LOCUS       NM_030871               2081 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus phosphodiesterase 1A (Pde1a), mRNA.
ACCESSION   NM_030871 XM_579497
VERSION     NM_030871.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Khammy,M.M., Dalsgaard,T., Larsen,P.H., Christoffersen,C.T.,
            Clausen,D., Rasmussen,L.K., Folkersen,L., Grunnet,M., Kehler,J.,
            Aalkjaer,C. and Nielsen,J.
  TITLE     PDE1A inhibition elicits cGMP-dependent relaxation of rat
            mesenteric arteries
  JOURNAL   Br J Pharmacol 174 (22), 4186-4198 (2017)
   PUBMED   28910498
  REMARK    GeneRIF: Pde1a is the dominant PDE1 isoform present in vascular
            smooth muscle cells, and relaxation mediated by PDE1A inhibition is
            predominantly driven by enhanced cGMP signalling.
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Miller,C.L., Cai,Y., Oikawa,M., Thomas,T., Dostmann,W.R.,
            Zaccolo,M., Fujiwara,K. and Yan,C.
  TITLE     Cyclic nucleotide phosphodiesterase 1A: a key regulator of cardiac
            fibroblast activation and extracellular matrix remodeling in the
            heart
  JOURNAL   Basic Res Cardiol 106 (6), 1023-1039 (2011)
   PUBMED   22012077
  REMARK    GeneRIF: These results suggest that induction of PDE1A plays a
            critical role in cardiac fibroblast activation and cardiac fibrosis
REFERENCE   3  (bases 1 to 2081)
  AUTHORS   Giachini,F.R., Lima,V.V., Carneiro,F.S., Tostes,R.C. and Webb,R.C.
  TITLE     Decreased cGMP level contributes to increased contraction in
            arteries from hypertensive rats: role of phosphodiesterase 1
  JOURNAL   Hypertension 57 (3), 655-663 (2011)
   PUBMED   21282562
  REMARK    GeneRIF: We conclude that PDE1 activation reduces cGMP
            bioavailability in arteries from Ang II, contributing to increased
            contractile responsiveness.
REFERENCE   4  (bases 1 to 2081)
  AUTHORS   Jeon,K.I., Jono,H., Miller,C.L., Cai,Y., Lim,S., Liu,X., Gao,P.,
            Abe,J., Li,J.D. and Yan,C.
  TITLE     Ca2+/calmodulin-stimulated PDE1 regulates the beta-catenin/TCF
            signaling through PP2A B56 gamma subunit in proliferating vascular
            smooth muscle cells
  JOURNAL   FEBS J 277 (24), 5026-5039 (2010)
   PUBMED   21078118
  REMARK    GeneRIF: findings provide direct evidence for the first time that
            PP2A B56gamma is a critical mediator for PDE1A in the regulation of
            beta-catenin signaling in proliferating VSMCs
REFERENCE   5  (bases 1 to 2081)
  AUTHORS   Wang,J., Bingaman,S. and Huxley,V.H.
  TITLE     Intrinsic sex-specific differences in microvascular endothelial
            cell phosphodiesterases
  JOURNAL   Am J Physiol Heart Circ Physiol 298 (4), H1146-H1154 (2010)
   PUBMED   20139324
  REMARK    GeneRIF: Expression mRNA levels of PDE1A (male > female) and PDE3B
            (male < female) were sex dependent in microvascular endothelial
            cells.
REFERENCE   6  (bases 1 to 2081)
  AUTHORS   Santone,R., Giorgi,M., Maccarone,R., Basso,M., Deplano,S. and
            Bisti,S.
  TITLE     Gene expression and protein localization of calmodulin-dependent
            phosphodiesterase in adult rat retina
  JOURNAL   J Neurosci Res 84 (5), 1020-1026 (2006)
   PUBMED   16881052
REFERENCE   7  (bases 1 to 2081)
  AUTHORS   Nagel,D.J., Aizawa,T., Jeon,K.I., Liu,W., Mohan,A., Wei,H.,
            Miano,J.M., Florio,V.A., Gao,P., Korshunov,V.A., Berk,B.C. and
            Yan,C.
