LOCUS       NM_021657               6252 bp    mRNA    linear   ROD 09-APR-2024
DEFINITION  Rattus norvegicus PH domain and leucine rich repeat protein
            phosphatase 1 (Phlpp1), mRNA.
ACCESSION   NM_021657 XM_001063791
VERSION     NM_021657.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Li,Y., Li,Z., Liu,J., Liu,Y. and Miao,G.
  TITLE     miR-190-5p Alleviates Myocardial Ischemia-Reperfusion Injury by
            Targeting PHLPP1
  JOURNAL   Dis Markers 2021, 8709298 (2021)
   PUBMED   34868398
  REMARK    GeneRIF: miR-190-5p Alleviates Myocardial Ischemia-Reperfusion
            Injury by Targeting PHLPP1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Zhang,X., Lu,Y., He,N. and Wang,F.
  TITLE     Downregulation of PHLPP1 ameliorates high glucose-evoked injury in
            retinal ganglion cells by attenuating apoptosis and oxidative
            stress through enhancement of Nrf2 activation
  JOURNAL   Exp Cell Res 397 (2), 112344 (2020)
   PUBMED   33164862
  REMARK    GeneRIF: Downregulation of PHLPP1 ameliorates high glucose-evoked
            injury in retinal ganglion cells by attenuating apoptosis and
            oxidative stress through enhancement of Nrf2 activation.
REFERENCE   3  (bases 1 to 6252)
  AUTHORS   Ohwada,W., Tanno,M., Yano,T., Ong,S.B., Abe,K., Sato,T., Kuno,A.,
            Miki,T., Sugawara,H., Igaki,Y. and Miura,T.
  TITLE     Distinct intra-mitochondrial localizations of pro-survival kinases
            and regulation of their functions by DUSP5 and PHLPP-1
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (10), 165851 (2020)
   PUBMED   32480039
  REMARK    GeneRIF: Distinct intra-mitochondrial localizations of pro-survival
            kinases and regulation of their functions by DUSP5 and PHLPP-1.
REFERENCE   4  (bases 1 to 6252)
  AUTHORS   Zhang,M., Wang,X., Liu,M., Liu,D., Pan,J., Tian,J., Jin,T., Xu,Y.
            and An,F.
  TITLE     Inhibition of PHLPP1 ameliorates cardiac dysfunction via activation
            of the PI3K/Akt/mTOR signalling pathway in diabetic cardiomyopathy
  JOURNAL   J Cell Mol Med 24 (8), 4612-4623 (2020)
   PUBMED   32150791
  REMARK    GeneRIF: Inhibition of PHLPP1 ameliorates cardiac dysfunction via
            activation of the PI3K/Akt/mTOR signalling pathway in diabetic
            cardiomyopathy.
REFERENCE   5  (bases 1 to 6252)
  AUTHORS   Hu,H.L., Shiflett,L.A., Kobayashi,M., Chao,M.V., Wilson,A.C.,
            Mohr,I. and Huang,T.T.
  TITLE     TOP2beta-Dependent Nuclear DNA Damage Shapes Extracellular Growth
            Factor Responses via Dynamic AKT Phosphorylation to Control Virus
            Latency
  JOURNAL   Mol Cell 74 (3), 466-480 (2019)
   PUBMED   30930055
  REMARK    GeneRIF: Suppression of host DNA-repair pathways that remove
            TOP2bcc trigger HSV-1 reactivation. Moreover, perturbation of AKT
            phosphorylation dynamics by downregulating the PHLPP1 phosphatase
            led to AKT mis-localization and disruption of DSB-induced HSV-1
            reactivation.
REFERENCE   6  (bases 1 to 6252)
  AUTHORS   Brognard,J. and Newton,A.C.
