LOCUS       NM_001077200            8221 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus piezo-type mechanosensitive ion channel component
            1 (Piezo1), mRNA.
ACCESSION   NM_001077200
VERSION     NM_001077200.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Li,M.J., Li,C.X., Li,J.Y., Gong,Z.C., Shao,B., Zhou,Y.C., Xu,Y.J.
            and Jia,M.Y.
  TITLE     Biomechanism of abnormal stress on promoting osteoarthritis of
            temporomandibular joint through Piezo1 ion channel
  JOURNAL   J Oral Rehabil 51 (10), 1935-1946 (2024)
   PUBMED   38873703
  REMARK    GeneRIF: Biomechanism of abnormal stress on promoting
            osteoarthritis of temporomandibular joint through Piezo1 ion
            channel.
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Feng,S., Yu,Z., Yang,Y., Xiong,Q., Yan,X. and Bi,Y.
  TITLE     Mechanosensitive Piezo1 channels promote neurogenic bladder
            fibrosis via regulating TGF-beta1/smad and Hippo/YAP1 pathways
  JOURNAL   Exp Cell Res 442 (1), 114218 (2024)
   PUBMED   39178981
  REMARK    GeneRIF: Mechanosensitive Piezo1 channels promote neurogenic
            bladder fibrosis via regulating TGF-beta1/smad and Hippo/YAP1
            pathways.
REFERENCE   3  (bases 1 to 8221)
  AUTHORS   Zeng,J., Fang,Z., Duan,J., Zhang,Z., Wang,Y., Wang,Y., Chen,L.,
            Wang,J. and Liu,F.
  TITLE     Activation of Piezo1 by intracranial hypertension induced neuronal
            apoptosis via activating hippo pathway
  JOURNAL   CNS Neurosci Ther 30 (9), e14872 (2024)
   PUBMED   39328029
  REMARK    GeneRIF: Activation of Piezo1 by intracranial hypertension induced
            neuronal apoptosis via activating hippo pathway.
REFERENCE   4  (bases 1 to 8221)
  AUTHORS   Xu,F., Xin,Q., Ren,M., Shi,P. and Wang,B.
  TITLE     Inhibition of piezo1 prevents chronic cerebral
            hypoperfusion-induced cognitive impairment and blood brain barrier
            disruption
  JOURNAL   Neurochem Int 175, 105702 (2024)
   PUBMED   38401846
  REMARK    GeneRIF: Inhibition of piezo1 prevents chronic cerebral
            hypoperfusion-induced cognitive impairment and blood brain barrier
            disruption.
REFERENCE   5  (bases 1 to 8221)
  AUTHORS   Zhang,J., Wang,X., Fu,Z., Xing,C., Wang,Z., Yang,H., Li,J., Liu,M.,
            Dong,L., Zhang,X., Li,Y., Wang,J., Long,J., Liu,J., Wang,S., Li,J.
            and Gao,F.
  TITLE     Long-term simulated microgravity fosters carotid aging-like changes
            via Piezo1
  JOURNAL   Cardiovasc Res 120 (5), 548-559 (2024)
   PUBMED   38271270
  REMARK    GeneRIF: Long-term simulated microgravity fosters carotid
            aging-like changes via Piezo1.
REFERENCE   6  (bases 1 to 8221)
  AUTHORS   Ge,J., Li,W., Zhao,Q., Li,N., Chen,M., Zhi,P., Li,R., Gao,N.,
            Xiao,B. and Yang,M.
  TITLE     Architecture of the mammalian mechanosensitive Piezo1 channel
  JOURNAL   Nature 527 (7576), 64-69 (2015)
   PUBMED   26390154
REFERENCE   7  (bases 1 to 8221)
  AUTHORS   Coste,B., Xiao,B., Santos,J.S., Syeda,R., Grandl,J., Spencer,K.S.,
            Kim,S.E., Schmidt,M., Mathur,J., Dubin,A.E., Montal,M. and
            Patapoutian,A.
