LOCUS       NM_001393826            8404 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus peroxisomal biogenesis factor 5-like (Pex5l),
            transcript variant 5, mRNA.
ACCESSION   NM_001393826
VERSION     NM_001393826.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Foote KM, Lyman KA, Han Y, Michailidis IE, Heuermann RJ, Mandikian
            D, Trimmer JS, Swanson GT and Chetkovich DM.
  TITLE     Phosphorylation of the HCN channel auxiliary subunit TRIP8b is
            altered in an animal model of temporal lobe epilepsy and modulates
            channel function
  JOURNAL   J Biol Chem 294 (43), 15743-15758 (2019)
   PUBMED   31492750
  REMARK    GeneRIF: loss of TRIP8b phosphorylation may affect HCN channel
            properties during epileptogenesis.
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   Wilkars W, Wollberg J, Mohr E, Han M, Chetkovich DM, Bahring R and
            Bender RA.
  TITLE     Nedd4-2 regulates surface expression and may affect N-glycosylation
            of hyperpolarization-activated cyclic nucleotide-gated (HCN)-1
            channels
  JOURNAL   FASEB J 28 (5), 2177-2190 (2014)
   PUBMED   24451387
  REMARK    GeneRIF: Nedd4-2 plays an important role in the regulation of HCN1
            trafficking and may compete with TRIP8b(1a-4) in this process
REFERENCE   3  (bases 1 to 8404)
  AUTHORS   Ghai R, Bugarcic A, Liu H, Norwood SJ, Skeldal S, Coulson EJ, Li
            SS, Teasdale RD and Collins BM.
  TITLE     Structural basis for endosomal trafficking of diverse transmembrane
            cargos by PX-FERM proteins
  JOURNAL   Proc Natl Acad Sci U S A 110 (8), E643-E652 (2013)
   PUBMED   23382219
REFERENCE   4  (bases 1 to 8404)
  AUTHORS   Wilkars W, Liu Z, Lewis AS, Stoub TR, Ramos EM, Brandt N, Nicholson
            DA, Chetkovich DM and Bender RA.
  TITLE     Regulation of axonal HCN1 trafficking in perforant path involves
            expression of specific TRIP8b isoforms
  JOURNAL   PLoS One 7 (2), e32181 (2012)
   PUBMED   22363812
  REMARK    GeneRIF: TRIP8b isoforms are important regulators of HCN1
            trafficking in entorhinal neurons.
REFERENCE   5  (bases 1 to 8404)
  AUTHORS   Sheets PL, Suter BA, Kiritani T, Chan CS, Surmeier DJ and Shepherd
            GM.
  TITLE     Corticospinal-specific HCN expression in mouse motor cortex:
            I(h)-dependent synaptic integration as a candidate microcircuit
            mechanism involved in motor control
  JOURNAL   J Neurophysiol 106 (5), 2216-2231 (2011)
   PUBMED   21795621
  REMARK    GeneRIF: Hyperpolarization current expression was high in
            corticospinal neurons and low in corticostriatal and
            corticocortical neurons, a pattern mirrored by mRNA levels for HCN1
            and Trip8b subunits.
REFERENCE   6  (bases 1 to 8404)
  AUTHORS   Popova NV, Plotnikov AN, Ziganshin RKh, Deyev IE and Petrenko AG.
  TITLE     Analysis of proteins interacting with TRIP8b adapter
  JOURNAL   Biochemistry (Mosc) 73 (6), 644-651 (2008)
   PUBMED   18620529
  REMARK    GeneRIF: A role of TRIP8b in receptor-mediated endocytosis.
REFERENCE   7  (bases 1 to 8404)
  AUTHORS   Fransen M, Amery L, Hartig A, Brees C, Rabijns A, Mannaerts GP and
            Van Veldhoven PP.
  TITLE     Comparison of the PTS1- and Rab8b-binding properties of Pex5p and
            Pex5Rp/TRIP8b
  JOURNAL   Biochim Biophys Acta 1783 (5), 864-873 (2008)
   PUBMED   18346465
REFERENCE   8  (bases 1 to 8404)
  AUTHORS   Santoro B, Wainger BJ and Siegelbaum SA.
