LOCUS       NM_031079               4013 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus phosphodiesterase 2A (Pde2a), transcript variant
            2, mRNA.
ACCESSION   NM_031079
VERSION     NM_031079.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Skryabin,E.B., De Jong,K.A., Subramanian,H., Bork,N.I., Froese,A.,
            Skryabin,B.V. and Nikolaev,V.O.
  TITLE     CRISPR/Cas9 Knock-Out in Primary Neonatal and Adult Cardiomyocytes
            Reveals Distinct cAMP Dynamics Regulation by Various PDE2A and
            PDE3A Isoforms
  JOURNAL   Cells 12 (11), 1543 (2023)
   PUBMED   37296663
  REMARK    GeneRIF: CRISPR/Cas9 Knock-Out in Primary Neonatal and Adult
            Cardiomyocytes Reveals Distinct cAMP Dynamics Regulation by Various
            PDE2A and PDE3A Isoforms.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Liu,D., Wang,Z., Nicolas,V., Lindner,M., Mika,D., Vandecasteele,G.,
            Fischmeister,R. and Brenner,C.
  TITLE     PDE2 regulates membrane potential, respiration and permeability
            transition of rodent subsarcolemmal cardiac mitochondria
  JOURNAL   Mitochondrion 47, 64-75 (2019)
   PUBMED   31100470
  REMARK    GeneRIF: PDE2 regulates membrane potential, respiration and
            permeability transition of rodent subsarcolemmal cardiac
            mitochondria.
REFERENCE   3  (bases 1 to 4013)
  AUTHORS   Monterisi,S., Lobo,M.J., Livie,C., Castle,J.C., Weinberger,M.,
            Baillie,G., Surdo,N.C., Musheshe,N., Stangherlin,A., Gottlieb,E.,
            Maizels,R., Bortolozzi,M., Micaroni,M. and Zaccolo,M.
  TITLE     PDE2A2 regulates mitochondria morphology and apoptotic cell death
            via local modulation of cAMP/PKA signalling
  JOURNAL   Elife 6, e21374 (2017)
   PUBMED   28463107
  REMARK    GeneRIF: Using pharmacological and genetic approaches combined with
            real-time FRET imaging and high resolution microscopy, we
            demonstrate that in rat cardiac myocytes and other cell types
            mitochondrial PDE2A2 regulates local cAMP levels and PKA-dependent
            phosphorylation of Drp1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4013)
  AUTHORS   Zoccarato,A., Surdo,N.C., Aronsen,J.M., Fields,L.A., Mancuso,L.,
            Dodoni,G., Stangherlin,A., Livie,C., Jiang,H., Sin,Y.Y.,
            Gesellchen,F., Terrin,A., Baillie,G.S., Nicklin,S.A., Graham,D.,
            Szabo-Fresnais,N., Krall,J., Vandeput,F., Movsesian,M., Furlan,L.,
            Corsetti,V., Hamilton,G., Lefkimmiatis,K., Sjaastad,I. and
            Zaccolo,M.
  TITLE     Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated
            by Phosphodiesterase 2
  JOURNAL   Circ Res 117 (8), 707-719 (2015)
   PUBMED   26243800
  REMARK    GeneRIF: Cardiac hypertrophy is inhibited by a local pool of cAMP
            regulated by PDE2A.
REFERENCE   5  (bases 1 to 4013)
  AUTHORS   Li,D., Lu,C.J., Hao,G., Wright,H., Woodward,L., Liu,K., Vergari,E.,
            Surdo,N.C., Herring,N., Zaccolo,M. and Paterson,D.J.
  TITLE     Efficacy of B-Type Natriuretic Peptide Is Coupled to
            Phosphodiesterase 2A in Cardiac Sympathetic Neurons
  JOURNAL   Hypertension 66 (1), 190-198 (2015)
   PUBMED   25916722
  REMARK    GeneRIF: Pde2 overexpression decreased BNP stimulation of cGMP and
            BNP responses to the Ca current, intracellular Ca transient, and
            neurotransmitter release. Pde2 was higher in stellate neurons from
            prohypertensive rats vs normotensives.
REFERENCE   6  (bases 1 to 4013)
  AUTHORS   Gustafsson,A.B. and Brunton,L.L.
  TITLE     Attenuation of cAMP accumulation in adult rat cardiac fibroblasts
            by IL-1beta and NO: role of cGMP-stimulated PDE2
  JOURNAL   Am J Physiol Cell Physiol 283 (2), C463-C471 (2002)
   PUBMED   12107056
REFERENCE   7  (bases 1 to 4013)
  AUTHORS   Sadhu,K., Hensley,K., Florio,V.A. and Wolda,S.L.