  TITLE     Piezo proteins are pore-forming subunits of mechanically activated
            channels
  JOURNAL   Nature 483 (7388), 176-181 (2012)
   PUBMED   22343900
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 8221)
  AUTHORS   Coste,B., Mathur,J., Schmidt,M., Earley,T.J., Ranade,S.,
            Petrus,M.J., Dubin,A.E. and Patapoutian,A.
  TITLE     Piezo1 and Piezo2 are essential components of distinct mechanically
            activated cation channels
  JOURNAL   Science 330 (6000), 55-60 (2010)
   PUBMED   20813920
REFERENCE   9  (bases 1 to 8221)
  AUTHORS   McHugh,B.J., Buttery,R., Lad,Y., Banks,S., Haslett,C. and Sethi,T.
  TITLE     Integrin activation by Fam38A uses a novel mechanism of R-Ras
            targeting to the endoplasmic reticulum
  JOURNAL   J Cell Sci 123 (Pt 1), 51-61 (2010)
   PUBMED   20016066
REFERENCE   10 (bases 1 to 8221)
  AUTHORS   Satoh,K., Hata,M., Takahara,S., Tsuzaki,H., Yokota,H., Akatsu,H.,
            Yamamoto,T., Kosaka,K. and Yamada,T.
  TITLE     A novel membrane protein, encoded by the gene covering KIAA0233, is
            transcriptionally induced in senile plaque-associated astrocytes
  JOURNAL   Brain Res 1108 (1), 19-27 (2006)
   PUBMED   16854388
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000019.1, AB161229.1 and BQ196181.1.
            
            On Sep 3, 2009 this sequence version replaced NM_001077200.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMEA5760383, SAMEA5760386
                              [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-354               JAXUCZ010000019.1  67514684-67515037   c
            355-450             JAXUCZ010000019.1  67482166-67482261   c
            451-577             JAXUCZ010000019.1  67477246-67477372   c
            578-616             JAXUCZ010000019.1  67475908-67475946   c
            617-755             JAXUCZ010000019.1  67475647-67475785   c
            756-918             JAXUCZ010000019.1  67473205-67473367   c
            919-1132            JAXUCZ010000019.1  67472847-67473060   c
            1133-1304           JAXUCZ010000019.1  67472585-67472756   c
            1305-1391           JAXUCZ010000019.1  67472398-67472484   c
            1392-1479           JAXUCZ010000019.1  67472226-67472313   c
            1480-1580           JAXUCZ010000019.1  67471023-67471123   c
            1581-7898           AB161229.1         4-6321
            7899-8221           BQ196181.1         1-323               c
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..8221
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /note="piezo-type mechanosensitive ion channel component
                     1"
                     /db_xref="GeneID:361430"
                     /db_xref="RGD:1308822"
     exon            1..354
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..7898
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /note="protein PIEZO1; membrane protein induced by
                     beta-amyloid treatment; family with sequence similarity
                     38, member A"
                     /codon_start=1
                     /product="piezo-type mechanosensitive ion channel
                     component 1"
                     /protein_id="NP_001070668.2"
                     /db_xref="GeneID:361430"
                     /db_xref="RGD:1308822"
                     /translation="MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWL
                     PGPSRHSIPGHTGRLLRALLCLSLLFLVAHVAFQICLHTMPRLNQLLGQNCNLWANVS