  TITLE     PHLiPPing the switch on Akt and protein kinase C signaling
  JOURNAL   Trends Endocrinol Metab 19 (6), 223-230 (2008)
   PUBMED   18511290
  REMARK    Review article
REFERENCE   7  (bases 1 to 6252)
  AUTHORS   Hellwinkel,O.J., Rogmann,J.P., Asong,L.E., Luebke,A.M.,
            Eichelberg,C., Ahyai,S., Isbarn,H., Graefen,M., Huland,H. and
            Schlomm,T.
  TITLE     A comprehensive analysis of transcript signatures of the
            phosphatidylinositol-3 kinase/protein kinase B signal-transduction
            pathway in prostate cancer
  JOURNAL   BJU Int 101 (11), 1454-1460 (2008)
   PUBMED   18336616
REFERENCE   8  (bases 1 to 6252)
  AUTHORS   Brognard,J., Sierecki,E., Gao,T. and Newton,A.C.
  TITLE     PHLPP and a second isoform, PHLPP2, differentially attenuate the
            amplitude of Akt signaling by regulating distinct Akt isoforms
  JOURNAL   Mol Cell 25 (6), 917-931 (2007)
   PUBMED   17386267
REFERENCE   9  (bases 1 to 6252)
  AUTHORS   Shimizu,K., Okada,M., Nagai,K. and Fukada,Y.
  TITLE     Suprachiasmatic nucleus circadian oscillatory protein, a novel
            binding partner of K-Ras in the membrane rafts, negatively
            regulates MAPK pathway
  JOURNAL   J Biol Chem 278 (17), 14920-14925 (2003)
   PUBMED   12594205
  REMARK    GeneRIF: SCOP bound through its LRR domain with a subset of K-ras
            in the raft fraction, interfering with the binding of added guanine
            nucleotide to K-Ras.
REFERENCE   10 (bases 1 to 6252)
  AUTHORS   Shimizu,K., Okada,M., Takano,A. and Nagai,K.
  TITLE     SCOP, a novel gene product expressed in a circadian manner in rat
            suprachiasmatic nucleus
  JOURNAL   FEBS Lett 458 (3), 363-369 (1999)
   PUBMED   10570941
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Mar 8, 2021 this sequence version replaced NM_021657.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB023624.1, SRR26643298.50681.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1714              JAXUCZ010000013.1  22823132-22824845
            1715-1911           JAXUCZ010000013.1  22927952-22928148
            1912-2037           JAXUCZ010000013.1  22930605-22930730
            2038-2204           JAXUCZ010000013.1  22953671-22953837
            2205-2351           JAXUCZ010000013.1  22975363-22975509
            2352-2582           JAXUCZ010000013.1  22976023-22976253
            2583-2785           JAXUCZ010000013.1  22990787-22990989
            2786-2846           JAXUCZ010000013.1  22995070-22995130
            2847-2942           JAXUCZ010000013.1  22997388-22997483
            2943-3098           JAXUCZ010000013.1  23000986-23001141
            3099-3299           JAXUCZ010000013.1  23013224-23013424
            3300-3462           JAXUCZ010000013.1  23017838-23018000
            3463-3593           JAXUCZ010000013.1  23025066-23025196
            3594-3698           JAXUCZ010000013.1  23028984-23029088
            3699-3893           JAXUCZ010000013.1  23039040-23039234
            3894-4122           JAXUCZ010000013.1  23040986-23041214
            4123-6252           JAXUCZ010000013.1  23043490-23045619
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13p11"
     gene            1..6252