  TITLE     Differential expression of the cyclic GMP-stimulated
            phosphodiesterase PDE2A in human venous and capillary endothelial
            cells
  JOURNAL   J Histochem Cytochem 47 (7), 895-906 (1999)
   PUBMED   10375378
REFERENCE   8  (bases 1 to 4013)
  AUTHORS   Cherry,J.A. and Davis,R.L.
  TITLE     A mouse homolog of dunce, a gene important for learning and memory
            in Drosophila, is preferentially expressed in olfactory receptor
            neurons
  JOURNAL   J Neurobiol 28 (1), 102-113 (1995)
   PUBMED   8586960
REFERENCE   9  (bases 1 to 4013)
  AUTHORS   Yang,Q., Paskind,M., Bolger,G., Thompson,W.J., Repaske,D.R.,
            Cutler,L.S. and Epstein,P.M.
  TITLE     A novel cyclic GMP stimulated phosphodiesterase from rat brain
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1850-1858 (1994)
   PUBMED   7811274
REFERENCE   10 (bases 1 to 4013)
  AUTHORS   Repaske,D.R., Swinnen,J.V., Jin,S.L., Van Wyk,J.J. and Conti,M.
  TITLE     A polymerase chain reaction strategy to identify and clone cyclic
            nucleotide phosphodiesterase cDNAs. Molecular cloning of the cDNA
            encoding the 63-kDa calmodulin-dependent phosphodiesterase
  JOURNAL   J Biol Chem 267 (26), 18683-18688 (1992)
   PUBMED   1326532
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Jan 18, 2018 this sequence version replaced NM_031079.1.
            
            Transcript Variant: This variant (2) represents use of an alternate
            promoter and has a distinct 5' UTR and 5' coding region, compared
            to variant 1. The resulting isoform (2) has a shorter and distinct
            N-terminus, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U21101.1, SRR26360194.916523.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               JAXUCZ010000001.1  165266009-165266194
            187-276             JAXUCZ010000001.1  165296756-165296845
            277-365             JAXUCZ010000001.1  165299855-165299943
            366-475             JAXUCZ010000001.1  165310119-165310228
            476-531             JAXUCZ010000001.1  165311088-165311143
            532-591             JAXUCZ010000001.1  165315842-165315901
            592-733             JAXUCZ010000001.1  165316403-165316544
            734-786             JAXUCZ010000001.1  165316792-165316844
            787-861             JAXUCZ010000001.1  165317031-165317105
            862-903             JAXUCZ010000001.1  165317191-165317232
            904-972             JAXUCZ010000001.1  165317706-165317774
            973-1103            JAXUCZ010000001.1  165317963-165318093
            1104-1215           JAXUCZ010000001.1  165318853-165318964
            1216-1255           JAXUCZ010000001.1  165319382-165319421
            1256-1319           JAXUCZ010000001.1  165319526-165319589
            1320-1392           JAXUCZ010000001.1  165319932-165320004
            1393-1570           JAXUCZ010000001.1  165320126-165320303
            1571-1683           JAXUCZ010000001.1  165320531-165320643
            1684-1761           JAXUCZ010000001.1  165321407-165321484
            1762-1884           JAXUCZ010000001.1  165321819-165321941
            1885-1955           JAXUCZ010000001.1  165322484-165322554
            1956-2078           JAXUCZ010000001.1  165322815-165322937
            2079-2166           JAXUCZ010000001.1  165323246-165323333
            2167-2214           JAXUCZ010000001.1  165323511-165323558
            2215-2289           JAXUCZ010000001.1  165324516-165324590
            2290-2389           JAXUCZ010000001.1  165324724-165324823
            2390-2502           JAXUCZ010000001.1  165325085-165325197
            2503-2541           JAXUCZ010000001.1  165325564-165325602
            2542-2648           JAXUCZ010000001.1  165325758-165325864
            2649-4013           JAXUCZ010000001.1  165326102-165327466
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..4013
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /note="phosphodiesterase 2A"
                     /db_xref="GeneID:81743"
                     /db_xref="RGD:620965"
     exon            1..186
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2856
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /EC_number="3.1.4.17"
                     /note="isoform PDE2A2 is encoded by transcript variant 2;
