LOCUS       NM_053551               1425 bp    mRNA    linear   ROD 13-OCT-2024
DEFINITION  Rattus norvegicus pyruvate dehydrogenase kinase 4 (Pdk4), mRNA.
ACCESSION   NM_053551
VERSION     NM_053551.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Chen,T., Xie,Q., Tan,B., Yi,Q., Xiang,H., Wang,R., Zhou,Q., He,B.,
            Tian,J., Zhu,J. and Xu,H.
  TITLE     Inhibition of Pyruvate Dehydrogenase Kinase 4 Protects
            Cardiomyocytes from lipopolysaccharide-Induced Mitochondrial Damage
            by Reducing Lactate Accumulation
  JOURNAL   Inflammation 47 (4), 1356-1370 (2024)
   PUBMED   38401019
  REMARK    GeneRIF: Inhibition of Pyruvate Dehydrogenase Kinase 4 Protects
            Cardiomyocytes from lipopolysaccharide-Induced Mitochondrial Damage
            by Reducing Lactate Accumulation.
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Tan,W., Bao,H., Liu,Z., Liu,Y., Hong,L. and Shao,L.
  TITLE     Protein PDK4 Interacts with HMGCS2 to Facilitate High
            Glucoseinduced Myocardial Injuries
  JOURNAL   Curr Mol Med 23 (10), 1104-1115 (2023)
   PUBMED   36281857
  REMARK    GeneRIF: Protein PDK4 Interacts with HMGCS2 to Facilitate High
            Glucoseinduced Myocardial Injuries.
REFERENCE   3  (bases 1 to 1425)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 1425)
  AUTHORS   Yin,Q., Wang,P. and Wu,X.
  TITLE     MicroRNA -148 alleviates cardiac dysfunction, immune disorders and
            myocardial apoptosis in myocardial ischemia-reperfusion (MI/R)
            injury by targeting pyruvate dehydrogenase kinase (PDK4)
  JOURNAL   Bioengineered 12 (1), 5552-5565 (2021)
   PUBMED   34517782
  REMARK    GeneRIF: MicroRNA -148 alleviates cardiac dysfunction, immune
            disorders and myocardial apoptosis in myocardial
            ischemia-reperfusion (MI/R) injury by targeting pyruvate
            dehydrogenase kinase (PDK4).
REFERENCE   5  (bases 1 to 1425)
  AUTHORS   Ma,W.Q., Sun,X.J., Zhu,Y. and Liu,N.F.
  TITLE     PDK4 promotes vascular calcification by interfering with autophagic
            activity and metabolic reprogramming
  JOURNAL   Cell Death Dis 11 (11), 991 (2020)
   PUBMED   33203874
  REMARK    GeneRIF: PDK4 promotes vascular calcification by interfering with
            autophagic activity and metabolic reprogramming.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1425)
  AUTHORS   Patel,M.S. and Korotchkina,L.G.
  TITLE     Regulation of mammalian pyruvate dehydrogenase complex by
            phosphorylation: complexity of multiple phosphorylation sites and
            kinases
  JOURNAL   Exp Mol Med 33 (4), 191-197 (2001)
   PUBMED   11795479
  REMARK    Review article
REFERENCE   7  (bases 1 to 1425)
  AUTHORS   Korotchkina,L.G. and Patel,M.S.
  TITLE     Site specificity of four pyruvate dehydrogenase kinase isoenzymes
            toward the three phosphorylation sites of human pyruvate
            dehydrogenase
  JOURNAL   J Biol Chem 276 (40), 37223-37229 (2001)
   PUBMED   11486000
REFERENCE   8  (bases 1 to 1425)
  AUTHORS   Kolobova,E., Tuganova,A., Boulatnikov,I. and Popov,K.M.
  TITLE     Regulation of pyruvate dehydrogenase activity through
            phosphorylation at multiple sites
  JOURNAL   Biochem J 358 (Pt 1), 69-77 (2001)
   PUBMED   11485553
REFERENCE   9  (bases 1 to 1425)
  AUTHORS   Sugden,M.C., Kraus,A., Harris,R.A. and Holness,M.J.
  TITLE     Fibre-type specific modification of the activity and regulation of
            skeletal muscle pyruvate dehydrogenase kinase (PDK) by prolonged
            starvation and refeeding is associated with targeted regulation of
            PDK isoenzyme 4 expression
  JOURNAL   Biochem J 346 Pt 3 (Pt 3), 651-657 (2000)
   PUBMED   10698691
REFERENCE   10 (bases 1 to 1425)
  AUTHORS   Bowker-Kinley,M.M., Davis,W.I., Wu,P., Harris,R.A. and Popov,K.M.