  TITLE     Regulation of HCN channel surface expression by a novel C-terminal
            protein-protein interaction
  JOURNAL   J Neurosci 24 (47), 10750-10762 (2004)
   PUBMED   15564593
REFERENCE   9  (bases 1 to 8404)
  AUTHORS   Amery L, Sano H, Mannaerts GP, Snider J, Van Looy J, Fransen M and
            Van Veldhoven PP.
  TITLE     Identification of PEX5p-related novel peroxisome-targeting signal 1
            (PTS1)-binding proteins in mammals
  JOURNAL   Biochem J 357 (Pt 3), 635-646 (2001)
   PUBMED   11463335
REFERENCE   10 (bases 1 to 8404)
  AUTHORS   Chen S, Liang MC, Chia JN, Ngsee JK and Ting AE.
  TITLE     Rab8b and its interacting partner TRIP8b are involved in regulated
            secretion in AtT20 cells
  JOURNAL   J Biol Chem 276 (16), 13209-13216 (2001)
   PUBMED   11278749
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000067.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001393826.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               JACYVU010000067.1  30975584-30975987   c
            405-476             JACYVU010000067.1  30916349-30916420   c
            477-581             JACYVU010000067.1  30841896-30842000   c
            582-693             JACYVU010000067.1  30832357-30832468   c
            694-888             JACYVU010000067.1  30824580-30824774   c
            889-1012            JACYVU010000067.1  30823362-30823485   c
            1013-1109           JACYVU010000067.1  30822089-30822185   c
            1110-1205           JACYVU010000067.1  30810820-30810915   c
            1206-1322           JACYVU010000067.1  30775956-30776072   c
            1323-1466           JACYVU010000067.1  30774590-30774733   c
            1467-1537           JACYVU010000067.1  30773647-30773717   c
            1538-1735           JACYVU010000067.1  30772102-30772299   c
            1736-1901           JACYVU010000067.1  30770789-30770954   c
            1902-2059           JACYVU010000067.1  30770279-30770436   c
            2060-8404           JACYVU010000067.1  30762490-30768834   c
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     gene            1..8404
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /note="peroxisomal biogenesis factor 5-like"
                     /db_xref="GeneID:286937"
                     /db_xref="RGD:708407"
     exon            1..404
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     CDS             384..2264
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /note="isoform 5 is encoded by transcript variant 5;
                     PEX5-related protein; pex5Rp; PEX5-like protein;
                     peroxin-5-related protein; peroxin 2; TPR-containing
                     Rab8b-interacting protein; tetratricopeptide
                     repeat-containing Rab8b-interacting protein"
                     /codon_start=1
                     /product="PEX5-related protein isoform 5"
                     /protein_id="NP_001380755.1"
                     /db_xref="GeneID:286937"
                     /db_xref="RGD:708407"
                     /translation="MYQGHMQKSKEQGYGKLSSDEDLEIIVDQKQGKGSRAADKAVAM