                     cGMP-dependent 3',5'-cyclic phosphodiesterase; cyclic
                     GMP-stimulated phosphodiesterase; phosphodiesterase 2A,
                     cGMP-stimulated"
                     /codon_start=1
                     /product="cGMP-dependent 3',5'-cyclic phosphodiesterase
                     isoform PDE2A2"
                     /protein_id="NP_112341.1"
                     /db_xref="GeneID:81743"
                     /db_xref="RGD:620965"
                     /translation="MVLVLHHILIAVVQFLRRGQQVFLKPDEPPPQPCADSLQDALLS
                     LGAVIDIAGLRQAAKDALSAVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAV
                     ISRKRLSCDGLGPSDLLGKPLARLVAPLAPDTQVLVIPLLDKETGTVAAVILVHCGQL
                     SDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSEDQKDEKGYTAHDRKIL
                     QLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGEEVS
                     FPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVAL
                     ACAFNKLGGDFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLF
                     THLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPAD
                     QGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAEL
                     VNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSHLANEMMMYHMKVS
                     DDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDC
                     PTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCM
                     CHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRK
                     DYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLS
                     DQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAM
                     PIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLDEEYEVPDLD
                     VTRAPVNGCCSLEG"
     exon            187..276
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            277..365
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            366..475
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            476..531
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            532..591
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            592..733
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            734..786
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            787..861
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            862..903
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            904..972
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1103
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1215
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1255
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1319
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1392
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1570
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1683
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1761
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1884
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1955
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2078
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2166
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2214
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2289
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2389
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2502
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2541
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2648
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2649..4013
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; Pde2; Pde2a2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaagctgttg cggacacgct gaccgagcgc agcgggccgg gctggagggg cgggcgggcg
       61 gctacgagca tggtcctggt gttgcaccac atcctcatcg ctgttgtcca attcctcagg
      121 cggggccagc aggtcttcct caagccggac gaaccgccgc cgcagccatg cgccgacagc
      181 ctgcaggatg ctttgctgag cctgggcgcc gttatcgaca tcgcgggcct tcgacaggct
      241 gccaaggatg ccctctcggc cgtgctcccc aaagtggaga ctgtctacac ctacctggta
      301 gatggggagt ccaggctggt gtgtgaggac ccccctcatg agctgccaca ggaaggaaaa
      361 attcgagagg ctgtgatttc tcggaagcgg ctgagctgcg atgggctggg accttcagac
      421 ctactaggaa agcccttggc caggctggtg gctccactgg cccctgacac gcaagtgctg
      481 gtcatacccc tgctggacaa ggagactgga actgtggcag ccgtcatctt ggtgcactgt