  TITLE     Evidence for existence of tissue-specific regulation of the
            mammalian pyruvate dehydrogenase complex
  JOURNAL   Biochem J 329 (Pt 1) (Pt 1), 191-196 (1998)
   PUBMED   9405293
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Nov 26, 2020 this sequence version replaced NM_053551.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF034577.1, FQ216954.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760494,
                                           SAMN06621351 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-225               JAXUCZ010000004.1  34568105-34568329   c
            226-367             JAXUCZ010000004.1  34567037-34567178   c
            368-439             JAXUCZ010000004.1  34565679-34565750   c
            440-624             JAXUCZ010000004.1  34564776-34564960   c
            625-711             JAXUCZ010000004.1  34564533-34564619   c
            712-789             JAXUCZ010000004.1  34564011-34564088   c
            790-866             JAXUCZ010000004.1  34562105-34562181   c
            867-965             JAXUCZ010000004.1  34560349-34560447   c
            966-1076            JAXUCZ010000004.1  34560055-34560165   c
            1077-1190           JAXUCZ010000004.1  34559650-34559763   c
            1191-1425           JAXUCZ010000004.1  34558327-34558561   c
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q21"
     gene            1..1425
                     /gene="Pdk4"
                     /note="pyruvate dehydrogenase kinase 4"
                     /db_xref="GeneID:89813"
                     /db_xref="RGD:69061"
     exon            1..225
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1334
                     /gene="Pdk4"
                     /EC_number="2.7.11.2"
                     /note="[Pyruvate dehydrogenase [lipoamide]] kinase isozyme
                     4, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme
                     4; pyruvate dehydrogenate kinase 4; [Pyruvate
                     dehydrogenase (acetyl-transferring)] kinase isozyme 4,
                     mitochondrial"
                     /codon_start=1
                     /product="pyruvate dehydrogenase kinase, isozyme 4"
                     /protein_id="NP_446003.2"
                     /db_xref="GeneID:89813"
                     /db_xref="RGD:69061"
                     /translation="MKAARFVMRSASSLGNAGLVPREVELFSRYSPSPLSMKQLLDFG
                     SENACERTSFSFLRQELPVRLANILKEIDILPERLVNTPSVQLVKSWYIQSLMDLVEF
                     HEKSPEDQKALSDFVDTLVKVRNRHHNVVPTMAQGILEYKDNCTVDPVTNQNLQYFLD
                     RFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVEDAFECAKMLCDQY
                     YLTSPELKLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPV
                     EATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGL
                     PISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEA
                     DDWCIPSKEPKNLSKEKLAV"
     exon            226..367
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            368..439
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            440..624
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            625..711
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            712..789
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            790..866
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            867..965
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1076
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1190
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1425
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacagcgcac ttgctctcgc tcgaccgcgc tcccgccccg cagtcctcac caaccctacg
       61 gatcctagcc accgccagcc taggagggca tcaggatgaa ggcagcccgt ttcgtgatgc
      121 gcagcgccag ctcgctgggc aacgccggcc tggtccccag ggaggtcgag ctgttctccc
      181 gctacagccc gtccccgctg tccatgaagc agctgctgga cttcggttca gaaaatgcct
      241 gtgagagaac ttctttttct tttcttcggc aagaattgcc cgtcagactt gccaacatcc
      301 tgaaggagat tgacatcctc cctgaacgct tagtgaacac cccttccgtc cagctggtga
      361 agagctggta catccagagc ctgatggatt tagtggagtt ccacgaaaag agcccagaag
      421 accagaaagc tctatcagac tttgtagaca cgctggtcaa agttcgaaac agacatcata
      481 atgtggtccc tacgatggca caagggatcc tggagtacaa agacaactgc accgttgacc
      541 ccgttaccaa tcaaaatctt cagtattttt tggaccggtt ttatatgaac cgcatttcta
      601 ctcggatgct catgaaccag cacatcctca tattcagtga ctcaaagaca ggaaacccaa
      661 gccacattgg gagtatcgac cccaactgcg atgtggtagc agtagtcgaa gatgcctttg
      721 agtgtgcaaa gatgctctgc gatcagtatt atctaacgtc gccagaatta aagctcacac
      781 aagtcaatgg aaaatttcca ggccaaccaa tccacatcgt gtacgttcct tcacaccttc
      841 accacatgct ctttgaacta ttcaagaatg ccatgagggc cacggtcgag catcaagaaa
      901 accgcccttc cctgacacca gtcgaggcca ccgtcgtctt gggaaaagaa gatcttacaa
      961 tcaagatttc tgaccgagga ggcggtgttc ctctgaggat tactgaccgc ctctttagtt
     1021 acacatactc cactgctcca acgcctgtga tggacaattc acggaatgcc cctttggctg
     1081 gttttggtta cggcttgccg atttcccgtc tctacgccaa gtattttcaa ggagatctga
     1141 atctctattc catgtcaggc tatgggacag atgccatcat ctacttaaag gctttatcat
     1201 ctgagtctat agaaaaactc cccgtcttta acaagtcagc cttcaaacat tatcagatga
     1261 gttctgaggc tgatgactgg tgtatcccaa gtaaggaacc aaagaatctg tcgaaggaga
     1321 aactggcagt gtgacgggga tctcgcctgg cactttacgg gatcaaagtg ggtctacggc
     1381 agtgctgctt cgtgaatgtg tgtggactct tgtttccgca aaaca
//
