARNPKPATV
                     FSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRVPPSTPRSKRLLSDDRSNILIY
                     MTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSP
                     NIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCP
                     KSLCVSTPGHRTDLFQRDPKKVLITDFFGSVRKVEITTETISLQWDSQVVDSSSAEDG
                     MAEEPTEPLKFAEQLPVAQIIHQKPKLKDWHPPGGFILGLWALIIMVFFKTYGIKHMK
                     FIF"
     exon            104..157
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            158..249
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            250..385
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            386..497
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            498..594
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            595..712
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            713..823
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            824..996
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1081
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1532
                     /gene="Pigk"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccggcaaac atggcgtccc cctgcttctt taccctgcca gtggctgtct tggccacctt
       61 ggcgttcttg tctctcggca gctccgccgc tagtcacatc gaggatcaag cagaacaatt
      121 ctttagaagt ggacatacaa ataactgggc tgttttggta tgcacatccc gattctggtt
      181 taattaccga catgttgcaa atactctctc tgtttacaga agcgtcaaga ggctaggtat
      241 tccagacagt cacattgtcc ttatgcttgc tgatgacatg gcgtgcaatg ctcggaaccc
      301 caagccagcc acagtgttca gccacaagaa catggagctc aatgtgtatg gggacgacgt
      361 ggaagtggac tacagaagct atgaggtgac cgtggagaac tttttacggg tattaactgg
      421 gagggttcca cccagcaccc ctcgttcaaa gcgtcttctc tccgacgaca gaagcaatat
      481 cctcatttat atgacaggtc atggaggaaa cggttttttg aaattccaag attctgaaga
      541 aatcactaac atagaacttg cagatgcgtt tgaacagatg tggcagaaaa gacgctacaa
      601 cgagctgctg ttcattatcg acacttgcca gggcgcgtcc atgtatgagc ggttttactc
      661 tcctaacatc atggccttgg cgagtagcca agtgggcgag gattccctgt cgcaccagcc
      721 cgaccccgca attggcgtcc accttatgga caggtacaca ttctatgtct tggaattttt
      781 ggaagagatt aatccagcta gccaaactaa catgaatgac ctttttcaag tgtgtcccaa
      841 aagcctctgt gtgtctaccc ctggacatcg tactgacctt ttccagcgag atcctaaaaa
      901 ggtcctgata accgatttct tcggaagcgt gcggaaggtg gaaatcacga cagagacgat
      961 cagtttgcag tgggattcac aagtcgtgga cagcagttct gccgaggacg gaatggcgga
     1021 ggagcccacg gaacctctca agttcgctga gcagctcccg gtggctcaga taatacacca
     1081 gaagccgaag ctgaaggact ggcaccctcc cggaggcttc atcctggggc tgtgggcgct
     1141 catcatcatg gtcttcttta agacctacgg catcaagcac atgaagttca ttttttagtc
     1201 tcaaggatgt ttggagaaga atgaatggaa gactgcagcc gtggataaaa cttagatttt
     1261 tatatgtttt taaagatgtt acttttatgt aaatttggga agaaaattag gaaagttgaa
     1321 ttttaatgaa cttttgactc tttttaactt agtattgtta acacacttgt ttattggcca
     1381 cagaatgtgt cttccattgt gtttgtgtgt gtgtgagtta tagaaaccag aggcaatgtc
     1441 actggagcat atcgcttaat tcttttatcc tcctctcatt gtaaaagtaa gagttatgac
     1501 gggggaactc ccactaaagg aggttacttt aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
