LOCUS       NM_001401036            2560 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus phosphatidylinositol glycan anchor biosynthesis,
            class A (Piga), transcript variant 1, mRNA.
ACCESSION   NM_001401036 XM_039099877
VERSION     NM_001401036.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Dad,A., Dobrovolsky,V.N., Heflich,R.H. and Revollo,J.R.
  TITLE     Molecular analysis of GPI-anchor biosynthesis pathway genes in rat
            strains used for the Pig-a gene mutation assay
  JOURNAL   Mutat Res Genet Toxicol Environ Mutagen 858-860, 503256 (2020)
   PUBMED   33198937
  REMARK    GeneRIF: Molecular analysis of GPI-anchor biosynthesis pathway
            genes in rat strains used for the Pig-a gene mutation assay.
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Labash,C., Carlson,K., Avlasevich,S.L., Berg,A., Bemis,J.C.,
            MacGregor,J.T. and Dertinger,S.D.
  TITLE     Induction of Pig-a mutant erythrocytes in male and female rats
            exposed to 1,3-propane sultone, ethyl carbamate, or thiotepa
  JOURNAL   Mutat Res Genet Toxicol Environ Mutagen 782, 24-29 (2015)
   PUBMED   25868128
  REMARK    GeneRIF: Data suggest the use of both sexes for
            phosphatidylinositolglycan class A protein Pig-a gene mutation
            studies.
REFERENCE   3  (bases 1 to 2560)
  AUTHORS   Avlasevich,S.L., Phonethepswath,S., Labash,C., Carlson,K.,
            Torous,D.K., Cottom,J., Bemis,J.C., MacGregor,J.T. and
            Dertinger,S.D.
  TITLE     Diethylnitrosamine genotoxicity evaluated in sprague dawley rats
            using pig-a mutation and reticulocyte micronucleus assays
  JOURNAL   Environ Mol Mutagen 55 (5), 400-406 (2014)
   PUBMED   24574022
  REMARK    GeneRIF: Pig-a mutation is associated with diethylnitrosamine
            genotoxicity.
REFERENCE   4  (bases 1 to 2560)
  AUTHORS   Dertinger,S.D., Phonethepswath,S., Avlasevich,S.L., Torous,D.K.,
            Mereness,J., Cottom,J., Bemis,J.C. and Macgregor,J.T.
  TITLE     Pig-a gene mutation and micronucleated reticulocyte induction in
            rats exposed to tumorigenic doses of the leukemogenic agents
            chlorambucil, thiotepa, melphalan, and 1,3-propane sultone
  JOURNAL   Environ Mol Mutagen 55 (4), 299-308 (2014)
   PUBMED   24449360
  REMARK    GeneRIF: Pig-a gene mutation is associated with micronucleated
            reticulocyte induction.
REFERENCE   5  (bases 1 to 2560)
  AUTHORS   Dertinger,S.D., Phonethepswath,S., Avlasevich,S.L., Torous,D.K.,
            Mereness,J., Bryce,S.M., Bemis,J.C., Bell,S., Weller,P. and
            Macgregor,J.T.
  TITLE     Efficient monitoring of in vivo pig-a gene mutation and chromosomal
            damage: summary of 7 published studies and results from 11 new
            reference compounds
  JOURNAL   Toxicol Sci 130 (2), 328-348 (2012)
   PUBMED   22923490
  REMARK    GeneRIF: Data demonstrate the value of integrating Pig-a mutation
            and micronucleus endpoints into in vivo toxicology studies.
REFERENCE   6  (bases 1 to 2560)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   7  (bases 1 to 2560)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 2560)
  AUTHORS   Murakami,Y., Siripanyaphinyo,U., Hong,Y., Tashima,Y., Maeda,Y. and
            Kinoshita,T.
  TITLE     The initial enzyme for glycosylphosphatidylinositol biosynthesis
            requires PIG-Y, a seventh component
  JOURNAL   Mol Biol Cell 16 (11), 5236-5246 (2005)
   PUBMED   16162815
REFERENCE   9  (bases 1 to 2560)
  AUTHORS   Mortazavi,Y., Merk,B., McIntosh,J., Marsh,J.C., Schrezenmeier,H.
            and Rutherford,T.R.
