LOCUS       NM_019358               1852 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus podoplanin (Pdpn), mRNA.
ACCESSION   NM_019358
VERSION     NM_019358.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Furusawa,Y., Yamada,S., Itai,S., Nakamura,T., Fukui,M., Harada,H.,
            Kaneko,M.K. and Kato,Y.
  TITLE     Elucidation of Critical Epitope of Anti-Rat Podoplanin Monoclonal
            Antibody PMab-2
  JOURNAL   Monoclon Antib Immunodiagn Immunother 37 (4), 188-193 (2018)
   PUBMED   30088964
  REMARK    GeneRIF: the binding epitope of PMab-2 remains to be determined.
            Herein, we investigated the epitope of PMab-2 using enzyme-linked
            immunosorbent assay, immunohistochemical analysis, and flow
            cytometry. The results revealed that the critical epitope of PMab-2
            is Leu46 and Glu47 of rPDPN.
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Zakhary,I., Alotibi,F., Lewis,J., ElSalanty,M., Wenger,K.,
            Sharawy,M. and Messer,R.L.
  TITLE     Inherent physical characteristics and gene expression differences
            between alveolar and basal bones
  JOURNAL   Oral Surg Oral Med Oral Pathol Oral Radiol 122 (1), 35-42 (2016)
   PUBMED   27039006
  REMARK    GeneRIF: Four genes are expressed significantly higher in basal
            bone than in alveolar bone: SOST, E-11, DMP-1, and MEPE. Three of
            the proteins (SOST, MEPE, and DMP-1) are associated with mature
            osteocytes, indicating that basal bone has more mature osteocyte
            phenotypes because the immature osteocyte converts to a mature
            osteocyte as a result of bone mineralization.
REFERENCE   3  (bases 1 to 1852)
  AUTHORS   Martin-Villar,E., Borda-d'Agua,B., Carrasco-Ramirez,P., Renart,J.,
            Parsons,M., Quintanilla,M. and Jones,G.E.
  TITLE     Podoplanin mediates ECM degradation by squamous carcinoma cells
            through control of invadopodia stability
  JOURNAL   Oncogene 34 (34), 4531-4544 (2015)
   PUBMED   25486435
REFERENCE   4  (bases 1 to 1852)
  AUTHORS   Astarita,J.L., Cremasco,V., Fu,J., Darnell,M.C., Peck,J.R.,
            Nieves-Bonilla,J.M., Song,K., Kondo,Y., Woodruff,M.C., Gogineni,A.,
            Onder,L., Ludewig,B., Weimer,R.M., Carroll,M.C., Mooney,D.J.,
            Xia,L. and Turley,S.J.
  TITLE     The CLEC-2-podoplanin axis controls the contractility of
            fibroblastic reticular cells and lymph node microarchitecture
  JOURNAL   Nat Immunol 16 (1), 75-84 (2015)
   PUBMED   25347465
REFERENCE   5  (bases 1 to 1852)
  AUTHORS   Hirahara,I., Sato,H., Imai,T., Onishi,A., Morishita,Y., Muto,S.,
            Kusano,E. and Nagata,D.
  TITLE     Methylglyoxal Induced Basophilic Spindle Cells with Podoplanin at
            the Surface of Peritoneum in Rat Peritoneal Dialysis Model
  JOURNAL   Biomed Res Int 2015, 289751 (2015)
   PUBMED   26064894
  REMARK    GeneRIF: In rats treated with MGO, peritoneal fibrous thickening
            with the appearance of basophilic spindle cells with podoplanin,
            cytokeratin, and alpha-smooth muscle actin at the surface of the
            peritoneum was observed
REFERENCE   6  (bases 1 to 1852)
  AUTHORS   Breiteneder-Geleff,S., Matsui,K., Soleiman,A., Meraner,P.,
            Poczewski,H., Kalt,R., Schaffner,G. and Kerjaschki,D.
  TITLE     Podoplanin, novel 43-kd membrane protein of glomerular epithelial
            cells, is down-regulated in puromycin nephrosis
  JOURNAL   Am J Pathol 151 (4), 1141-1152 (1997)
   PUBMED   9327748
REFERENCE   7  (bases 1 to 1852)
  AUTHORS   Williams,M.C., Cao,Y., Hinds,A., Rishi,A.K. and Wetterwald,A.
  TITLE     T1 alpha protein is developmentally regulated and expressed by
            alveolar type I cells, choroid plexus, and ciliary epithelia of
            adult rats
  JOURNAL   Am J Respir Cell Mol Biol 14 (6), 577-585 (1996)
   PUBMED   8652186
REFERENCE   8  (bases 1 to 1852)
  AUTHORS   Wetterwald,A., Hoffstetter,W., Cecchini,M.G., Lanske,B., Wagner,C.,
            Fleisch,H. and Atkinson,M.
  TITLE     Characterization and cloning of the E11 antigen, a marker expressed
            by rat osteoblasts and osteocytes
  JOURNAL   Bone 18 (2), 125-132 (1996)
   PUBMED   8833206
REFERENCE   9  (bases 1 to 1852)
  AUTHORS   McElroy,M.C., Pittet,J.F., Hashimoto,S., Allen,L.,
            Wiener-Kronish,J.P. and Dobbs,L.G.
  TITLE     A type I cell-specific protein is a biochemical marker of
            epithelial injury in a rat model of pneumonia
  JOURNAL   Am J Physiol 268 (2 Pt 1), L181-L186 (1995)
   PUBMED   7864138
REFERENCE   10 (bases 1 to 1852)
  AUTHORS   Rishi,A.K., Joyce-Brady,M., Fisher,J., Dobbs,L.G., Floros,J.,
            VanderSpek,J., Brody,J.S. and Williams,M.C.
