LOCUS       NM_001106581            2078 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus pyruvate dehydrogenase kinase 3 (Pdk3), mRNA.
ACCESSION   NM_001106581 XM_001056460 XM_039099568 XM_216091
VERSION     NM_001106581.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Tian,L., Wu,D., Dasgupta,A., Chen,K.H., Mewburn,J., Potus,F.,
            Lima,P.D.A., Hong,Z., Zhao,Y.Y., Hindmarch,C.C.T., Kutty,S.,
            Provencher,S., Bonnet,S., Sutendra,G. and Archer,S.L.
  TITLE     Epigenetic Metabolic Reprogramming of Right Ventricular Fibroblasts
            in Pulmonary Arterial Hypertension: A Pyruvate Dehydrogenase
            Kinase-Dependent Shift in Mitochondrial Metabolism Promotes Right
            Ventricular Fibrosis
  JOURNAL   Circ Res 126 (12), 1723-1745 (2020)
   PUBMED   32216531
  REMARK    GeneRIF: Epigenetic Metabolic Reprogramming of Right Ventricular
            Fibroblasts in Pulmonary Arterial Hypertension: A Pyruvate
            Dehydrogenase Kinase-Dependent Shift in Mitochondrial Metabolism
            Promotes Right Ventricular Fibrosis.
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Kluza,J., Corazao-Rozas,P., Touil,Y., Jendoubi,M., Maire,C.,
            Guerreschi,P., Jonneaux,A., Ballot,C., Balayssac,S., Valable,S.,
            Corroyer-Dulmont,A., Bernaudin,M., Malet-Martino,M., de
            Lassalle,E.M., Maboudou,P., Formstecher,P., Polakowska,R.,
            Mortier,L. and Marchetti,P.
  TITLE     Inactivation of the HIF-1alpha/PDK3 signaling axis drives melanoma
            toward mitochondrial oxidative metabolism and potentiates the
            therapeutic activity of pro-oxidants
  JOURNAL   Cancer Res 72 (19), 5035-5047 (2012)
   PUBMED   22865452
REFERENCE   3  (bases 1 to 2078)
  AUTHORS   Akhmedov,D., De Marchi,U., Wollheim,C.B. and Wiederkehr,A.
  TITLE     Pyruvate dehydrogenase E1alpha phosphorylation is induced by
            glucose but does not control metabolism-secretion coupling in
            INS-1E clonal beta-cells
  JOURNAL   Biochim Biophys Acta 1823 (10), 1815-1824 (2012)
   PUBMED   22809973
REFERENCE   4  (bases 1 to 2078)
  AUTHORS   Blouin,J.M., Penot,G., Collinet,M., Nacfer,M., Forest,C.,
            Laurent-Puig,P., Coumoul,X., Barouki,R., Benelli,C. and Bortoli,S.
  TITLE     Butyrate elicits a metabolic switch in human colon cancer cells by
            targeting the pyruvate dehydrogenase complex
  JOURNAL   Int J Cancer 128 (11), 2591-2601 (2011)
   PUBMED   20715114
REFERENCE   5  (bases 1 to 2078)
  AUTHORS   Lu,C.W., Lin,S.C., Chen,K.F., Lai,Y.Y. and Tsai,S.J.
  TITLE     Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible
            factor-1 promotes metabolic switch and drug resistance
  JOURNAL   J Biol Chem 283 (42), 28106-28114 (2008)
   PUBMED   18718909
REFERENCE   6  (bases 1 to 2078)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2078)
  AUTHORS   Patel,M.S. and Korotchkina,L.G.
  TITLE     Regulation of mammalian pyruvate dehydrogenase complex by
            phosphorylation: complexity of multiple phosphorylation sites and
            kinases
  JOURNAL   Exp Mol Med 33 (4), 191-197 (2001)
   PUBMED   11795479
  REMARK    Review article
REFERENCE   8  (bases 1 to 2078)
  AUTHORS   Korotchkina,L.G. and Patel,M.S.
  TITLE     Site specificity of four pyruvate dehydrogenase kinase isoenzymes
            toward the three phosphorylation sites of human pyruvate
            dehydrogenase
  JOURNAL   J Biol Chem 276 (40), 37223-37229 (2001)
   PUBMED   11486000
REFERENCE   9  (bases 1 to 2078)
  AUTHORS   Kolobova,E., Tuganova,A., Boulatnikov,I. and Popov,K.M.
  TITLE     Regulation of pyruvate dehydrogenase activity through
            phosphorylation at multiple sites
  JOURNAL   Biochem J 358 (Pt 1), 69-77 (2001)
   PUBMED   11485553
REFERENCE   10 (bases 1 to 2078)
  AUTHORS   Baker,J.C., Yan,X., Peng,T., Kasten,S. and Roche,T.E.