                     VMKEIPREESAEEKPLLTMTSQLVNEQQESRPLLSPSIDDFLCETKSEAIAKPVTSNT
                     AVLTTGLDLLDLSEPVSQTQTKAKKSESSSKSSSLKKKADGSDLISADAEQRAQALRG
                     PETSSLDLDIQTQLEKWDDVKFHGDRTSKGHLMAERKSCSSRAGSKELLWSSEHRSQP
                     ELSTGKSALNSESASELELVAPAQARLTKEHRWGSALLSRNHSLEEEFERAKAAVESD
                     TEFWDKMQAEWEEMARRNWISENQEAQNQVTVSASEKGYYFHTENPFKDWPGAFEEGL
                     KRLKEGDLPVTILFMEAAILQDPGDAEAWQFLGITQAENENEQAAIVALQRCLELQPN
                     NLKALMALAVSYTNTSHQQDACEALKNWIKQNPKYKYLVKNKKGSPGLTRRMSKSPVD
                     SSVLEGVKDLYLEAAHQNGDMIDPDLQTGLGVLFHLSGEFNRAIDAFNAALTVRPEDY
                     SLWNRLGATLANGDRSEEAVEAYTRALEIQPGFIRSRYNLGISCINLGAYREAVSNFL
                     TALSLQRKSRNQQQVPHPAISGNIWAALRIALSLMDQPELFQAANLGDLDVLLRAFNL
                     DP"
     exon            405..476
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            477..581
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            582..693
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            694..888
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1012
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1109
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1205
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1322
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1466
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1537
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1735
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1901
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2059
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..8404
                     /gene="Pex5l"
                     /gene_synonym="Pex2; TRIP8b; Trjip8b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcgcgcgcc ctgcgctcgg cagctgcggc ggcggctggc gcgggctggc gtccggacgg
       61 aggcgggagg ggacgctgca gcctcgtgaa aggcgaggag agtggggcac aggaccggcg
      121 agttggagag gcgcggcgag cggcactgcg cccgcggaga gcccggcccc gcgtccccgt
      181 gcccgcgcgc gctcagcacc cgcgtggagg acagctccgc agcgcgccgc tcgcccagcc
      241 gccctctcgc ccgcctgctt ctgtaaccca cagccggggc gcccggcgga gagcccgcgc
      301 ccgctgcccg acctgcaact ccctacgagc cagctcggga aagcagtcat cggcctcggg
      361 agccctgacg acgctggagc agaatgtacc agggacacat gcagaaaagt aaagaacaag
      421 gctatggaaa gctaagcagt gatgaagacc tcgaaataat tgttgatcag aagcagggaa
      481 aaggctctag ggctgcagat aaggctgttg ccatggtgat gaaggagata ccgagggagg
      541 agtctgcaga agaaaagccc ctccttacta tgacatcaca gctggtgaat gagcaacaag
      601 aaagcagacc tctcctgagc ccctccatcg acgacttcct ctgtgaaacc aaatccgagg
      661 ccattgcaaa gccagtgacg tccaacacag ctgtgttgac cactggcttg gatctccttg
      721 acctgagtga acccgtctct caaacccaaa ccaaagccaa gaaatcagag tcctcatcga
      781 agagttcatc cctcaagaag aaagctgatg gctctgacct catcagtgca gatgctgagc
      841 agagagcaca ggctctccga gggccagaga catcatcctt agatttagat attcaaacac
      901 aactggaaaa atgggatgat gtcaagtttc atggtgaccg aacaagcaag gggcacctca
      961 tggcagagag gaagtcatgc tcttccagag ccggatccaa agagcttctg tggtcctcag
