LOCUS       NM_017229               5368 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus phosphodiesterase 3B (Pde3b), mRNA.
ACCESSION   NM_017229
VERSION     NM_017229.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,L., Zhang,C., Wu,L., Qin,L. and Liu,T.
  TITLE     Diosgenin reduces phosphodiesterase 3B (PDE3B) through
            AMP-activated protein kinase/ mechanistic target of rapamycin
            (AMPK/mTOR) signaling pathway to ameliorate streptozotocin-induced
            pancreatic beta-cell apoptosis and dysfunction
  JOURNAL   Bioengineered 13 (2), 2217-2225 (2022)
   PUBMED   35030973
  REMARK    GeneRIF: Diosgenin reduces phosphodiesterase 3B (PDE3B) through
            AMP-activated protein kinase/ mechanistic target of rapamycin
            (AMPK/mTOR) signaling pathway to ameliorate streptozotocin-induced
            pancreatic beta-cell apoptosis and dysfunction.
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Ghorbani,A., Omrani,G.R., Hadjzadeh,M.A. and Varedi,M.
  TITLE     Proinsulin C-peptide inhibits lipolysis in diabetic rat adipose
            tissue through phosphodiestrase-3B enzyme
  JOURNAL   Horm Metab Res 45 (3), 221-225 (2013)
   PUBMED   22990990
  REMARK    GeneRIF: C-peptide has the potential to act, conditionally, as an
            antilipolytic hormone by activating phosphodiesterase-3B.
REFERENCE   3  (bases 1 to 5368)
  AUTHORS   Sahu,A., Koshinaka,K. and Sahu,M.
  TITLE     Phosphatidylinositol 3-kinase is an upstream regulator of the
            phosphodiesterase 3B pathway of leptin signalling that may not
            involve activation of Akt in the rat hypothalamus
  JOURNAL   J Neuroendocrinol 25 (2), 168-179 (2013)
   PUBMED   22967108
  REMARK    GeneRIF: PI3K but not Akt is an upstream regulator of the PDE3B
            pathway of leptin signalling in the rat hypothalamus.
REFERENCE   4  (bases 1 to 5368)
  AUTHORS   Baragli,A., Ghe,C., Arnoletti,E., Granata,R., Ghigo,E. and
            Muccioli,G.
  TITLE     Acylated and unacylated ghrelin attenuate isoproterenol-induced
            lipolysis in isolated rat visceral adipocytes through activation of
            phosphoinositide 3-kinase gamma and phosphodiesterase 3B
  JOURNAL   Biochim Biophys Acta 1811 (6), 386-396 (2011)
   PUBMED   21435395
  REMARK    GeneRIF: Data demonstrate that AG/UAG attenuation of ISO-induced
            lipolysis involves PI3Kgamma/AKT and PDE3B.
REFERENCE   5  (bases 1 to 5368)
  AUTHORS   Wilson,L.S., Baillie,G.S., Pritchard,L.M., Umana,B., Terrin,A.,
            Zaccolo,M., Houslay,M.D. and Maurice,D.H.
  TITLE     A phosphodiesterase 3B-based signaling complex integrates exchange
            protein activated by cAMP 1 and phosphatidylinositol 3-kinase
            signals in human arterial endothelial cells
  JOURNAL   J Biol Chem 286 (18), 16285-16296 (2011)
   PUBMED   21393242
REFERENCE   6  (bases 1 to 5368)
  AUTHORS   Nagaoka,T., Shirakawa,T., Balon,T.W., Russell,J.C. and
            Fujita-Yamaguchi,Y.
  TITLE     Cyclic nucleotide phosphodiesterase 3 expression in vivo: evidence
            for tissue-specific expression of phosphodiesterase 3A or 3B mRNA
            and activity in the aorta and adipose tissue of
            atherosclerosis-prone insulin-resistant rats
  JOURNAL   Diabetes 47 (7), 1135-1144 (1998)
   PUBMED   9648839
REFERENCE   7  (bases 1 to 5368)
  AUTHORS   He,R., Komas,N., Ekholm,D., Murata,T., Taira,M., Hockman,S.,
            Degerman,E. and Manganiello,V.C.
