LOCUS       NM_001033965            3824 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus
            6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2),
            transcript variant 1, mRNA.
ACCESSION   NM_001033965
VERSION     NM_001033965.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Novellasdemunt,L., Tato,I., Navarro-Sabate,A., Ruiz-Meana,M.,
            Mendez-Lucas,A., Perales,J.C., Garcia-Dorado,D., Ventura,F.,
            Bartrons,R. and Rosa,J.L.
  TITLE     Akt-dependent activation of the heart
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2)
            isoenzyme by amino acids
  JOURNAL   J Biol Chem 288 (15), 10640-10651 (2013)
   PUBMED   23457334
  REMARK    GeneRIF: amino acids stimulate Fru-2,6-P2 synthesis by
            Akt-dependent PFKFB2 phosphorylation and activation and show how
            signaling and metabolism are inextricably linked.
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Minchenko,D.O., Mykhalchenko,V.G., Tsuchihara,K., Kanehara,S.,
            Yavorovsky,O.P., Zavgorodny,I.V., Paustovsky,Y.O.,
            Komisarenko,S.V., Esumi,H. and Minchenko,O.H.
  TITLE     Alternative splice variants of rat 6-phosphofructo-2-kinase/
            fructose-2,6-bisphosphatase-4 mRNA
  JOURNAL   Ukr Biokhim Zh (1999) 80 (4), 66-73 (2008)
   PUBMED   19140452
REFERENCE   3  (bases 1 to 3824)
  AUTHORS   Arden,C., Hampson,L.J., Huang,G.C., Shaw,J.A., Aldibbiat,A.,
            Holliman,G., Manas,D., Khan,S., Lange,A.J. and Agius,L.
  TITLE     A role for PFK-2/FBPase-2, as distinct from fructose
            2,6-bisphosphate, in regulation of insulin secretion in pancreatic
            beta-cells
  JOURNAL   Biochem J 411 (1), 41-51 (2008)
   PUBMED   18039179
  REMARK    GeneRIF: Rat islets expressed PFKFB2 lacking the C-terminal
            phosphorylation sites. PFK-2/FBPase-2 protein rather than its
            product fructose 2,6-P(2) is the over-riding determinant of
            glucose-induced insulin secretion through regulation of glucokinase
            activity
REFERENCE   4  (bases 1 to 3824)
  AUTHORS   Mukhtar,M.H., Payne,V.A., Arden,C., Harbottle,A., Khan,S.,
            Lange,A.J. and Agius,L.
  TITLE     Inhibition of glucokinase translocation by AMP-activated protein
            kinase is associated with phosphorylation of both GKRP and
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 294 (3), R766-R774 (2008)
   PUBMED   18199594
  REMARK    GeneRIF: mechanisms downstream of AMP-activated protein kinase
            activation, involving phosphorylation of
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and GKRP are
            involved in the ATP-independent inhibition of glucose-induced
            glucokinase translocation
REFERENCE   5  (bases 1 to 3824)
  AUTHORS   Mykhalchenko,V.G., Minchenko,D.O., Tsuchihara,K., Moenner,M.,
            Komisarenko,S.V., Bikfalvi,A., Esumi,H. and Minchenko,O.H.
  TITLE     Expression of mouse
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 mRNA
            alternative splice variants in hypoxia
  JOURNAL   Ukr Biokhim Zh (1999) 80 (1), 19-25 (2008)
   PUBMED   18710022
REFERENCE   6  (bases 1 to 3824)
  AUTHORS   Chikri,M. and Rousseau,G.G.
  TITLE     Rat gene coding for heart
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase:
            characterization of an unusual promoter region and identification
            of four mRNAs
  JOURNAL   Biochemistry 34 (27), 8876-8884 (1995)
   PUBMED   7612629
REFERENCE   7  (bases 1 to 3824)
  AUTHORS   Watanabe,F., Sakai,A., Furuya,E. and Uyeda,K.
  TITLE     Molecular cloning and tissue specific expression of fructose
            6-phosphate,2-kinase:fructose 2,6-bisphosphatase of rat brain
  JOURNAL   Biochem Biophys Res Commun 198 (1), 335-340 (1994)
   PUBMED   8292038
REFERENCE   8  (bases 1 to 3824)
  AUTHORS   Montalbetti,L., Marinoni,P., Lampertico,P., Borri,C., Candiani,A.
            and Brambilla-Pisoni,G.
  TITLE     [Primary lymphoma of the kidney. Description of a case and brief
            review of the literature]
  JOURNAL   Minerva Urol Nefrol 44 (4), 249-251 (1992)
   PUBMED   1299004
  REMARK    Review article
REFERENCE   9  (bases 1 to 3824)
  AUTHORS   Darville,M.I., Chikri,M., Lebeau,E., Hue,L. and Rousseau,G.G.
  TITLE     A rat gene encoding heart
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   FEBS Lett 288 (1-2), 91-94 (1991)
   PUBMED   1652483
REFERENCE   10 (bases 1 to 3824)
  AUTHORS   Algaier,J. and Uyeda,K.
