LOCUS       NM_019372               2515 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus pyruvate dehydrogenase phosphatase catalytic
            subunit 1 (Pdp1), transcript variant 2, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_019372
VERSION     NM_019372.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Feng,X., Lu,J., Wu,Y. and Xu,H.
  TITLE     MiR-18a-3p improves cartilage matrix remodeling and inhibits
            inflammation in osteoarthritis by suppressing PDP1
  JOURNAL   J Physiol Sci 72 (1), 3 (2022)
   PUBMED   35148687
  REMARK    GeneRIF: MiR-18a-3p improves cartilage matrix remodeling and
            inhibits inflammation in osteoarthritis by suppressing PDP1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Xing,G., Ren,M., O'Neill,J.T., Sharma,P., Verma,A. and Watson,W.D.
  TITLE     Pyruvate dehydrogenase phosphatase1 mRNA expression is divergently
            and dynamically regulated between rat cerebral cortex, hippocampus
            and thalamus after traumatic brain injury: a potential biomarker of
            TBI-induced hyper- and hypo-glycaemia and neuronal vulnerability
  JOURNAL   Neurosci Lett 525 (2), 140-145 (2012)
   PUBMED   22884618
  REMARK    GeneRIF: divergent alterations of PDP1 mRNA expression were found
            in different brain regions following traumatic brain injury.
REFERENCE   3  (bases 1 to 2515)
  AUTHORS   Voss,M., Paterson,J., Kelsall,I.R., Martin-Granados,C.,
            Hastie,C.J., Peggie,M.W. and Cohen,P.T.
  TITLE     Ppm1E is an in cellulo AMP-activated protein kinase phosphatase
  JOURNAL   Cell Signal 23 (1), 114-124 (2011)
   PUBMED   20801214
  REMARK    Erratum:[Cell Signal. 2011 May;23(5):947]
REFERENCE   4  (bases 1 to 2515)
  AUTHORS   Leblanc,P.J., Mulligan,M., Antolic,A., Macpherson,L., Inglis,J.G.,
            Martin,D., Roy,B.D. and Peters,S.J.
  TITLE     Skeletal muscle type comparison of pyruvate dehydrogenase
            phosphatase activity and isoform expression: effects of obesity and
            endurance training
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 295 (4), R1224-R1230 (2008)
   PUBMED   18716035
  REMARK    GeneRIF: Results suggest that decreased PDP activity in oxidative
            skeletal muscles may play a role in the impairment of carbohydrate
            metabolism in obese rats, which is reversible with endurance
            training.
REFERENCE   5  (bases 1 to 2515)
  AUTHORS   Vassylyev,D.G. and Symersky,J.
  TITLE     Crystal structure of pyruvate dehydrogenase phosphatase 1 and its
            functional implications
  JOURNAL   J Mol Biol 370 (3), 417-426 (2007)
   PUBMED   17532339
  REMARK    GeneRIF: Results report the crystal structure of the rat pyruvate
            dehydrogenase phosphatase 1 catalytic subunit at 1.8 A resolution.
REFERENCE   6  (bases 1 to 2515)
  AUTHORS   Piccinini,M., Mostert,M., Alberto,G., Ramondetti,C., Novi,R.F.,
            Dalmasso,P. and Rinaudo,M.T.
  TITLE     Down-regulation of pyruvate dehydrogenase phosphatase in obese
            subjects is a defect that signals insulin resistance
  JOURNAL   Obes Res 13 (4), 678-686 (2005)
   PUBMED   15897476
REFERENCE   7  (bases 1 to 2515)
  AUTHORS   Wang,M.Y. and Unger,R.H.
  TITLE     Role of PP2C in cardiac lipid accumulation in obese rodents and its
            prevention by troglitazone
  JOURNAL   Am J Physiol Endocrinol Metab 288 (1), E216-E221 (2005)
   PUBMED   15367397
  REMARK    GeneRIF: role of PP2C and AMPK in the derangements of cardiac lipid
            metabolism in obesity
REFERENCE   8  (bases 1 to 2515)
  AUTHORS   Karpova,T., Danchuk,S., Huang,B. and Popov,K.M.
