LOCUS       NM_022626               4359 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus phosphorylase kinase regulatory subunit alpha 1
            (Phka1), mRNA.
ACCESSION   NM_022626
VERSION     NM_022626.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Kumar,P., Brushia,R.J., Hoye,E. and Walsh,D.A.
  TITLE     Baculovirus-mediated overexpression of the phosphorylase b kinase
            holoenzyme and alpha gamma delta and gamma delta subcomplexes
  JOURNAL   Biochemistry 43 (31), 10247-10254 (2004)
   PUBMED   15287752
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Burwinkel,B., Hu,B., Schroers,A., Clemens,P.R., Moses,S.W.,
            Shin,Y.S., Pongratz,D., Vorgerd,M. and Kilimann,M.W.
  TITLE     Muscle glycogenosis with low phosphorylase kinase activity:
            mutations in PHKA1, PHKG1 or six other candidate genes explain only
            a minority of cases
  JOURNAL   Eur J Hum Genet 11 (7), 516-526 (2003)
   PUBMED   12825073
REFERENCE   3  (bases 1 to 4359)
  AUTHORS   Johnson,J.E., Xie,M., Singh,L.M., Edge,R. and Cornell,R.B.
  TITLE     Both acidic and basic amino acids in an amphitropic enzyme,
            CTP:phosphocholine cytidylyltransferase, dictate its selectivity
            for anionic membranes
  JOURNAL   J Biol Chem 278 (1), 514-522 (2003)
   PUBMED   12401806
REFERENCE   4  (bases 1 to 4359)
  AUTHORS   Garduno,E., Nogues,M., Merino,J.M., Gutierrez-Merino,C. and
            Henao,F.
  TITLE     The content of glycogen phosphorylase and glycogen in preparations
            of sarcoplasmic reticulum-glycogenolytic complex is enhanced in
            diabetic rat skeletal muscle
  JOURNAL   Diabetologia 44 (10), 1238-1246 (2001)
   PUBMED   11692172
REFERENCE   5  (bases 1 to 4359)
  AUTHORS   Brushia,R.J. and Walsh,D.A.
  TITLE     Phosphorylase kinase: the complexity of its regulation is reflected
            in the complexity of its structure
  JOURNAL   Front Biosci 4, D618-D641 (1999)
   PUBMED   10487978
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4359)
  AUTHORS   Cawley,K.C., Akita,C.G., Wineinger,M.A., Carlsen,R.C., Gorin,F.A.
            and Walsh,D.A.
  TITLE     Coordinated expression of phosphorylase kinase subunits in
            regenerating skeletal muscle
  JOURNAL   J Biol Chem 267 (24), 17287-17295 (1992)
   PUBMED   1512265
REFERENCE   7  (bases 1 to 4359)
  AUTHORS   Leijendekker,W.J., Edauw,P., van Hardeveld,C. and Simonides,W.S.
  TITLE     Phosphorylase a formation in protein-glycogen particles isolated
            from fast-twitch muscle of euthyroid and hypothyroid rats
  JOURNAL   Arch Biochem Biophys 274 (1), 120-129 (1989)
   PUBMED   2774570
REFERENCE   8  (bases 1 to 4359)
  AUTHORS   Zander,N.F., Meyer,H.E., Hoffmann-Posorske,E., Crabb,J.W.,
            Heilmeyer,L.M. Jr. and Kilimann,M.W.
  TITLE     cDNA cloning and complete primary structure of skeletal muscle
            phosphorylase kinase (alpha subunit)
  JOURNAL   Proc Natl Acad Sci U S A 85 (9), 2929-2933 (1988)
   PUBMED   3362857
REFERENCE   9  (bases 1 to 4359)
  AUTHORS   Salsgiver,W.J. and Lawrence,J.C. Jr.