                     QHIGVTRLDLKDIFNTTRLVAPDLGVLVASSLCLGLCGRLTRKARQSQRTQELEEDDI
                     DAAPAAGLQGAPTLATKRRLWLAPRFRITAHWLLVTSGRMLVIVLLALAGIAHPSAFS
                     SVYLMVFLAICTWWSCHFPLSSLGFNTLCVMVSCFGAGHLVCLYCYQTPFVQSVLLPG
                     SLWARLFGLKNFVDIPNCSSPNVLVLNTKHAWPIYVSPGILLLLYYTATSLLKLRKGR
                     FSELRKEIPREDEEHELELDQLEPEPQARGTTQGATPTTTGPDIDNCTVHVLTSQSPV
                     RQRPVRPRLAELKEMSPLHGLGHLILDQSYVCALIAMMVWSIMYHSWLTFVLLLWACL
                     IWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRY
                     PCLDLGAMLLYLLTFWLLLRQFVKEKLLKKRKAPSTLLEVTVSDTEPTQTQTLLRSLG
                     ELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLR
                     VFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFSVSELFSSIL
                     IPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRRLRWAHRQDTVSEAPLLQHQEEE
                     EVFRDDGQSMDGPHQTTQVPEGTASKWGLVADRLLDLASSFSAVLTRIQVFVRCLLEL
                     HVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKM
                     LYQLKIVNPHEYSSNCTEPFPNNTNLQPLEISQSLLYRGPVDPANWFGVRKGYPNLGY
                     IQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQALCADGTRQRLDQDLLSCLKYF
                     INFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIARLWPNYCLF
                     LTLFLLYQYLLCLGMPPALCIDYPWRWSQAIPMNSALIKWLYLPDFFRAPNSTNLISD
                     FLLLLCASQQWQVFSAEQTEEWQRMAGVNTDHLEPLRGEPNPIPNFIHCRSYLDMLKV
                     AVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTTLLQKDTRAQLVLWDCL
                     ILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPKEMKTRDRDC
                     LLPVEEAGIIWDSICFFFLLLQRRVFLSHYFLHVSADLKATALQASRGFALYNAANIK
                     NINFHRQTEERSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSTDPQEPGPDSPGGSSP
                     PRTQWWRPWQDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQMAYQAWVTNA
                     QTVLRQRREQARRDRAEQLASGGDLSPEVELVDVPENEMAGHSHVMQRVLSTMQFLWV
                     LGQATVDGLTRWLRTFTKDHRTMSDVLCAERYLLTQELLRGGEVHRGVLDQLYVSEDE
                     IALSGPVENRDGPSTASSGLGAEEPLSSMTDDTGSPLSTGYNTRSGSEEIITDTGGLQ
                     AGTSLHGSQELLANARTRMRTASELLLDRRLRIPELEEAEQFEAQQGRTLRLLRAMYQ
                     CVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEV
                     MVVTKYLFQFGFFPWNSYIVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHR
                     SQLLCYGLWDHEEDGVPKDHCRSSEKDQEAEEESEAKLESQPETGTGHPEEPVLTGTP
                     KDHIQGKGSVRSKDEIQDPPEDLKPQHRRHISIRFRRRKETQGPKGAAVVEAEHEEGE
                     EGREAAGRKRLRRPREGLKIREKMKAAGRRLQSFCLSLAQSFYQPLRRFFHDILHTKY
                     RAATDVYALMFLADIVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQF
                     GTMVIDRALYLRKTVLGNLAFQVVLVVAIHLWMFFILPAVTERMFRQNAVAQLWYFVK
                     CIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTD
                     TTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAI
                     IWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELS
                     QQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLR
                     FTWNFQRDLAKGGSVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYI
                     RAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWWVIEL
                     QDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSI
                     MFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE"
     exon            355..450
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            451..577
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            578..616
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            617..755
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            756..918
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1132
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1304
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1391
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1479
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1580
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1838
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1950
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2129
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2278
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2461
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2574
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2735
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            2736..2912
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..3038
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3239
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            3240..3444
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3549
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            3550..3703
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            3704..3947
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            3948..4044
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4045..4216
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4217..4306
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4307..4395
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4396..4467
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4468..4568
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4569..4662
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4663..4719
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4720..4892
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            4893..4999
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            5000..5206
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            5207..5465
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            5466..5654
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            5655..5967
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            5968..6103
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            6104..6251
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            6252..6466
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            6467..6625
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            6626..6773
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            6774..6962
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            6963..7055
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            7056..7228
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            7229..7351
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            7352..7431
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            7432..7648
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
     exon            7649..8205
                     /gene="Piezo1"
                     /gene_synonym="Fam38a; Mib"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggagagagc cgctgtgtcg ccgtccggcc cagcacggcc tccgcgagca accgtgagtt
       61 ccgagccgag cgggctgcgc cccagcgcgc gcgggctccg gccgtcggga accagaggga
      121 gcggccgggc cggctggcga ccggagatcg aggcccgcgg gaggtcggag cagcggcgcg
      181 ccgcacgtgc ggagacccgc cctgagtgcc atggcgcgcg gttcctccgg ccctcgcacc
      241 tccctttagc cacggcggcg cgacccgggg cggtcccccg gccgcggggc atggagccgc
      301 acgtgctggg cgccgggctc tactggctgt tgctaccctg cacactcctg gcggcctccc
      361 tgttacgctt caatgctctc tcgttggtct atttgctgtt tctactgctg ctaccctggc
      421 ttccaggccc ctcaagacac agcataccag gtcacacagg tcgcctgctt cgtgcactac
      481 tctgcctcag cctcctcttc ctggtggccc atgttgcctt ccagatatgc ctacatacca
      541 tgcctcgcct gaaccagctt ctgggacaaa actgtaacct ttgggcaaac gtgtctcaac
      601 acataggggt tacaaggctg gacctgaagg acatctttaa caccaccaga ctggtggcac
      661 ctgacctggg agtgctggtg gcatcttccc tttgccttgg cctctgtgga cgcctcacaa
      721 ggaaagcccg gcagagccag cgcactcagg agctggagga ggatgacata gatgctgccc
      781 ccgccgcagg gctgcaggga gcccccaccc tggcaaccaa acgcaggctg tggctggccc
      841 ctcgcttccg gatcacggcg cactggctgc tggtgacctc cggacggatg ctggtcatcg
      901 tgctactggc actcgcaggc atagcccatc cttcggcctt ctccagcgtc tacctgatgg
      961 tgttcctggc catctgcacc tggtggtctt gccactttcc tctcagctcc ctgggcttta
     1021 acaccctctg cgtcatggtg agctgctttg gcgccggcca tcttgtttgt ttatactgct
     1081 atcagacacc atttgtccag agcgtgctac ttcctggcag cctctgggcc aggctatttg
     1141 gtctcaagaa cttcgtagac atccctaact gctccagccc caacgtgctg gtactcaaca
     1201 ctaagcatgc ttggcccatc tacgtgagcc ccggtatcct gctgctgttg tactacacag
     1261 ccacctctct cctgaagctc cgcaagggcc gcttctcaga gctgaggaag gagatcccga
     1321 gggaggatga ggagcatgaa ctggaactgg accagctgga gccagagccc caggctaggg
     1381 gcaccaccca gggtgcgacg cccacgacca ctggacccga cattgacaac tgcaccgtgc
     1441 atgtactaac cagccagagc cccgtccgcc agcgtcctgt acgccccagg ctggctgagc
     1501 tgaaagagat gtcaccacta catggcctgg gccacctcat cctggaccag agctatgtgt
     1561 gtgctctgat cgccatgatg gtgtggagca tcatgtacca cagctggctg accttcgtct
     1621 tgctgctctg ggcctgcctc atctggaccg tgcgcagccg ccaccagttg gctatgctgt
     1681 gctcaccctg catcctgctg tacggactga cactctgctg cctgcggtac gtgtgggcca
     1741 tggagctccc cgagctgccc accaccctgg gccctgtcag tctacatcag ttgggattgg
     1801 agcatacacg ctacccctgc ctggaccttg gtgccatgct gctgtatctg ctcacgttct
     1861 ggctgctcct gcgtcagttc gtgaaggaga agctgctgaa gaagagaaag gcaccttcga
     1921 cgctgctgga ggtcacagtg tccgacacag agcccacaca gacccagaca ctgctgagga
     1981 gcctggggga gctggtcacg ggcatctacg tcaagtactg gatctacgtg tgcgctggga
     2041 tgttcattgt ggtcagcttc gctggccgcc tggtggtcta caaaatcgtc tacatgttcc
     2101 ttttcctgct gtgcctcacg ttgttccagg tctactacac tctgtggagg aagctgctcc
     2161 gagttttctg gtggctcgtg gtggcctaca ccatgctcgt gctcatcgcc gtgtacactt
     2221 tccagttcca ggacttcccc acctactggc gcaacctcac gggcttcaca gatgaacagt
     2281 tgggcgacct gggcctggag cagttcagcg tgtcggagct cttttccagc atcctcatcc
     2341 ctggcttctt cctgctggcc tgcatcctgc agctgcacta cttccacagg ccgttcatgc
     2401 agctcaccga cctggagcac gtgcccccgc caggcacacg ccgccttcgc tgggctcaca
     2461 ggcaggatac agtgagcgag gcccctctgc ttcagcatca ggaggaggag gaagtcttca
     2521 gggatgacgg gcagagcatg gatgggcccc accagaccac acaggtccct gagggtacag
     2581 ccagcaagtg gggcctggtg gccgaccggc tgctggacct agcgtccagc ttctccgccg
     2641 tcctcacccg catccaggtg ttcgtacgat gtttgctcga gcttcacgtc ttcaagctgg
