LOCUS       NM_053735               3548 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus phosphatidylinositol 4-kinase type 2 alpha
            (Pi4k2a), mRNA.
ACCESSION   NM_053735
VERSION     NM_053735.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Amici,M., Lee,Y., Pope,R.J.P., Bradley,C.A., Cole,A. and
            Collingridge,G.L.
  TITLE     GSK-3beta regulates the synaptic expression of NMDA receptors via
            phosphorylation of phosphatidylinositol 4 kinase type IIalpha
  JOURNAL   Eur J Neurosci 54 (8), 6815-6825 (2021)
   PUBMED   32463939
  REMARK    GeneRIF: GSK-3beta regulates the synaptic expression of NMDA
            receptors via phosphorylation of phosphatidylinositol 4 kinase type
            IIalpha.
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Baumlova,A., Chalupska,D., Rozycki,B., Jovic,M., Wisniewski,E.,
            Klima,M., Dubankova,A., Kloer,D.P., Nencka,R., Balla,T. and
            Boura,E.
  TITLE     The crystal structure of the phosphatidylinositol 4-kinase IIalpha
  JOURNAL   EMBO Rep 15 (10), 1085-1092 (2014)
   PUBMED   25168678
REFERENCE   3  (bases 1 to 3548)
  AUTHORS   Zhou,Q., Li,J., Yu,H., Zhai,Y., Gao,Z., Liu,Y., Pang,X., Zhang,L.,
            Schulten,K., Sun,F. and Chen,C.
  TITLE     Molecular insights into the membrane-associated
            phosphatidylinositol 4-kinase IIalpha
  JOURNAL   Nat Commun 5, 3552 (2014)
   PUBMED   24675427
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3548)
  AUTHORS   Larimore,J., Tornieri,K., Ryder,P.V., Gokhale,A., Zlatic,S.A.,
            Craige,B., Lee,J.D., Talbot,K., Pare,J.F., Smith,Y. and Faundez,V.
  TITLE     The schizophrenia susceptibility factor dysbindin and its
            associated complex sort cargoes from cell bodies to the synapse
  JOURNAL   Mol Biol Cell 22 (24), 4854-4867 (2011)
   PUBMED   21998198
REFERENCE   5  (bases 1 to 3548)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 3548)
  AUTHORS   Guo,J., Wenk,M.R., Pellegrini,L., Onofri,F., Benfenati,F. and De
            Camilli,P.
  TITLE     Phosphatidylinositol 4-kinase type IIalpha is responsible for the
            phosphatidylinositol 4-kinase activity associated with synaptic
            vesicles
  JOURNAL   Proc Natl Acad Sci U S A 100 (7), 3995-4000 (2003)
   PUBMED   12646710
REFERENCE   7  (bases 1 to 3548)
  AUTHORS   Rajebhosale,M., Greenwood,S., Vidugiriene,J., Jeromin,A. and
            Hilfiker,S.
  TITLE     Phosphatidylinositol 4-OH kinase is a downstream target of neuronal
            calcium sensor-1 in enhancing exocytosis in neuroendocrine cells
  JOURNAL   J Biol Chem 278 (8), 6075-6084 (2003)
   PUBMED   12471042
  REMARK    GeneRIF: This enzyme is a downstream target of neuronal calcium
            sensor-1 in enhancing exocytosis in neuroendocrine cells
REFERENCE   8  (bases 1 to 3548)
  AUTHORS   Barylko,B., Wlodarski,P., Binns,D.D., Gerber,S.H., Earnest,S.,
            Sudhof,T.C., Grichine,N. and Albanesi,J.P.
  TITLE     Analysis of the catalytic domain of phosphatidylinositol 4-kinase
            type II
  JOURNAL   J Biol Chem 277 (46), 44366-44375 (2002)
   PUBMED   12215430
  REMARK    GeneRIF: defined the minimal catalytic domain and identified
            conserved amino acid residues required for catalysis
REFERENCE   9  (bases 1 to 3548)
  AUTHORS   Minogue,S., Anderson,J.S., Waugh,M.G., dos Santos,M., Corless,S.,
            Cramer,R. and Hsuan,J.J.
  TITLE     Cloning of a human type II phosphatidylinositol 4-kinase reveals a
            novel lipid kinase family
  JOURNAL   J Biol Chem 276 (20), 16635-16640 (2001)
   PUBMED   11279162
REFERENCE   10 (bases 1 to 3548)
  AUTHORS   Barylko,B., Gerber,S.H., Binns,D.D., Grichine,N., Khvotchev,M.,
            Sudhof,T.C. and Albanesi,J.P.