  TITLE     Rat skeletal muscle phosphorylase kinase: turnover and control of
            isozyme levels in culture
  JOURNAL   Am J Physiol 250 (3 Pt 1), C365-C373 (1986)
   PUBMED   3953807
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On Apr 28, 2015 this sequence version replaced NM_022626.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF197561.1, SRR26360172.1354978.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                JAXUCZ010000021.1  71778044-71778121   c
            79-237              JAXUCZ010000021.1  71776453-71776611   c
            238-285             JAXUCZ010000021.1  71773102-71773149   c
            286-454             JAXUCZ010000021.1  71770739-71770907   c
            455-537             JAXUCZ010000021.1  71767690-71767772   c
            538-618             JAXUCZ010000021.1  71759236-71759316   c
            619-717             JAXUCZ010000021.1  71756287-71756385   c
            718-864             JAXUCZ010000021.1  71754537-71754683   c
            865-918             JAXUCZ010000021.1  71731991-71732044   c
            919-1041            JAXUCZ010000021.1  71730803-71730925   c
            1042-1137           JAXUCZ010000021.1  71719604-71719699   c
            1138-1245           JAXUCZ010000021.1  71718822-71718929   c
            1246-1324           JAXUCZ010000021.1  71715092-71715170   c
            1325-1459           JAXUCZ010000021.1  71706438-71706572   c
            1460-1569           JAXUCZ010000021.1  71691981-71692090   c
            1570-1714           JAXUCZ010000021.1  71690541-71690685   c
            1715-1793           JAXUCZ010000021.1  71686260-71686338   c
            1794-1963           JAXUCZ010000021.1  71684539-71684708   c
            1964-2140           JAXUCZ010000021.1  71682230-71682406   c
            2141-2232           JAXUCZ010000021.1  71680669-71680760   c
            2233-2372           JAXUCZ010000021.1  71680053-71680192   c
            2373-2529           JAXUCZ010000021.1  71669237-71669393   c
            2530-2609           JAXUCZ010000021.1  71668639-71668718   c
            2610-2688           JAXUCZ010000021.1  71666397-71666475   c
            2689-2818           JAXUCZ010000021.1  71666137-71666266   c
            2819-2920           JAXUCZ010000021.1  71661834-71661935   c
            2921-3036           JAXUCZ010000021.1  71658209-71658324   c
            3037-3075           JAXUCZ010000021.1  71657907-71657945   c
            3076-3126           JAXUCZ010000021.1  71655154-71655204   c
            3127-3297           JAXUCZ010000021.1  71649187-71649357   c
            3298-3351           JAXUCZ010000021.1  71643319-71643372   c
            3352-3552           JAXUCZ010000021.1  71642605-71642805   c
            3553-4359           JAXUCZ010000021.1  71641246-71642052   c
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq22"
     gene            1..4359
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /note="phosphorylase kinase regulatory subunit alpha 1"
                     /db_xref="GeneID:64561"
                     /db_xref="RGD:621522"
     CDS             1..3726
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /EC_number="2.7.11.19"
                     /note="PhK-alpha-subunit; phosphorylase kinase
                     alpha-subunit; phosphorylase kinase alpha M subunit;
                     phosphorylase kinase, alpha 1; phosphorylase B kinase
                     alpha subunit"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit alpha,
                     skeletal muscle isoform"
                     /protein_id="NP_072148.2"
                     /db_xref="GeneID:64561"
                     /db_xref="RGD:621522"
                     /translation="MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDEKDAWV
                     RDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESF
                     KYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLD
                     EVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLF
                     GVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLV
                     EITKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTY
                     FILDGIFSGNTEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDR
                     VPMGKLPHMWGQSLYILGNLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEE
                     IKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTS
                     KLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPI
                     SHTMLDEDGASLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPE
                     GKLYSEDYDEDYDDELDSGNWMDSYDSTRNARCGDEVARYLDHLLAHTGPHPKLTPTS
                     RKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESP
                     QDSQVPSVRVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYTMKGPDWNTE
                     LYEEGGSTVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLT
                     VGLPPEPREKTISAPLPYEALTKLIDEASEGDMNISTLTQEIMVYLAMYMRTQPGLFA
                     EMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVER
                     SVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSPESQTSGGH
                     PSSIDLMSPTFLSPAACISASSGSFPTVCEPQTSKDSRQGQWQRRRRLDGALNRVPIG
                     FYQKVWKILQKCHGLSVEGFVLPSSSTREMTPGEIKFSVHVESVLNRVPQPEYRQLLV
                     EAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICT
                     LLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ"
     exon            1..78
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            79..237
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            238..285
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            286..454
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            455..537
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            538..618
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            619..717
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            718..864
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            865..918
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1041
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1137
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1245
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1324
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1459
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1569
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1714
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1793
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1963
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2140
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2232
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2372
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2529
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..2609
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2688
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2818
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2920
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..3036
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3075
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3126
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3297
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3298..3351
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3552
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3553..4359
                     /gene="Phka1"
                     /gene_synonym="Pcyt1b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgaggagcc ggagtaactc gggggtccgc ctggacggct atgctcggct ggtgcatcag
       61 accatcctgt gccatcagaa tccagtgaca ggcttgcttc cggccagcta tgatgagaaa
      121 gatgcctggg tccgagataa tgtgtatagc atcctggctg tgtggggttt aggcctggca
      181 tatcgaaaga atgcagaccg tgatgaggac aaggcaaagg cctatgaact ggagcagagt
      241 gtagtgaagt taatgagggg actgctgcac tgcatgatca gacaggtgga taaagtagag
      301 tctttcaagt acagtcagag tactaaagat agccttcacg ccaagtacaa caccaaaact
      361 tgtgccacgg tagtgggtga tgaccagtgg ggacacctgc agttggatgc tacttctgtg
      421 tacctgctct tcctagcaca aatgactgcc tcaggactcc atatcatcca cagcctagac
      481 gaagtgaatt ttatacagaa ccttgtgttt tacattgaag ccgcatataa aactgctgac
      541 tttgggatat gggaacgcgg agataagaca aaccaaggca tctcagaatt gaatgcaagt
      601 tcagttggaa tggccaaggc agccctggaa gcactagatg aattagactt gtttggtgtg
      661 aaaggtgggc cgcaatcagt tatccatgtc ctggctgacg aagtacaaca ctgccagtcg
      721 atcctgaatt cactactgcc cagggcttca acatcgaaag aagttgatgc cagtctgctg
      781 tcagtggtct ctttcccagc ctttgctgta gaggacagtc atttggtgga gatcaccaaa
      841 caggagatca tcaccaaact tcagggtcgt tatggttgct gtcgttttct gcgtgatgga