  TITLE     Expression and characterization of deletion recombinants of two
            cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE-3)
  JOURNAL   Cell Biochem Biophys 29 (1-2), 89-111 (1998)
   PUBMED   9631240
REFERENCE   8  (bases 1 to 5368)
  AUTHORS   Degerman,E., Belfrage,P. and Manganiello,V.C.
  TITLE     Structure, localization, and regulation of cGMP-inhibited
            phosphodiesterase (PDE3)
  JOURNAL   J Biol Chem 272 (11), 6823-6826 (1997)
   PUBMED   9102399
  REMARK    Review article
REFERENCE   9  (bases 1 to 5368)
  AUTHORS   Manganiello,V.C., Taira,M., Degerman,E. and Belfrage,P.
  TITLE     Type III cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE3
            gene family)
  JOURNAL   Cell Signal 7 (5), 445-455 (1995)
   PUBMED   8562305
  REMARK    Review article
REFERENCE   10 (bases 1 to 5368)
  AUTHORS   Taira,M., Hockman,S.C., Calvo,J.C., Taira,M., Belfrage,P. and
            Manganiello,V.C.
  TITLE     Molecular cloning of the rat adipocyte hormone-sensitive cyclic
            GMP-inhibited cyclic nucleotide phosphodiesterase
  JOURNAL   J Biol Chem 268 (25), 18573-18579 (1993)
   PUBMED   8395509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Dec 3, 2020 this sequence version replaced NM_017229.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z22867.1, SRR26643293.5212.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1228              JAXUCZ010000001.1  178041207-178042434
            1229-1279           JAXUCZ010000001.1  178132644-178132694
            1280-1528           JAXUCZ010000001.1  178134756-178135004
            1529-1668           JAXUCZ010000001.1  178137706-178137845
            1669-1775           JAXUCZ010000001.1  178141726-178141832
            1776-1986           JAXUCZ010000001.1  178166473-178166683
            1987-2060           JAXUCZ010000001.1  178167352-178167425
            2061-2200           JAXUCZ010000001.1  178175109-178175248
            2201-2338           JAXUCZ010000001.1  178176297-178176434
            2339-2450           JAXUCZ010000001.1  178178402-178178513
            2451-2564           JAXUCZ010000001.1  178180152-178180265
            2565-2764           JAXUCZ010000001.1  178185685-178185884
            2765-2968           JAXUCZ010000001.1  178197195-178197398
            2969-3130           JAXUCZ010000001.1  178198907-178199068
            3131-3419           JAXUCZ010000001.1  178201173-178201461
            3420-5368           JAXUCZ010000001.1  178202555-178204503
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q34"
     gene            1..5368
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /note="phosphodiesterase 3B"
                     /db_xref="GeneID:29516"
                     /db_xref="RGD:61943"
     exon            1..1228
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..3634
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /EC_number="3.1.4.17"
                     /note="cGMP-inhibited 3',5'-cyclic phosphodiesterase B;
                     CGIPDE1; CGI-PDE B; cyclic GMP-inhibited phosphodiesterase
                     B; phosphodiesterase 3B, cGMP-inhibited"
                     /codon_start=1
