LOCUS       NM_030872               2205 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus pyruvate dehydrogenase kinase 2 (Pdk2), mRNA.
ACCESSION   NM_030872 XM_001081306
VERSION     NM_030872.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Rahman,M.H., Jha,M.K., Kim,J.H., Nam,Y., Lee,M.G., Go,Y.,
            Harris,R.A., Park,D.H., Kook,H., Lee,I.K. and Suk,K.
  TITLE     Pyruvate Dehydrogenase Kinase-mediated Glycolytic Metabolic Shift
            in the Dorsal Root Ganglion Drives Painful Diabetic Neuropathy
  JOURNAL   J Biol Chem 291 (11), 6011-6025 (2016)
   PUBMED   26769971
  REMARK    GeneRIF: PDK2/4 induction and the subsequent lactate surge induce
            the metabolic shift in the diabetic dorsal root ganglion thereby
            contributing to the pathogenesis of painful diabetic neuropathy.
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Wang,J., Kumaran,S., Zhou,J., Nemeria,N.S., Tao,H., Kakalis,L.,
            Park,Y.H., Birkaya,B., Patel,M.S. and Jordan,F.
  TITLE     Elucidation of the interaction loci of the human pyruvate
            dehydrogenase complex E2.E3BP core with pyruvate dehydrogenase
            kinase 1 and kinase 2 by H/D exchange mass spectrometry and nuclear
            magnetic resonance
  JOURNAL   Biochemistry 54 (1), 69-82 (2015)
   PUBMED   25436986
  REMARK    GeneRIF: The Pdk2 and Pdk1 interaction site was identified with
            nuclear magnetic resonance and mass spectrometry.
REFERENCE   3  (bases 1 to 2205)
  AUTHORS   Takao,D., Nemoto,T., Abe,T., Kiyonari,H., Kajiura-Kobayashi,H.,
            Shiratori,H. and Nonaka,S.
  TITLE     Asymmetric distribution of dynamic calcium signals in the node of
            mouse embryo during left-right axis formation
  JOURNAL   Dev Biol 376 (1), 23-30 (2013)
   PUBMED   23357539
REFERENCE   4  (bases 1 to 2205)
  AUTHORS   Hurd,T.R., Collins,Y., Abakumova,I., Chouchani,E.T., Baranowski,B.,
            Fearnley,I.M., Prime,T.A., Murphy,M.P. and James,A.M.
  TITLE     Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial
            reactive oxygen species
  JOURNAL   J Biol Chem 287 (42), 35153-35160 (2012)
   PUBMED   22910903
  REMARK    GeneRIF: Inactivation of pyruvate dehydrogenase kinase 2 by
            mitochondrial reactive oxygen species.
REFERENCE   5  (bases 1 to 2205)
  AUTHORS   Jeoung,N.H., Rahimi,Y., Wu,P., Lee,W.N. and Harris,R.A.
  TITLE     Fasting induces ketoacidosis and hypothermia in
            PDHK2/PDHK4-double-knockout mice
  JOURNAL   Biochem J 443 (3), 829-839 (2012)
   PUBMED   22360721
REFERENCE   6  (bases 1 to 2205)
  AUTHORS   Korotchkina,L.G. and Patel,M.S.
  TITLE     Site specificity of four pyruvate dehydrogenase kinase isoenzymes
            toward the three phosphorylation sites of human pyruvate
            dehydrogenase
  JOURNAL   J Biol Chem 276 (40), 37223-37229 (2001)
   PUBMED   11486000
REFERENCE   7  (bases 1 to 2205)
  AUTHORS   Kolobova,E., Tuganova,A., Boulatnikov,I. and Popov,K.M.
  TITLE     Regulation of pyruvate dehydrogenase activity through
            phosphorylation at multiple sites
  JOURNAL   Biochem J 358 (Pt 1), 69-77 (2001)
   PUBMED   11485553
REFERENCE   8  (bases 1 to 2205)
  AUTHORS   Sugden,M.C., Kraus,A., Harris,R.A. and Holness,M.J.