  CONSRTM   BIOMED II Pathophysiology and Treatment of Aplastic Anaemia Study
            Group
  TITLE     The spectrum of PIG-A gene mutations in aplastic anemia/paroxysmal
            nocturnal hemoglobinuria (AA/PNH): a high incidence of multiple
            mutations and evidence of a mutational hot spot
  JOURNAL   Blood 101 (7), 2833-2841 (2003)
   PUBMED   12424196
REFERENCE   10 (bases 1 to 2560)
  AUTHORS   Kawagoe,K., Kitamura,D., Okabe,M., Taniuchi,I., Ikawa,M.,
            Watanabe,T., Kinoshita,T. and Takeda,J.
  TITLE     Glycosylphosphatidylinositol-anchor-deficient mice: implications
            for clonal dominance of mutant cells in paroxysmal nocturnal
            hemoglobinuria
  JOURNAL   Blood 87 (9), 3600-3606 (1996)
   PUBMED   8611683
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On Feb 1, 2022 this sequence version replaced XM_039099877.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR26360194.364000.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA5760383,
                                    SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-591               JAXUCZ010000021.1  33687027-33687617   c
            592-880             JAXUCZ010000021.1  33686009-33686297   c
            881-1660            JAXUCZ010000021.1  33684051-33684830   c
            1661-1793           JAXUCZ010000021.1  33679298-33679430   c
            1794-1926           JAXUCZ010000021.1  33678342-33678474   c
            1927-2133           JAXUCZ010000021.1  33677995-33678201   c
            2134-2560           JAXUCZ010000021.1  33674923-33675349   c
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq14"
     gene            1..2560
                     /gene="Piga"
                     /note="phosphatidylinositol glycan anchor biosynthesis,
                     class A"
                     /db_xref="GeneID:363464"
                     /db_xref="RGD:1589723"
     exon            1..591
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
     exon            592..880
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1660
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
     CDS             943..2400
                     /gene="Piga"
                     /EC_number="2.4.1.198"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol glycan, class A;
                     N-acetylglucosaminyl-phosphatidylinositol biosynthetic
                     protein; phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A"
                     /codon_start=1
                     /product="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A isoform 1"
                     /protein_id="NP_001387965.1"
                     /db_xref="GeneID:363464"
                     /db_xref="RGD:1589723"
                     /translation="MANRRGGGQGQPPSVSPSPGSSGSLSTDRTCTHNICMVSDFFYP
                     NMGGVESHIYQLSQCLIERGHKVITVTHAYGNRKGVRYLTNGLKVYYLPLRVMYNQST
                     ATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFA
                     DVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPE
                     PFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELNFLIGGEGPKRIILEEVR
                     ERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKV
                     GGIPEVLPENLIILCEPSVKSLCEGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVA
                     ERTEKVYERVSKESVLPMHKRLDRLISHCGPVTGYIFALLAVLSYLFLIFLQWMTPDS
                     VIDVAIDATGPRRAWTHQWPRDKKRDENDKVSKSR"
     exon            1661..1793
