LOCUS       NM_001007620            1816 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus pyruvate dehydrogenase E1 subunit beta (Pdhb),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001007620 XM_214142
VERSION     NM_001007620.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Mitra,A., Basak,T., Ahmad,S., Datta,K., Datta,R., Sengupta,S. and
            Sarkar,S.
  TITLE     Comparative Proteome Profiling during Cardiac Hypertrophy and
            Myocardial Infarction Reveals Altered Glucose Oxidation by
            Differential Activation of Pyruvate Dehydrogenase E1 Component
            Subunit beta
  JOURNAL   J Mol Biol 427 (11), 2104-2120 (2015)
   PUBMED   25451023
  REMARK    GeneRIF: Phosphorylation of the pyruvate dehydrogenase E1-beta
            leads to a decrease in ventricular ATP level during myocardial
            infarction.
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Wallwiener,M., Wallwiener,C.W., Molinas,R., Rajab,T.K.,
            Brucker,S.Y., Kraemer,B. and Hartkopf,A.D.
  TITLE     Intraabdominal adhesion formation is associated with differential
            mRNA expression of metabolic genes PDHb and SDHa
  JOURNAL   Arch Gynecol Obstet 286 (3), 683-686 (2012)
   PUBMED   22569713
  REMARK    GeneRIF: up-regulated by 23 % in peritoneal adhesions
REFERENCE   3  (bases 1 to 1816)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   4  (bases 1 to 1816)
  AUTHORS   Kato,M., Wynn,R.M., Chuang,J.L., Tso,S.C., Machius,M., Li,J. and
            Chuang,D.T.
  TITLE     Structural basis for inactivation of the human pyruvate
            dehydrogenase complex by phosphorylation: role of disordered
            phosphorylation loops
  JOURNAL   Structure 16 (12), 1849-1859 (2008)
   PUBMED   19081061
REFERENCE   5  (bases 1 to 1816)
  AUTHORS   Okajima,K., Korotchkina,L.G., Prasad,C., Rupar,T., Phillips,J.A.
            3rd, Ficicioglu,C., Hertecant,J., Patel,M.S. and Kerr,D.S.
  TITLE     Mutations of the E1beta subunit gene (PDHB) in four families with
            pyruvate dehydrogenase deficiency
  JOURNAL   Mol Genet Metab 93 (4), 371-380 (2008)
   PUBMED   18164639
REFERENCE   6  (bases 1 to 1816)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 1816)
  AUTHORS   Patel,M.S. and Korotchkina,L.G.
  TITLE     Regulation of mammalian pyruvate dehydrogenase complex by
            phosphorylation: complexity of multiple phosphorylation sites and
            kinases
  JOURNAL   Exp Mol Med 33 (4), 191-197 (2001)
   PUBMED   11795479
  REMARK    Review article
REFERENCE   8  (bases 1 to 1816)
  AUTHORS   Maury,J., Kerbey,A.L., Priestman,D.A., Patel,M.S., Girard,J. and
            Ferre,P.
  TITLE     Pretranslational regulation of pyruvate dehydrogenase complex
            subunits in white adipose tissue during the suckling-weaning
            transition in the rat
  JOURNAL   Biochem J 311 (Pt 2) (Pt 2), 531-535 (1995)
   PUBMED   7487891
REFERENCE   9  (bases 1 to 1816)
  AUTHORS   Matuda,S., Nakano,K., Ohta,S., Saheki,T., Kawanishi,Y. and
            Miyata,T.
  TITLE     The alpha-ketoacid dehydrogenase complexes. Sequence similarity of
            rat pyruvate dehydrogenase with Escherichia coli and Azotobacter
            vinelandii alpha-ketoglutarate dehydrogenase
  JOURNAL   Biochim Biophys Acta 1089 (1), 1-7 (1991)
   PUBMED   2025639
REFERENCE   10 (bases 1 to 1816)
  AUTHORS   Huh,T.L., Casazza,J.P., Huh,J.W., Chi,Y.T. and Song,B.J.
  TITLE     Characterization of two cDNA clones for pyruvate dehydrogenase E1
            beta subunit and its regulation in tricarboxylic acid
            cycle-deficient fibroblast
  JOURNAL   J Biol Chem 265 (22), 13320-13326 (1990)
   PUBMED   2376596
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC079137.1.
