LOCUS       NM_019333               1739 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus
            6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4),
            mRNA.
ACCESSION   NM_019333
VERSION     NM_019333.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Houddane,A., Bultot,L., Novellasdemunt,L., Johanns,M.,
            Gueuning,M.A., Vertommen,D., Coulie,P.G., Bartrons,R., Hue,L. and
            Rider,M.H.
  TITLE     Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis,
            cell proliferation and protein synthesis in mitogen-stimulated
            thymocytes
  JOURNAL   Cell Signal 34, 23-37 (2017)
   PUBMED   28235572
  REMARK    GeneRIF: Increased PFKFB4 expression, but not increased PFKFB3
            Ser461 phosphorylation, plays a role in increasing glycolysis in
            mitogen-stimulated thymocytes.
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Gomez,M., Navarro-Sabate,A., Manzano,A., Duran,J., Obach,M. and
            Bartrons,R.
  TITLE     Switches in 6-phosphofructo-2-kinase isoenzyme expression during
            rat sperm maturation
  JOURNAL   Biochem Biophys Res Commun 387 (2), 330-335 (2009)
   PUBMED   19595670
  REMARK    GeneRIF: The maximum expression of Pfkfb3 was detected before
            puberty, while that of Pfkfb4 was observed in adulthood during rat
            sperm maturation.
REFERENCE   3  (bases 1 to 1739)
  AUTHORS   Manes,N.P. and El-Maghrabi,M.R.
  TITLE     The kinase activity of human brain
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated
            via inhibition by phosphoenolpyruvate
  JOURNAL   Arch Biochem Biophys 438 (2), 125-136 (2005)
   PUBMED   15896703
REFERENCE   4  (bases 1 to 1739)
  AUTHORS   Rider,M.H., Bertrand,L., Vertommen,D., Michels,P.A., Rousseau,G.G.
            and Hue,L.
  TITLE     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head
            with a bifunctional enzyme that controls glycolysis
  JOURNAL   Biochem J 381 (Pt 3), 561-579 (2004)
   PUBMED   15170386
  REMARK    Review article
REFERENCE   5  (bases 1 to 1739)
  AUTHORS   Manzano,A., Perez,J.X., Nadal,M., Estivill,X., Lange,A. and
            Bartrons,R.
  TITLE     Cloning, expression and chromosomal localization of a human testis
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene
  JOURNAL   Gene 229 (1-2), 83-89 (1999)
   PUBMED   10095107
REFERENCE   6  (bases 1 to 1739)
  AUTHORS   Yuen,M.H., Mizuguchi,H., Lee,Y.H., Cook,P.F., Uyeda,K. and
            Hasemann,C.A.
  TITLE     Crystal structure of the H256A mutant of rat testis
            fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Fructose
            6-phosphate in the active site leads to mechanisms for both mutant
            and wild type bisphosphatase activities
  JOURNAL   J Biol Chem 274 (4), 2176-2184 (1999)
   PUBMED   9890980
REFERENCE   7  (bases 1 to 1739)
  AUTHORS   Hirata,T., Kato,M., Okamura,N., Fukasawa,M. and Sakakibara,R.
  TITLE     Expression of human placental-type
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various
            cells and cell lines
  JOURNAL   Biochem Biophys Res Commun 242 (3), 680-684 (1998)
   PUBMED   9464277
REFERENCE   8  (bases 1 to 1739)
  AUTHORS   Hasemann,C.A., Istvan,E.S., Uyeda,K. and Deisenhofer,J.
  TITLE     The crystal structure of the bifunctional enzyme
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals
            distinct domain homologies
  JOURNAL   Structure 4 (9), 1017-1029 (1996)
   PUBMED   8805587
REFERENCE   9  (bases 1 to 1739)
  AUTHORS   Sakata,J., Abe,Y. and Uyeda,K.
  TITLE     Molecular cloning of the DNA and expression and characterization of
            rat testes
            fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   J Biol Chem 266 (24), 15764-15770 (1991)
   PUBMED   1651918
REFERENCE   10 (bases 1 to 1739)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from M64797.1.