      901 tataaaactc ctaaagagga tcctaatcgc ctatactatg aaccagctga gctgaagcta
      961 tttgaaaaca tagagtgtga atggccactg ttctggacat actttatcct tgatgggatc
     1021 ttcagtggca acacagaaca ggttcaagaa tatagagagg ctcttgatgc agtcctcatc
     1081 aagggcaaaa atggagtccc actacttcca gagctgtaca gtgttcctcc tgacagggtt
     1141 gatgaagaat atcaaaatcc tcacaccgtg gatcgagtcc ctatgggaaa attgcctcac
     1201 atgtggggtc agtccctata tattttagga aacttgatgg cggagggatt tttagctcct
     1261 ggagaaattg atcccctgaa tcgtaggttt tccactgtcc caaagccaga cgtagtggtt
     1321 caagtctcca ttctggctga aacagaagaa atcaaggcca ttttgaagga caaaggaatt
     1381 gatgtggaga ccattgctga agtgtacccc ataagagtac agccagctcg tattctcagc
     1441 cacatttatt ccagtctagg atgcaacagt agaatgaaac tcagtggacg accctacagg
     1501 ctcatgggtg tacttggaac gtcaaaactt tatgacattc gcaaaactat ctttactttc
     1561 actccacagt ttatagacca gcagcagttc tacttggctc tggacaacca gatgatagtc
     1621 gaaatgctcc ggacagatct ctcctacctc tgcagccggt ggaggatgac aggccagccc
     1681 accatcactt tccccatctc acacaccatg ctggatgaag atggagccag cttgaattca
     1741 agtatcctgg cagcactccg gaaaatgcag gatggttatt ttggtggggc caggatccaa
     1801 acaggtaagc tgtcagagtt tttgacaaca tcttgctgca cacacttaag cttcatggac
     1861 cctggacccg agggtaagct gtacagcgaa gattatgatg aagactatga tgatgagctg
     1921 gactctggca actggatgga tagctatgac tcaacacgta atgctcgctg tggtgatgaa
     1981 gttgcccgtt atttagacca ccttttggca cacactggtc cccatcctaa actaactccc
     2041 acctcacgga agggagggct agatcggttc cgagctgctg tgcaaacaac ttgcgactta
     2101 atgtccttgg tggccaaggc caaggagctg catatacaga atgttcacat gtatcttcct
     2161 acaaagttat ttcagccttc tcgcccttca ctcaacttac ttgactcacc tgaatctcca
     2221 caagatagcc aggttccttc tgttcgtgta gaagtccatc ttcctaggga ccagtctggg
     2281 gaagtggact tccagtcatt ggtttcacag ttgaaggaga cctcaagctt acaggagcaa
     2341 gctgatatac tctacatgct atatacaatg aaaggacctg actggaacac tgaattgtac
     2401 gaagaaggag gcagtactgt gagagagctt cttagtgaac tatatgtcaa agtcggtgaa
     2461 attcggcact ggggtctgat ccgatacatc tctgggatct tacggaagaa ggtggaggca
     2521 cttgatgagg cctgcacaga ccttctgtcc taccagaaac acctgacagt agggcttcct
     2581 ccagaacctc gagagaagac catctctgcg cctctaccgt acgaggcact cactaagctg
     2641 atagatgaag ccagcgaagg agacatgaac atctccaccc tgacacagga aataatggtc
     2701 tatcttgcca tgtatatgag aactcagcct ggcctttttg cagaaatgtt ccgacttcga
     2761 attggtttga tcatacaagt tatggcaaca gaactggccc actctctgcg atgttcagct
     2821 gaagaagcca cagagggcct gatgaatctc agtccttcag ccatgaagaa tctcctgcat
     2881 cacatcctca gcggcaaaga gtttggagtg gaacgaagcg ttcgtcccac tgattcaaat
     2941 gtgagtcctg ctatttccat ccatgagatc ggtgctgtcg gagcaacaaa aacagaacga
     3001 actggaatca tgcagttaaa aagtgagatc aagcaggtgg aattccgtag gctgtctgtc
     3061 tcacccgaga gtcagactag tggtggtcat ccctcaagta tagatttgat gtcaccgacc
     3121 tttctgtccc ctgcagcctg tatttctgcg agcagtgggt cctttcctac tgtatgtgag
     3181 ccacagacat ctaaagatag tcgtcaaggc caatggcaac gcaggagaag actagatgga
     3241 gcactaaata gagtaccaat tggattttat caaaaagtgt ggaaaatttt gcagaaatgt
     3301 cacgggcttt ctgtggaagg ttttgttctt ccctcttcaa gcactaggga gatgacccca
     3361 ggcgagatta aattctctgt ccacgtggag tccgtcctga accgtgtgcc tcagccagag
     3421 taccgtcagc ttctggttga agccatcctc gttctcacca tgctggcaga tattgaaatc
     3481 catagcattg ggagcatcat tgctgtggaa aaaatagttc atattgccaa tgacttgttc
     3541 cttcaggaac agaaaaccct cggcgcagat gatatcatgt tggcaaagga tcctgcatct
     3601 ggcatctgta ctcttctgta tgacagtgca cccagtggca gatttggcac catgacctac
     3661 ctctccaagg cagctgccac ctacgtgcag gagtttctgc cacacagcct ctgtgccatg
     3721 cagtgatggc tgtgggttct ggctgctaga atacttcagt atctagcctg tctggttgat
     3781 tgtgcatgga actgacatgt taacacctga tcactccccc atgccccttt cctgccccat
     3841 ccctcccaag aagagacaac tttgctgata atctcactac ttttgaagaa aagaaccctt
     3901 acccggtgac tcacctccag agtgaaagtt caaccgggcc tgcagtcact atccctactc
     3961 ttatggttca taaaagtctg catgtgtttt tattctgtag agctgttacc agcttagttt
     4021 ttaaactcct tttggggagc aggtattcat aacttattcc tgtaagttgg ttttcttctt
     4081 tgttggtttt tacgtgtgca tatgagtggt gggtattgac tgggccatca tttcaagaga
     4141 gtcgatgcta agttggattt tatgctgctg tcaattggaa ctgtctactc taagtgcctt
     4201 tacttaggaa aagccagatc ttgacttcct tcttcactca aaggttgctt cactctgagt
     4261 ggtgaaatgg tggttttgtt gtagaaatac tgcctttcat tgttctctct gtatcggcct
     4321 accgtctctg tggcgaaata ttaaaaaaat gtaagctca
//