                     /product="cGMP-inhibited 3',5'-cyclic phosphodiesterase
                     3B"
                     /protein_id="NP_058925.2"
                     /db_xref="GeneID:29516"
                     /db_xref="RGD:61943"
                     /translation="MRKDERERDTPAMRSPPPPPPPATATAASPPESLRNGYVKSCVS
                     PLRQDPPRSFFFHLCRFCNVEPPAASLRAGARLSLAALAAFVLAALLGAGPERWAAAA
                     TGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPDRGAGSWWLLALPACCYLGD
                     FAAWQWWSWLRGEPAAAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWVSF
                     SGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTADEKVPVIRPR
                     RRSSCVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNG
                     VDLSVLNEARNMVSDLLIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFY
                     PCSEVEDPVEKGDRKLHKGLSSKPSFPTAQLRRSSGASGLLTSEHHSRWDRSGGKRPY
                     QELSVSSHGCHLNGPFSSNLMTIPKQRSSSVSLTHHAGLRRAGALPSPSLLNSSSHVP
                     VSAGCLTNRSPVGFLDTSDFLTKPSVTLHRSLGSVSSAADFHQYLRNSDSSLCSSCGH
                     QILKYVSTCEPDGTDHHNEKSGEEDSTVFSKERLNIVETQEEETVKEDCRELFLEGDD
                     HLMEEAQQPNIDQEVLLDPMLVEDYDSLIEKMSNWNFQIFELVEKMGEKSGRILSQVM
                     YTLFQDTGLLETFKIPTQEFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIP
                     GLQQLHNNHETETKADSDARLSSGQIAYLSSKSCCIPDKSYGCLSSNIPALELMALYV
                     AAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNL
                     DHMEFKRFRFLVIEAILATDLKKHFEFLAEFNAKANDVNSNGIEWSSENDRLLVCQVC
                     IKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQES
                     FITHIVGPLCNSYDAAGLLPGQWIEAEEGDDTESDDDDDDDDDDDDDDDDDDEELDSD
                     DEETEDNLNPKPQRRKGRRRIFCQLMHHLTENHKIWKEIIEEEEKCKAEGNKLQVDNA
                     SLPQADEIQVIEEADEEEEQMFE"
     exon            1229..1279
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1528
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1668
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1775
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1986
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2060
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2200
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2338
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2450
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2564
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2764
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2968
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..3130
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3419
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..5368
                     /gene="Pde3b"
                     /gene_synonym="CGI PDE; RcGIPl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aactgttgca gcgcgcggga gctgggaggc gacgcagccg tctcccgtcc cgagccgtgc
       61 ctgaggggaa agggaaaagg cgtccgcggc agccggccct gggacaccga gaatcccctc
      121 tcgccgctct ccactgcggg catgaggcgg acgccgccgc ccctcagccc gcgcgccggg
      181 gatccggggc ggcggcggcg gcaggcgcag ccgtgaccgc tctgcgaagc tcggggcgcc
      241 cggaacgggg agggcgcggg gtcggcgagc gccagcggcc tcccggcagc ccggggctat
