LOCUS       NM_133584               3397 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus phosphodiesterase 5A (Pde5a), mRNA.
ACCESSION   NM_133584 XM_001074236 XM_001074257
VERSION     NM_133584.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   He,W., Xiang,H., Liu,D., Liu,J., Li,M., Wang,Q., Qian,Q., Li,Y.,
            Fu,X., Chen,P., Guo,Y., Zeng,G., Wu,Z., Zhan,D., Wang,X.,
            DiSanto,M.E. and Zhang,X.
  TITLE     Changes in the expression and function of the PDE5 pathway in the
            obstructed urinary bladder
  JOURNAL   J Cell Mol Med 24 (22), 13181-13195 (2020)
   PUBMED   33009887
  REMARK    GeneRIF: Changes in the expression and function of the PDE5 pathway
            in the obstructed urinary bladder.
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Kulshrestha,R., Singh,H., Pandey,A., Soundarya,D., Jaggi,A.S. and
            Ravi,K.
  TITLE     Differential expression of caveolin-1 during pathogenesis of
            combined pulmonary fibrosis and emphysema: Effect of
            phosphodiesterase-5 inhibitor
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (8), 165802 (2020)
   PUBMED   32311453
  REMARK    GeneRIF: Differential expression of caveolin-1 during pathogenesis
            of combined pulmonary fibrosis and emphysema: Effect of
            phosphodiesterase-5 inhibitor.
REFERENCE   3  (bases 1 to 3397)
  AUTHORS   Sanguesa,G., Shaligram,S., Akther,F., Roglans,N., Laguna,J.C.,
            Rahimian,R. and Alegret,M.
  TITLE     Type of supplemented simple sugar, not merely calorie intake,
            determines adverse effects on metabolism and aortic function in
            female rats
  JOURNAL   Am J Physiol Heart Circ Physiol 312 (2), H289-H304 (2017)
   PUBMED   27923787
REFERENCE   4  (bases 1 to 3397)
  AUTHORS   Zhang,W., Zang,N., Jiang,Y., Chen,P., Wang,X. and Zhang,X.
  TITLE     Upregulation of Phosphodiesterase type 5 in the Hyperplastic
            Prostate
  JOURNAL   Sci Rep 5, 17888 (2015)
   PUBMED   26657792
  REMARK    GeneRIF: Our data showed a significant and previously undocumented
            upregulation of PDE5 in both rat and human BPH.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3397)
  AUTHORS   West,C.A., Shaw,S., Sasser,J.M., Fekete,A., Alexander,T.,
            Cunningham,M.W. Jr., Masilamani,S.M. and Baylis,C.
  TITLE     Chronic vasodilation increases renal medullary PDE5A and alpha-ENaC
            through independent renin-angiotensin-aldosterone system pathways
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 305 (10), R1133-R1140 (2013)
   PUBMED   24068049
  REMARK    GeneRIF: The effects of vasodilation on plasma volume
            expansionhemodilution, and PDE5A expression via the
            renin-angiotensin-aldosterone system are reported.
REFERENCE   6  (bases 1 to 3397)
  AUTHORS   Bender,A.T., Ostenson,C.L., Giordano,D. and Beavo,J.A.
  TITLE     Differentiation of human monocytes in vitro with
            granulocyte-macrophage colony-stimulating factor and macrophage
            colony-stimulating factor produces distinct changes in cGMP
            phosphodiesterase expression
  JOURNAL   Cell Signal 16 (3), 365-374 (2004)
   PUBMED   14687666
REFERENCE   7  (bases 1 to 3397)
  AUTHORS   Rybalkin,S.D., Rybalkina,I.G., Shimizu-Albergine,M., Tang,X.B. and
            Beavo,J.A.
  TITLE     PDE5 is converted to an activated state upon cGMP binding to the
            GAF A domain
  JOURNAL   EMBO J 22 (3), 469-478 (2003)
   PUBMED   12554648
REFERENCE   8  (bases 1 to 3397)
  AUTHORS   Murray,F., MacLean,M.R. and Pyne,N.J.
  TITLE     Increased expression of the cGMP-inhibited cAMP-specific (PDE3) and
            cGMP binding cGMP-specific (PDE5) phosphodiesterases in models of
            pulmonary hypertension
  JOURNAL   Br J Pharmacol 137 (8), 1187-1194 (2002)
   PUBMED   12466227
  REMARK    GeneRIF: mRNA transcript and protein levels of PDE5 in the main and
            first branch of the pulmonary arteries are increased by chronic
            hypoxia.
REFERENCE   9  (bases 1 to 3397)
  AUTHORS   Kotera,J., Fujishige,K., Imai,Y., Kawai,E., Michibata,H.,
            Akatsuka,H., Yanaka,N. and Omori,K.
