LOCUS       NM_199268               2426 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus phosphodiesterase 8B (Pde8b), mRNA.
ACCESSION   NM_199268 XM_226661 XM_342176
VERSION     NM_199268.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Tsai,L.C., Chan,G.C., Nangle,S.N., Shimizu-Albergine,M.,
            Jones,G.L., Storm,D.R., Beavo,J.A. and Zweifel,L.S.
  TITLE     Inactivation of Pde8b enhances memory, motor performance, and
            protects against age-induced motor coordination decay
  JOURNAL   Genes Brain Behav 11 (7), 837-847 (2012)
   PUBMED   22925203
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Tsai,L.C., Shimizu-Albergine,M. and Beavo,J.A.
  TITLE     The high-affinity cAMP-specific phosphodiesterase 8B controls
            steroidogenesis in the mouse adrenal gland
  JOURNAL   Mol Pharmacol 79 (4), 639-648 (2011)
   PUBMED   21187369
REFERENCE   3  (bases 1 to 2426)
  AUTHORS   Waddleton,D., Wu,W., Feng,Y., Thompson,C., Wu,M., Zhou,Y.P.,
            Howard,A., Thornberry,N., Li,J. and Mancini,J.A.
  TITLE     Phosphodiesterase 3 and 4 comprise the major cAMP metabolizing
            enzymes responsible for insulin secretion in INS-1 (832/13) cells
            and rat islets
  JOURNAL   Biochem Pharmacol 76 (7), 884-893 (2008)
   PUBMED   18706893
REFERENCE   4  (bases 1 to 2426)
  AUTHORS   Dov,A., Abramovitch,E., Warwar,N. and Nesher,R.
  TITLE     Diminished phosphodiesterase-8B potentiates biphasic insulin
            response to glucose
  JOURNAL   Endocrinology 149 (2), 741-748 (2008)
   PUBMED   17991719
  REMARK    GeneRIF: PDE8B, an 3-isobutyl-1-methylxanthine-insensitive
            cAMP-specific phosphodiesterase, could prove a novel target for
            enhanced insulin response
REFERENCE   5  (bases 1 to 2426)
  AUTHORS   Conti,M. and Beavo,J.
  TITLE     Biochemistry and physiology of cyclic nucleotide
            phosphodiesterases: essential components in cyclic nucleotide
            signaling
  JOURNAL   Annu Rev Biochem 76, 481-511 (2007)
   PUBMED   17376027
  REMARK    Review article
REFERENCE   6  (bases 1 to 2426)
  AUTHORS   Kobayashi,T., Gamanuma,M., Sasaki,T., Yamashita,Y., Yuasa,K.,
            Kotera,J. and Omori,K.
  TITLE     Molecular comparison of rat cyclic nucleotide phosphodiesterase 8
            family: unique expression of PDE8B in rat brain
  JOURNAL   Gene 319, 21-31 (2003)
   PUBMED   14597168
REFERENCE   7  (bases 1 to 2426)
  AUTHORS   Hayashi,M., Matsushima,K., Ohashi,H., Tsunoda,H., Murase,S.,
            Kawarada,Y. and Tanaka,T.
  TITLE     Molecular cloning and characterization of human PDE8B, a novel
            thyroid-specific isozyme of 3',5'-cyclic nucleotide
            phosphodiesterase
  JOURNAL   Biochem Biophys Res Commun 250 (3), 751-756 (1998)
   PUBMED   9784418
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Nov 27, 2020 this sequence version replaced NM_199268.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB092697.1, SRR22582416.520795.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                JAXUCZ010000002.1  28115993-28116051   c
            60-250              JAXUCZ010000002.1  28098396-28098586   c
            251-310             JAXUCZ010000002.1  28095546-28095605   c
            311-368             JAXUCZ010000002.1  28091660-28091717   c
            369-457             JAXUCZ010000002.1  28083163-28083251   c
            458-536             JAXUCZ010000002.1  28077664-28077742   c
            537-677             JAXUCZ010000002.1  28073921-28074061   c
            678-766             JAXUCZ010000002.1  28072344-28072432   c
            767-827             JAXUCZ010000002.1  28070721-28070781   c
            828-870             JAXUCZ010000002.1  28042054-28042096   c
            871-948             JAXUCZ010000002.1  28039944-28040021   c
            949-1025            JAXUCZ010000002.1  28037299-28037375   c
            1026-1190           JAXUCZ010000002.1  28035882-28036046   c
            1191-1236           JAXUCZ010000002.1  28033028-28033073   c
            1237-1372           JAXUCZ010000002.1  28032502-28032637   c
            1373-1571           JAXUCZ010000002.1  28031450-28031648   c
            1572-1789           JAXUCZ010000002.1  28019174-28019391   c
            1790-1910           JAXUCZ010000002.1  28017772-28017892   c
            1911-2078           JAXUCZ010000002.1  28015891-28016058   c
            2079-2208           JAXUCZ010000002.1  28013113-28013242   c
            2209-2426           JAXUCZ010000002.1  28011359-28011576   c
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..2426
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /note="phosphodiesterase 8B"
                     /db_xref="GeneID:309962"
                     /db_xref="RGD:735041"
     exon            1..59
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..2318
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /EC_number="3.1.4.17"
                     /note="cAMP-specific cyclic nucleotide phosphodiesterase
                     PDE8"
                     /codon_start=1
                     /product="high affinity cAMP-specific and IBMX-insensitive
                     3',5'-cyclic phosphodiesterase 8B"
                     /protein_id="NP_954889.1"
                     /db_xref="GeneID:309962"
                     /db_xref="RGD:735041"
                     /translation="MRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESA
                     LECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASV
                     LPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEI
                     TSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQ
                     GVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDSSKQIHRIHRDSGDN
                     SQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVIN
                     IINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRL
                     SGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLV
                     YLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLG
                     KERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTA
                     LAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLA
                     AESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQ
                     TDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADN
                     YKHWKTLDDLKCKSLRLPSNS"
     exon            60..250
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            251..310
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            311..368
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            369..457
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            458..536
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            537..677
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            678..766
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            767..827
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            828..870
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            871..948
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1025
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1190
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1236
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1372
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1571
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1789
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1910
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2078
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2208
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2426
                     /gene="Pde8b"
                     /gene_synonym="RNPDE8B"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggtgtcttc tgccgaggtg cgcattgggc ccatgagact gacacaggac cctatccagg
       61 tcctgctgat ctttgcaaaa gaagatagcc agagcgatgg cttctggtgg gcttgtgaca
      121 gagctggcta cagatgcaac attgctcgaa ctccagagtc ggccctcgaa tgctttctgg
      181 ataagcatca tgagattata gtgatcgatc acaggcagac ccgtaacttt gatgcagaag
      241 ccgtgtgcag gtcaatccgg gccacgaacc cctctgagca cacagtgatc ctggctgtgg
      301 tttcacaagc atctgatgac catgaagagg cctcggtcct tcctcttctc catgcaggct
      361 ttaaccggag atttatggag aacagcagca taattgcttg ctataatgaa ctgattcaaa
      421 tagaacacgg ggaagttcgc tcgcagttca aattacgggc ctgtaactca gtgtttacag
      481 cgttagacca ctgtcacgaa gccatagaaa taacaagtga tgaccacgtg attcagtatg
      541 tcaaccctgc ctttgagagg atgatgggct accacaaagg ggagctcctg gggaaggaac
      601 tcgctgacct acctaagagt gacaagaacc gggcagacct cctggacacc atcaacacgt
      661 gcatcaagaa gggaaaggag tggcaggggg tttactatgc cagacggaaa tccggggaca
      721 gcatccaaca gcacgtgaag atcaccccag tgattggcca aggagggaaa attaggcact
      781 ttgtctccct caagaagctg tgctgtacca ctgacagtag caagcagatc cacaggattc
      841 atcgcgattc cggagacaac tctcagacag agcctcattc gttcagacac aagagcagga
      901 gaaaagagtc cattgatgtg aaatcaatat cttctcgagg cagtgatgcg ccaagcctac
      961 agaatcgccg ctacccctcc atggcaagga tccactccat gaccattgag gcccccatca
     1021 caaaggttat aaatataatc aacgccgctc aagaaaacag cccagttaca gtggcagaag
     1081 ccttggacag agttctagaa attttacgaa ccacagagct gtactccccg cagctgggaa
     1141 ccaaggacga agatcctcac accagtgacc ttgtcggagg cctgatgacc gatggcttga
     1201 ggagactgtc agggaatgag tacgtgttta caaagaatgt gcaccacagt cacagtcacc
     1261 tctcgatgcc catcaccatc aacgatgtcc ccccaagtat tgcccagctc cttgataatg
     1321 aggaaagttg ggacttcaac atctttgaat tggaagctat tacacataaa aggccacttg
     1381 tgtacctggg cttaaaggtc ttctctcggt ttggagtatg tgaattttta aactgtaccg
     1441 aaaccactct tcgggcctgg ctgcaagtga ttgaagcaaa ctaccactcc tccaatgcct
     1501 accacaactc cacccatgcc gccgacgtac tgcacgccac ggctttcttc cttggaaagg
     1561 aaagagtgaa gggaagcctg gaccagctgg atgaggtggc ggctctgatt gcagccacgg
     1621 tgcatgacgt ggatcacccc ggaagaacca actccttcct gtgtaacgca ggcagcgagc
     1681 tcgctgtgct ctacaacgac actgcggtct tggagagtca ccacacagcc ctggccttcc
     1741 agctcaccgt caaggacacc aagtgcaaca ttttcaagaa tatcgacagg aaccactatc
     1801 gaacgctgcg ccaggctatt attgacatgg ttttggccac agagatgaca aaacactttg
     1861 aacatgtgaa caagtttgtg aacagcatca acaagcctct agcagccgag agtgagggca
     1921 gcgactgtga atgcaaccct actgggaaga actttcccga aaaccaaatc ctcatcaagc
     1981 gtatgatgat taagtgtgct gacgtggcca acccgtgccg ccccttggac ctgtgcatcg
     2041 aatgggctgg aaggatctcc gaggagtact ttgcacagac tgacgaagag aagaggcagg
     2101 ggctgcctgt agtgatgcca gtgttcgacc gcaatacctg cagcatcccc aagtcgcaga
     2161 tatccttcat cgactacttc ataacagaca tgtttgatgc ttgggatgcc tttgcacacc
     2221 tgccagccct gatgcaacac ctggccgaca actataaaca ctggaagaca ctggatgacc
     2281 tcaagtgcaa aagtctgaga cttccatcta acagctaaag ccaagccaga gaaggggaca
     2341 ctgacctaca gaggacactg tgaatcggag agtagtacaa gccagagggt ttctcttacg
     2401 gtggaaatga caggcggacg ttaagg
//
