LOCUS       NM_057135               2148 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus
            6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3),
            mRNA.
ACCESSION   NM_057135
VERSION     NM_057135.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Liu,Y., Yao,P., Song,Y., Wang,X. and Wang,X.
  TITLE     [Sleep deprivation aggravates cognitive impairment in septic rats
            by activating neuronal glycolytic isoenzyme PFKFB3]
  JOURNAL   Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 35 (3), 287-292 (2023)
   PUBMED   36916342
  REMARK    GeneRIF: [Sleep deprivation aggravates cognitive impairment in
            septic rats by activating neuronal glycolytic isoenzyme PFKFB3].
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Zhu,Z., Liu,Q., Sun,J., Bao,Z. and Wang,W.
  TITLE     Silencing of PFKFB3 protects podocytes against high glucose-induced
            injury by inducing autophagy
  JOURNAL   Mol Med Rep 24 (5) (2021)
   PUBMED   34490476
  REMARK    GeneRIF: Silencing of PFKFB3 protects podocytes against high
            glucoseinduced injury by inducing autophagy.
REFERENCE   3  (bases 1 to 2148)
  AUTHORS   Niu,J., Wu,C., Zhang,M., Yang,Z., Liu,Z., Fu,F., Li,J., Feng,N.,
            Gu,X., Zhang,S., Liu,Y., Fan,R., Li,J. and Pei,J.
  TITLE     kappa-opioid receptor stimulation alleviates rat vascular smooth
            muscle cell calcification via PFKFB3-lactate signaling
  JOURNAL   Aging (Albany NY) 13 (10), 14355-14371 (2021)
   PUBMED   34016793
  REMARK    GeneRIF: kappa-opioid receptor stimulation alleviates rat vascular
            smooth muscle cell calcification via PFKFB3-lactate signaling.
REFERENCE   4  (bases 1 to 2148)
  AUTHORS   Gao,L., Wang,C., Qin,B., Li,T., Xu,W., Lenahan,C., Ying,G., Li,J.,
            Zhao,T., Zhu,Y. and Chen,G.
  TITLE     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase Suppresses
            Neuronal Apoptosis by Increasing Glycolysis and 'cyclin-dependent
            kinase 1-Mediated Phosphorylation of p27 After Traumatic Spinal
            Cord Injury in Rats
  JOURNAL   Cell Transplant 29, 963689720950226 (2020)
   PUBMED   32841050
  REMARK    GeneRIF: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
            Suppresses Neuronal Apoptosis by Increasing Glycolysis and
            ''cyclin-dependent kinase 1-Mediated Phosphorylation of p27 After
            Traumatic Spinal Cord Injury in Rats.
REFERENCE   5  (bases 1 to 2148)
  AUTHORS   Wang,L., Cao,Y., Gorshkov,B., Zhou,Y., Yang,Q., Xu,J., Ma,Q.,
            Zhang,X., Wang,J., Mao,X., Zeng,X., Su,Y., Verin,A.D., Hong,M.,
            Liu,Z. and Huo,Y.
  TITLE     Ablation of endothelial Pfkfb3 protects mice from acute lung injury
            in LPS-induced endotoxemia
  JOURNAL   Pharmacol Res 146, 104292 (2019)
   PUBMED   31167111
REFERENCE   6  (bases 1 to 2148)
  AUTHORS   Goren,N., Manzano,A., Riera,L., Ambrosio,S., Ventura,F. and
            Bartrons,R.
  TITLE     6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase expression in
            rat brain during development
  JOURNAL   Brain Res Mol Brain Res 75 (1), 138-142 (2000)
   PUBMED   10648897
REFERENCE   7  (bases 1 to 2148)
  AUTHORS   Manzano,A., Perez,J.X., Nadal,M., Estivill,X., Lange,A. and
            Bartrons,R.
  TITLE     Cloning, expression and chromosomal localization of a human testis
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene
  JOURNAL   Gene 229 (1-2), 83-89 (1999)
   PUBMED   10095107
REFERENCE   8  (bases 1 to 2148)
  AUTHORS   Hirata,T., Kato,M., Okamura,N., Fukasawa,M. and Sakakibara,R.
  TITLE     Expression of human placental-type
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various
            cells and cell lines
  JOURNAL   Biochem Biophys Res Commun 242 (3), 680-684 (1998)
   PUBMED   9464277
REFERENCE   9  (bases 1 to 2148)
  AUTHORS   Watanabe,F., Sakai,A. and Furuya,E.