      301 gaggaaggac gagcgcgagc gggacacgcc agccatgagg tccccgccgc cgccgccgcc
      361 gccggccacg gccacggccg cctcgccccc cgagagcctg cgcaacggct acgtgaagag
      421 ctgcgtgagc ccgctgcggc aggaccctcc gcgcagcttc ttcttccacc tctgccgctt
      481 ctgcaacgtg gagccgccgg cggcctcgct ccgcgccggg gcacgcctct cgctcgccgc
      541 tctggccgcc tttgtcctgg ccgcgctgct gggcgcgggg cccgagcgct gggcggccgc
      601 ggcgaccggg cttcggacgc tgctgagcgc ttgctcgctc agcctcagcc cgctcttcag
      661 catcgcctgt gccttcttct tcctcacctg cttcctgacg cgcgcgcagc gcggcccgga
      721 ccgcggcgcc ggctcctggt ggctgctggc gctgcctgcc tgctgctacc tgggcgactt
      781 cgcggcgtgg cagtggtggt cgtggctgcg cggggagccg gcggcggcgg cggccggccg
      841 gctctgcctg gtgctgagct gcgtggggct gctgacgctc gcgcctcgcg tgaggctgcg
      901 gcacggagtc ctggtgctgc tcttcgccgg cctggtgtgg tgggtgtcct tctccggcct
      961 cggggctctg ccgcccgcgc tcaggcctct gctgtcgtgc ctggtcgggg gcgcgggatg
     1021 cctgctagcc ctgggcttgg accacttctt tcacgtccgg ggagcctcac ctcccccgcg
     1081 ctcggcgagt accgcggatg aaaaagtgcc tgtgatcaga ccccggagga ggtccagctg
     1141 cgtgtcgctg ggagaaagcg cggccggtta ctatggcagt ggcaagatgt tcaggagacc
     1201 gtcgttgcct tgtatttctc gagaacagat gattctctgg gactgggact tgaagcagtg
     1261 gtgtaaacct cattaccaga attctggagg tggaaatgga gttgatcttt cagtgctaaa
     1321 tgaagctcgc aatatggtgt cagaccttct gattgaccca agccttcccc cacaagtcat
     1381 ttcttctttg cggagtatca gtagcttgat gggtgctttc tcaggttcct gtaggccaaa
     1441 gattaattct ttcacaccat ttcctggatt ttatccctgt tctgaagtag aagatccagt
     1501 agagaaaggc gatcgaaaac ttcacaaggg gttgagtagc aaacccagtt tcccgactgc
     1561 acagctgagg aggagctcag gagcgtcagg attgctgacc agtgagcacc actcacggtg
     1621 ggatcgcagc ggtggcaaaa ggccttacca agaactcagt gtttcaagtc atggatgcca
     1681 cctaaatggg ccttttagtt caaatcttat gacaattcca aagcagaggt catcatctgt
     1741 gtcgctgacg caccatgcag gtctgagaag agctggtgct ttgcccagcc ccagtcttct
     1801 gaattcttca agccatgtac ccgtgtctgc tggctgtcta actaatcgat cacccgtagg
     1861 atttcttgat accagtgatt ttcttactaa gccaagtgtt actttacata gatcactggg
     1921 cagtgtatcc agtgcagcag atttccatca gtaccttagg aactctgaca gcagtctgtg
     1981 tagcagctgt ggacaccaaa tactcaagta tgtttcgaca tgtgaacctg atggtacaga
     2041 ccaccacaat gaaaaatcag gtgaagaaga cagcactgtt ttctcaaaag aacgattgaa
     2101 cattgtggaa actcaagaag aagagacagt gaaggaagac tgcagggaat tatttttgga
     2161 aggtgatgat cacctaatgg aagaggccca gcaaccgaat attgaccagg aagtgttatt
     2221 ggacccgatg ttagtggaag attatgattc attaatagaa aagatgagca actggaactt
     2281 tcagattttt gagcttgtag aaaaaatggg agaaaaatca ggaaggattc tcagccaggt
     2341 tatgtatact ttatttcaag atactggttt attggaaaca tttaaaattc ccactcaaga
     2401 atttatgaat tattttcgtg cactagaaaa tggctaccga gatattccat atcacaatcg
     2461 tgtgcatgcc acagatgtcc tacatgctgt ttggtatttg acaacacgac caattcctgg
     2521 cttacagcag ctccataata accatgaaac agaaaccaaa gcagattcag atgctagact
     2581 tagttctgga cagattgctt acctttcttc gaagagttgc tgtattccag ataagagtta
     2641 tggctgcctg tcttcaaaca ttcctgcgtt agaactgatg gctttatatg tggcagctgc
     2701 catgcacgat tatgatcacc caggaagaac aaatgcattc ctagtggcta caaatgcacc
     2761 tcaggcagtt ttatacaatg acagatctgt tctagaaaat catcatgccg catcagcgtg