  TITLE     Genomic origin and transcriptional regulation of two variants of
            cGMP-binding cGMP-specific phosphodiesterases
  JOURNAL   Eur J Biochem 262 (3), 866-873 (1999)
   PUBMED   10411650
REFERENCE   10 (bases 1 to 3397)
  AUTHORS   Kotera,J., Yanaka,N., Fujishige,K., Imai,Y., Akatsuka,H.,
            Ishizuka,T., Kawashima,K. and Omori,K.
  TITLE     Expression of rat cGMP-binding cGMP-specific phosphodiesterase mRNA
            in Purkinje cell layers during postnatal neuronal development
  JOURNAL   Eur J Biochem 249 (2), 434-442 (1997)
   PUBMED   9370351
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D89093.1.
            
            On or before Jun 25, 2006 this sequence version replaced
            XM_001074236.1, XM_001074257.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D89093.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q42"
     gene            1..3397
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /note="phosphodiesterase 5A"
                     /db_xref="GeneID:171115"
                     /db_xref="RGD:620995"
     exon            1..500
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             475..2976
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /EC_number="3.1.4.35"
                     /note="cGMP-binding cGMP specific phosphodiesterase 5A2;
                     CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase;
                     phosphodiesterase 5A, cGMP-specific; phosphodiesterase
                     type 5"
                     /codon_start=1
                     /product="cGMP-specific 3',5'-cyclic phosphodiesterase"
                     /protein_id="NP_598268.1"
                     /db_xref="GeneID:171115"
                     /db_xref="RGD:620995"
                     /translation="MLPFGDKTRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLPQS
                     PHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTSPRFDSD
                     EGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVS
                     RLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITG
                     YKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQ
                     LYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDC
                     PDSFSRVFQMEWEEVGKSSEPLTREHDANKINYMYAQYVKNTMEPLNIPDVTKDNRFP
                     WTNENMGHINTHCIRSLLCTPIKNGKKNKVIGVCQLVNKMEEKTGKIKAFNQNDEQFL
                     EAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALAAAVVPS
                     AQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRK
                     NVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQ
                     RSEHPLAQLYCHSTMEHHHFDQCLMVLNSPGNQILSGLSIEEYKTTLKIIKQAILATD
                     LALYIKRRGEFFELIRKNEFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAELV
                     AAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCL
                     PLLDGCRKNRQKWQALADQQEKTLLNGESGQAKRD"
     exon            501..1089
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1179
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1251
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1341
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1479
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1547
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1656
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1744
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1920
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..1980
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2127
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2253
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2348
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2436
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2537
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2615
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2679
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2754
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2838
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..3397
                     /gene="Pde5a"
                     /gene_synonym="PDE5A2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagacttga gagtggagcg gagggacgag agggtggagc ggagtgcgtg gcgggcgggt