  TITLE     Probing a putative active site of the catalytic subunit of pyruvate
            dehydrogenase phosphatase 1 (PDP1c) by site-directed mutagenesis
  JOURNAL   Biochim Biophys Acta 1700 (1), 43-51 (2004)
   PUBMED   15210124
  REMARK    GeneRIF: Asp445 might be involved in the control of interactions
            between PDP1c and the protein substrate pyruvate dehydrogenase
REFERENCE   9  (bases 1 to 2515)
  AUTHORS   Huang,B., Wu,P., Popov,K.M. and Harris,R.A.
  TITLE     Starvation and diabetes reduce the amount of pyruvate dehydrogenase
            phosphatase in rat heart and kidney
  JOURNAL   Diabetes 52 (6), 1371-1376 (2003)
   PUBMED   12765946
REFERENCE   10 (bases 1 to 2515)
  AUTHORS   Huang,B., Gudi,R., Wu,P., Harris,R.A., Hamilton,J. and Popov,K.M.
  TITLE     Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino
            acid sequences, expression, and regulation
  JOURNAL   J Biol Chem 273 (28), 17680-17688 (1998)
   PUBMED   9651365
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FN800686.1 and JAXUCZ010000005.1.
            
            [WARNING] On Apr 26, 2024 this sequence was replaced by
            NM_019372.5.
            
            On Sep 4, 2012 this sequence version replaced NM_019372.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream AUG, compared to variant 1. The encoded isoform (2) has
            a shorter N-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF062740.1, SRR22582416.377840.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840111 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-651               FN800686.1         1-651
            652-2515            JAXUCZ010000005.1  30245699-30247562   c
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q13"
     gene            1..2515
                     /gene="Pdp1"
                     /gene_synonym="Ppm2c"
                     /note="pyruvate dehydrogenase phosphatase catalytic
                     subunit 1"
                     /db_xref="GeneID:54705"
                     /db_xref="RGD:620393"
     exon            1..100
                     /gene="Pdp1"
                     /gene_synonym="Ppm2c"
                     /inference="alignment:Splign:2.1.0"
     exon            101..2515
                     /gene="Pdp1"
                     /gene_synonym="Ppm2c"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..1761
                     /gene="Pdp1"
                     /gene_synonym="Ppm2c"
                     /EC_number="3.1.3.43"
                     /note="isoform 2 is encoded by transcript variant 2;
                     [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase
                     1, mitochondrial; PDP 1; PDPC 1; pyruvate dehydrogenase
                     phosphatase isoenzyme 1; protein phosphatase 2C, magnesium
                     dependent, catalytic subunit; pyruvate dehyrogenase
                     phosphatase catalytic subunit 1"
                     /codon_start=1
                     /product="[Pyruvate dehydrogenase
                     [acetyl-transferring]]-phosphatase 1, mitochondrial
                     isoform 2"
                     /protein_id="NP_062245.2"
                     /db_xref="GeneID:54705"
                     /db_xref="RGD:620393"
                     /translation="MPAPTQLFFPLVRNCELSRIYGTACYCHHKHLCCSPPYIPQNRL
                     RYTPHPAYATFCRPRENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFD
                     GKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLF
                     YYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQE
                     LIDLNTGESADIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACV
                     AHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKNEA
                     KSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTP
                     PYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGG
                     YKVTLGQMHGLLTERRAKMSSVFEDQNAATHLIRHAVGNNEFGAVDHERLSKMLSLPE
                     ELARMYRDDITIIVVQFNSHVVGAYQNQEQ"
ORIGIN      
        1 ccgcgcgcca gggtgctgcc gtggtgctga gaaaaggacg cgcacggagg ggtgcggccg
       61 ccacccgggg cagacagcag cgggtgctct actgtgcccg gaatcccggt cagaacatcc
      121 agtctaccac tgttctctga tgccatgcca gcaccaactc aactgttttt tcctctcgtc
      181 cgtaactgtg aactgagcag aatctatggc actgcatgtt actgccacca caaacatctc
      241 tgctgttcac caccgtacat tcctcagaat cgtctgagat acacacccca tccagcatat
      301 gctacctttt gtaggccaag ggagaactgg tggcagtata ctcaaggaag gagatatgct
      361 tctacgcctc agaaatttta cctcacacct ccacaagtca acagcatcct taaagctaat
      421 gaatacagct tcaaagtacc agaatttgat ggcaaaaatg tcagttccat tcttggattt
      481 gacagcaatc agctgcctgc aaatgcaccc atagaagacc ggagaagtgc agcaacctgc
      541 ttgcagacca gagggatgct tttgggcgtt tttgatggtc atgcaggctg tgcttgctcc
      601 caggcagtca gtgaaagact cttctattat attgctgttt ccttgttacc ccatgagact
      661 ttgctagaga ttgaaaatgc agtggagagt ggtcgggcac tgctacctat ccttcagtgg
      721 cacaagcacc ccaatgatta cttcagtaag gaggcatcca aattgtattt caacagcttg
      781 aggacttact ggcaagagct tatagacctc aatactggag aatcagctga tattgatgtt
      841 aaggaggctt taattaatgc tttcaagaga cttgataatg acatttcatt ggaggctcaa
      901 gtcggtgatc ctaattcttt tctcaattac ctggtgcttc gggtagcatt ttctggggct
      961 actgcttgtg tggcccatgt agatggtgtt gacctccatg tggctaacac tggcgatagt
     1021 agagccatgc taggtgtgca agaagaagat ggctcctggt cagcagtcac gctctctaat
     1081 gaccacaatg ctcagaatga aagagaacta gaacgtctaa aactggaaca cccaaaaaat
     1141 gaggccaaga gcgtggtaaa gcaggatcgg ctgcttggct tgctgatgcc ctttagggct
     1201 tttggggatg taaagttcaa atggagcatt gaccttcaaa agagagtgat agagtctggc
     1261 ccagaccagt tgaatgacaa tgaatacacc aagtttatcc ctcctaacta tcatacacct
     1321 ccttatctca ctgctgagcc agaggtaact tatcaccgat taaggccaca ggataaattc
     1381 ctagtgttag caactgatgg attgtgggag actatgcata gacaggatgt ggttaggatt
     1441 gtgggtgagt acttaactgg tatgcatcac caacagccaa tagctgttgg tgggtacaag
     1501 gtgactctgg gacagatgca tggcctttta acagaaagga gagcaaagat gtcatcagtc
     1561 tttgaggatc agaatgcagc aacccatctc attcgccatg ctgtaggcaa taatgaattt
     1621 ggagctgttg atcatgaacg actctctaaa atgcttagcc ttcctgaaga gcttgctcgg
     1681 atgtatagag atgacattac aatcattgta gttcagttca attctcatgt tgtaggagca
     1741 tatcaaaacc aggaacagta agtgatacta tcctggcagt tcttcagttt gaagggcaga
     1801 tcaatgttca aaagaaacta atacagtaaa tatcccagtg ggtcattcta aacacatcat
     1861 gtttagtact ctagctagtc ttcatatcta ctgcatcaga tgatagcatc atgagtgtct
     1921 gttctgtcct gttggacctc agggtacctg cacttgaggc agcttgtttc cttacccagg
     1981 tgtcttttaa caatgactca ccaactaaga atatggatag gtagatacga tcttgaatag
     2041 gtcaaaagca aggaacttct gggagtatta gtaaaggtag aaaacatcac catgcccacc
     2101 tgcagactcc ttccatcata agactcctaa tgtacatgag aatagttatt tactgcattt
     2161 ttcagatgaa cagttcaggt attcacatac atttatgtta gcctaaagtg caggtccagt
     2221 attttcagcc atttatgacc atgaacaaag aaatctggat ttgtaagttt ttgtgattgt
     2281 gtgctaaagt atgcttcctg aacggaaaga attctaaacc gttcttcctt cctcacccat
     2341 gatttcctca gtgtaaccga aatattttgt tttgctacac ctaaaagaca ataaagattc
     2401 ttagtttaaa tctatctttc tagtgaatgt taaataaagg gaaaatgtgt ttttctggtt
     2461 tgtattcatg aaaagcttta tttagtttga aataaagata tatgcagaat gaaaa
//