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /note="PH domain and leucine rich repeat protein
                     phosphatase 1"
                     /db_xref="GeneID:59265"
                     /db_xref="RGD:621308"
     exon            1..1714
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..5346
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /EC_number="3.1.3.16"
                     /note="SCN circadian oscillatory protein; pleckstrin
                     homology domain-containing family E protein 1; PH
                     domain-containing family E member 1; pleckstrin homology
                     domain-containing family E member 1; pleckstrin homology
                     domain containing, family E (with leucine rich repeats)
                     member 1; PH domain leucine-rich repeat-containing protein
                     phosphatase 1; suprachiasmatic nucleus circadian
                     oscillatory protein; Circadian Oscillatory Protein (SCOP)"
                     /codon_start=1
                     /product="PH domain leucine-rich repeat protein
                     phosphatase 1"
                     /protein_id="NP_067689.2"
                     /db_xref="GeneID:59265"
                     /db_xref="RGD:621308"
                     /translation="MEPAAAAPAQRLADPTGEDRAPAAAAAAEGGRSPDSVLSAAAPS
                     GGNGGAAREEAPCEAPPGPLPGRAGGTGRRRRRGVPQPAAGGAAPVTAAGGGANSLLL
                     RRGRLKRNLSAAASSSSSPSSASSAAGGLPASCSASASLCTRSLDRKTLLQKHRQLLQ
                     LQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLLQLGHKGGGV
                     VKVLGHGPPPAAAPAASDQTPATELGRDVEPPPSSSTVGAVRGPARAPPADLPLPGGA
                     WTRCAPRVNPAPSDSSPGELFAGGPCSPSRAPRPASDTESFSLSPSAESVSDRLDPYS
                     SGGGSSSSSEELEADPATVLTGPSGPPHHPVRSSQPRPPSPKTSALLQPKAPTGVDGT
                     GLVVGEGPGDDKAVAAAAPGVPLWTPGRIRETVQKTSSPPSLYVQLHGETTRRLEADE
                     KPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNV
                     RKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSSSGKMHVLPLIGGKVEEVKKHQHCLA
                     FSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQD
                     LTHLNLKQNFLRQNPSLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAEL
                     NVSCNALREVPAAVGAMQNLQTFLLDGNFLQSLPAELENMHQLSYLGLSFNEFTDIPE
                     VLEKLTAVDKLCMAGNCMETLRLQALRRMPHIKHVDLRLNILRKLITDEVDFLQHVTQ
                     LDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNICGYFLKALYASSNELVQLDVYPVP
                     NYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNR
                     LARLPERLERTSVEVLDVQHNQIIELPPNLLMKADSLRFLNASANKLETLPPATLSEE
                     TSSILQELYLTNNSLTDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDI
                     SGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEVKCVDLSCNELSEITLPEN
                     LPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSAGDASGAPAVWSHGYTEASG
                     VKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEE
                     YMVNTFIVMQRKLGTAGQKLGGAAVLCHIRHDPVDLGGSFTLTSANVGKCQTVLCRNG
                     KPLSLSRSYTMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHV
                     QSVLLTPQDEFFILGSKGLWDSLSIEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSI
                     SAVVVQLSVTEDSFCCCELSVGGSMPPPSPGIFPPSVSMVIKDRPSDGLGVPSSSSGM
                     ASEISSELSTSEMSSEVGSTASDEPPSGALSESSPAYPSEQRCMLHPVCLSNSFQRQL
                     SSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLLQVPAE
                     ASDEGIVISANEDESGLSKKTDISAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRK
                     PGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQQQQQQQQQPPPPP
                     QPPQAQAQAQAQAQRPFQMDHLPDCYDTPL"
     exon            1715..1911
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2037
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2204
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2351
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2582
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2785
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2846
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..2942
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            2943..3098
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3299
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3462
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            3463..3593
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            3594..3698
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            3699..3893
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..4122
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
     exon            4123..6252
                     /gene="Phlpp1"
                     /gene_synonym="Phlpp; Plekhe1; Scop"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaccagcgg ggagggtgtg tgtggggggg gcggtggcgg cgaaacgggc gggcggcgcg
       61 cgggatggaa caccgagaca ccgacggaat gctccggatc gccacataat cctctggaac
      121 agccgcgccg aacgccatcg gcgtctccgt tctccgcgcg ccgctgccgc cgccgtctcc
      181 caccttcgcc tcacctcctc ctcctccgct gcctccggag ctgggggggc gacgtgaaac
      241 cccgctacga tggagcccgc cgccgcagcc ccggcacagc gactcgcgga ccccacgggt
      301 gaggaccgag ccccggcggc ggcggcggcg gccgagggcg gacggagtcc ggattctgtg
      361 ctgagcgcgg cggccccgag cggcgggaac ggcggcgcgg cgcgagagga ggccccctgc
      421 gaggcgccgc cggggccgct gcccggccgc gcgggaggca ccggacgcag gcgtcggcgc
      481 ggggtgcccc agcccgcggc cggcggggcc gccccggtga ccgcggccgg cggcggcgcc
      541 aactcgctgc tgctgaggag agggcggctg aagaggaatc tgtcggccgc cgcttcgtcg
      601 tcctcatcgc cgtcctcggc gtcttcggcc gctggcggcc tcccggcgtc ctgctcggcc
      661 tcggcgtcgc tgtgcacccg gagtctggac aggaagacgc tgctccagaa gcaccggcag
      721 ctgcttcagc tgcagccgtc ggaccgggac tgggtgaggc accagctcca gcggggctgt
      781 gtgcacgtct ttgaccgcca catggcctcg tcctacctgc gcccagtgct ctgcacgctg
      841 gacaccacag ccgccgaggt ggccgcccgg cttctgcagc tgggccacaa aggcggcggg
      901 gtggtgaagg tgctgggcca cgggccacct ccggccgctg ccccggccgc cagcgatcag
      961 accccggcca cagagctcgg aagagacgtg gagccaccgc cctcgagcag cacggtcggt
     1021 gctgtccggg gccctgcgcg cgcgcctcct gccgatctgc cgctgcccgg gggcgcctgg
     1081 acgcgctgtg cacctcgggt caaccctgcg ccctcggact ccagccccgg agagctgttc
