LOCUS       NM_198767               2560 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus phosphodiesterase 8A (Pde8a), mRNA.
ACCESSION   NM_198767 XM_218849
VERSION     NM_198767.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Brown,K.M., Day,J.P., Huston,E., Zimmermann,B., Hampel,K.,
            Christian,F., Romano,D., Terhzaz,S., Lee,L.C., Willis,M.J.,
            Morton,D.B., Beavo,J.A., Shimizu-Albergine,M., Davies,S.A.,
            Kolch,W., Houslay,M.D. and Baillie,G.S.
  TITLE     Phosphodiesterase-8A binds to and regulates Raf-1 kinase
  JOURNAL   Proc Natl Acad Sci U S A 110 (16), E1533-E1542 (2013)
   PUBMED   23509299
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Kruse,L.S., Moller,M. and Kruuse,C.
  TITLE     Distribution of PDE8A in the nervous system of the Sprague-Dawley
            rat
  JOURNAL   J Chem Neuroanat 42 (3), 184-191 (2011)
   PUBMED   21821118
  REMARK    GeneRIF: The neurons exhibiting PDE8A-immunoreactivity were widely
            distributed in the forebrain, brain stem, and cerebellum. Strongly
            immunoreactive neurons were located in the olfactory bulb, the
            septal area, zona incerta, and reticular nucleus of the thalamus.
REFERENCE   3  (bases 1 to 2560)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   4  (bases 1 to 2560)
  AUTHORS   Conti,M. and Beavo,J.
  TITLE     Biochemistry and physiology of cyclic nucleotide
            phosphodiesterases: essential components in cyclic nucleotide
            signaling
  JOURNAL   Annu Rev Biochem 76, 481-511 (2007)
   PUBMED   17376027
  REMARK    Review article
REFERENCE   5  (bases 1 to 2560)
  AUTHORS   Kobayashi,T., Gamanuma,M., Sasaki,T., Yamashita,Y., Yuasa,K.,
            Kotera,J. and Omori,K.
  TITLE     Molecular comparison of rat cyclic nucleotide phosphodiesterase 8
            family: unique expression of PDE8B in rat brain
  JOURNAL   Gene 319, 21-31 (2003)
   PUBMED   14597168
REFERENCE   6  (bases 1 to 2560)
  AUTHORS   Soderling,S.H., Bayuga,S.J. and Beavo,J.A.
  TITLE     Cloning and characterization of a cAMP-specific cyclic nucleotide
            phosphodiesterase
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8991-8996 (1998)
   PUBMED   9671792
REFERENCE   7  (bases 1 to 2560)
  AUTHORS   Fisher,D.A., Smith,J.F., Pillar,J.S., St Denis,S.H. and Cheng,J.B.
  TITLE     Isolation and characterization of PDE8A, a novel human
            cAMP-specific phosphodiesterase
  JOURNAL   Biochem Biophys Res Commun 246 (3), 570-577 (1998)
   PUBMED   9618252
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB092696.1.
            
            On Dec 17, 2003 this sequence version replaced XM_218849.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB092696.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q31"
     gene            1..2560
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /note="phosphodiesterase 8A"
                     /db_xref="GeneID:308776"
                     /db_xref="RGD:735125"
     exon            1..230
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..2534
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /EC_number="3.1.4.17"
                     /note="cAMP-specific cyclic nucleotide phosphodiesterase
                     PDE8"
                     /codon_start=1
                     /product="high affinity cAMP-specific and IBMX-insensitive
                     3',5'-cyclic phosphodiesterase 8A"
                     /protein_id="NP_942062.1"
                     /db_xref="GeneID:308776"
                     /db_xref="RGD:735125"
                     /translation="MGCAPSIHISDNRVSNPSDSDGEDEDVRVPALRSRQRWSTPPGL
                     VEPQPRDSGASKVSVADVQFGPMRFHQDQLQVLLVFTKEDSQCNGFYRACEKAGFKCT
                     VTKEVQTVLACFQDKLHDIIIIDHRNPRQMDAEAMCRSIRSSKLSENTVIVGVVRRVD
                     KEEVSLMPFLAAGFTRRYIENPNVMACYNELLQLAFGEVRSQLKLRACNSVFTALEKS
                     QEAIEITSEDHIIQYANPAFETTMGYQSGELIGKELAQVPINEKKGDLLDAINSCVTI
                     DKEWQGVYHTQKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGSNKVETTTECVQ
                     TDSQTDSQAGKHKDRRKHSMDVKAVSSRTSEVSNQRRHSSLARIHSMMIEAPITKVIN
                     ILSAAQENSPVSVTEALNHVLDILRTTELYSPQFNTEDDPHATDLVGGLMSDGLRRFS
                     GNEYTLATKSLRPSSSQLAVPISLHDIPPRIAQVIENEEQWDFDIFELEAATQNRPLI
                     YLGLKTFARFEICEFLQCSEATLKSWFQIIESNYHSSNPYHNSTHAADVLHATAYFLS
                     RNKLKDTLDRLDEVAALIAATIHDVDHPGRTNSFLCNAGKELAVLYNDTAVLESHHAA
                     LAFQLTLENDKCNIFKNTDRNDYRTLRQSIIDMVLATEMTKHFEHVNKFINSINKPLK
                     AQKNEVQETGRSLEDIRAMVRTPESRTLIKRMMIKCADVSNPCRPLEHCIEWAARISE
                     EYFSQTDEEKQLDLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVYLPNLMR
                     HLEDNFKYWKGLDEKELCTLRPPPE"
     exon            231..287
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            288..478
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            479..535
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            536..590
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            591..679
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            680..758
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            759..896
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            897..985
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1037
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1080
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1158
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1229
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1391
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1440
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1576
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1775
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1993
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2123
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2297
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            2298..2427
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2560
                     /gene="Pde8a"
                     /gene_synonym="RNPDE8A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggccgtttgc tgaccggatc gcggctgcca gccagcgtgt ccgcggtgcc gccgccgcca
       61 ggatgggctg cgccccgagc atccacattt ccgacaaccg agtgtcgaac cccagcgaca
      121 gcgacggcga ggacgaggac gtccgggtcc ccgcgctgag gtctagacag cgatggtcga
      181 ccccgcccgg gctcgtggag cctcagcctc gcgacagcgg tgcctcgaag gtatcagttg
      241 ctgatgtgca atttggcccg atgagatttc atcaagacca gcttcaggta cttttagtgt
      301 ttaccaaaga agatagccag tgtaatggat tctacagggc atgtgaaaaa gccgggttta
      361 agtgcacagt caccaaggag gtgcagactg tccttgcctg tttccaggac aaactccatg
      421 acatcatcat catagaccac agaaatcccc gacagatgga tgcggaggca atgtgcaggt
      481 ctatcagatc atcaaaatta tcagaaaaca cagttattgt tggcgtagta cgcagggtgg
      541 acaaagaaga ggtgtcttta atgcccttcc ttgccgctgg cttcacgcgg aggtatatag
      601 aaaaccccaa cgtcatggcc tgttataatg aactgctcca gttggcattt ggagaggtgc
      661 gatcacagct gaaactcagg gcttgtaact cagtgttcac tgcgttagag aagagtcaag
      721 aagcgattga gatcacaagt gaggaccaca tcatccagta tgcaaaccct gcatttgaaa
      781 caacaatggg ttatcagtcg ggagaactaa tagggaagga gttagcacaa gtgcccatca
      841 atgagaagaa aggtgacttg ctggatgcca taaattcctg tgtcacaatc gacaaggagt
      901 ggcaaggagt ttaccatacc caaaagaaaa atggagataa catccaacaa aatgtgaaga
      961 taatacctgt cattgggcag ggaggaaaaa tcagacacta tgtgtccatt atcagagtgt
     1021 gcaatggcag caataaggtt gagaccacaa ctgagtgtgt tcagactgat agtcaaacag
     1081 atagtcaggc aggcaagcat aaagacagga ggaaacattc gatggacgtc aaagcggttt
     1141 cctccagaac aagcgaagtt tccaatcaga gaagacattc ctctctggcc cggatacatt
     1201 ctatgatgat tgaggcacca atcaccaagg taatcaatat tcttagtgct gcccaggaaa
     1261 acagtcctgt gtctgtgaca gaagccttga accatgtgct ggatatcctg agaaccactg
     1321 aactgtattc accccagttt aatactgagg atgaccctca cgccactgac ctcgttgggg
     1381 gcttaatgtc tgatggctta cggaggtttt caggaaatga gtatactctt gcaacaaaaa
     1441 gccttcggcc atcttcaagc cagttggccg tccccatctc ccttcatgat atcccaccac
     1501 ggattgctca ggtcatagaa aatgaggagc aatgggactt tgacattttt gagctggaag
     1561 ctgcaaccca aaataggcct ttgatttatc taggtctcaa aacgtttgct cgttttgaaa
     1621 tctgtgagtt cttacagtgc tctgaggcaa cgctgaaatc atggtttcaa attattgaat
     1681 ccaattacca ttcctctaac ccctaccaca attccacgca cgccgcggat gtgcttcacg
     1741 ccactgcgta tttcctttcc agaaacaagt taaaggacac tttagatcgc cttgatgagg
     1801 tcgctgccct catcgctgcc accatccacg atgtggatca ccccgggaga accaactctt
     1861 tcctctgcaa tgcgggaaag gagctggccg tactgtacaa tgacactgct gtgctagaga
     1921 gccaccacgc agcgctggcc ttccagctga cccttgaaaa tgacaaatgc aatatcttta
     1981 aaaacacaga caggaatgac taccggacac tacgccagag tatcattgac atggtcctag
     2041 ccacagagat gacaaagcac tttgaacacg tcaacaaatt tatcaacagc atcaacaagc
     2101 ctctgaaagc acagaaaaac gaggttcagg aaactggcag aagtctggaa gatataagag
     2161 ccatggtcag gactccagag agccggactc tcatcaaaag gatgatgatt aagtgtgccg
     2221 acgtgtccaa cccctgccgg cccctcgagc actgcatcga gtgggctgca cgcatctcag
     2281 aggagtattt ctcccagacc gatgaagaga aacagctgga cttgcctgtg gtgatgccag
     2341 tgtttgacag aaacacctgc agcatcccca agtctcagat ctccttcatc gactacttca
     2401 tcacagacat gtttgatgcc tgggatgcct ttgtttacct gcctaacctt atgcggcacc
     2461 tggaggacaa cttcaagtac tggaaaggac tggatgagaa ggagctgtgc accctccggc
     2521 cccctccaga atagtgggag gtacacacta ggccccctgg
//
