LOCUS       NM_053826               1609 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), mRNA.
ACCESSION   NM_053826 XM_001059583 XM_001059645
VERSION     NM_053826.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Kociper,B., Skorja Milic,N., Ogrizek,I., Mis,K. and Pirkmajer,S.
  TITLE     Inhibition of the ubiquitin-proteasome system reduces the abundance
            of pyruvate dehydrogenase kinase 1 in cultured myotubes
  JOURNAL   J Muscle Res Cell Motil 45 (3), 155-169 (2024)
   PUBMED   39080182
  REMARK    GeneRIF: Inhibition of the ubiquitin-proteasome system reduces the
            abundance of pyruvate dehydrogenase kinase 1 in cultured myotubes.
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1609)
  AUTHORS   Cilleros-Mane,V., Just-Borras,L., Polishchuk,A., Duran,M.,
            Tomas,M., Garcia,N., Tomas,J.M. and Lanuza,M.A.
  TITLE     M1 and M2 mAChRs activate PDK1 and regulate PKC betaI and epsilon
            and the exocytotic apparatus at the NMJ
  JOURNAL   FASEB J 35 (7), e21724 (2021)
   PUBMED   34133802
REFERENCE   4  (bases 1 to 1609)
  AUTHORS   Tian,L., Wu,D., Dasgupta,A., Chen,K.H., Mewburn,J., Potus,F.,
            Lima,P.D.A., Hong,Z., Zhao,Y.Y., Hindmarch,C.C.T., Kutty,S.,
            Provencher,S., Bonnet,S., Sutendra,G. and Archer,S.L.
  TITLE     Epigenetic Metabolic Reprogramming of Right Ventricular Fibroblasts
            in Pulmonary Arterial Hypertension: A Pyruvate Dehydrogenase
            Kinase-Dependent Shift in Mitochondrial Metabolism Promotes Right
            Ventricular Fibrosis
  JOURNAL   Circ Res 126 (12), 1723-1745 (2020)
   PUBMED   32216531
  REMARK    GeneRIF: Epigenetic Metabolic Reprogramming of Right Ventricular
            Fibroblasts in Pulmonary Arterial Hypertension: A Pyruvate
            Dehydrogenase Kinase-Dependent Shift in Mitochondrial Metabolism
            Promotes Right Ventricular Fibrosis.
REFERENCE   5  (bases 1 to 1609)
  AUTHORS   Wang,T., Chen,H., Lv,K., Ji,G., Liang,F., Zhang,Y., Wang,Y.,
            Liu,X., Cao,H., Kan,G., Xiong,J., Li,Y. and Qu,L.
  TITLE     Activation of HIF-1alpha and its downstream targets in rat
            hippocampus after long-term simulated microgravity exposure
  JOURNAL   Biochem Biophys Res Commun 485 (3), 591-597 (2017)
   PUBMED   27988334
  REMARK    GeneRIF: The expression of three well-defined downstream targets of
            hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate
            dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and
            vascular endothelial growth factor (VEGF), were up-regulated in
            hippocampus after 28 days of simulated microgravity exposure.
REFERENCE   6  (bases 1 to 1609)
  AUTHORS   Korotchkina,L.G. and Patel,M.S.
  TITLE     Site specificity of four pyruvate dehydrogenase kinase isoenzymes
            toward the three phosphorylation sites of human pyruvate
            dehydrogenase
  JOURNAL   J Biol Chem 276 (40), 37223-37229 (2001)
   PUBMED   11486000
REFERENCE   7  (bases 1 to 1609)
  AUTHORS   Kolobova,E., Tuganova,A., Boulatnikov,I. and Popov,K.M.
  TITLE     Regulation of pyruvate dehydrogenase activity through
            phosphorylation at multiple sites
  JOURNAL   Biochem J 358 (Pt 1), 69-77 (2001)
   PUBMED   11485553
REFERENCE   8  (bases 1 to 1609)
  AUTHORS   Bowker-Kinley,M.M., Davis,W.I., Wu,P., Harris,R.A. and Popov,K.M.
  TITLE     Evidence for existence of tissue-specific regulation of the
            mammalian pyruvate dehydrogenase complex
  JOURNAL   Biochem J 329 (Pt 1) (Pt 1), 191-196 (1998)
   PUBMED   9405293
REFERENCE   9  (bases 1 to 1609)
  AUTHORS   Gudi,R., Bowker-Kinley,M.M., Kedishvili,N.Y., Zhao,Y. and
            Popov,K.M.
  TITLE     Diversity of the pyruvate dehydrogenase kinase gene family in
            humans
  JOURNAL   J Biol Chem 270 (48), 28989-28994 (1995)
   PUBMED   7499431
  REMARK    Erratum:[J Biol Chem 1996 Jan 12;271(2):1250]
REFERENCE   10 (bases 1 to 1609)
  AUTHORS   Popov,K.M., Kedishvili,N.Y., Zhao,Y., Shimomura,Y., Crabb,D.W. and
            Harris,R.A.
