LOCUS       NM_001394536            1761 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus pyruvate dehydrogenase complex, component X
            (Pdhx), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001394536
VERSION     NM_001394536.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Veitch,Z., Zer,A., Loong,H., Salah,S., Masood,M., Gupta,A.,
            Bradbury,P.A., Hogg,D., Wong,A., Kandel,R., Charames,G.S. and Abdul
            Razak,A.R.
  TITLE     A phase II study of ENMD-2076 in advanced soft tissue sarcoma (STS)
  JOURNAL   Sci Rep 9 (1), 7390 (2019)
   PUBMED   31089155
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1761)
  AUTHORS   Mathias,R.A., Greco,T.M., Oberstein,A., Budayeva,H.G.,
            Chakrabarti,R., Rowland,E.A., Kang,Y., Shenk,T. and Cristea,I.M.
  TITLE     Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase
            complex activity
  JOURNAL   Cell 159 (7), 1615-1625 (2014)
   PUBMED   25525879
REFERENCE   4  (bases 1 to 1761)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   5  (bases 1 to 1761)
  AUTHORS   Patel,M.S. and Korotchkina,L.G.
  TITLE     Regulation of mammalian pyruvate dehydrogenase complex by
            phosphorylation: complexity of multiple phosphorylation sites and
            kinases
  JOURNAL   Exp Mol Med 33 (4), 191-197 (2001)
   PUBMED   11795479
  REMARK    Review article
REFERENCE   6  (bases 1 to 1761)
  AUTHORS   Harris,R.A., Bowker-Kinley,M.M., Wu,P., Jeng,J. and Popov,K.M.
  TITLE     Dihydrolipoamide dehydrogenase-binding protein of the human
            pyruvate dehydrogenase complex. DNA-derived amino acid sequence,
            expression, and reconstitution of the pyruvate dehydrogenase
            complex
  JOURNAL   J Biol Chem 272 (32), 19746-19751 (1997)
   PUBMED   9242632
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ232219.1, SRR26360181.931502.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760434 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               JAXUCZ010000003.1  109886547-109886734 c
            189-266             JAXUCZ010000003.1  109865863-109865940 c
            267-367             JAXUCZ010000003.1  109853379-109853479 c
            368-573             JAXUCZ010000003.1  109849254-109849459 c
            574-672             JAXUCZ010000003.1  109848146-109848244 c
            673-847             JAXUCZ010000003.1  109841934-109842108 c
            848-995             JAXUCZ010000003.1  109840279-109840426 c
            996-1054            JAXUCZ010000003.1  109836759-109836817 c
            1055-1213           JAXUCZ010000003.1  109834786-109834944 c
            1214-1278           JAXUCZ010000003.1  109830221-109830285 c
            1279-1761           JAXUCZ010000003.1  109827209-109827691 c
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q32"
     gene            1..1761
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /note="pyruvate dehydrogenase complex, component X"
                     /db_xref="GeneID:311254"
                     /db_xref="RGD:1566332"
     exon            1..188
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1537
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /codon_start=1
                     /product="pyruvate dehydrogenase protein X component,
                     mitochondrial"
                     /protein_id="NP_001381465.1"
                     /db_xref="GeneID:311254"
                     /db_xref="RGD:1566332"
                     /translation="MAASWRLRCNLPLVRYFLGFGSGRSLGLSQGAAAWTVGRGANWR