     2821 gaatctgtat ctttctcgcc cagaatacaa cttcctcctt aaccttgatc acatggaatt
     2881 caagcgtttt cgatttttag tcatagaagc aatccttgct acagatctca aaaaacattt
     2941 tgagttcctt gctgaattca atgccaaggc caatgatgta aatagtaacg gtatagaatg
     3001 gagcagtgaa aacgatcgac tcttagtctg ccaggtgtgc atcaaattag cagacatcaa
     3061 cggcccagca aaagatcggg atcttcattt gagatggaca gaaggcattg tgaatgagtt
     3121 ttatgagcag ggagatgaag aagcaaccct gggtctacct attagtccct tcatggatcg
     3181 ttcttctcct caactagcaa aactccaaga gtcatttatc acccacattg tgggccccct
     3241 gtgcaactcc tatgatgctg ctggcttgct gcctggtcag tggatagaag cagaagaggg
     3301 tgacgataca gaaagtgatg atgatgatga tgatgatgat gatgatgatg atgatgatga
     3361 tgatgatgat gaagagttag attcagatga tgaagaaaca gaagacaatc taaatcctaa
     3421 accacaaaga aggaaaggca ggcggcgaat attttgccaa ctaatgcacc acctcactga
     3481 aaaccacaag atctggaagg aaatcataga agaggaagaa aaatgtaaag ctgaggggaa
     3541 caaactgcag gtggataatg cctccttacc tcaggcagat gagattcagg tcattgaaga
     3601 agcagatgaa gaggaagaac aaatgtttga atgagaagaa aactcatgct gaagaagcct
     3661 ggggtgctct tcccagggtc gttacctagt gctcaccgta ctgattctca actgaccatt
     3721 cccatgtgga caggccttaa tactgtgagg ggatccttgc taccttggta gttctcactc
     3781 ctaagcactt tgattacaga ctagagactg accttagagc tttctgcagt tgagtgatgc
     3841 tgcagagcct tctccaagcc ttcactggtg tgtaaatagt gtgtcataat gctgctttgc
     3901 tgggtagtga gctctcgttt gtcatggtga gggagggagg tagggaggga gggagggatg
     3961 gagggatgga gggaaggagg gagggctaac acagaaaagt caagtgacct tggttttcaa
     4021 agtgaccata attcttttat gaaaacagga tacctcgggt atttccaaat atatcttcta
     4081 tgtctttgtt tgggggtggg atggggcaag aaacaaactt ctatgcttaa tccaaaaagg
     4141 aatttatcac ggcctttctg agtgatttct gctcgtggca gtacactgtt ccatgtggtt
     4201 ctcctagctt catccgtgaa ggactgagga cctttgttat acttaacaaa acccagatgc
     4261 atcaatttct gatgcttttt actgttgtgt ataatctact taagtgtttt atttctgccg
     4321 aaagtattca ggtttgctgt ggacatcagg agtctgaatt ctgttcttac tgattttgtt
     4381 ccatggttga attttaaaag tgtttaacaa tgaaggaact ttattcttta gtcaaaactg
     4441 ttatttttat tcttgctcag gatacataat tttaaacatt aattattata aacgtggcat
     4501 agatttgcca gaaaaaaaac attgtcctaa tattgatgtt accttttatg aaatgagcac
     4561 cattttcttt taattaaagc caagaaaaag gagatgacta tcttttgcct tagtggaaaa
     4621 tcaaatgaaa acaaaaccca ttgaaggtat tgcctaaata attagttatc tttaaatatg
     4681 tgtcttaggt gtcttgtatt cttgtgtctg gttatagtaa aactgacaat ttttgttact
     4741 acctttctgc ttcaagggga caatggcaga gatgacacat cactatgtag taatactaca
     4801 gccccacaga aagactgaat tttgaagtaa tgtatatgat gggaattcat attaattttt
     4861 acattaaaat gctatgaata gttcaataaa caattccata aagtacatat aaataattta
     4921 ctaagaaaaa gacaacattt atgtttacat aacctcatta gagtacagca attgtggaaa
     4981 attactgaag ttatacaaag caaccaaaat gataccaaga tggttaactg atttctcaag
     5041 tgatacttta agaccataca atattttgga gccattgaac tttgtgcaca gttaactgtt
     5101 agtttctaca gtggttccta tatagtatga tgctagaatt tcttgcctgc ctagttattt
     5161 aggttgtcta taagaccgca gttaaagttg gattggaata ctggtatttt tgaccctgca
     5221 ggtaccatat ttaatgaatc actacacttt atttattaaa catttgaggg ttgagttcct
     5281 tcatgtcact tttgctacca caaccacatg atttgtaaac tggttccagc atctattact
     5341 aagtaataaa attttaactg ataatcta
//