     1141 gcgggcggcc cctgttcccc gtcccgcgcc ccgcgcccgg cctccgacac cgagagcttc
     1201 agcctgagcc ccagcgccga gagcgtgtca gaccggctgg acccctatag cagcggcggc
     1261 ggctcctcct cttcgtccga ggaactagaa gctgacccgg ccacggtctt gacggggcct
     1321 tccgggccgc cccaccaccc agtccgctcc tcacagcctc gaccgccttc cccgaagacc
     1381 tcggcgttac tgcagccgaa agccccgacg ggcgtagacg gcactggcct agtggtgggc
     1441 gaaggtcccg gagacgacaa ggcggtggca gctgcagctc caggcgttcc tctgtggact
     1501 ccagggagga tccgggagac cgtgcaaaag acgtcttctc cgccctccct gtatgtacag
     1561 ctccatgggg agactacccg gcgcctggag gcggacgaga agccattgca gatccaaaat
     1621 gactacctct tccaactggg atttggggag ctgtggaggg tgcaggagga aggcatggat
     1681 tcggagattg gctgcctcat ccgattctat gcaggaaagc ctcatagcac tgggagctct
     1741 gagcggattc agctctcggg aatgtacaat gtccgaaaag gcaaaatgca gttgccagtg
     1801 aacagatgga caagacgcca ggtcattctg tgtgggacgt gcttgatagt gtcgtctgtg
     1861 aaagacagct cgagtgggaa gatgcacgtt ctgcctctca ttggtggaaa agtggaagaa
     1921 gtgaaaaagc accagcactg tctagcgttc agctcttccg ggccccagag ccagacttac
     1981 tacatctgct tcgatacttt cacggagtac ttacgatggc tgcgtcaagt ctccaaggtt
     2041 gcatcacaac gcataagctc agtagacctc tcatgttgta gccttgagca tctgcctgcc
     2101 aacctctttt acagccaaga ccttactcat ctcaatttaa aacagaactt cctaaggcag
     2161 aaccccagcc tcccagctgc cagaggactt ggtgaactgc agagattcac caaattgaag
     2221 agccttaacc tttccaataa ccacctagga gcctttccgt cagcagtctg cagcatccca
     2281 accctggcag agctgaatgt gtcttgcaat gccctgcgag aagtcccagc agctgttgga
     2341 gctatgcaga acttacagac gttcttgctg gatggaaatt ttctccagtc ccttcctgct
     2401 gagttggaga acatgcacca gctcagctat ctgggtcttt cctttaatga attcactgac
     2461 attccagagg tgttggagaa gctgactgct gtggataaac tgtgcatggc tgggaactgt
     2521 atggagaccc ttagactaca ggccttaaga aggatgcctc atattaaaca cgtggaccta
     2581 agactgaaca tactcagaaa gcttataaca gatgaagtgg actttctgca acatgtcact
     2641 cagcttgacc tgcgagacaa taaacttggt gatctagatg ctatgatctt caacaacata
     2701 gaagttctgc actgcgagag gaatcagctg gtgacactga acatttgtgg ctatttccta
     2761 aaagcactct atgcttcttc taacgaactc gttcaacttg atgtctaccc agttccaaat
     2821 tatctatcgt acatggatgt ctcaagaaac tgcctagaaa gtgtgcctga gtgggtatgt
     2881 gaaagccgga agttagaagt tttggatatt ggccataatc aaatatgtga acttcctgcc
     2941 cgcttatttt gtaatagtag tctccggaaa ttgctggcag gacacaaccg gttggcaagg
     3001 cttcctgaaa ggctggagag aacatcagtg gaggtcttgg acgtacagca taaccagatc
     3061 attgagctcc caccaaacct tctcatgaag gctgacagcc tgagattcct caatgcgtcc
     3121 gcaaacaaac tggaaaccct gcctccagcc acactttctg aggagacaag cagtatatta
     3181 caggagttgt acttgacaaa caacagcctc actgataagt gtgtgccctt gttaacaggg
     3241 cacccccgcc tgaagatcct acacatggct tataaccggc ttcagagctt cccagcaagt
     3301 aaaatggcga aactggagga gcttgaagaa atcgatatca gtgggaataa gctgaaagcc
     3361 atcccaacaa ccatcatgaa ctgcaggcgc atgcacacgg tgattgctca ctcgaactgc
     3421 atcgaagtct ttcctgaagt catgcagctc ccagaagtca agtgtgtaga tctgagctgt
     3481 aatgagctga gtgaaatcac cctaccagaa aacctgccac ctaaactgca ggaactagat
     3541 ctgaccggaa acccacgcct tgccctggac cacaaaagct tggagctgct caataacatc
     3601 cgctgtttca agattgacca gccgtcagca ggagatgcat ctggagcccc agcagtatgg
     3661 agtcatggtt acactgaagc atcgggagta aaaaacaagc tgtgtgtcgc agccctgtct