  TITLE     Fibre-type specific modification of the activity and regulation of
            skeletal muscle pyruvate dehydrogenase kinase (PDK) by prolonged
            starvation and refeeding is associated with targeted regulation of
            PDK isoenzyme 4 expression
  JOURNAL   Biochem J 346 Pt 3 (Pt 3), 651-657 (2000)
   PUBMED   10698691
REFERENCE   9  (bases 1 to 2205)
  AUTHORS   Bowker-Kinley,M.M., Davis,W.I., Wu,P., Harris,R.A. and Popov,K.M.
  TITLE     Evidence for existence of tissue-specific regulation of the
            mammalian pyruvate dehydrogenase complex
  JOURNAL   Biochem J 329 (Pt 1) (Pt 1), 191-196 (1998)
   PUBMED   9405293
REFERENCE   10 (bases 1 to 2205)
  AUTHORS   Popov,K.M., Kedishvili,N.Y., Zhao,Y., Gudi,R. and Harris,R.A.
  TITLE     Molecular cloning of the p45 subunit of pyruvate dehydrogenase
            kinase
  JOURNAL   J Biol Chem 269 (47), 29720-29724 (1994)
   PUBMED   7961963
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Nov 27, 2020 this sequence version replaced NM_030872.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U10357.1, BC061823.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               JAXUCZ010000010.1  80483697-80483912   c
            217-358             JAXUCZ010000010.1  80481944-80482085   c
            359-430             JAXUCZ010000010.1  80475238-80475309   c
            431-615             JAXUCZ010000010.1  80474597-80474781   c
            616-705             JAXUCZ010000010.1  80473078-80473167   c
            706-783             JAXUCZ010000010.1  80472795-80472872   c
            784-860             JAXUCZ010000010.1  80471942-80472018   c
            861-959             JAXUCZ010000010.1  80471726-80471824   c
            960-1067            JAXUCZ010000010.1  80471510-80471617   c
            1068-1181           JAXUCZ010000010.1  80470936-80471049   c
            1182-2205           JAXUCZ010000010.1  80469391-80470414   c
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q26"
     gene            1..2205
                     /gene="Pdk2"
                     /note="pyruvate dehydrogenase kinase 2"
                     /db_xref="GeneID:81530"
                     /db_xref="RGD:69428"
     exon            1..216
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1322
                     /gene="Pdk2"
                     /EC_number="2.7.11.2"
                     /note="[Pyruvate dehydrogenase [lipoamide]] kinase isozyme
                     2, mitochondrial; PDK P45; pyruvate dehydrogenase 2;
                     pyruvate dehydrogenase kinase, isoenzyme 2; PDH kinase 2;
                     [Pyruvate dehydrogenase (acetyl-transferring)] kinase
                     isozyme 2, mitochondrial"
                     /codon_start=1
                     /product="pyruvate dehydrogenase kinase, isozyme 2"
                     /protein_id="NP_110499.1"
                     /db_xref="GeneID:81530"
                     /db_xref="RGD:69428"
                     /translation="MRWFRALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNA
                     CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKD
                     PEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYL
                     SRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCSVSDVVKDAYDMAKLLCDKYYMA
                     SPDLEIQEVNATNATQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLTLPPIKIM
                     VALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISR
                     LYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWC
                     VPSTEPKNTSTYRVS"
     exon            217..358
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            359..430
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            431..615
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            616..705
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            706..783
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            784..860
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            861..959
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1067
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1181
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..2205
                     /gene="Pdk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtctcccggc tgtgcttggc cgtgcggagg gccggtgcca gcacctccag ctccgggaca
       61 gcagcgggag ccaagcccga gccgcaggcg tcgtcgccat gcgctggttc cgggcgctgt
      121 tgaagaatgc gtccctggca ggggcgccca agtacatcga gcacttcagc aagttctccc
      181 cgtccccgct gtccatgaag cagtttctag acttcggatc cagcaatgcc tgcgagaaaa
      241 cttcattcac cttcctccgg caggagctgc ccgtgcgcct ggccaacatc atgaaagaga
      301 tcaacctgct tcctgaccgg gtgctgagca ccccctcagt gcaactggtg cagagctggt
      361 atgtccagag tctgctggac atcatggaat tcctggacaa ggaccccgag gaccaccgga
      421 ccctaagcca gttcactgat gccctggtca ccatccggaa ccggcacaat gacgtagtgc
      481 ccaccatggc acagggagtg ttggagtaca aggacaccta tggtgatgac ccagtctcca
      541 accagaacat ccagtacttt ttggaccgct tctacctcag ccgcatctct atccgcatgc
      601 tcattaacca gcacaccctc atctttgatg gcagcaccaa cccagcccac cccaaacaca
      661 ttggcagcat tgatcccaac tgcagcgtgt ctgatgtggt gaaagatgcc tatgacatgg
      721 ctaagctcct gtgtgacaag tattacatgg cttcccctga cctggagatc caggaagtca
      781 atgccaccaa cgccacccag cccattcaca tggtctacgt cccctcccac ctctaccaca
      841 tgctctttga actcttcaag aatgccatgc gggccacagt ggaaagccac gagtccagcc
      901 tcactctccc tcccatcaaa atcatggtgg ccctcggtga agaagatctg tccatcaaaa
      961 tgagtgaccg aggcgggggt gtccccttga ggaagatcga gaggctcttc agctacatgt
     1021 actctacagc tcctacaccc cagcctggca ctgggggtac cccgctggct ggctttgggt
     1081 atggactccc catttcccgc ctctacgcca agtacttcca gggggacttg cagctcttct
     1141 ctatggaggg ctttgggaca gatgctgtca tctatctgaa ggccctgtcc acggactcag
     1201 tggagcgcct gcctgtctac aacaagtctg cctggcgcca ctaccagacc atccaggagg
     1261 ccggtgactg gtgcgtgccc agcacagagc ccaagaacac atcgacgtat cgggtcagct
     1321 aggggccttc tcttcctggc acctgggagg atgctgccac ctctgaatcc agccaccaca
     1381 gggacttccc tatctatccc ctggggtacg ggggtgaaac tgggtctccc cgatggccag
     1441 atctgtcttt gtagaaatcg cagtggccca tctgtggcga tccctaagtg ccaatctgtc
     1501 tctatggaga aacctagggg gtttccctgg agcctggtct ccatggtgat gatgcttgag
     1561 ggttggggac ggctctacct ggtggggtgc cccagagaca cttctcccaa gaccagagct
     1621 gtctgttttc taccagaaac cctgggtccc cctcactgcc tgcatagtcc tggtctccca
     1681 cgtggctgcc tcgcttgcct tatgcccaca ccctgtacag gcacattggg ctggtttctt
     1741 cgtcagtagt aagaaagatg gagagagact ggggaaacgg gggccaacct tgtctctggt
     1801 cctgcagcct ctctccatct ccactctgga cactaaagtt gccactggga acttgagaat
     1861 gggtggccgt tctcacccaa ggcccaccga gaaggcctaa gagtaacctg tccccaaggc
     1921 gatcttagca atgtttctgc cgcttcctgg cctggcatgt cctcacgtgt atacctcccc
     1981 tgcccagtct acgctcaccc tatccctgct tgagctttag accccagact tcctatgccc
     2041 actatgtgtg cacagacgac tcaaacccag gatgccccat gtacatagcc agttttgtaa
     2101 tctcagatgc ctcacccttg ccctccgcac acaggggtta aagccgtgtg cccctcccag
     2161 tggctgggat ggtgacagtg acatccacag taaatagatg aaatg
//
