LOCUS       NM_012621               1830 bp    mRNA    linear   ROD 28-DEC-2024
DEFINITION  Rattus norvegicus
            6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1),
            transcript variant 1, mRNA.
ACCESSION   NM_012621
VERSION     NM_012621.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Gibb,A.A., Lorkiewicz,P.K., Zheng,Y.T., Zhang,X., Bhatnagar,A.,
            Jones,S.P. and Hill,B.G.
  TITLE     Integration of flux measurements to resolve changes in anabolic and
            catabolic metabolism in cardiac myocytes
  JOURNAL   Biochem J 474 (16), 2785-2801 (2017)
   PUBMED   28706006
  REMARK    GeneRIF: Data suggest that a major portion of intracellular glucose
            in cardiac myocytes is apportioned for ancillary biosynthetic
            reactions; PFKFB1 coordinates activities of pentose phosphate,
            hexosamine biosynthetic, and fatty acid/glycerolipid synthesis
            pathways by directly modulating glycolytic intermediate entry into
            auxiliary glucose metabolism pathways and by indirectly regulating
            mitochondrial cataplerosis.
            Erratum:[Biochem J. 2017 Dec 14;474(24):4271-4272. doi:
            10.1042/BCJ20170474_COR. PMID: 29242383]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Cosin-Roger,J., Vernia,S., Alvarez,M.S., Cucarella,C., Bosca,L.,
            Martin-Sanz,P., Fernandez-Alvarez,A.J. and Casado,M.
  TITLE     Identification of a novel Pfkfb1 mRNA variant in rat fetal liver
  JOURNAL   Biochem Biophys Res Commun 431 (1), 36-40 (2013)
   PUBMED   23291237
  REMARK    GeneRIF: a new mRNA variant which are transcribed from a new Pfkfb1
            gene promoter in fetal rat liver was characterized.
REFERENCE   3  (bases 1 to 1830)
  AUTHORS   Langer,S., Okar,D.A., Schultz,J., Lenzen,S. and Baltrusch,S.
  TITLE     Dimer interface rearrangement of the
            6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver
            isoenzyme by cAMP-dependent Ser-32 phosphorylation
  JOURNAL   FEBS Lett 586 (10), 1419-1425 (2012)
   PUBMED   22668829
  REMARK    GeneRIF: flexible kinase interaction mode exists, suggesting dimer
            conformation mediated coupling of hormonal and posttranslational
            enzyme regulation to the metabolic response in liver
REFERENCE   4  (bases 1 to 1830)
  AUTHORS   Manes,N.P. and El-Maghrabi,M.R.
  TITLE     The kinase activity of human brain
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated
            via inhibition by phosphoenolpyruvate
  JOURNAL   Arch Biochem Biophys 438 (2), 125-136 (2005)
   PUBMED   15896703
REFERENCE   5  (bases 1 to 1830)
  AUTHORS   Rider,M.H., Bertrand,L., Vertommen,D., Michels,P.A., Rousseau,G.G.
            and Hue,L.
  TITLE     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head
            with a bifunctional enzyme that controls glycolysis
  JOURNAL   Biochem J 381 (Pt 3), 561-579 (2004)
   PUBMED   15170386
  REMARK    Review article
REFERENCE   6  (bases 1 to 1830)
  AUTHORS   Crepin,K.M., De Cloedt,M., Vertommen,D., Foret,D., Michel,A.,
            Rider,M.H., Rousseau,G.G. and Hue,L.
  TITLE     Molecular forms of
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase expressed in
            rat skeletal muscle
  JOURNAL   J Biol Chem 267 (30), 21698-21704 (1992)
   PUBMED   1328243
REFERENCE   7  (bases 1 to 1830)
  AUTHORS   Darville,M.I., Antoine,I.V. and Rousseau,G.G.
  TITLE     Characterization of an enhancer upstream from the muscle-type
            promoter of a gene encoding
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   Nucleic Acids Res 20 (14), 3575-3583 (1992)
   PUBMED   1322527
REFERENCE   8  (bases 1 to 1830)
  AUTHORS   Rosa,J.L., Tauler,A., Lange,A.J., Pilkis,S.J. and Bartrons,R.
  TITLE     Transcriptional and posttranscriptional regulation of
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase during liver
            regeneration
  JOURNAL   Proc Natl Acad Sci U S A 89 (9), 3746-3750 (1992)
   PUBMED   1315037
REFERENCE   9  (bases 1 to 1830)
  AUTHORS   Kurland,I.J., el-Maghrabi,M.R., Correia,J.J. and Pilkis,S.J.
  TITLE     Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
            Properties of phospho- and dephospho- forms and of two mutants in
            which Ser32 has been changed by site-directed mutagenesis
  JOURNAL   J Biol Chem 267 (7), 4416-4423 (1992)
   PUBMED   1339450
REFERENCE   10 (bases 1 to 1830)
  AUTHORS   Kitamura,K., Uyeda,K., Hartman,F.C., Kangawa,K. and Matsuo,H.
  TITLE     Catalytic site of rat liver and bovine heart
            fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase.
