LOCUS       NP_446006                640 aa            linear   ROD 09-FEB-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein [Rattus
            norvegicus].
ACCESSION   NP_446006
VERSION     NP_446006.1
DBSOURCE    REFSEQ: accession NM_053554.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 640)
  AUTHORS   Catsburg,L.A., Westra,M., van Schaik,A.M. and MacGillavry,H.D.
  TITLE     Dynamics and nanoscale organization of the postsynaptic endocytic
            zone at excitatory synapses
  JOURNAL   Elife 11, e74387 (2022)
   PUBMED   35072626
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 640)
  AUTHORS   Zhao,Z., Sagare,A.P., Ma,Q., Halliday,M.R., Kong,P., Kisler,K.,
            Winkler,E.A., Ramanathan,A., Kanekiyo,T., Bu,G., Owens,N.C.,
            Rege,S.V., Si,G., Ahuja,A., Zhu,D., Miller,C.A., Schneider,J.A.,
            Maeda,M., Maeda,T., Sugawara,T., Ichida,J.K. and Zlokovic,B.V.
  TITLE     Central role for PICALM in amyloid-beta blood-brain barrier
            transcytosis and clearance
  JOURNAL   Nat Neurosci 18 (7), 978-987 (2015)
   PUBMED   26005850
  REMARK    Erratum:[Nat Neurosci. 2024 Jan;27(1):208. doi:
            10.1038/s41593-023-01509-y.. PMID: 37985802]
REFERENCE   3  (residues 1 to 640)
  AUTHORS   Miller,S.E., Mathiasen,S., Bright,N.A., Pierre,F., Kelly,B.T.,
            Kladt,N., Schauss,A., Merrifield,C.J., Stamou,D., Honing,S. and
            Owen,D.J.
  TITLE     CALM regulates clathrin-coated vesicle size and maturation by
            directly sensing and driving membrane curvature
  JOURNAL   Dev Cell 33 (2), 163-175 (2015)
   PUBMED   25898166
REFERENCE   4  (residues 1 to 640)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 640)
  AUTHORS   Kanatsu,K., Morohashi,Y., Suzuki,M., Kuroda,H., Watanabe,T.,
            Tomita,T. and Iwatsubo,T.
  TITLE     Decreased CALM expression reduces Abeta42 to total Abeta ratio
            through clathrin-mediated endocytosis of gamma-secretase
  JOURNAL   Nat Commun 5, 3386 (2014)
   PUBMED   24577224
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 640)
  AUTHORS   Ford,M.G., Pearse,B.M., Higgins,M.K., Vallis,Y., Owen,D.J.,
            Gibson,A., Hopkins,C.R., Evans,P.R. and McMahon,H.T.
  TITLE     Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the
            nucleation of clathrin lattices on membranes
  JOURNAL   Science 291 (5506), 1051-1055 (2001)
   PUBMED   11161218
REFERENCE   7  (residues 1 to 640)
  AUTHORS   Kim,H.L. and Kim,J.A.
  TITLE     Purification of clathrin assembly protein from rat liver
  JOURNAL   Exp Mol Med 32 (4), 222-226 (2000)
   PUBMED   11190274
REFERENCE   8  (residues 1 to 640)
  AUTHORS   Kim,J.A., Kim,S.R., Jung,Y.K., Woo,S.Y., Seoh,J.Y., Hong,Y.S. and
            Kim,H.L.
  TITLE     Properties of GST-CALM expressed in E. coli
  JOURNAL   Exp Mol Med 32 (2), 93-99 (2000)
   PUBMED   10926122
REFERENCE   9  (residues 1 to 640)
  AUTHORS   Kim,H.L. and Lee,S.C.
  TITLE     Molecular cloning of clathrin assembly protein gene (rCALM) and its
            differential expression to AP180 in rat brain
  JOURNAL   Exp Mol Med 31 (4), 191-196 (1999)
   PUBMED   10630373
REFERENCE   10 (residues 1 to 640)
  AUTHORS   Tebar,F., Bohlander,S.K. and Sorkin,A.
  TITLE     Clathrin assembly lymphoid myeloid leukemia (CALM) protein:
            localization in endocytic-coated pits, interactions with clathrin,
            and the impact of overexpression on clathrin-mediated traffic
  JOURNAL   Mol Biol Cell 10 (8), 2687-2702 (1999)
   PUBMED   10436022
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF041374.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF041374.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132266
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     Protein         1..640
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein"
                     /note="clathrin-assembly lymphoid myeloid leukemia
                     protein; clathrin-assembly lymphoid leukemia protein;
                     rCALM"
                     /calculated_mol_wt=69155
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylserine.
                     /evidence=ECO:0000250|UniProtKB:Q13492; propagated from
                     UniProtKB/Swiss-Prot (O55012.1)"
     Site            16
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q13492; propagated from
                     UniProtKB/Swiss-Prot (O55012.1)"
     Site            20
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q13492; propagated from
                     UniProtKB/Swiss-Prot (O55012.1)"
     Region          24..282
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     Region          221..294
                     /region_name="Interaction with PIMREG.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (O55012.1)"
     Site            303
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q13492; propagated from
                     UniProtKB/Swiss-Prot (O55012.1)"
     Site            315
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q13492; propagated from
                     UniProtKB/Swiss-Prot (O55012.1)"
     Region          543..568
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O55012.1)"
     CDS             1..640
                     /gene="Picalm"
                     /gene_synonym="Calm"
                     /coded_by="NM_053554.2:25..1947"
                     /db_xref="GeneID:89816"
                     /db_xref="RGD:621054"
ORIGIN      
        1 msgqsltdri taaqhsvtgs avsktvckat theimgpkkk hldyliqctn emnvnipqla
       61 dslferttns swvvvfksli tthhlmvygn erfiqylasr ntlfnlsnfl dksglqgydm
      121 stfirrysry lnekavsyrq vafdftkvkr gadgvmrtmn tekllktvpi iqnqmdalld
      181 fnvnsneltn gvinaafmll fkdairlfaa ynegiinlle kyfdmkknqc kegldiykkf
      241 ltrmtrisef lkvaeqvgid rgdipdlsqa psslldaleq hlaslegkki kdstaasrat
      301 tlsnavssla stglsltkvd erekqaalee eqarlkalke qrlkelakkp htslttaasp
      361 vstsaggimt apaidifstp sssnstsklp ndlldlqqpt fhpsvhamsa apqvastwgd
      421 avddaipsln pfltkssgdv hlpissdvst fttrtpthem fvgfspspvt qphpsaglnv
      481 dfesvfgnks tnvavdsggg llkptvasqn qslpvaklpp nklvsddlds slanlvgnlg
      541 igngttkndv scsqpgekkl tggsnwqpkv apttawsaat mappvmaypa ttptgmigyg
      601 ippqmgsvpv mtqptliysq pvmrppnpfg pvpgaqiqfm
//