     2701 tggctctcta cactgtctgg gtggccctga aagaagtgtc cgtgatgaac ctgctgctgg
     2761 tggtgctgtg ggccttcgcc ctgccctacc cgcgcttccg gcccatggcc tcctgcctgt
     2821 ccaccgtgtg gacctgtatc atcatcgtgt gcaagatgct gtatcagctg aagattgtca
     2881 acccacacga gtactccagt aactgcaccg agccctttcc caacaatacc aacttgcagc
     2941 ccttggagat cagccagtct ctgctgtacc gtggccccgt tgaccctgcc aactggtttg
     3001 gggtgcggaa gggttaccca aacttgggct atatccagaa ccacctgcag atccttctgc
     3061 tgctggtgtt tgaggctgtg gtgtaccggc gccaagagca ctaccgccgg cagcaccagc
     3121 aggcccctct gcccgcccag gctttgtgcg ctgatggcac ccgccagagg ctggaccagg
     3181 acctactcag ctgcctcaaa tatttcatca acttcttctt ctacaaattc gggctggaga
     3241 tctgcttctt gatggccgtg aatgtcatcg gacaacgcat gaacttcatg gtgatcttac
     3301 acggctgctg gttggtggcc atccttacac gccggcgccg tgaggccatc gcccgcctct
     3361 ggcccaatta ctgtctgttc ctcacgctgt tcctgctgta ccagtacctg ctgtgcctgg
     3421 gcatgcctcc cgcactgtgc atcgactacc cgtggcgctg gagccaggcc atccccatga
     3481 attctgcgct catcaagtgg ctgtacctcc ctgacttctt cagggccccc aactccacca
     3541 accttatcag tgacttcctc ctgctactct gcgcctccca acagtggcag gtgttctccg
     3601 cagagcaaac ggaagaatgg cagcgcatgg ctggcgttaa cactgaccac ctggagcccc
     3661 tgcgtgggga gcccaaccct atccccaact tcatccactg caggtcctat ctggatatgc
     3721 tgaaggtggc cgtcttccgc tacctgttct ggctggtgct ggtggtcgtg tttgtcacag
     3781 gggccacccg catcagcatc tttgggctgg ggtacctgct ggcctgcttc tacctgctgc
     3841 tctttggcac caccctgctg cagaaggaca cacgagccca gctcgtgctg tgggactgcc
     3901 tcatcctcta caacgtcacc gtcatcatct ctaagaatat gttgtcgctc ctgtcctgtg
     3961 tcttcgtgga gcaaatgcag agcaacttct gctgggtcat ccagctcttc agcctcgtgt
     4021 gcacagtcaa aggctactat gaccccaaag agatgaagac cagggaccgg gattgcctgc
     4081 tgcctgtgga ggaggccggg atcatctggg acagcatctg cttcttcttc ctgctcctac
     4141 aacggcgcgt ctttctcagc cactacttcc tgcacgtcag tgctgacctg aaggccacgg
     4201 ccctgcaggc ctccaggggt tttgctctct acaatgcggc caacataaag aacatcaact
     4261 tccaccgtca gaccgaggag aggtccctgg cccagctgaa aagacagatg aagcgcatcc
     4321 gtgccaaaca ggagaaatac cggcagagcc aggcaagccg tggtcaactc cagtccacag
     4381 accctcagga gccagggcct gacagcccag ggggttcctc cccgccacgg acacagtggt
     4441 ggcgcccctg gcaggaccac gccacagtca tccactctgg tgactacttc ctgtttgagt
     4501 cggacagtga ggaggaagag gaggccctgc ctgaggaccc caggcctgca gctcagagtg
     4561 ccttccagat ggcataccag gcatgggtaa ccaacgccca gacagtgcta aggcagcgtc
     4621 gtgaacaggc acggcgggat cgggcagaac agctggcttc tggaggcgac ttgagtcctg
     4681 aggtggagct ggtagacgtc ccagagaatg agatggcagg ccatagccat gtgatgcagc
     4741 gcgtgctgag taccatgcag ttcctgtggg tgctgggcca ggccacagtg gacgggctga
     4801 cgcgctggtt gcgtacgttc acgaaggacc accgcaccat gagtgatgtg ctgtgtgcag
     4861 agcgttacct gctcacccag gagcttcttc ggggtggaga ggtacaccga ggggtactgg
     4921 accagcttta cgtgagcgaa gatgagatcg cactgtcagg tcctgtggag aaccgtgatg
     4981 gacccagcac agcctctagc gggctgggag ctgaagagcc tttgagtagc atgacagacg
     5041 acaccggcag cccccttagc actggctaca acactcgcag tggtagtgag gagattatca
     5101 ccgacactgg gggcctccag gctgggacct ccctgcacgg ctcccaagag cttctagcca
     5161 atgctcgtac ccggatgcgc acagccagtg agctgctgct ggacaggcgc ctgcgtatcc
     5221 ctgagctgga ggaggccgag cagtttgagg cacagcaggg ccggacgctg cggctgctca
     5281 gagccatgta ccagtgtgtg gcggcccact cagagcttct ctgttacttc atcatcatcc
     5341 ttaaccacat ggtgacagcc tcggctgcct ccctggtgct gcctgtgctt gtgttcctct
     5401 gggccatgct gaccatcccg aggcctagca agcgcttctg gatgacagct atcgtcttca