     1021 aacatagatc tcagccagag ctgagtaccg ggaaaagtgc cctcaactcc gagtcggctt
     1081 ctgagttgga acttgtggct ccagcacagg ctcggctgac caaagagcat cgctggggaa
     1141 gcgccctgct ctccaggaac cattccttag aagaggagtt tgagagggca aaagcagcag
     1201 tagagtcaga tacggagttc tgggataaga tgcaggcaga atgggaagaa atggcccgga
     1261 ggaactggat atcggagaac caggaagctc agaaccaagt caccgtctca gccagcgaga
     1321 agggttatta ctttcacact gaaaatccct tcaaggactg gcctggagca tttgaagaag
     1381 gcttaaaaag actgaaggaa ggggacctgc ccgtcaccat cctgttcatg gaagcagcga
     1441 tccttcagga ccctggagac gctgaggcat ggcagtttct tggtataacc caggccgaga
     1501 atgaaaacga acaggcagcc attgtcgccc tccagaggtg cctggagttg cagcccaaca
     1561 acttgaaggc tctgatggcc ttggctgtga gttacaccaa taccagccac cagcaggatg
     1621 cttgtgaggc gctgaagaac tggattaagc aaaacccaaa gtacaagtac cttgtgaaga
     1681 acaagaaagg ctctccaggc ctcacgcggc gaatgtctaa atccccagtt gacagctctg
     1741 tcctggaagg ggtgaaggac ctgtacctgg aggctgccca ccaaaatggt gacatgatcg
     1801 acccagacct gcagacgggt cttggagttc tgttccacct gagtggagag ttcaaccgag
     1861 cgatcgatgc atttaatgct gccttaactg ttcggccaga ggattactcg ttgtggaacc
     1921 gacttggggc aaccttggca aatggagacc gcagtgagga agctgtagag gcctacacga
     1981 gagcactgga gatccagcca ggtttcatcc ggtccaggta caacctggga atcagctgca
     2041 tcaacctggg tgcctacaga gaagcggtca gcaactttct cactgccctc agcttgcaga
     2101 ggaagagcag gaaccagcag caggtccctc accctgccat ctccgggaac atctgggccg
     2161 ccctcaggat tgcgctgtcg ctgatggacc agccagagct cttccaggca gccaacctcg
     2221 gcgacctgga tgtcctcctg agagctttca acttggatcc ttgaaggaaa agaaaagaaa
     2281 taaaggataa taacgcctca tctgtgttat tatactaaaa aagagacaaa actattttat
     2341 tatgaacttc caaaaaagaa attttttaag gattaaagct gagagtcaaa aggccatggt
     2401 catataaccc aagggaattg attcctacag ccccagtctc tgttcagacc caaaagcaca
     2461 aagtgaaggc cgggttccag agaagaactg agatcctcag gacaaaccaa gataaggtaa
     2521 ctatgttctc ttccaaattg caagtgtact ttgagtgatg ctgcccctat cccagccggt
     2581 ggcacatcta agggacattc gtgggacata acacaacaat ctgtccacag tcagtgaact
     2641 caggctgtct ctaaaccgtc tccccctttt gaaagactga acatagtttg ggccatttgc
     2701 aaaaggacat ggcttccctc acgcccattc taactttgac tgtaagcctt tgttttcaga
     2761 gagagagaga gagctttgct ggcaaaagca gtttttgcac tagaagtttt tgctgttccc
     2821 aatgaaatcc tttctagggt tgtacataag cactttacta tgttatttat gccttgccgg
     2881 gcttctgact tgctgctcca gtttctaact cggggagggg gaagggagtg tggggttgaa
     2941 agatttgaga actcagagtt gttagctcag tcagtggacc aaataaaaaa gctataagaa
     3001 taggtttttt taagtgtagg gtttggaagg tgtgtttgca cagaatgtaa atatgttagc
     3061 ccagttctct ctgtgtgctt cctgggagtc catggatctc ggagggaagc aaagctcgga
     3121 tagcattctc cctgcagagg ggagcttggt gattctgcga tttctaatga gttgtgttga
     3181 actgttttcc tatcctgttt tcctggcttt ctaactgcct tgcacaacga tacttccttt
     3241 aaaccgttac tattccaaat ggtatttctt aagcgtgata aatacaatgt tcctaaagtt
     3301 gatgtgtttc ctgggttccg tactaacccc cactgtccat gtcctcacat tgtaagtgac
     3361 agaaggctca catctgtaac ccagcagcac agagcagcag aggtctcatc tggaagagac
     3421 aagtagcacc cagcgagagc caggccttct ccaatggccg agaaatctga cttaattgtt