            
            On Nov 29, 2004 this sequence version replaced XM_214142.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079137.1, FQ215211.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p14"
     gene            1..1816
                     /gene="Pdhb"
                     /note="pyruvate dehydrogenase E1 subunit beta"
                     /db_xref="GeneID:289950"
                     /db_xref="RGD:1359146"
     exon            1..369
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     CDS             328..1407
                     /gene="Pdhb"
                     /EC_number="1.2.4.1"
                     /note="pyruvate dehydrogenase E1 component subunit beta,
                     mitochondrial; PDHE1-B; pyruvate dehydrogenase (lipoamide)
                     beta; pyruvate dehydrogenase E1 beta subunit"
                     /codon_start=1
                     /product="pyruvate dehydrogenase E1 component subunit
                     beta, mitochondrial precursor"
                     /protein_id="NP_001007621.1"
                     /db_xref="GeneID:289950"
                     /db_xref="RGD:1359146"
                     /translation="MAAVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMD
                     EELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMA
                     GLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPVPIVFRGPNGASAGVAAQHSQ
                     CFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDDNPVVMLENELMYGVAFELPTEAQS
                     KDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDI
                     EAIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPY
                     AKILEDNSIPQVKDIIFAIKKTLNI"
     exon            370..423
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            424..531
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            532..594
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            595..630
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            631..916
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1027
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1119
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1261
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1787
                     /gene="Pdhb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagagaggaa cagcaacctc gcgtcacctc cgcaggacca ggattcgcta gatgggcggg
       61 gctcgcaggc gtcaaaactg gccgttggct acgtatgcag aggcggggcc ataggtctca
      121 ccctgccaaa gcctgggggc ggggcttttg aaatactgcc ctgctattgg cttactcagg
      181 aaggcgggcc tccagttgac attctctatt ggctttgccg aacagaggcg ggacctaaag
      241 gtgtcacgcc ctaccattgg ctgcgccgga cggggtctgc ggcgcttctg tgttgtcatt
      301 cggctgcgag tagaggacac gggcaagatg gcggcggtgg ctggtctggt gcggggacct
      361 ttgcggcagg cttccgggct gctgaagagg cgtttccacc gctcggcgcc cgcggcggtg
      421 cagttgacag ttcgtgaagc cattaatcaa ggtatggatg aagaactaga aagagatgaa
      481 aaggtttttc tgcttgggga agaagttgcc cagtatgatg gtgcatacaa ggttagcaga
      541 ggcctgtgga agaaatatgg tgacaagagg atcatagata ctcccatatc ggagatgggc
      601 tttgctggaa ttgctgttgg tgcagctatg gctgggctgc ggcccatctg tgaatttatg
      661 accttcaatt tctctatgca agctattgac caggtcataa actcagctgc caagacgtac
      721 tatatgtctg ctggcctcca gccggtgccc atagtgttca gggggcccaa cggcgcctca
      781 gcgggcgtgg ctgctcagca ctcgcaatgc ttcgctgcgt ggtatgggca ctgccccggt
      841 ttgaaagtgg tcagcccctg gaattccgag gatgcgaaag gacttattaa atcggccatt
      901 cgtgatgata atccagtggt gatgctagag aatgaactga tgtatggagt tgcatttgaa
      961 cttcccacag aagctcaatc aaaagatttc ctgattccta ttggaaaagc caaaattgaa
     1021 aggcaaggga cccacatcac tgtagttgcc cattcaagac cagtgggcca ctgcctagaa
     1081 gctgcagctg tattgtctaa ggagggaatt gaatgtgagg taataaatct gcggaccatc
     1141 agaccaatgg acattgaagc tatagaagcc agtgtcatga agacaaatca tctcgtaact
     1201 gtggaaggag gctggccaca atttggagta ggagctgaga tttgtgcgag aattatggaa
     1261 ggccctgcat tcaacttcct tgatgcgcct gctgttcgag tcactggtgc tgatgtccct
     1321 atgccttacg caaagattct agaagacaac tccatacctc aggtcaaaga catcatattt
     1381 gcaataaaga agactttgaa tatctaatct agacgttgaa tgtcaagtca gtgaaattta
     1441 tttaaaatac gtgttgggac ttcccctgac gtgtactggg agacttacta gtcaggagtt
     1501 aatgctgatg tggcagcagg tgtctctgta ctggtaagaa gtttaaatgt gcttatgagt
     1561 agaatttttc tactccctac cctagatgct ctgctctctt tgtctgttac tgttaagttg
     1621 gtctagtctt tgaataagat taatataaaa atttgccctc tttcattaga aggggcaaga
     1681 tagaatgccc ccatggaaga taacttatat gtctaaataa aacatatatt atctacattt
     1741 gctgttaaac tgttttgata aggaataaag gggattccta cctatgaaaa aaaaaaaaaa
     1801 aaaaaaaaaa aaaaaa
//