  TITLE     Novel isoforms of rat brain fructose 6-phosphate 2-kinase/fructose
            2,6-bisphosphatase are generated by tissue-specific alternative
            splicing
  JOURNAL   J Neurochem 69 (1), 1-9 (1997)
   PUBMED   9202288
REFERENCE   10 (bases 1 to 2148)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D87240.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D87240.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.2"
     gene            1..2148
                     /gene="Pfkfb3"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     3"
                     /db_xref="GeneID:117276"
                     /db_xref="RGD:619776"
     exon            1..481
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     CDS             406..2073
                     /gene="Pfkfb3"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="RB2K; 6PF-2-K/Fru-2,6-P2ASE brain-type isozyme;
                     PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 3"
                     /protein_id="NP_476476.1"
                     /db_xref="GeneID:117276"
                     /db_xref="RGD:619776"
                     /translation="MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLP
                     ARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQC
                     ALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTV
                     VASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDV
                     GRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGK
                     KFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTY
                     EEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVL
                     RCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEAVK
                     IQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNSVTPLASPEPTKKPRINSF
                     EEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLRTVCHIFSKFSPY"
     exon            482..607
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            608..704
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            705..771
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            772..846
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            847..903
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1028
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1236
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1383
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1488
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1618
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1681
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1746
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1833
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2007
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2030
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2083
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2148
                     /gene="Pfkfb3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaggctgacg cgccgcgcta ccggtcagat cttaaaaggc cgggcagtgc gtgtggcatc
       61 ccgacagaac aggagaggag caagtggcag cgcccggtgt tgggagcgtg gctgtcactg
      121 cgctggagca tccctgagct tctgaacaga gccgggtagt cagcagtgca gcagccgcaa
      181 ctccacgccg agccgagctc aatctgtctc gtgtctccgc tccggcgatc ctccgagact
      241 ccgcacaaag cacattttcc aaagcagcgc aagggagcgc ccagccgcgt gcaggcggac
      301 gcacccttcc tgtcctttgt tccggggggg atctgcagct gctcttctcc cccaactcgg
      361 agtcttccct ccaggggtgg caggtcgccg aatacagcaa cgaagatgcc tttggaactg
      421 acccagagcc gggtgcagaa gatctgggtg cctgtcgatc accgaccctc gttgcccaga
      481 tcctgtgggc cgaagctgac caactcccca acggtgattg tgatggtggg gctcccagcc
      541 cggggtaaga cttacatctc caagaagctg actcgctacc tcaactggat aggtgttcca
      601 acgaaagtgt tcaatgtggg agaataccga cgggaggctg tgaagcaata cagctcttac
      661 aacttcttcc gccctgacaa tgaggaggcc atgagagtcc gaaaacagtg tgccttagct
      721 gccctgagag acgtcaaaag ctacctgaca aaggaaggag gacagattgc agtttttgat
      781 gccaccaata cgacgagaga aaggaggcac atgatccttc attttgccaa agaaaatgac
      841 ttcaaggcat ttttcatcga gtccgtctgt gacgacccta cagttgtggc ctccaatatc
      901 atggaagtga aaatctccag cccggattac aaagactgca actccgcaga agccatggac
      961 gacttcatga agaggatcaa ctgctacgaa gccagctacc agcctcttga ccctgataaa
     1021 tgtgacaggg acctgtcatt catcaaagtg attgacgttg gccggaggtt cttggtgaac
     1081 agagtgcagg accacatcca gagccgaatc gtgtattacc tgatgaacat ccacgtgcag
     1141 ccccggacca tctacctgtg tcggcacggc gagaatgagt acaacgtgca gggcaagatt
     1201 gggggcgact caggcctgtc cagcagaggc aagaagttcg ccaatgccct gagcaagttc
     1261 gttgaagagc agaatctgaa ggacctccgc gtgtggacca gtcagctgaa aagtaccatc
     1321 cagaccgcgg aggccctaag gctcccctac gagcagtgga aggcgctcaa tgagattgat
     1381 gctggtgtgt gtgaggaact aacctatgag gagatcagag acacctaccc tgaggagtat
     1441 gcactgcgtg agcaagacaa atactactac cgctatccca caggcgagtc ctaccaggat
     1501 cttgtccagc gcctggagcc tgtgatcatg gaattagagc gccaagagaa cgtattggtc
     1561 atctgtcacc aggctgtcct gcgctgccta ctagcctact tccttgacaa gagtgcagag
     1621 gagatgccat acctgaaatg cccacttcac acagtcctga aactgacgcc cgtggcgtat
     1681 gggtgccgtg tggagtccat ttacttgaat gtagaatcag tgagcacgca tcgggagagg
     1741 tcagaggctg tcaaaataca acactttgcc agtgtagtca gaccttcctc ttacaccgaa
     1801 cttgactttc tgtctgtgga gtccgcaaaa caggatgcaa agaagggacc taaccctctc
     1861 atgagacgca atagtgtcac cccactagcc agccccgagc ccaccaaaaa gcctcgcatc
     1921 aacagctttg aggagcatgt ggcttccact tctgctgccc tgccaagctg cctgcccccg
     1981 gaagtgccca cgcagctgcc cggacaacct ttgctaggga aagcctgtct acgaactgtc
     2041 tgtcacattt tttcaaagtt ttctccttac taacctggga aagaacatga ggagcccccg
     2101 gagcggtgcc gaatcttccc agaagcactg atgctgaagt aggggttc
//