     3481 ggccttctac atatttctcc agattacttg gtgggtgttt ggcacttggt ggtcatttca
     3541 aaagatgcat tcattaaaaa attagaaaaa tgttggcaga acactaacca aagtaacatt
     3601 ttgctcagct cgaattctag taaattaccc tattcttctt ccctctgtag cctgcctgcg
     3661 tccagaatca tcctgaaagt agtttcatgc ccattgctta ttaatatctc actttagctt
     3721 ccaaactttg ggtcaaagcc gatattgact gcagaatcat ctactaatga tagaaaatgt
     3781 gccagaggct gacctctctg ccactgtgcc attccctgga ttgggggatg cctgaagttc
     3841 ataggtcact acaagtcctt gaagctcatc tatcacacag gcttcctggc tcacggggac
     3901 aaggtgataa gaccactgta gtggcctcaa ccttcactct tcactgctaa tattccttat
     3961 ggcagagcag tagccccagg ttggcagcag gcattgggag gcatcggggc atccacttct
     4021 gggcacggaa acaagtcaca cactttctaa tagcatcaag aacagcttga gtgtatttcg
     4081 aagagcagaa ttttctagac tgttagaaac agattgggaa gacgtcttcg ggccatactg
     4141 cagacagtgg ccttgcctgc tgggacattg ctgatagtgc agtgaccttt cctgccttct
     4201 ttctcactcc aagggtcaca tactgaccat gtgactcccc cagagacccc tacaagcctc
     4261 tgaaccaagg gttcaaagaa cgtttcacaa ctcttctggt cctaatgctc tccggggctc
     4321 tccgtggcac ctggtcttgc caggatgatg acccacctcg gccttgtcgc ttcacatgga
     4381 gatcagtcac ggcccgactg gtacaaatat gagaaacccg atttcatttt atgatgctga
     4441 gcagtgtcaa atatggtggc ccaaggtccg tgttgccatc agaagaccaa gtcacttcaa
     4501 actccaggga gatgttttag gggacaatta agagtgttcc gctccaccat gagggagcag
     4561 tgtgtaggac cttatccact ccttcggttc tatccatcac agtccagatg tttataggct
     4621 accacttgac aggaagttca aaggcatcac ctaagaggga aagagttgct atcctaatga
     4681 gaataaaaaa taataactaa aaagtaaaac tttgtataaa tagagcccta gcagacacta
     4741 agcagtgagc ttcagtgtct tccttctctg cttccctccc cactccccat gctttttaaa
     4801 tgcagaactg ttagggcttc tcatcctctt ggcatgaaat tgccttgtct ctttccattt
     4861 gaaacagtga ggcactcttc tgaggccctc aggagaagcc tgaggatgac attcattctt
     4921 ggagatggcc tcagtggctt ccctgaactg attcctccag taaattggat gaaaatccag
     4981 agaccattct tatcgaacat ctctatgcac agaagaggca ggatagaggg agactggggg
     5041 tgtagctcag tgggtactgt gtgtccacca ctacataaac tgaccatggt ggtgtacatt
     5101 gtaatcccag cacttggaaa gttcaagcag gaagatccgg gagcttaagg tcatcctggg
     5161 ctgtaggagg cgctgtagag acaaggagtg aagaaccttc taaaggagtc agtctctgtg
     5221 attctgtgtt ctctgcactt cattctgaca cccctcccaa ggacgcacca gaaactttaa
     5281 taactaacaa tgaagtgcac tttctcagcc agtctgattt ctgccagaat tgggtaggaa
     5341 tgtccacgat atacgatata ctcactctca gattaacata ccattttcat tgagaggttc
     5401 tgttatgttg acttgtagat cggtgtccat agtcttttac agggaagtca ttctgtgata
     5461 ttcccaccgt atgggctaga cttgaccata attgtggccc atctcacagt ttccaaacct
     5521 cttaacatag agtttacctg tgccctcctg gagaaagcag agaaaagagg atgcccactc
     5581 ctcacttgtc tctgaattgc ttaggttgaa acgcacatac attagtgttg agaggagggt
     5641 agggtctttt ttccccaccc tgacgtgctt ctaaaagcat tctagaaacc aggtgtctgg
     5701 aagatttaat ttgtgtgcca cctttctgag acctctgatt ttggaaaggg attcaggggc
     5761 aacacccatg acttcaacct tgtaacatag cttcacactg gctccccatg cagagtccct
     5821 accctacgtc tcacattgtt tataggactg gctgaattaa gcagttttga actaagaggt
     5881 agatccatat atttttaaag agtgttcata gatcaacaat ggcataaagt ttgtaaaaaa