  TITLE     Cloning, characterization, and development expression of a rat lung
            alveolar type I cell gene in embryonic endodermal and neural
            derivatives
  JOURNAL   Dev Biol 167 (1), 294-306 (1995)
   PUBMED   7851650
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            [WARNING] On Apr 26, 2024 this sequence was replaced by
            NM_019358.3.
            
            On Nov 26, 2020 this sequence version replaced NM_019358.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U07797.1, BC072492.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840115, SAMN16676784
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-304               JAXUCZ010000005.1  160918656-160918959 c
            305-441             JAXUCZ010000005.1  160894379-160894515 c
            442-580             JAXUCZ010000005.1  160892035-160892173 c
            581-619             JAXUCZ010000005.1  160888548-160888586 c
            620-731             JAXUCZ010000005.1  160887046-160887157 c
            732-1852            JAXUCZ010000005.1  160884995-160886115 c
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..1852
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /note="podoplanin"
                     /db_xref="GeneID:54320"
                     /db_xref="RGD:61819"
     exon            1..304
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..738
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /note="glycoprotein 38; epithelial cell surface
                     transmembrane protein antigen; pulmonary type I alveolar
                     epithelial cell transmembrane differentiation marker; T1A;
                     RTI140; E11 antigen epitope; type I cell 40 kDa protein"
                     /codon_start=1
                     /product="podoplanin precursor"
                     /protein_id="NP_062231.2"
                     /db_xref="GeneID:54320"
                     /db_xref="RGD:61819"
                     /translation="MWTAPVLLWVLGSVWFWDSAQGGAIGALEDDLVTPGPGDDMVNP
                     GLEDRIETTDTTGELDKSTAKAPLVPTQPPIEELPTSGTSDHDHKEHESTTTVKAVTS
                     HSTDKKTTHSNRDNAGGETQTTDKKDGLAVVTLVGIIIGVLLAIGFIGGIIIVVMRKI
                     SGRFSP"
     exon            305..441
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            442..580
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            581..619
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            620..731
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            732..1852
                     /gene="Pdpn"
                     /gene_synonym="E11; Gp38; OTS-8; RTI40; T1-alpha"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagacataa attgccgact gtgctgagaa gttgccagtt gccaagtttg ctgtgcttgc
       61 tccctccgct cgcattgaaa gcccgggcac tctctgtcgc tgggactctt gctcaccgct
      121 tgccagcctc ctccagagct cttctcggct gggcctgtgg cttcgaagtt tttttgtttt
      181 ttcatctttt cacaacccac aaaaaccact agctgctgag gctccaacga gatcaagatg
      241 tggaccgcgc cagtgttgct ctgggttttg gggagcgttt ggttctggga ctcagcgcag
      301 ggaggggcca tcggtgcgct agaagatgat cttgtgaccc cgggtccagg agatgacatg
      361 gtgaacccag gtctagaaga cagaatagaa accacggaca ctacaggaga actggataag
      421 tcaactgcca aggcgcctct ggtaccaaca cagcctccca ttgaggaact gccgacctca
      481 ggaacctccg accacgatca caaagaacat gagagtacaa ccactgtcaa agcggtgacc
      541 agccactcca cggacaagaa aacaactcac tccaatagag ataacgcagg cggtgaaacg
      601 cagaccacag ataagaaaga tggcttggct gtagtgaccc tggtcggaat cataattggc
      661 gtcttgttag ccattggctt cattggaggg atcatcattg tggttatgag gaagatttct
      721 ggaaggttct cgccctaaag agccaaacag aactggttgt tctcccaaca catctgaaaa
      781 taagagagct tccaccttgc cctgttcccc atcctcttcc tgtggaggaa gatgacccat
      841 ggaatgccca cccacccctc tcacagccat ggtgacccct ccacttgcca gcagtaccaa
      901 aggaaagata cgggacaagc cactggcccc tcgaaccatc tgcctttgga aatcaaattt
      961 tttaatataa atgttatgat tgaggaccac atgcatagaa aaatggtgca aaaaccgaga
     1021 cagtatcatc agctttatca actgtaacca tgggttggtt cttccgggcc agtcccagtc
     1081 tgttaagagg caaaagaatt tggaaatgtt acctcacaga ggcacgggtc tttttgcagt
     1141 tgccaacctg caaatgcagt tgatgcatac atattccagc ctagtggggt ctgaatcagt
     1201 gcattgaaag tataaaccca aaatcaggcg tgataggcag cccctcaggg agcttcatta
     1261 taccttttca gaagcatcca ctgccttcag ttcccggaag ataccttgct ttttacaagc
     1321 ctccaaaccc tctctaagcc tcagatgact caatgaccca gaggtctgtc tactccactc
     1381 cccctggtga tggaaagccc aagttgagga accatgtcct cacctcccta ggcctcaagc
     1441 cttcagatca accgcttctt tctggacgat ggagacctca tcccagatgc tcagaaagtt
     1501 tgttggatgt cacaaaatgc cagagcctca gccgctgtcg aaccaagaac gaaactcagt
     1561 ccttaagtgg aagtgactcc tgtgcctgct ttagcgtagg aaatgagacc aaccagtttc
     1621 tagcacctgc cttctgcttc ccaggaaaga cggagaaaaa aataatcttg tatgttctta
     1681 actgcttcta ggtggtggat ggaaaggttt gatgttaatt tccggtcttc gtgacatacc
     1741 acatcttccc atgaggactc ggtgttgctg gcacaagagc tgtagtgtct tggttacctg
     1801 tgttggtact caatacagtg tagacatctt ttcaaataaa atatgattgt gt
//