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1926
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2133
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2560
                     /gene="Piga"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acgtgtctcg tctgtccttt ccaacgttct cggtcccttc tggacggggt tgggccgttc
       61 ttagaggagc gtggctggct gtggaggagg tgaagcctac cctacgggag gcccagaagt
      121 caggtgcgag ggtctccgta gtcaaatggg gctatgggtg ccttgagggt tggagctggt
      181 tcctaggctg agtttagtag agcgagggat tggaatctta ggacgttttt ccgactccct
      241 ttgcatatgg ccagattttg cctcatcctg gggtggaggc tcatagaccc gggctgaccc
      301 tctttggctc tagtgcaccc gagatgcctc tcagggagtc gtgccttggg actggattaa
      361 agtatgattc tcttgatttc tcagccccgt ccgagctccc tctaaggatc ctcaggagct
      421 tctactaata agggagttta ctggccatga ctgagaattt tgaggacttc ttgactttgt
      481 catttacgtt gcttgagtag ggacgtttta cagtttgctt aacatgtatc gctcggagag
      541 agaattcacc agattttaca gcctctcttc acttatagaa gccaagattc aagtccacac
      601 tgaaagggca cttgtagagg aaagacctac ataagtaaaa gaaggatccc aaaaagagaa
      661 gggagcagtt tgagtataac tacccttgaa taaggaagga gaaacgggat tgtctgcagc
      721 atgagctccg ggtaacactt gaagagagtg ttcttaaaaa gtaagttctg gttccggtac
      781 attttaatgg aaaccacaaa gcagaatcgt gtgcggtgca ctggatcact agctaagctg
      841 tgtagctatc gtcagcccag tttcagcgaa gaaccagatg gtgacagaag acacatttct
      901 taacaggctt gctctgagaa ctgatgcgtg aaccacctca gcatggccaa tagaagagga
      961 ggtgggcaag gccagcctcc ttcagtgtct ccttcccccg gtagctccgg aagtctttcc
     1021 actgatagga cctgcactca taatatatgc atggtgtctg actttttcta cccaaacatg
     1081 ggaggtgtgg aaagccacat ttaccagctc tctcagtgcc tcattgagag ggggcacaaa
     1141 gtcataactg tcacccatgc ttatggaaat cgaaagggcg tccgttacct caccaatggc
     1201 ctcaaagttt actatttgcc tctgagagtc atgtacaacc aatctacagc cacgactctc
     1261 tttcacagtc tgccattgct caggtacata tttgttcggg agagaattac gataatccat
     1321 tcccacagtt ctttctctgc catggcccat gatgctctct tccacgccaa gacaatgggg
     1381 cttcagacag tcttcacaga ccattccctt tttggatttg ctgatgtcag ctcggtgctt
     1441 acaaacaagc ttcttactgt gtctctttgt gacacaaacc acatcatttg tgtctcttac
     1501 actagtaagg agaacactgt actcagagca gcactgaatc ctgaaatagt gtccgtcatt
     1561 cctaacgctg tagatcctac tgacttcact ccagaaccat ttaggaggca tgatagtgta
     1621 ataactgttg ttgttgtcag cagacttgtt tacagaaaag ggactgattt gcttagtggt
     1681 ataatacctg aactctgtca gaaataccaa gaattaaact tcctgattgg aggagaggga
     1741 ccaaagagga tcattttgga agaagtccgg gagagatacc agctgcacga cagggtgcaa
     1801 cttttgggag ctttagaaca caaggatgtt agaaatgtcc tagttcaagg acatattttt
     1861 cttaacacct ccctcacaga agcattctgc atggcaatcg tggaagccgc cagttgtggt
     1921 ttgcaggtcg tcagcacaaa ggttggtggg attcctgaag tccttccaga aaatcttatt
     1981 attttatgtg agccttcagt aaaatctttg tgtgaaggtc tggaaaaagc cattttccaa
     2041 gtgaagtcgg ggacattgcc tgctccagaa aatatccaca atgtagtgaa gactttctac
     2101 acctggcgga atgtggcaga gagaactgaa aaagtgtatg agcgagtgtc aaaggaatcc
     2161 gtgttgccaa tgcacaagag attggacagg ctcatctctc actgtggccc agtgacaggc
     2221 tacatttttg ctttgctggc cgtgctcagc tatctcttcc tcattttcct gcaatggatg
     2281 actccagact ctgtcattga tgttgcaata gatgctactg ggccaaggag agcctggact
     2341 catcaatggc ctcgtgataa gaaaagagat gagaatgata aagtatccaa aagcaggtag
     2401 aagcaagccc aaattaacaa gtcctattag atacttgcaa tagtcctgtt agcatttgaa
     2461 aataattttg ctcattgttt ttgggttttc gagttggttt tgtaaattat gctacctctg
     2521 tatcgttcac ttttgttttt gaaaaaagat tcttatgcaa
//