  TITLE     Marked differences between two isoforms of human pyruvate
            dehydrogenase kinase
  JOURNAL   J Biol Chem 275 (21), 15773-15781 (2000)
   PUBMED   10748134
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On or before Jun 3, 2021 this sequence version replaced
            XM_039099568.1, NM_001106581.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC169078.1, SRR26643289.44861.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-299               JAXUCZ010000021.1  62547073-62547371   c
            300-441             JAXUCZ010000021.1  62527495-62527636   c
            442-513             JAXUCZ010000021.1  62521539-62521610   c
            514-698             JAXUCZ010000021.1  62516605-62516789   c
            699-788             JAXUCZ010000021.1  62514830-62514919   c
            789-866             JAXUCZ010000021.1  62504015-62504092   c
            867-943             JAXUCZ010000021.1  62498829-62498905   c
            944-1045            JAXUCZ010000021.1  62497336-62497437   c
            1046-1156           JAXUCZ010000021.1  62496680-62496790   c
            1157-1270           JAXUCZ010000021.1  62492299-62492412   c
            1271-1410           JAXUCZ010000021.1  62484540-62484679   c
            1411-2078           JAXUCZ010000021.1  62480535-62481202   c
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq22"
     gene            1..2078
                     /gene="Pdk3"
                     /note="pyruvate dehydrogenase kinase 3"
                     /db_xref="GeneID:296849"
                     /db_xref="RGD:1593276"
     exon            1..299
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..1441
                     /gene="Pdk3"
                     /EC_number="2.7.11.2"
                     /note="pyruvate dehydrogenase kinase, isoenzyme 3;
                     [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3,
                     mitochondrial"
                     /codon_start=1
                     /product="pyruvate dehydrogenase kinase, isozyme 3"
                     /protein_id="NP_001100051.1"
                     /db_xref="GeneID:296849"
                     /db_xref="RGD:1593276"
                     /translation="MRLFYRLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKT
                     SYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDP
                     RVLDNFLHVLINIRNRHNDVVPTMAQGVIEYKEKFGFDPFISSNIQYFLDRFYTNRIS
                     FRMLINQHTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPEL
                     EVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDKKEGYPAVKTLVTL
                     GKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLY
                     ARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNP
                     SSEPRDASKYKAKQDKIKSNKTF"
     exon            300..441
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            442..513
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            514..698
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            699..788
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            789..866
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            867..943
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1045
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1156
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1270
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1410
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..2078
                     /gene="Pdk3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccggccacg ggcgcctaac tgctgcccgg tgcttgggta ggtgccgcgg cggcgaccgg
       61 ctactggcgc caaggccgag ccccaggtag aggtaaagcc ccgccggcgc cgcctcctcc
      121 ttgtgtcttg gcggcggcgg cctctgctcc agtccgggac ggcttggcgg atctgcacac
      181 tccaccagta aggatgcggc tcttctaccg gctgctcaag cagccggtcc ccaagcagat
      241 cgagcgctac tcccgcttct ctccgtcgcc gctctctatc aaacagttcc tggacttcgg
      301 aagggataat gcatgtgaaa aaacttcata catgtttctg cgcaaggagc ttcctgtgcg
      361 actggctaac accatgagag aggttaatct tttgccggat aacttgttga accgcccatc
      421 agtgggatta gttcagagtt ggtacatgca gagcttcctt gaacttttag aatatgaaaa
      481 caagagccca gaagatccac gcgttttaga taactttctc cacgttttga taaatatcag
      541 aaacagacac aatgacgttg ttcctacaat ggcccaaggc gtgattgagt acaaggaaaa
      601 gttcgggttt gatccattca ttagcagtaa cattcaatat ttcctggatc ggttttatac
      661 caaccgcatt tctttccgca tgcttattaa ccagcacacc cttctgtttg gtggtgacac
      721 taaccccgct catccgaaac atatagggag tatcgatccc acctgtaatg tagctgatgt
      781 ggtgaaagat gcatatgaaa cagccaagat gctttgtgaa cagtattacc tggtagctcc
      841 agaactggaa gttgaagaat tcaatgccaa agcaccaaac aaacctattc aggtagttta
      901 tgtgccgtca catctgtttc acatgctatt tgagttgttc aagaactcga tgagagcaac
      961 agttgaacta tatgaagata aaaaagaagg ctacccagct gttaaaactc ttgttactct
     1021 gggtaaagaa gacttgtcca ttaagataag tgacctaggt ggtggagtcc cacttcgaaa
     1081 aatagaccgt ctttttaact acatgtactc aactgctcct cgtcccagcc tggagcccac
     1141 aagagctgct cccttggctg gatttggtta tggcttgcca atttctcgac tgtatgccag
     1201 atattttcag ggagatctaa aactgtattc catggaagga gtgggtaccg atgctgtcat
     1261 ttatttgaag gccctttcaa gtgaatcctt tgagaggctg ccagttttca ataagtctgc
     1321 atggcgccat tacaagacca ctcctgaagc tgatgactgg agcaatccca gcagcgaacc
     1381 aagggatgca tcaaaataca aggctaaaca ggacaagatc aagagtaata aaactttcta
     1441 gaaaactgag tgctgtggtc atctctgaag aaagattacc ttctgcaagt caaccagaga
     1501 cccgtatttc taccaactct taaccgtggc actattgtta ttcccaatgc ttagttgctt
     1561 cctgtatatt ggctgagtat gtccatggag ccctccctgt agttagtccc attgtatctt
     1621 ccactagagg tgtagcttga gcaatttttc atgctgtggc atcattagca cccatcacaa
     1681 cacagcacag agcctcctag cgctcttgta ccctatactc tgtatcttgt tatggagttt
     1741 agtgtctgaa gtcttcccag gattctgagc tccttcctca ttccagatcc agcaggtcct
     1801 actgttgctg tttatgacca aatctccagc tagggcttga ggaatctaaa agtgggaggc
     1861 atcagtggag ctcatcttga caattcagtc ccagggttct tcctgtccca tagacatctc
     1921 ccttcattct tgatttggac ctttgccccc ttccgtctga ccggattctc attgggaggt
     1981 gtggtacttg ggtgttagaa cctattcatc tgtatgaatt ttactttacc attcattggc
     2041 ttacttctct tccattgatt aaagcactct aaacgttg
//