            Identification of fructose 6-phosphate binding site
  JOURNAL   J Biol Chem 264 (11), 6344-6348 (1989)
   PUBMED   2539378
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            Y00702.1.
            
            [WARNING] On Jan 4, 2025 this sequence was replaced by NM_012621.5.
            
            On Oct 14, 2007 this sequence version replaced NM_012621.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y00702.1, J04197.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq12"
     gene            1..1830
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     1"
                     /db_xref="GeneID:24638"
                     /db_xref="RGD:3307"
     exon            1..269
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1588
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform 1 is encoded by transcript variant 1;
                     6PF-2-K/Fru-2,6-P2ASE liver isozyme; PFK/FBPase 1;
                     6PF-2-K/Fru-2,6-P2ase 1;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1;
                     6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase;
                     PFK2/FBPase2; 6-PF2-K/Fru-2,6-P2ase"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 1 isoform 1"
                     /protein_id="NP_036753.4"
                     /db_xref="GeneID:24638"
                     /db_xref="RGD:3307"
                     /translation="MSREMGELTQTRLQKIWIPHSSSSSVLQRRRGSSIPQFTNSPTM
                     VIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQ
                     LIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESI
                     CNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYI
                     KIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGL
                     SARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVC
                     EEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVIC
                     HQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDK
                     PENVDITREAEEALDTVPAHY"
     exon            270..395
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            396..489
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            490..556
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            557..631
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            632..688
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            689..810
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            811..1018
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1165
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1270
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1400
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1463
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1528
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1830
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1830
                     /gene="Pfkfb1"
                     /gene_synonym="Pfkfb01; PFRX"
                     /note="polyA site"
ORIGIN      
        1 aaaagaggga gctggagata atctgtgaga ggctccgaag cccagtacat ccccctgact
       61 cagccctgcc cacctgctgc tgtgactaca gcaacagaca agacagctag gaagatagga
      121 agtgaggtcc aataccttgt gggcagtggt gtcattagct gcaacgccta agatgtctcg
      181 agagatggga gaactcactc aaaccaggtt acagaagatc tggattccac acagcagcag
      241 tagcagcgtg ctgcaacggc gaaggggctc ctccatacca cagttcacta attctcccac
      301 gatggtgatc atggtgggtt taccagctcg aggcaagacc tacatctcta cgaagctcac
      361 acgctatctc aactggatag gaacaccaac taaagtgttt aatttaggtc agtatcgacg
      421 agaggcagtg agttacagga actatgaatt ctttcgccca gacaacacag aggcccagct
      481 tatcaggaag cagtgtgctc tagcagccct aaaggatgtc cataagtatc tcagccgcga
      541 ggaaggtcat gttgcggttt ttgatgccac caacactacc agagaacgac gatcgttgat
      601 tctacagttt gctaaggaac atggttataa ggtcttcttt attgagtcta tttgtaatga
      661 ccccgaaatc attgcagaaa acatcaagca agtgaaactt ggtagtcctg attacataga
      721 ctgtgaccaa gaaaaggttt tggaagactt tctaaagaga atagagtgct atgagatcaa
      781 ctaccaacct ttggatgagg aattggacag ccacctgtcc tacatcaaga tcttcgacgt
      841 gggcacacgc tacatggtaa atcgagtgca ggaccacgtt cagagccgta cagcctacta
      901 cctcatgaac atccatgtca cacctcgatc tatctaccta tgccgccatg gtgagagtga
      961 actcaacctt agaggccgca ttggaggtga ctctggcctc tcagctcggg gcaagcagta
     1021 tgcctatgca ctagccaact tcatccggtc tcaaggcatc agctccctga aagtatggac
     1081 tagccacatg aagaggacca ttcagaccgc tgaagcccta ggtgtcccct atgaacagtg
     1141 gaaggccctg aatgagattg atgcgggtgt ctgtgaagag atgacctatg aagaaattca
     1201 ggaacactac cctgaggaat ttgcactacg ggaccaggat aaatatcgtt accgctatcc
     1261 caagggagag tcctatgagg atctggttca gcgtcttgaa ccagttataa tggagctaga
     1321 acggcaagaa aatgtactgg tgatctgtca ccaggctgtc atgcggtgcc tcctggcata
     1381 cttcctggat aaaagttcag atgagctgcc ctatctcaag tgtcctctgc atactgtgct
     1441 caaactcaca cctgtggctt atggctgcag agtggagtcc atctacctga atgtggaggc
     1501 tgtgaacaca caccgggaca agcctgagaa tgtggacatc acccgtgaag ctgaggaagc
     1561 cttggacact gtacctgccc attactgagc ccttttcaag tgatcagatt gtctctgttc
     1621 tcaccccctt cctcctgtag gagctgctgc ccttgttctc cttaagcaga ctctggctat
     1681 ggcctgagag tgccctacct ccagtgaaga agtccgtagc agctcccaaa caggtctcaa
     1741 ttcctagcca caactaaggc acctacctag ttgtggataa agttcctttt aaatattctc
     1801 tgataaataa agatgtttat ttctgcctat
//