  TITLE     Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in
            vascular smooth muscle cell growth and survival
  JOURNAL   Circ Res 98 (6), 777-784 (2006)
   PUBMED   16514069
  REMARK    GeneRIF: PDE1A is important in VSMC growth and survival and may
            contribute to the neointima formation in atherosclerosis and
            restenosis.
REFERENCE   8  (bases 1 to 2081)
  AUTHORS   Vasta,V., Sonnenburg,W.K., Yan,C., Soderling,S.H.,
            Shimizu-Albergine,M. and Beavo,J.A.
  TITLE     Identification of a new variant of PDE1A calmodulin-stimulated
            cyclic nucleotide phosphodiesterase expressed in mouse sperm
  JOURNAL   Biol Reprod 73 (4), 598-609 (2005)
   PUBMED   15901640
REFERENCE   9  (bases 1 to 2081)
  AUTHORS   Yanaka,N., Kurosawa,Y., Minami,K., Kawai,E. and Omori,K.
  TITLE     cGMP-phosphodiesterase activity is up-regulated in response to
            pressure overload of rat ventricles
  JOURNAL   Biosci Biotechnol Biochem 67 (5), 973-979 (2003)
   PUBMED   12834273
REFERENCE   10 (bases 1 to 2081)
  AUTHORS   Cho,C.H., Cho,D.H., Seo,M.R. and Juhnn,Y.S.
  TITLE     Differential changes in the expression of cyclic nucleotide
            phosphodiesterase isoforms in rat brains by chronic treatment with
            electroconvulsive shock
  JOURNAL   Exp Mol Med 32 (3), 110-114 (2000)
   PUBMED   11048640
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF327838.1.
            
            [WARNING] On Jul 16, 2024 this sequence was replaced by
            NM_030871.2.
            
            On Apr 19, 2005 this sequence version replaced XM_579497.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF327838.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00155305,
                                           SAMEA5756307 [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q24"
     gene            1..2081
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /note="phosphodiesterase 1A"
                     /db_xref="GeneID:81529"
                     /db_xref="RGD:68329"
     exon            1..66
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            67..177
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1705
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /EC_number="3.1.4.17"
                     /note="3'-RACE clone 8 phosphodiesterase 1A; 3-RACE clone
                     8 phosphodiesterase 1A; calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A;
                     phosphodiesterase 1A, calmodulin-dependent"
                     /codon_start=1
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A"
                     /protein_id="NP_110498.1"
                     /db_xref="GeneID:81529"
                     /db_xref="RGD:68329"
                     /translation="MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEK
                     GDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFT
                     RKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKNYHMVGLTYPAAVIVTLKEVDKW
                     SFDVFALNEASGEHSLKFMIYELFTSNDLINRFKIPVSCLIAFAEALEVGYSKHKNPY
                     HNLVHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAVHDYEHTGTTNNFHIQTRS
                     DVAILYNDRSVLENHHVSAAYRLMQEEEMNILVNLSKDDWRDLRNLVIETVLATDMSG
                     HFQQIKNIRSSLQQPEGIDRAKTMSLILHAADISHPAKTWKLHYRWTMALMEEFFLQG
                     DKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKP
                     ESSNYGASSSSTMIGFHVADALRRSNTKGCMSDGTYAPDYSLSAVDLKSFKNNLVDII
                     QQNKERWKELAAQGELDLHKNSEDLEEKHADTHP"
     exon            178..291