  TITLE     Molecular cloning, sequence analysis, and expression of a human
            liver cDNA coding for
            fructose-6-P,2-kinase:fructose-2,6-bisphosphatase
  JOURNAL   Biochem Biophys Res Commun 153 (1), 328-333 (1988)
   PUBMED   2837207
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On May 5, 2015 this sequence version replaced NM_001033965.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB040531.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-169               JAXUCZ010000013.1  44726803-44726971   c
            170-283             JAXUCZ010000013.1  44724534-44724647   c
            284-409             JAXUCZ010000013.1  44718263-44718388   c
            410-506             JAXUCZ010000013.1  44717687-44717783   c
            507-573             JAXUCZ010000013.1  44717257-44717323   c
            574-648             JAXUCZ010000013.1  44717046-44717120   c
            649-705             JAXUCZ010000013.1  44716626-44716682   c
            706-830             JAXUCZ010000013.1  44715574-44715698   c
            831-1038            JAXUCZ010000013.1  44712412-44712619   c
            1039-1185           JAXUCZ010000013.1  44711888-44712034   c
            1186-1290           JAXUCZ010000013.1  44710981-44711085   c
            1291-1420           JAXUCZ010000013.1  44710188-44710317   c
            1421-1483           JAXUCZ010000013.1  44709435-44709497   c
            1484-1548           JAXUCZ010000013.1  44708936-44709000   c
            1549-1728           JAXUCZ010000013.1  44708183-44708362   c
            1729-1797           JAXUCZ010000013.1  44707500-44707568   c
            1798-3824           JAXUCZ010000013.1  44699744-44701770   c
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     gene            1..3824
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     2"
                     /db_xref="GeneID:24640"
                     /db_xref="RGD:3309"
     exon            1..169
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            170..283
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..1863
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform a is encoded by transcript variant 1;
                     fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase;
                     PFK-2/FBPase-2; 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme;
                     PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase 2;
                     6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
                     (heart)"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 2 isoform a"
                     /protein_id="NP_001029137.1"
                     /db_xref="GeneID:24640"
                     /db_xref="RGD:3309"
                     /translation="MSENSTFSTEDSSSSSYKPHASNLRRAGKKCSWASYMTNSPTLI
                     VMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRHDNEEAM
                     KIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRDMILNFAKQNAFKVFFVESV
                     CDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDNYDKDLSF
                     IKVMNVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSG
                     LSLRGKQFAQALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGV
                     CEEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVI
                     SHQAVMRCLLAYFLDKGADELPYLRCPLHIIFKLTPVAYGCKVETITLNVEAVDTHRD
                     KPTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRALDMQEGAD
                     QPKTQVQQGSAQATEHLQKALEFANGHREVENVLAKHRRPSMASLTLLS"
     exon            284..409
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            410..506
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            507..573
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            574..648
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            649..705
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            706..830
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1038
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1185
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1290
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1420
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1483
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1548
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1728
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1797
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..3824
                     /gene="Pfkfb2"
                     /gene_synonym="PFK-2; RH2K"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcctcactcg ggtggtggca gtgttcaaga ggactaactc ttgtcatccg gattccagtt