     5941 aaaaaaaaaa agcagatcaa ctatggcata aagttcgtaa aaaaaaaagc aattttttta
     6001 aaaaagcaaa acatataacc tcaggtataa aactactgca tgcatcagta cttaaaacct
     6061 gcctatgact attaaccaaa gcatgcgaga ttacgtcact gaatgtgaat tggcccactc
     6121 acgccaagca tgactggtgt cataggctag cttgtgtttc catttttctc aacaagcaag
     6181 cacctcctaa ccttgttcca gttttttctc tttgcttttt ccatctcgtc tttcttgtag
     6241 atcattggcc tgctatcata ttggcattga ccatggctct caggttggtt catacattat
     6301 ggctgtcagt ctcaggccca ttctctgagg gactcaggta cagacgtgga tccttgaaaa
     6361 cacacttgag gctcaataga gctaaaagta tgaaaaggcc acacaactgt cactgcacaa
     6421 gaagttttga gctttaaatt cttaataatg gatttcaggt tttcttttca gaaagtttgg
     6481 gaagaaatag aaagcatttg ggattctaaa ctatctggga aggttaaagt gatgacacac
     6541 acatggagat tgccctaagc aaagcagaca tgggccctta gtttcccatt gagtggtttg
     6601 tcacagagta gcatgtaaag tgttgttcaa cttgcacggt caaagtccct ctcccttcat
     6661 aaactccatt tgatcaacct gatcacaaat gctcaaattt gggggtagaa gatggtttga
     6721 ttgtcagtgg aaatgtcatt tctgtgactt tttctgtaac tccaatttta tgaaaaggcc
     6781 aggaaaggag tgatttaagc tcagttcaat gaagctggtg ctggggtcag agttccaggg
     6841 gtacagtgct tacctaccat gtgcaaagct ctgggtttga tcccacacag cattcaactg
     6901 ggcctggtga cacccatctg taatcccagc acgtgggagg tagaggcagg aaaatcagga
     6961 agtcaaagac atccttggcc atacagaatt caaggccagc ctgtgttatg taagacactg
     7021 cctcgataaa tgaataaata aggccttcag gtcatatgtg aagaatgccc catgccctcc
     7081 agagacaacc ccagagtcta aactaccttt catacgcaca cagagaggcc acgtatgaag
     7141 atgtaaacgg ttagagcaaa gcaatagtaa atcaaagtgt gaggcgtaat agtctctcac
     7201 acggcgtgag gatgctctga ttgctgcaga aataccacat gactgagagc agtaaggggg
     7261 gagaacaagg aagccgcccg tggccgcgct gtgcaatgcg tatctttggg atcggctttg
     7321 gtttgccttt gaatggaggg gagagggaaa tggcatgcac acgatagatg acttgaattg
     7381 tgtcattcaa gcagtgtgtt cgtgttactg tcagtgaaag gacacaagcc taactcatca
     7441 aattccttct tttcccccat atctcatctg acccaaaccc ttctcaattt tcatcaccca
     7501 aatttgttta catcagttga gaaaaacaac tgatcaagtc agtaaacttt ttacagccta
     7561 cgagaagcat ttactactta gaaacatttc tatctatttt ctatatctgt gaatactgcg
     7621 tatctaatct aacctaagaa ttgtgtttta agaaaagcgc aatgatgcta ccttatgatg
     7681 ctcaatgttt aaaatcttgg gatttcattt tcaagctgaa ataggtaaca gaaaaactta
     7741 accttaaatg tttacattga atttctaaat taataccaat tccaaattaa gtgatctatg
     7801 ctcaattaaa ggccatgggg aatttgaaac ttattggcat tctatgagat actatttctg
     7861 tggcatagtg aataatacac agactattct gccatgtagt aagccttcag tgtagtcttt
     7921 ctctgcattg ccttttggaa taaaactatg tatcctaacc atgtatccta ctgaaatgtt
     7981 aattcacaga tgacctataa tttgccaagt actgagctta aggtagtgcg tttatacttt
     8041 attgaaggct cgccttcctt tttcacatta ttaacctgta tttacatttg tgtagtttta
     8101 aactatgttt ccaaataaac tttgtctgtg gcttcatggt cttttcagaa ctctctcaag
     8161 aagggatttg gggtcagacc acttaaagtc aaacagaaac caattactac tgctttaaga
     8221 agcctttctg tcgaatatca caacatataa ttttgcatga attatctgga aagcaggaat
     8281 gcatttgtag acaaagaaaa caccttgaaa accattttcc tttgttaatt ttttaagact
     8341 aaaatattaa cctgaaatcg aaatttgtga tctttttaga ataaaagcat taaaatatgt
     8401 gtca
//