     3721 gtgaataact tccgtgacaa ccgagaggct ctctacggtg tgtttgatgg agaccggaat
     3781 gtggaagtgc cctaccttct ccagtgcacc atgagtgaca tcttagctga ggagcttcag
     3841 aaaacgaaaa atgaagaaga atacatggtc aatacattta tagtcatgca aaggaaactg
     3901 ggaacggctg ggcagaaact cggtggtgct gctgtcctgt gtcacatcag gcatgaccct
     3961 gtggacctgg gaggatcctt taccctgacc tctgctaatg ttggcaagtg ccaaacggtt
     4021 ctctgtcgaa acgggaagcc gctgtctctg tccaggtcct ataccatgag ctgtgaagaa
     4081 gagcgaaaga ggattaagca gcacaaagcc atcatcactg aggatggcaa ggtcaatgga
     4141 gtgacagagt ccacacgcat cctgggctac accttccttc accccagtgt ggtgcctcgc
     4201 ccccacgtac agtcggtgct tctgactcca caggatgagt ttttcatcct gggcagcaaa
     4261 gggctgtggg acagcctgtc catcgaagag gctgtggaag ccgtgcgcaa cgttccagat
     4321 gctctagccg ccgccaagaa gctatgcacc ctggcccaga gctacggctg ccacgacagc
     4381 atcagtgctg tggtggtgca actcagtgtc acggaagaca gcttctgctg ctgtgagctc
     4441 agtgtgggag ggagcatgcc accgcccagt cccggaatct tcccgccctc cgtcagcatg
     4501 gtgatcaagg accggccctc agatgggctg ggcgtgccgt cctccagcag cggcatggca
     4561 tctgagatca gcagtgaatt gtctacctct gagatgagta gtgaggtggg ctccacagct
     4621 tctgacgagc cgccgtcggg agccctgagt gagagcagcc ccgcctaccc cagcgagcag
     4681 cgctgcatgc tccacccggt ctgcctgtcc aactccttcc agcgccagct gtccagcgcc
     4741 actttctcca gtgcgttctc tgacaacggc cttgacagtg atgacgagga acccattgag
     4801 ggcgtgttca gcaacggcag ccgggtggag gtggaagtgg acatccattg cagccgggcc
     4861 aaggagaagg agagacagca gcacctgctt caggtgccag ctgaagccag tgatgagggc
     4921 attgtcatca gtgccaatga ggatgagtca ggtctgtcca agaagacaga catttctgct
     4981 gtggggacca ttgggcgacg cagggctaac ggctctgtag ctccccagga aaggagccat
     5041 aatgtaatag aggttgccac agatgcacct ctccggaagc cgggaggcta ttttgcagcc
     5101 cctgctcaac cagatccaga tgatcagttt atcatccccc cagagctgga agaggaagtc
     5161 aaagaaatca tgaaacatca ccaggagcag cagcagcagc agcagcagca gcagcagcag
     5221 cagcagcagc agcagcagcc accacctcca ccccagccgc cacaggcaca ggcacaggca
     5281 caggcacagg cacagaggcc cttccagatg gatcacctgc cggactgtta cgacacacca
     5341 ctatgaccca gcctgcgtac tgttttaaac aataaactaa ccagagaggc cgagagagtg
     5401 tcccaggctc acgttaaacc aggggtgttg cttcatgccc tctcccagac actgccccgc
     5461 ctgtctctgc agctcatctc taggctctct ttgtattagt tacttttaat aattaaccac
     5521 ttttcttcta gtgacacttt accaatatga tttaaatttg ttaacttctt tccctaacat
     5581 atcaactgtg taaagacaaa gaacaaaagg tttaatatat tacagagaaa cagtaaatga
     5641 taatgtaata tttttaaaaa tggctttttg ttgttttgtt cgtttggaag gcagggctag
     5701 ttgccgttgc taaatgattt aataatactg taatgtgtat ttctttgtgg ggaaagcttt
     5761 tttttgtctt gaaaatgata gacatttgta gaatatggag actaactcct aggagttgct
     5821 ttactctgtc agtgacttaa gtcactggga ctcactaatt ttctctgaga gaacagttga
     5881 ttgagaaatt tctattgtaa atagctcagt gttacataat gaagcttaaa gtgtttgtag
     5941 tcttggcata gggacacggc cgaaataact gactccttcc tcgctctgca gagcctcccc
     6001 tgccccagcc acctcgccca tgtgcagtgt gggccatttg ctcagtgtgc ttctgtgcag
     6061 ataatgtggc cctgctctcg ggagcccaga accttttaca gacacttttt aattgtgttc
     6121 cttcctgctg ccacaatcag catgactgta tagagtccct tagaccagcc acacatccag
     6181 agttatatgg aaacagaatg cccaaatgga catgtcgtaa tttttgttat aataaatatg
     6241 aaatttacaa gt
//