       61 cgattcctcg gatccaatta ggaagacact ccatcatgcg attggagtcc tcgtcgtggc
      121 ccagagatag aagacggacg gggaagggga gacctaacca gatagctagg tgcctcctga
      181 agaactacca tgtctgagaa tagtacattt tccacagaag acagcagcag cagcagctat
      241 aaaccccacg cctcaaacct ccgaagggca gggaagaaat gctcatgggc ttcctacatg
      301 accaactccc caacactcat tgttatgatt ggcttgccag cccggggtaa gacttatgtg
      361 tccaagaaat taacacgcta cctgaactgg attggagtac ctaccaaagt gtttaatctt
      421 ggtgtatatc gacgggaagc agtcaagtcc tataagtcct atgacttctt tcgacatgac
      481 aatgaagagg ccatgaagat ccgcaaacag tgtgccctgg tggcactgga agatgtgaag
      541 gcctacttta ctgaagagag tgggcagatc gcggtgtttg atgccaccaa taccactcgg
      601 gagaggaggg acatgatttt gaactttgcc aagcagaatg ccttcaaggt attctttgtg
      661 gaatctgtgt gtgatgatcc tgatgtcatt gctgccaata ttctggaggt aaaagtgtca
      721 agccctgact accccgaaag gaatagggag aatgtgatgg aggacttcct gaagagaatt
      781 gagtgctaca aggtcactta ccagcccctt gacccagaca actatgataa ggacctctcg
      841 ttcataaagg tgatgaatgt aggccagagg tttctggtca acagagttca ggactacatc
      901 cagagtaaga ttgtctacta cctgatgaac atccatgtcc atcctcgcac catctatctg
      961 tgccggcacg gagagagcga attcaatctt ttgggaaaga ttgggggtga ctctggcctt
     1021 tcgttgcgag gaaagcagtt tgctcaggct ctgaagaagt ttctggagga acaggagatc
     1081 caggacctca aagtgtggac aagccagttg aagaggacaa ttcagactgc tgagtctctt
     1141 ggggtgacct atgagcaatg gaagatccta aatgagattg atgctggcgt gtgtgaggag
     1201 atgacttatt cggagatcga acaacggtat ccagaggaat ttgcacttcg agatcaagag
     1261 aagtatctgt atcgatatcc tggtggggag tcataccagg acctggtgca gcggctggag
     1321 cctgtgatca tggagctgga gcggcaaggc aacgtcctcg ttatctctca ccaggctgtc
     1381 atgcgctgcc tcctggccta cttcttggat aaaggcgcag atgagttgcc gtacttgagg
     1441 tgccccctcc acatcatctt caaacttact cctgtggcct atggttgcaa agtggaaaca
     1501 attacactga atgtggaagc tgtggacaca catcgtgaca agccaactca caacttcccc
     1561 aagagccaaa cccctgtaag gatgagaagg aacagcttta cgcctctgtc cagttcgaac
     1621 acaataaggc gtccaagaaa ttacagtgtt gggagccgac ccctcaagcc cctcagccct
     1681 ctccgtgccc tggatatgca agaaggggcg gaccagccga agacccaagt ccagcaaggg
     1741 tcagcccagg ctacagaaca tctacagaaa gccctggagt ttgcaaatgg acatagggaa
     1801 gtggagaatg tgctggctaa gcatagacgc ccctcaatgg catccctcac tttgctctcc
     1861 tgatgtgagg ctgaggccag acctctccga ggaactggat ctaccaaaaa gcttggcatg
     1921 ccagcatccc tgaggctaga acaggaagtt aagtgggaac gtctcatttg aggctaccaa
     1981 cagtaaggcg tgggaaacct tcactgcatt tctacccctc atgcccaaca ggaacattac
     2041 tacattgtgc ctcaaaaagt cgtcaacagc ctgagcaagt tagttcttgt ctcttttgaa
     2101 ccaaatttgc aaatgccatt ctaacagagg cattcagcat tctacctgtg gccgactgac
     2161 tgcatcctta gctggagatt tgcctaatgt gtgaatgcac tgcttcagaa ttggtttcta
     2221 gatgagggtg aacttcagga agccttttct gctccatgag agctgctctt ttcaccggac
     2281 ggaggagagc agggtgaagt gcagtctgag tgctagtggt cgcctcctgc agtgtgggag
     2341 atgagacaga gcaccaggtg aatggagggc gtcggaaact aagctcgcaa gtgctcttct
     2401 ctggtagcag aagagtagtg tccatggttc ccttcccaca tagagaagtt ttccttgttc
     2461 catatccttt ggtgcagggt ctggctgctg gacttcccca aagggaccaa cccttacatt
     2521 ctctcgtagc tactgttttc tctctgaggc ttgtaactca cacagataga caggactgaa
     2581 ccagtgcctc ctgaggagtt gggactgaag ggaccatctc caacccatga agaaatagag
     2641 atggcttctt tttttttttt aaatttcaaa ataggcagaa gcagccatgc ccctgtgtct
     2701 agttgatgtc gtctgtggaa agctgtgcct gtgcctgcgg gagcagtggg cctatgctcg
     2761 gtgcctgttg acgctgtcac tcctgtgatc agcaggtctc ccacagcacg tggcagatgc
     2821 ggcaaagccg tggggcatct atgagtgagt gtctccttca gaagaatccg attgctgtcc
     2881 gcaggtcact gagtgtctga tgcatactca ctattaccat gagggcaccc ctagtgttac
     2941 ttctgaagaa gaatgcagaa gccacaaagt gtactgtacc tcaccagtgt tactgtggtt
     3001 ctttgttcca tttcactgaa gctgcacact tgttgggcag aaagataagt agcaagaaaa
     3061 gtaataaaat gtgctttttc taaatgttct ttcatactgt agacaatact ggagactgtt
     3121 acccaaggag gatgtgatga gctgctggca gttcactgac tcccaggctg ctgcttgctt
     3181 tctatgtatg catgtccctg gagagaggct ccctcctccc tctgcctggg acaggcgttt
     3241 ccccgtggct gatgcagcat actgtactac agggtttttt ttcttgtggt ataggatgct
     3301 ctctgcattg aagtgcaccc ctttctgatg atcagatggt atttttatga agctatttat
     3361 tttgtgtgag tttgcatgta tttatcagaa tgtcatttct gttctcagtg ttctgctagc
     3421 attgtaggcg gcttgaacgc tgagcctaat gtctatagta ttaccttaag ttaaatgtgt
     3481 agagcgtaaa tgtttgtata tgtcaaagaa tggtaactgt tatgactgta gatttttcaa
     3541 ttttttttct tattgatttt attataaacc aagaaatgta tttctctgga aaacaggttc
     3601 tctcttagat aagtaaaacc atgctaaaat atctcaacaa catatactgc ttttgtaata
     3661 agaaaataaa gttattttta attaaaaggt acaatgaaat tttcaattcg catgcaaaaa
     3721 cacatcattt gatacattac atatgcacaa aatattaaca ttttccaaaa tgtacttgaa
     3781 agtttattaa ggatgtttga aattattaaa tggtatctgt ttcc
//