      541 ggccagctga gtgacagtga ggagcagagc ctgcaggtgg tggagaagca tgctctggtg
      601 gccctgcaga gggtgcaggc cctgcagcag cgcaggcctg aagctgttca gaacacctca
      661 gcggatccct ctgaagatca aaaggatgag aagggataca ccgcccatga ccgaaagatc
      721 ctgcaactgt gtggagaact ctatgacttg gatgccactt ctctgcagct caaagtcctt
      781 cgatatcttc agcaggagac acaggccact cactgctgcc tcctgctggt gtcagaggac
      841 aacctgcagc tttcctgcaa ggtcattgga gagaaagtgc tgggagaaga ggtcagcttt
      901 ccattgacca tgggacgcct gggccaggtg gtggaggaca aacagtgtat ccagttgaag
      961 gacctaacct ctgacgatgt gcaacagcta caaaacatgt tgggctgtga gcttcgggct
     1021 atgctatgtg tccctgtcat cagtcgagcc actgaccagg tggtggccct ggcttgcgcc
     1081 ttcaacaagc ttggaggaga cttcttcaca gatgaggatg aacgtgcgat ccaacactgc
     1141 ttccactaca caggcacggt gctcaccagt accttggcct tccagaagga gcagaagctc
     1201 aagtgtgagt gccaggctct tctccaagtg gcaaagaacc tcttcaccca cctggatgac
     1261 gtctctgtcc tgctacagga gatcatcaca gaggccagaa acctcagcaa cgcagagatc
     1321 tgctccgtgt tcctgctgga tcagaatgag ctggttgcca aggtgttcga tggcggagta
     1381 gtggacgatg agagttatga gatccgcatc cctgcggacc aaggcatcgc gggccacgtg
     1441 gcgaccacgg gccagatcct gaacatccca gatgcatacg cccatccgct tttctatcgc
     1501 ggcgtagacg acagcactgg cttccgcacg cgcaacattc tctgcttccc tatcaagaac
     1561 gagaaccaag aggtcatcgg tgtggctgag ctagtgaaca agatcaatgg gccatggttc
     1621 agcaaatttg atgaggacct ggccacagcc ttctccatct actgtggcat tagcatcgct
     1681 cactctctcc tatacaaaaa ggtgaatgaa gcccagtacc gcagccacct ggccaacgag
     1741 atgatgatgt accatatgaa ggtctctgac gatgaataca ccaaacttct ccatgatggc
     1801 atccaacctg tggccgccat tgactccaac tttgccaact tcacctatac tcctcggtct
     1861 ctgcctgagg acgatacttc tatggccatc ctgagcatgc tgcaagacat gaacttcatc
     1921 aataactaca aaattgactg cccaactctg gcccggttct gcctgatggt gaagaaaggc
     1981 taccgggatc cgccctacca caactggatg cacgccttct ctgtctctca cttttgctac
     2041 ttgctctaca agaatctgga gctctccaac tacctcgagg acatcgagat ctttgcgttg
     2101 tttatttcct gcatgtgtca cgacctggac cacagaggca caaacaactc cttccaggtg
     2161 gcctcgaaat ctgtgctggc tgcactctac agctcagagg gctctgtcat ggagaggcac
     2221 cactttgctc aggccattgc tatcctcaac acccatggct gtaatatctt tgaccacttc
     2281 tctcggaagg actatcagcg aatgctggac ctgatgaggg acatcatctt ggccacggac
     2341 ctggcacacc acctccgcat cttcaaggac ctgcagaaga tggctgaagt gggttatgac
     2401 cgaaacaaca agcaacacca caggcttctt ctatgcctcc ttatgacctc ctgtgacctc
     2461 tctgaccaga caaagggctg gaagaccacc agaaagattg cggagctgat ctacaaagag
     2521 ttcttctccc aaggagactt ggagaaggcc atgggcaacc gaccaatgga gatgatggat
     2581 cgggagaagg cctacatccc tgaacttcag atcagcttta tggagcacat cgccatgccc
     2641 atctacaagc tgttacaaga cctgttcccc aaggcagcag agctgtatga acgcgtggcc
     2701 tccaatcgtg agcactggac caaggtgtcc cacaagttca ccatccgagg cctccccagt
     2761 aacaactcgc tggatttcct ggatgaggaa tatgaggtcc ctgatttgga cgtcaccagg
     2821 gctcctgtca atggctgctg cagcctcgag ggctgagtga tcccctctag ggaccctccc
     2881 tgcccggccc tcctttcaca actctctacc agtctgtctg atgcattggg aaccagagcc
     2941 atgggtcctg ggttctagac caggatgtcc cctgtgacct ggcacatgcc acccttcctg
     3001 ggcttcgtct ttctcatctg cagaaggaat tcgagattcg cagtctcaca cagacttggc
     3061 cgtttgttct tcaagagcac agtagtgacc agtggcagtg ggcttccttc tgcacctctg
     3121 actgtaccgt gcccagttct gcccaagcca ctccatcttt gagccacaga accttctgtg
     3181 aacccattag agccccatta cgtcagtatc cttgttttcc tggaggaatc ccaggtctct
     3241 ggagacttcg agatcctcat ggaagaagga gaggccaccg agagagcaga gggtataaat
     3301 cagtcaccag ctagaagtcg ccatagggtg aagggaccct gagggtgcct ctgtgcttct
     3361 ggcaggactg ggaccctgga ggaagctgac agccaggttg ctcagtgctc tgcaggaggc
     3421 agtgctccag cagagaaggc aggaataagg gacagaagga tcctcaagct gggagacagt
     3481 ccctacacac ccctgccctt caggtgttgt gacctgaagt gagttccttt ccctagctag
     3541 ccaagacagg atcattagac agcctattct ctctttagat caaagtctcc gtcattctgt
     3601 ggagtccgtg agtggttgtg gcatgctctg ccaccctgag gcacagagag cctaacctcc
     3661 tttcctctga gtgtatcccc ctggtctgag cccctctccc attcctgcca tcagagaggg
     3721 gcctggcctc tctccacagg gcttctctcc tcttcaaggc catatccaca cctgcccccc
     3781 ggagggctca ggagactctc ccagggctgg gcctcttggg tgagtggcca ctagcttctc
     3841 cttctgtttt gttctgtatg tattgtgggt gggggagggg gccacctgcc ttacctactc
     3901 tgagttgcct ttagagagat gcatttttct aggactctgt gcacctgttg ttgtatgtgg
     3961 tcccgtgggc tgagtgcttt gtacatgaga ataaatctat ttctttctac caa
//
