LOCUS       NM_053554               2049 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus phosphatidylinositol binding clathrin assembly
            protein (Picalm), mRNA.
ACCESSION   NM_053554
VERSION     NM_053554.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Catsburg,L.A., Westra,M., van Schaik,A.M. and MacGillavry,H.D.
  TITLE     Dynamics and nanoscale organization of the postsynaptic endocytic
            zone at excitatory synapses
  JOURNAL   Elife 11, e74387 (2022)
   PUBMED   35072626
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Zhao,Z., Sagare,A.P., Ma,Q., Halliday,M.R., Kong,P., Kisler,K.,
            Winkler,E.A., Ramanathan,A., Kanekiyo,T., Bu,G., Owens,N.C.,
            Rege,S.V., Si,G., Ahuja,A., Zhu,D., Miller,C.A., Schneider,J.A.,
            Maeda,M., Maeda,T., Sugawara,T., Ichida,J.K. and Zlokovic,B.V.
  TITLE     Central role for PICALM in amyloid-beta blood-brain barrier
            transcytosis and clearance
  JOURNAL   Nat Neurosci 18 (7), 978-987 (2015)
   PUBMED   26005850
  REMARK    Erratum:[Nat Neurosci. 2024 Jan;27(1):208. doi:
            10.1038/s41593-023-01509-y.. PMID: 37985802]
REFERENCE   3  (bases 1 to 2049)
  AUTHORS   Miller,S.E., Mathiasen,S., Bright,N.A., Pierre,F., Kelly,B.T.,
            Kladt,N., Schauss,A., Merrifield,C.J., Stamou,D., Honing,S. and
            Owen,D.J.
  TITLE     CALM regulates clathrin-coated vesicle size and maturation by
            directly sensing and driving membrane curvature
  JOURNAL   Dev Cell 33 (2), 163-175 (2015)
   PUBMED   25898166
REFERENCE   4  (bases 1 to 2049)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2049)
  AUTHORS   Kanatsu,K., Morohashi,Y., Suzuki,M., Kuroda,H., Watanabe,T.,
            Tomita,T. and Iwatsubo,T.
  TITLE     Decreased CALM expression reduces Abeta42 to total Abeta ratio
            through clathrin-mediated endocytosis of gamma-secretase
  JOURNAL   Nat Commun 5, 3386 (2014)
   PUBMED   24577224
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2049)
  AUTHORS   Ford,M.G., Pearse,B.M., Higgins,M.K., Vallis,Y., Owen,D.J.,
            Gibson,A., Hopkins,C.R., Evans,P.R. and McMahon,H.T.
  TITLE     Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the
            nucleation of clathrin lattices on membranes
  JOURNAL   Science 291 (5506), 1051-1055 (2001)
   PUBMED   11161218
REFERENCE   7  (bases 1 to 2049)
  AUTHORS   Kim,H.L. and Kim,J.A.
  TITLE     Purification of clathrin assembly protein from rat liver
  JOURNAL   Exp Mol Med 32 (4), 222-226 (2000)
   PUBMED   11190274
REFERENCE   8  (bases 1 to 2049)
  AUTHORS   Kim,J.A., Kim,S.R., Jung,Y.K., Woo,S.Y., Seoh,J.Y., Hong,Y.S. and
            Kim,H.L.
  TITLE     Properties of GST-CALM expressed in E. coli
  JOURNAL   Exp Mol Med 32 (2), 93-99 (2000)
   PUBMED   10926122
REFERENCE   9  (bases 1 to 2049)
  AUTHORS   Kim,H.L. and Lee,S.C.
  TITLE     Molecular cloning of clathrin assembly protein gene (rCALM) and its
            differential expression to AP180 in rat brain
  JOURNAL   Exp Mol Med 31 (4), 191-196 (1999)
   PUBMED   10630373
REFERENCE   10 (bases 1 to 2049)
  AUTHORS   Tebar,F., Bohlander,S.K. and Sorkin,A.
  TITLE     Clathrin assembly lymphoid myeloid leukemia (CALM) protein:
            localization in endocytic-coated pits, interactions with clathrin,
            and the impact of overexpression on clathrin-mediated traffic
  JOURNAL   Mol Biol Cell 10 (8), 2687-2702 (1999)
   PUBMED   10436022
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF041374.1.