  TITLE     A novel family of phosphatidylinositol 4-kinases conserved from
            yeast to humans
  JOURNAL   J Biol Chem 276 (11), 7705-7708 (2001)
   PUBMED   11244087
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Dec 1, 2020 this sequence version replaced NM_053735.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY029199.1, SRR22582416.1489544.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-467               JAXUCZ010000001.1  250852154-250852620
            468-668             JAXUCZ010000001.1  250861765-250861965
            669-800             JAXUCZ010000001.1  250863785-250863916
            801-954             JAXUCZ010000001.1  250864287-250864440
            955-1016            JAXUCZ010000001.1  250869760-250869821
            1017-1116           JAXUCZ010000001.1  250871598-250871697
            1117-1250           JAXUCZ010000001.1  250872424-250872557
            1251-1310           JAXUCZ010000001.1  250873034-250873093
            1311-3548           JAXUCZ010000001.1  250876162-250878399
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q54"
     gene            1..3548
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /note="phosphatidylinositol 4-kinase type 2 alpha"
                     /db_xref="GeneID:114554"
                     /db_xref="RGD:620485"
     exon            1..467
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1472
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /EC_number="2.7.1.67"
                     /function="phosphorylates phosphatidylinositol on the D-4
                     position"
                     /note="phosphatidylinositol 4-kinase type II-alpha; 55 kDa
                     type II phosphatidylinositol 4-kinase"
                     /codon_start=1
                     /product="phosphatidylinositol 4-kinase type 2-alpha"
                     /protein_id="NP_446187.1"
                     /db_xref="GeneID:114554"
                     /db_xref="RGD:620485"
                     /translation="MDETSPLVSPERAQPPEYTFPSVSGAHFPQVPGGAVRVAAAGSG
                     PSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAAQAQALAAQAAVAVHAVQTHRERND
                     FPEDPEFEVVVRQAEIAIECSIYPERIYQGSSGSYFVKDSQGRIIAVFKPKNEEPYGN
                     LNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASE
                     TFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRF
                     EAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDYPMDNPNCRDTDWVMV
                     REPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNF
                     VKDLEEDLYELFKKDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVIV
                     ETARSHQRSSSESYTQSFQSRKPFFSWW"
     exon            468..668
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            669..800
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            801..954
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1016
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1116
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1250
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1310
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..3548
                     /gene="Pi4k2a"
                     /gene_synonym="Pi4KII"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagcgcgccg ggttcccgag ccggcggtct gagggatgga cgagacgagc ccgctagtgt
       61 cccccgagcg ggcccaaccc cctgagtaca ccttcccgtc ggtctcggga gctcactttc
      121 cgcaggtacc ggggggcgcg gtccgcgtag cggcggccgg ctccggcccg tccccgccgt
      181 gctctccggg ccacgaccgg gagcggcagc ccctgctgga ccgggcccgg ggcgcggcgg
      241 cgcagggtca gacccacacg gtggcggcgc aggcccaggc cctggccgcc caggcggccg
      301 tggcggtgca cgccgttcag acccaccgcg agcgaaacga cttcccggag gaccccgagt
      361 tcgaggtggt ggtgcggcag gccgagatag ccatcgagtg cagcatctat cccgagcgca
      421 tctaccaggg ctccagtgga agctacttcg tcaaggactc tcaggggagg atcattgctg