            
            [WARNING] On Jul 19, 2024 this sequence was replaced by
            NM_019333.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M64797.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q32"
     gene            1..1739
                     /gene="Pfkfb4"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     4"
                     /db_xref="GeneID:54283"
                     /db_xref="RGD:3310"
     exon            1..27
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            28..131
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1444
                     /gene="Pfkfb4"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="6PF-2-K/Fru-2,6-P2ASE testis-type isozyme;
                     PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase 4;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
                     4-like"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 4"
                     /protein_id="NP_062206.1"
                     /db_xref="GeneID:54283"
                     /db_xref="RGD:3310"
                     /translation="MASPRELTQNPLKKIWMPYSNGRPALHASQRGVCMTNCPTLIVM
                     VGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKI
                     RKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICV
                     DPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKI
                     MDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSP
                     RGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEE
                     MTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQ
                     AVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQ
                     NVDISRPSEEALVTVPAHQ"
     exon            132..248
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            249..345
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            346..412
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            413..487
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            488..544
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            545..666
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            667..874
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1021
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1126
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1256
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1319
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1384
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1739
                     /gene="Pfkfb4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caaataagag aaatctggtt gagtgagcct ggggatggcg tccccacggg aactgaccca
       61 gaatcccctg aagaagatct ggatgccata cagcaatggg aggcccgccc tgcacgccag
      121 ccaacgtggt gtctgcatga ccaactgccc gacactcatc gtcatggtgg gcctgccagc
      181 caggggcaag acctacattt ctaagaagct gacccggtac ctcaactgga ttggcgtgcc
      241 tactcgggaa ttcaacgtgg gtcagtatcg ccgggacatg gtcaagacat acaaatcttt
      301 tgagttcttc ctcccagaca atgaagaggg cctgaaaatc aggaagcagt gtgccttggc
      361 agccctcaat gacgtccgga agttcctcag tgaggagggg ggacacgtgg cggtttttga
      421 tgctaccaat accacccgag aacggagagc gatgatcttt aactttggag aacagaatgg
      481 ctataagacc ttctttgttg aatctatctg tgtggatcct gaggtcattg ctgccaacat
      541 cgtgcaagtg aagctgggca gccctgacta tgtgaaccgg gacagcgacg aagccactga
      601 ggacttcatg aggcgcattg aatgctatga aaactcctat gagtctctgg atgaagaaca
      661 ggacagggat ctgtcctaca tcaagatcat ggacgtgggg cagagttatg tggtgaaccg
      721 tgttgccgac cacatccaga gtcgcatcgt ttattacctc atgaacatcc atgtgacacc
      781 ccgctccatc tacctctgcc ggcacgggga gagtgagctc aacctcaagg gccggattgg
      841 tggggaccct ggactctccc cccggggccg ggagttttcc aagcacctag ctcagttcat
      901 cagtgaccag aacatcaagg acctgaaggt ctggacgagc cagatgaaga ggacgatcca
      961 gacagccgag gcgctgagtg tcccttatga gcagtggaag gtcctcaacg agattgacgc
     1021 gggtgtctgt gaggaaatga cctacgaaga aatccaggac cactacccgc tggagtttgc
     1081 tctgagggac caggacaagt accggtaccg ctacccgaag ggcgagtcct atgaagacct
     1141 ggtgcagcgg ctggagcctg tcatcatgga gctggagagg caggagaacg tgctagtcat
     1201 ttgccaccag gctgtgatgc gttgcctcct ggcctacttc cttgacaagg cagcagaaga
     1261 gctgccctac ctcaaatgcc ccttgcacac agtcctgaag ctcacacctg tggcttacgg
     1321 ttgtaaagtg gagtccatat tcctgaatgt ggcagctgtg aacacacacc gagacagacc
     1381 tcagaatgta gacatatcca ggccttcaga ggaagccctt gtcacagttc ctgctcacca
     1441 atgacccatg gcaggccctg acctctgcgg aggggtcact ccaggccact tcttcctgtt
     1501 tgctgtatga acggtcacca tgcaggaaca ctcatgggtg gctggtaaca ctcagaaccc
     1561 tcagcctaac ccacctgcct ccttggtgac aaccagtgac ggggttgagt gtggctgctg
     1621 acctgctgct gagaatctct gggctacgtg ctctgctgct ggcccctttg ctacagctct
     1681 caagcagtcc gccccccggg ctggctgctt tgtgacattt gcaacattaa atgagacac
//