                     WFHGTQLLQDPIKILMPSLSPTMERGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLD
                     ANDDGILAKIVVEEGAKNIKLGSLIALMVEEGEDWKHVEIPKDVSAPPPVPKPPAPPQ
                     PSPQTQTPCPARKERTVGTPPRLRLSPAARNILEKHSLDASQGTATGPRGVFTKEDAL
                     RLVELKQMGKIAEFRPAPGPPSTLSAPVPPQPTAGLSYPRPMIPPVSIPGQPNAAGTF
                     TEIPASNIRKVIAKRLTESKSTVPHAYATANCDLGAVLKVRRDLVKDDIKVSVNDFII
                     RAAAVTLKQMPGVNVTWDGEGPKHLPSVDISVAVATDKGLITPIIKDAAAKDIREIAD
                     AVKVLSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVL
                     KLTEDEEGNPQVRQHQLITVTMSSDSRMVDDELATKFLETFKANLENPMRLA"
     exon            189..266
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            267..367
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            368..573
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            574..672
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            673..847
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            848..995
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1054
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1213
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1278
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1761
                     /gene="Pdhx"
                     /gene_synonym="Ac1164"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgagctttc ccggagaaag ccgggaagat ggcggcctcc tggaggcttc gctgtaacct
       61 gccgctggta cgttacttcc tgggcttcgg cagcggtcga agcctgggac tgtctcaagg
      121 tgctgctgca tggactgtcg gccgtggagc gaattggagg tggtttcacg gtactcagct
      181 gctgcaggat cctattaaaa tcctaatgcc atcactgtct cctacgatgg aacgagggaa
      241 catcgtgaaa tggctgaaaa aggaaggtga agctgtgagt gctggagacg cgctgtgtga
      301 aattgagact gacaaggctg tggtcacctt agatgcaaat gacgatggga tcttagccaa
      361 gatagtggtt gaagaaggtg ctaaaaatat aaagctgggt tcgctcattg ctttgatggt
      421 tgaagaagga gaagactgga aacacgtgga aattcccaag gatgtcagcg ctccacctcc
      481 agttccaaag ccaccagcgc ctcctcagcc ctctccacag acgcagactc cttgccctgc
      541 caggaaggag cgcacagtag ggacaccacc acggctccgc ttaagtccag ctgcccgcaa
      601 cattctggaa aaacattcat tggacgccag ccagggcaca gccacaggcc ctcgaggggt
      661 cttcactaaa gaagatgcac tcaggttagt cgagctgaaa cagatgggca agattgccga
      721 attcagacca gccccaggtc ctccaagcac tctctcagca cctgtgcctc ctcagcccac
      781 agctgggctg tcgtaccctc ggccaatgat cccaccagtc tctatccctg ggcagcctaa
      841 tgcagcgggt acattcactg aaattcctgc cagcaatatt cgaaaagtta ttgctaagag
      901 gttaactgaa tcaaaaagta ctgtgcctca cgcctatgct actgctaact gtgaccttgg
      961 agcagtttta aaggttagac gagatctggt caaagatgac attaaagtct cagttaatga
     1021 ctttatcatc agagcagcag ctgttactct taagcaaatg cctggggtta atgtcacctg
     1081 ggatggagaa ggcccaaagc atctgccctc tgttgacatt tcagtggctg tggcaacaga
     1141 taaaggttta attacaccaa tcataaaaga tgcagctgct aaggacatac gggagatcgc
     1201 tgacgctgta aaggttctgt caaagaaagc aagagatgga aaattgttgc cggaggagta
     1261 ccaaggagga tcttttagta tttccaactt gggcatgttt ggcatcgatg agttcactgc
     1321 agtgattaac ccgcctcagg cctgtatctt ggcagttgga agattccgtc cagtgctgaa
     1381 actcactgag gatgaagagg ggaaccccca agtgcggcag caccagctta taactgtcac
     1441 gatgtcaagt gacagccgaa tggttgatga tgaactagct accaagtttc ttgaaacttt
     1501 caaagcaaac ttagagaacc ccatgcgact tgcatagcct tcaggaccgg gcgcttggtt
     1561 gggtgttcat atggagcggg tgaagtgcgt atttatttaa ggcgaaagct tttggcctga
     1621 gctgtcttcc tctcccctgt atgtttattg tagaaataaa tgtgtgatga caaactgtga
     1681 ctaataaaga acatttgctt actcagatcc acttttaacc tctggtgctg tacagaaggc
     1741 attaaactga tgtaaatcac a
//