       61 cccccgaagc tcgaggagtc gccgcgaagc ccgcgcgcgc gcttgcctcc ggcgcgccga
      121 tctgggctga actaacaagc tctgctttgg agaaggggct ggagccccgc caccggcgcc
      181 ttggggcaac ggccgtcgag cagctggtgc tggccgcccg agccgggagg tcgactctcg
      241 ccgggcgggg atggtggacg agccggcggg gactcgcccg cgcgcggccg ccacagggcc
      301 cggcgctgcc cgccaggggg cgacgccacg cggggtccgc agccggcgac ccgcagcgaa
      361 gaggcggcgg gggccataag ggagccggtc gggcacccac ggaactccgc gggcccccag
      421 agtctcccca gtccaccatc cgaggagttc ggaaacgagc gccccacctc tgctatgttg
      481 ccctttggag acaaaacgag agacatggtc aatgcatggt tttcagagag agttcacaac
      541 attcctgtgt gcaaggaagg catccgggcc cacaccgagt cctgctcttg ctctctgcct
      601 caaagtcctc atgcagataa taccacccct ggagcaccag ccagaaaaat ctctgcctct
      661 gaatttgacc gacctcttag acccattgtt gtcaaggatt ctgagggaac cgtgagcttt
      721 ctctctgact caggaaaaaa ggaacagatg ccactaacct ctccaagatt tgatagtgat
      781 gaaggggacc agtgctcaag actcttggaa ctagtaaaag atatttccag tcatttggat
      841 gtcacagcct tgtgtcacaa aattttcttg catatccatg gacttatctc tgctgatcgc
      901 tattccctgt tccttgtctg tgaggacagc tctaaagaca aatttctcgt cagtcgcctc
      961 tttgatgttg ctgaaggttc aacactggag gaagcttcaa ataactgtat ccgtttagaa
     1021 tggaacaaag gcattgtggg acatgtggca gcttttggag agcccttgaa catcaaagat
     1081 gcctatgagg atccccggtt caatgcagaa gttgaccaaa ttacaggcta caagacacaa
     1141 agtatccttt gtatgccaat taagaaccac agggaagagg tcgttggtgt agctcaggcc
     1201 atcaacaaga aatcaggaaa cggtgggaca ttcactgaaa aagatgaaaa ggactttgct
     1261 gcctatctgg cattttgtgg cattgttctt cacaacgccc agctctatga aacgtcactg
     1321 ctggagaaca aaagaaatca ggtattactt gaccttgcta gcttaatttt cgaggagcag
     1381 cagtcattgg aagtaattct gaagaaaata gccgccacta ttatctcctt catgcaggtg
     1441 cagaagtgca ccatcttcat cgtggatgaa gactgccctg actcgttctc tcgtgtgttt
     1501 caaatggagt gggaggaagt agggaaatcc tccgagcctt tgacaaggga gcacgatgca
     1561 aacaaaatca attacatgta tgctcagtat gtcaaaaaca cgatggagcc acttaatatc
     1621 ccagatgtca ccaaggacaa cagatttccc tggacaaatg aaaacatggg ccatataaac
     1681 acgcactgca tcagaagttt gctttgcacg cctataaaaa atgggaagaa gaacaaagtc
     1741 ataggggttt gccaacttgt taataagatg gaggagaaga ctggcaagat taaggctttc
     1801 aaccaaaacg atgaacaatt cctggaggct tttgtcatct tctgtggctt ggggatccag
     1861 aacacacaga tgtacgaagc ggtggagaga gccatggcca agcagatggt gacgttagag
     1921 gtcctgtctt atcacgcatc agcggcggag gaagaaacaa gggagctcca ggctctagcg
     1981 gctgctgtgg taccgtctgc ccaaaccctt aaaattaccg acttcagctt cagtgacttc
     2041 gagctgtctg atctggaaac agcgctgtgt acaattcgga tgttcactga cctcaacctt
     2101 gtgcagaact tccagatgaa acacgaggtt ctttgccgat ggattttgag tgtcaagaag
     2161 aattatcgga agaacgttgc ctaccacaat tggaggcacg cctttaacac ggcacagtgc
     2221 atgtttgctg ctctgaaagc aggcaagatt cagaacaagt tgacggatct ggagacgctt
     2281 gcattgctga ttgccgcttt aagccatgat ttggaccatc gtggtgtgaa caactcatac
     2341 atacagcgga gcgagcaccc cctggcccag ctgtactgcc actccaccat ggagcaccat
     2401 cacttcgacc agtgcttgat ggttctaaac agcccaggca accagatcct cagtggcctc
     2461 tccattgaag aatataagac cacattgaaa ataatcaagc aagcaatttt agccactgac
     2521 ctagcactgt acattaagag acgaggagaa ttttttgaac ttataagaaa aaatgaattc
     2581 agttttgaag atcctcttca aaaggagttg tttctggcaa tgctgatgac tgcctgtgat
     2641 ctctccgcca ttactaagcc ctggcctatt caacaacgga tagcagaact cgtggcagcc
     2701 gaattctttg atcaaggaga cagagagaga aaagaactta acatggagcc cgctgatcta
     2761 atgaacaggg agaagaaaaa taaaatccca agtatgcaag ttgggttcat agacgccatc
     2821 tgcttgcagc tgtatgaggc cctgacccac gtctcagaag actgtttgcc cctgctggat
     2881 ggctgcagga agaacaggca gaaatggcaa gccctcgcag accagcagga gaagacgctc
     2941 cttaatgggg agagcggcca ggccaagcgg gactgacctt ctccccctcc tcccctcccc
     3001 cacccccacc gggtgttcga actgccctag gcctggtttc gccatacact atgcagactc
     3061 gagtgcactt tgccactgct gtattttatt tttgcacaac ttctgagagc atgaggtgtt
     3121 tcagagattg ctacctactt tccgtgcttt tgttttatgc cactgaagtg gagccgtcag
     3181 tcatgcccac tctaacactt tgataagagt ggtgtaaact gcattcccac acgcagcttg
     3241 gttatgcttg gggagtatga ataaccggcc tttgtgtttc tgagtcacca gtctttcaaa
     3301 cacgttagga atcgttctcg gaagtgggtt aggggcgaac tgaatgtgtc ctgggacatt
     3361 taaagccatc ctgaaattaa cgctgagtgg atgttag
//