     5461 ctgaggtcat ggtggtcacc aaatacttgt tccagtttgg cttcttcccc tggaacagct
     5521 acatagtgct tcgacgctac gagaacaagc cctacttccc tccgcgcatc ctgggcctcg
     5581 agaaaacaga cagctatatc aagtacgacc tggtgcaact catggccctc ttcttccacc
     5641 gctcgcagtt actgtgctat ggcctctggg accatgagga ggatggtgtc cccaaggacc
     5701 attgcaggag tagcgaaaag gaccaggaag ctgaggaaga gtcagaggct aagctggaat
     5761 cgcagcctga gacgggcact gggcatcctg aggagcccgt gttgactggt acccccaagg
     5821 accacatcca agggaaagga agtgttaggt ccaaggatga gatccaagat cccccagagg
     5881 accttaagcc ccagcacagg agacacatca gcatacgctt caggaggagg aaggagactc
     5941 aaggacccaa aggagcagca gtcgtggagg ctgagcacga ggagggagag gaaggaagag
     6001 aagctgcagg gagaaagagg ctgcgtcgcc ctcgagaagg gttgaagatt cgggagaaaa
     6061 tgaaggcagc tggacgccgg ctgcagagct tctgtctgtc actggcccag agcttttacc
     6121 aacccctgcg gcgtttcttc catgacattc tgcacaccaa gtaccgagcg gccaccgacg
     6181 tctatgccct catgttcctg gctgacattg tcgacattgt cgtcatcatc ttcggttttt
     6241 gggcttttgg gaagcactcc gcggccacgg acattgcatc ctcactgtca gatgaccagg
     6301 tgccacaggc cttcctgttc atgctgctgg tccagttcgg caccatggtc atagaccgcg
     6361 ccctctacct gcgtaagact gtactgggca acctggcctt tcaggtggtc ctggtggtgg
     6421 ccattcacct ctggatgttt ttcatcttac cggctgtcac cgagaggatg ttcagacaga
     6481 acgcggtggc ccagctgtgg tacttcgtga agtgcattta ctttgccctg tccgcctacc
     6541 agatccgctg tggctacccc acccgtatct tgggcaactt cctcaccaag aagtacaatc
     6601 atctaaacct cttccttttc caggggttcc gtctagtgcc attcctggtg gagctgcggg
     6661 ccgtcatgga ctgggtatgg actgacacca cgctgtccct gtccaactgg atgtgtgtgg
     6721 aagacatcta tgccaacatc tttatcatca agtgcagccg agagacagag aagaaatacc
     6781 cccagcccaa gggacagaag aagaagaaaa tcgtcaagta tggcatggga ggtctcatca
     6841 tcctcttcct cattgccatc atctggttcc ctctgctctt catgtcactg atccgctctg
     6901 tggttggtgt cgtcaaccag cccattgatg tcactgttac cctcaagtta ggtggctacg
     6961 agccactgtt caccatgagc gcccagcagc catccatagt gccattcact cccgaggact
     7021 atgaggagct gtcccagcag tttgacccct acccactagc catgcagttc attagccagt
     7081 acagtcctga ggacatcgtc actgcacaga ttgagggcag ctcgggggcc ctgtggcgca
     7141 tcagcccgcc cagccgggcc cagatgaagc acgagctgta caacggcaca gccgacatca
     7201 cactgcgctt cacctggaat ttccaaaggg acctggccaa gggcggctct gtggagtata
     7261 ccaacgagaa gcacaccttg gagctggccc ccaacagtac ggcacgaagg cagctggccc
     7321 aactgctcga gggcagacct gaccagtcag tggtcattcc ccacctcttc cccaagtaca
     7381 ttcgtgcacc caatgggcct gaagccaatc ctgtgaagca actgcagcca gacgaggaag
     7441 aggactacct tggtgtgcgt atccagctgc ggagagagca agtgggcaca ggaacctctg
     7501 gggagcaagc aggcacaaag gcctccgact tcctcgagtg gtgggtgatt gagctgcagg
     7561 actgccaggc cgagtgcaac ctgctgccca tggtcatctt cagtgacaag gtcagcccgc
     7621 ctagcctggg cttcctggcc ggctacggga ttgtggggct ctacgtctct atcgtgctgg
     7681 tcgttggcaa gtttgtgcgg ggcttcttca gtgatatctc acactccatc atgttcgagg
     7741 agctgccgtg cgtggaccgc atcctcaagc tgtgccagga catcttcttg gtacgggaga
     7801 cccgcgagct ggagctggag gaggaactct atgccaagct catattcctg taccgatcac
     7861 cagagaccat gatcaagtgg acgcgtgaga aggagtagga gcccagcccc tgggcaccgg
     7921 gaatgaagga gctgccccag ggcagcacgg ccaccagggc tggcagagtg tctgcctgag
     7981 gccgcgagct gtgacacgtc ctggcctacg tcaaccctga tgatgctgct gtcaaaggga
     8041 cgctctgtcc ctacctgttg cgtccctgcc agttgggctg gaggcccagt gtagcccagg
     8101 gctagacaca ctgggtccgg gggcctggct atcccttccc acaagtactg tagggttttc
     8161 taagtaaaac atttttattt atacaaatgg acggtcatag cccaaaaaaa aaaaaaaaaa
     8221 a
//