  TITLE     Primary structure of pyruvate dehydrogenase kinase establishes a
            new family of eukaryotic protein kinases
  JOURNAL   J Biol Chem 268 (35), 26602-26606 (1993)
   PUBMED   8253790
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC089783.1.
            
            On or before Jun 25, 2006 this sequence version replaced
            XM_001059645.1, XM_001059583.1, NM_053826.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089783.1, L22294.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q23"
     gene            1..1609
                     /gene="Pdk1"
                     /note="pyruvate dehydrogenase kinase 1"
                     /db_xref="GeneID:116551"
                     /db_xref="RGD:69427"
     exon            1..300
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1409
                     /gene="Pdk1"
                     /EC_number="2.7.11.2"
                     /note="[Pyruvate dehydrogenase [lipoamide]] kinase isozyme
                     1, mitochondrial; PDK p48; pyruvate dehydrogenase kinase,
                     isoenzyme 1; PDH kinase 1; [Pyruvate dehydrogenase
                     (acetyl-transferring)] kinase isozyme 1, mitochondrial;
                     pyruvate dehydrogenase kinase, isozyme 1"
                     /codon_start=1
                     /product="pyruvate dehydrogenase kinase, isozyme 1
                     precursor"
                     /protein_id="NP_446278.2"
                     /db_xref="GeneID:116551"
                     /db_xref="RGD:69427"
                     /translation="MRLARLLRGGTSVRPLCAVPCASRSLASDSASGSGPASESGVPG
                     QVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLL
                     RTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGV
                     TEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSIN
                     PNCDVVEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVF
                     ELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMY
                     STAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALST
                     ESIERLPVYNKAAWKHYRTNHEADDWCVPSREPKDMTTFRSS"
     exon            301..442
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            443..514
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            515..699
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            700..789
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            790..867
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            868..944
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1043
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1154
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1268
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1581
                     /gene="Pdk1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcggcccg ccgcggcccg ccacgtcccg cacgtagagc ccgggcagcg gcatagagcg
       61 gcaggttggc gccgggagct ccggctcctc cggcggggct cggtatgagg ctggcaaggc
      121 tgctgcgggg cggcacgagc gtcaggccgc tctgtgccgt cccctgcgcc agccgtagtc
      181 tggcctcgga ctcggcctcg ggctccgggc cggcgtcgga gagcggcgtt ccgggccagg
      241 tggacttcta tgcgcgcttc tcgccgtcgc cactctccat gaagcagttc ctggacttcg
      301 ggtcagtaaa tgcttgtgaa aagacctcgt tcatgtttct tcgacaagag ttgcctgtta
      361 gattggcaaa tataatgaaa gaaataagtc tccttcccga taatcttctc aggaccccat
      421 ccgtacagct ggtacaaagt tggtatatcc aaagccttca ggagctgctt gactttaagg
      481 acaagagtgc tgaggatgct aaaactattt atgaattcac agacacagtg ataaggatca
      541 gaaaccggca caatgatgtc atccccacca tggcccaggg tgtgactgaa tacaaggaga
      601 gcttcggggt ggatcctgtc accagccaaa atgttcagta ctttttggat cggttctaca
      661 tgagtcgcat ctcaattaga atgctactca accagcactc tttattgttt ggtggaaaag
      721 gaagtccatc tcatcgaaaa cacattggaa gcataaatcc aaactgtgat gtagtcgagg
      781 ttattaaaga tggctatgag aatgcgagac ggctttgtga tttgtattac attaactctc
      841 ctgaactaga acttgaagaa ctaaatgcga aatcaccagg acagccaata caagtggttt
      901 atgttccgtc ccatctctat cacatggtgt ttgaactgtt caagaacgca atgagggcca
      961 ccatggagca ccatgcagac aaaggcgttt atcccccgat tcaagttcac gtcacactgg
     1021 gggaggagga tttgactgtg aagatgagtg accggggagg tggtgttccg ctgaggaaga
     1081 tcgacagact cttcaactac atgtactcaa ctgcaccccg gcctcgtgtt gagacgtccc
     1141 gtgcggtgcc cctggctgga tttggttacg gattgcccat atcacgcctc tatgcacagt
     1201 acttccaggg agacctaaag ctgtattcct tagagggcta cgggacggat gctgtcatct
     1261 atattaaggc tctgtcaacg gagtccatcg aaagactccc tgtgtataat aaagctgcct
     1321 ggaagcatta cagaaccaac cacgaggctg acgactggtg tgtgcccagc agagagccga
     1381 aagacatgac gacgttccga agctcgtaac tgcacatggg acgcctggca aagccaaatg
     1441 tggtttttgc caaacttgga agagagatgt atttggaacc acattgtacc aaatatgtca
     1501 tgtgtgattt ttcacaatca gtgtttgcta aagctcctaa aggatcaatt atagcaactt
     1561 tattctgata tgttttgtta aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