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            292..474
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            475..541
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            542..658
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            659..799
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            800..900
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1026
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1128
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1249
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1331
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1452
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1640
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1748
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..2081
                     /gene="Pde1a"
                     /gene_synonym="3'-RACEclone8phosphodiesterase1A;
                     3-RACEclone8phosphodiesterase1A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaggatagcc tgaacagtcg gtcagtagta cagtagcagc tcacatgtgt ggattgctcc
       61 cgtgaggaac atcattatgg gctctacaga tacagacatc gaagaactgg aaaacgccac
      121 ttacaagtat ctcattggag aacagaccga gaagatgtgg caacgcctga aaggaatact
      181 aagatgctta gtgaagcagc tggagaaagg tgatgtcaac gttgtcgacc taaagaagaa
      241 tatcgaatat gcggcatctg tactggaagc tgtttacatc gacgagacga ggaggcttct
      301 ggatactgag gatgagctca gcgacattca gaccgactcg gttccttcgg aagtccggga
      361 ctggttggct tcaaccttta cacggaaaat ggggatgacg aaaaagaaac ccgaggaaaa
      421 accgaagttt cgcagcattg tccacgctgt ccaggctggg atttttgtgg aaaggatgta
      481 caggaagaac tatcacatgg ttggtttgac atatcctgca gctgtcatag tgacactcaa
      541 ggaagttgat aaatggtctt ttgatgtatt tgccttaaat gaagcaagcg gggagcatag
      601 tctgaagttt atgatttatg aactatttac cagtaatgat cttatcaacc gcttcaagat
      661 tcctgtttct tgcctaattg cctttgcaga agctttagaa gttggttaca gcaagcacaa
      721 aaatccgtat cataatttgg tccatgcagc tgatgtcact caaactgtgc attacataat
      781 gcttcataca ggtatcatgc actggctcac tgaactggaa atcttagcca tggtctttgc
      841 agccgccgtt catgactatg aacacacagg aactacaaac aacttccata ttcagacgag
      901 gtcagacgtc gccattctgt ataacgaccg ctcagttctt gagaatcacc atgtcagcgc
      961 agcctacaga cttatgcaag aggaggaaat gaatattttg gtaaatttat ctaaagatga
     1021 ctggagggat cttcggaact tagtgattga aacggtctta gcaacagaca tgtcaggcca
     1081 tttccagcaa attaaaaaca taagaagcag cttgcagcag cctgaaggga ttgacagagc
     1141 caaaacgatg tccctgatac tccacgcagc cgacatcagc cacccagcca aaacgtggaa
     1201 gctgcactac aggtggacta tggccttaat ggaggaattt ttcctgcagg gagacaaaga
     1261 agctgaatta gggcttccgt tttctccact ttgtgatcgg aagtcaacga tggttgccca
     1321 gtctcaaata ggtttcattg atttcatagt ggagccaacg ttttctctcc tgacagactc
     1381 gacagagaaa attgttattc ctcttataga ggaagcttca aaaccagagt cttctaacta
     1441 tggggcaagc agctcgtcca ccatgattgg gttccatgtt gctgacgctc tgaggcggtc
     1501 aaacacaaaa ggctgcatga gtgatgggac ctatgcccca gactactctc tgtcagctgt
     1561 ggacctgaag agcttcaaaa acaacctggt ggacatcatt cagcagaaca aggagaggtg
     1621 gaaagagttg gctgcccaag gcgaacttga tcttcataag aactcagaag acttagaaga
     1681 aaagcatgct gacacacatc cataggtgtg aacgaacttt aagagttcag ttattttaaa
     1741 cacaagagga aaatgaaagc agcatgaaaa acatgcaaag accttgattt ttctgcaaaa
     1801 aaatatatgt ttggctcagg ccattttagt cgactcctcc ataaggaaca agagcataag
     1861 tttcactctt cttagctcag caaagcagta cctgcaagtt atcattagga ttctggctgc
     1921 tgtttctgcc atcgttctcc ctccatgatt tggccatctt caatgaagcc agagaaatct
     1981 ggagactttc aactttccgt catgtttgac ttggcctgaa gacagaatgg ttttcaatgc
     2041 acttcttgca ccgtgtcact caagaggacc cacaaccaca c
//