            
            On Aug 30, 2012 this sequence version replaced NM_053554.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF041374.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132266
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2049              AF041374.1         2-2050
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..2049
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /note="phosphatidylinositol binding clathrin assembly
                     protein"
                     /db_xref="GeneID:89816"
                     /db_xref="RGD:621054"
     exon            1..154
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1947
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /note="clathrin-assembly lymphoid myeloid leukemia
                     protein; clathrin-assembly lymphoid leukemia protein;
                     rCALM"
                     /codon_start=1
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein"
                     /protein_id="NP_446006.1"
                     /db_xref="GeneID:89816"
                     /db_xref="RGD:621054"
                     /translation="MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDY
                     LIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNT
                     LFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMN
                     TEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINL
                     LEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLD
                     ALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQAR
                     LKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPND
                     LLDLQQPTFHPSVHAMSAAPQVASTWGDAVDDAIPSLNPFLTKSSGDVHLPISSDVST
                     FTTRTPTHEMFVGFSPSPVTQPHPSAGLNVDFESVFGNKSTNVAVDSGGGLLKPTVAS
                     QNQSLPVAKLPPNKLVSDDLDSSLANLVGNLGIGNGTTKNDVSCSQPGEKKLTGGSNW
                     QPKVAPTTAWSAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMR
                     PPNPFGPVPGAQIQFM"
     exon            155..297
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            298..373
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            374..476
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            477..570
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            571..682
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            683..789
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            790..831
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            832..917
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1041
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1178
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1282
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1411
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1519
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1636
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1667
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1767
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1827
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1932
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2049
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggggtggcg gacgagctgc agagatgtct ggccagagcc tgacggaccg aatcaccgcg
       61 gcccagcaca gtgtcactgg ctccgcggta tctaagacag tatgcaaggc cacgacccac
      121 gagatcatgg gccccaagaa aaagcacctg gactacttaa ttcagtgtac aaatgagatg
      181 aatgtgaata tcccacagtt ggcagacagt ttgtttgaaa gaactactaa tagtagttgg
      241 gtggtggtct tcaaatcact cattacaact catcatttga tggtgtatgg aaacgagcgt
      301 ttcattcagt atttggcttc aagaaacaca ttgtttaact taagcaactt tttggataaa
      361 agtggattgc aaggatatga tatgtctaca tttattagac gatatagtag gtacctaaat
      421 gaaaaggcag tttcatacag acaagttgca ttcgatttca caaaagtgaa gagaggagct
      481 gatggagtta tgagaacaat gaacacagaa aaactgttaa aaactgtacc aattatccaa
      541 aatcaaatgg atgcacttct tgattttaat gttaatagta atgaacttac aaatggggta
      601 ataaatgctg ccttcatgct cctgttcaaa gatgccatta gactatttgc agcatacaat
      661 gaaggaatta ttaatttatt ggaaaaatat tttgatatga aaaagaacca gtgcaaagaa
      721 ggtcttgaca tctataagaa gtttttgact aggatgacaa gaatctcaga gtttctgaaa
      781 gttgcagagc aagttggaat tgacagagga gatattccag atctttcaca ggcccccagc
      841 agtcttcttg atgctttaga acaacattta gcttccttgg aagggaagaa aatcaaagat
      901 tccacagctg caagcagggc tacaacactt tccaatgcag tctcttcttt ggcaagcact
      961 ggcctatctc tgaccaaagt ggatgaaagg gaaaagcagg cagcattaga ggaagaacag
     1021 gctcgattaa aagcactaaa ggaacagcgt ctaaaagaac ttgcaaagaa accgcatacc
     1081 tctttaacaa ctgcagcctc tcctgtgtcc acctcagcag ggggaataat gactgcacca
     1141 gccatcgaca tattttctac ccctagttct tctaacagca catccaagct gccaaatgac
     1201 ctgcttgatt tgcagcagcc aacctttcat ccatctgtcc atgctatgtc agctgctcct
     1261 caggtagcaa gtacatgggg agatgctgtt gatgatgcca ttccaagctt aaatcctttc
     1321 ctcacaaaaa gtagtggtga tgttcacctt cctatttctt cagatgtatc cacttttact
     1381 actaggacac ctactcatga aatgtttgtt ggattcagtc cttctccggt tacacagcca
     1441 catccttcag ctggccttaa tgttgacttt gaatctgtgt ttggaaataa gtctacgaat
     1501 gttgctgtag attctggtgg tggacttctc aaaccaacag tggcctctca gaaccagagt
     1561 cttcctgttg ccaaacttcc gcctaacaaa ttagtgtctg atgacttgga ttcatcttta
     1621 gccaaccttg tgggcaatct tggcattgga aatggaacca ctaagaatga tgtaagttgc
     1681 agtcaaccag gtgaaaagaa gttaactgga ggatctaact ggcaaccaaa ggtcgcacca
     1741 acaactgcct ggagtgctgc aacaatggca ccccctgtaa tggcctatcc tgctactaca
     1801 ccaacgggca tgataggata tggaattcct cctcagatgg gaagtgtacc tgtaatgaca
     1861 cagccaacct taatatacag ccagcctgtc atgagaccgc caaacccctt tggccctgta
     1921 ccaggagcac agatacagtt tatgtaacta gatggaagag aatggaatta ctccaagaat
     1981 agaagtgcac aggtggcgac tccttacttc cagcaaaatc caaactgctg tctctaagac
     2041 tcttcctcc
//