      481 tcttcaaacc caagaatgaa gagccatacg ggaaccttaa ccctaagtgg accaaatggc
      541 ttcagaagct gtgctgcccc tgctgctttg gccgagattg ccttgttctc aaccagggct
      601 atctctcaga agcaggggct agcctggtgg accaaaaact ggaactcaac attgtacccc
      661 gtacaaaggt agtatacctg gccagtgaaa ccttcaacta tagtgccatt gaccgagtaa
      721 agtccagggg caagcgactt gcactagaga aagtgccaaa agttgggcag cggtttaacc
      781 gcattgggct accaccaaag gtcgggtcat tccagctctt tgttgaaggc tacaaagatg
      841 cagactattg gctacggcgt tttgaagcag aacctcttcc cgagaacacg aaccgacagc
      901 tactattgca gtttgagcgg ttggtggtcc tggactacat catccgcaac actgaccgag
      961 gcaatgacaa ctggttgatc aaatatgact atccgatgga taatcctaac tgtcgggaca
     1021 cagactgggt gatggtgagg gagcctgtta tcaaggtggc cgccatagac aatggactgg
     1081 ctttcccact gaagcaccct gactcctgga gggcatatcc tttttactgg gcctggttgc
     1141 cacaggcgaa agtcccattc tctcaggaga tcaaagattt gattcttcca aagatttcag
     1201 accctaactt cgtcaaggac ttggaggagg acctatatga actcttcaag aaagatcctg
     1261 gtttcgacag gggccagttc cataagcaga ttgctgtcat gagaggtcag atcctaaatt
     1321 tgacccaggc cctaaaagac aataagagtc ccctgcacct cgtccagatg ccacccgtga
     1381 ttgttgagac ggcccgttct catcagcggt cttcaagtga atcctacaca cagagcttcc
     1441 agagtcggaa gcccttcttt tcatggtggt agcccagagg caggcaggca gaagaaatcc
     1501 tgcaggtgac aggctgggag aaagcctgga gacccaggtt ccaggaagcc agagaagctg
     1561 gtgcacagca gagcctttca gagcccttcc tctttgcttg ccacctccct cagggctttc
     1621 ctaccgaccg ggagaagcac aatcagggac agagtgctcc tggaccttca gagtattggg
     1681 gaggctgatg tccaggcaag gagcccaggt ggctgagagg agcagctccc tcctagcatc
     1741 tggtggctgg ctgggaaagt cccatctctc cctgacaacc ctctccaccc aggttcccta
     1801 atatattctg catcagaaaa aaaacaaaaa caaatttaaa tacttatagg aaaacccata
     1861 ttaatcctct gacatcagaa gcccttaacc tagaacctaa attcgctcag acacaggcgc
     1921 cctgatacct agcagttgtt actccatgaa ctgagctgtc agaagcccat cgctggccca
     1981 gagacagaag ccattccttt ctggttttgc caaattgacc ctcctcttgc cacactggta
     2041 ctgacttctt gtggcaatga gcaggtttgt gttctgcatc tctaagaggc tctgcacaca
     2101 gcccttttct ctgccctgct gtaacttgcc cggcactgag ctgaagtggg caaggctagc
     2161 cttggcagtt gtgttctgct aggagtggtt gtgtaacacg gagataactg acttagccta
     2221 cttgcatgct gggtgctagg agggttctat ggggagcctg gcacagcctc actcctggtc
     2281 cttagtttca tcctatccta tgtttctgtt actctatttt ccccattctt aagtgaggag
     2341 ttcagtttga attctcgagt ggctctcagc agggatgtgg gtcagtaggt tcatgagcag
     2401 ccaggacagc atctctgttc ttcatgtgtc actgctgcct ggaaaaacag gacctgtgtt
     2461 ctgcctccta actgccatgc cccttggctc cctgccttct tgtcctgggc tggtttctgc
     2521 tcagcttctt gaagagaggc cttcaaacag tcccccagtg cctgcccagc tcaatgtgca
     2581 tcttcctgct tccctgtctt ctcccttgcc aggtgtttga accctttcta caccaaagca
     2641 aagaattgac gtcaggaggg agagaaaggg ggccgcgtgt ggctgagagg cagcagaagt
     2701 gtttacaccc tatgctgggg acccttgagc attctcacac actgcaattg gtcagtcagc
     2761 cttggaaaga aacgtcctga aggcctgaaa aaaccaaaga acaggagtga ggagcacagc
     2821 tgtgcctgcc tcaactgctt gcctgaatcc catcgcccaa aggcagggca gaagacacag
     2881 ccttccatca ggtggagcag actgctccct gtccattcca acccaggagc cagcttgctc
     2941 tgggcttcca ctcagcacca tgaatctctg agcactggct atgcatgccc tgtggacaaa
     3001 gtcactgttg tttgcccgac ttgacaatcc catccagccc atgtggactc tggcctcaga
     3061 accacgtttg agagaatccc tgcctcctcc tcctcacagg agccagcagg ggtccagggg
     3121 tccagggtcc tcacagttat cttcacatca gcctgttggc tggagtgctg gagagaactt
     3181 agtgctgcac gagactcttg tgtcctgtcc tgctccagat caggtctgcc atcaggagga
     3241 aattgggtcc aatggaagag ggagccagtg ccaggcccgg tgggcaaggg tacaggcggc
     3301 cttccctgct tccctccctt gaagcctgta ctcaagttgc cttgaatgtg gacttgggag
     3361 tgccaggagc ccagagtgcc aggtacttca gaacagccgt ccctctgcca gcctaaggcc
     3421 tgtcccctat gtctaggaac agaagctggt tggtgagcgc tgctgccctg ttcttctaat
     3481 gcactgtaga aattgtatgt attgtattta aatcattgca aatgtatgaa taacaaattc
     3541 agttctga
//
