LOCUS       NM_078622               1567 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Rattus norvegicus phosphate cytidylyltransferase 1A, choline
            (Pcyt1a), mRNA.
ACCESSION   NM_078622
VERSION     NM_078622.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Favale,N.O., Pescio,L.G., Santacreu,B.J., Marquez,M.G. and
            Sterin-Speziale,N.B.
  TITLE     Participation of prostaglandin D2 in the mobilization of the
            nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in
            renal epithelial cells
  JOURNAL   Biochim Biophys Acta 1861 (6), 513-523 (2016)
   PUBMED   27032756
  REMARK    GeneRIF: Data show that exogenous prostaglandin D2 (PGD2) induces
            CTP:phosphocholine cytidylyltransferase alpha (CCTalpha)
            distribution to nuclear envelope from intranuclear Lamin A/C foci.
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Lee,J., Taneva,S.G., Holland,B.W., Tieleman,D.P. and Cornell,R.B.
  TITLE     Structural basis for autoinhibition of CTP:phosphocholine
            cytidylyltransferase (CCT), the regulatory enzyme in
            phosphatidylcholine synthesis, by its membrane-binding amphipathic
            helix
  JOURNAL   J Biol Chem 289 (3), 1742-1755 (2014)
   PUBMED   24275660
  REMARK    GeneRIF: This work deciphers how the CCT regulatory amphipathic
            helix functions as a silencing device.
REFERENCE   3  (bases 1 to 1567)
  AUTHORS   Arsenault,D.J., Yoo,B.H., Rosen,K.V. and Ridgway,N.D.
  TITLE     ras-Induced up-regulation of CTP:phosphocholine
            cytidylyltransferase alpha contributes to malignant transformation
            of intestinal epithelial cells
  JOURNAL   J Biol Chem 288 (1), 633-643 (2013)
   PUBMED   23155050
  REMARK    GeneRIF: ras-Induced up-regulation of CTP:phosphocholine
            cytidylyltransferase alpha contributes to malignant transformation
            of intestinal epithelial cells
REFERENCE   4  (bases 1 to 1567)
  AUTHORS   Ding,Z., Taneva,S.G., Huang,H.K., Campbell,S.A., Semenec,L.,
            Chen,N. and Cornell,R.B.
  TITLE     A 22-mer segment in the structurally pliable regulatory domain of
            metazoan CTP: phosphocholine cytidylyltransferase facilitates both
            silencing and activating functions
  JOURNAL   J Biol Chem 287 (46), 38980-38991 (2012)
   PUBMED   22988242
  REMARK    GeneRIF: the 22-mer segment in the structurally pliable regulatory
            domain of rat CTP: phosphocholine cytidylyltransferase facilitates
            both silencing and activating functions
REFERENCE   5  (bases 1 to 1567)
  AUTHORS   Gehrig,K. and Ridgway,N.D.
  TITLE     CTP:phosphocholine cytidylyltransferase alpha (CCTalpha) and lamins
            alter nuclear membrane structure without affecting
            phosphatidylcholine synthesis
  JOURNAL   Biochim Biophys Acta 1811 (6), 377-385 (2011)
   PUBMED   21504799
  REMARK    GeneRIF: 127 fibroblasts was heterogeneous, with evidence of
            nucleoplasmic, cytoplasmic and NE staining.
REFERENCE   6  (bases 1 to 1567)
  AUTHORS   Cornell,R.B., Kalmar,G.B., Kay,R.J., Johnson,M.A., Sanghera,J.S.
            and Pelech,S.L.
  TITLE     Functions of the C-terminal domain of CTP: phosphocholine
            cytidylyltransferase. Effects of C-terminal deletions on enzyme
            activity, intracellular localization and phosphorylation potential
  JOURNAL   Biochem J 310 (Pt 2) (Pt 2), 699-708 (1995)
   PUBMED   7654214
REFERENCE   7  (bases 1 to 1567)
  AUTHORS   Sweitzer,T.D. and Kent,C.
  TITLE     Expression of wild-type and mutant rat liver CTP: phosphocholine
            cytidylyltransferase in a cytidylyltransferase-deficient Chinese
            hamster ovary cell line
  JOURNAL   Arch Biochem Biophys 311 (1), 107-116 (1994)
   PUBMED   8185307
REFERENCE   8  (bases 1 to 1567)
  AUTHORS   MacDonald,J.I. and Kent,C.
  TITLE     Identification of phosphorylation sites in rat liver CTP:
            phosphocholine cytidylyltransferase
  JOURNAL   J Biol Chem 269 (14), 10529-10537 (1994)
   PUBMED   8144639
REFERENCE   9  (bases 1 to 1567)
  AUTHORS   MacDonald,J.I. and Kent,C.
  TITLE     Baculovirus-mediated expression of rat liver CTP:phosphocholine
            cytidylyltransferase
  JOURNAL   Protein Expr Purif 4 (1), 1-7 (1993)
   PUBMED   8381041
REFERENCE   10 (bases 1 to 1567)
  AUTHORS   Kalmar,G.B., Kay,R.J., Lachance,A., Aebersold,R. and Cornell,R.B.
  TITLE     Cloning and expression of rat liver CTP: phosphocholine
            cytidylyltransferase: an amphipathic protein that controls
            phosphatidylcholine synthesis
  JOURNAL   Proc Natl Acad Sci U S A 87 (16), 6029-6033 (1990)
   PUBMED   2166941
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AW919077.1, L13245.1, BF285251.1 and BQ780519.1.
            
            On Oct 6, 2004 this sequence version replaced NM_078622.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M36071.1, BC085713.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-589               AW919077.1         2-590
            590-1204            L13245.1           487-1101
            1205-1371           BF285251.1         166-332             c
            1372-1567           BQ780519.1         1-196               c
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q22"
     gene            1..1567
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /note="phosphate cytidylyltransferase 1A, choline"
                     /db_xref="GeneID:140544"
                     /db_xref="RGD:70515"
     exon            1..93
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            94..220
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..1207
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /EC_number="2.7.7.15"
                     /note="choline-phosphate cytidylyltransferase A-like;
                     phosphate cytidylyltransferase 1, choline, alpha;
                     phosphorylcholine transferase A; choline phosphate
                     cytidylyltransferase 1 alpha; CT A; CCT A;
                     CTP:phosphocholine cytidylyltransferase A;
                     CTP:phosphocholine cytidylyl transferase;
                     CTP:phosphocholine cytidylyltransferase alpha"
                     /codon_start=1
                     /product="choline-phosphate cytidylyltransferase A"
                     /protein_id="NP_511177.2"
                     /db_xref="GeneID:140544"
                     /db_xref="RGD:70515"
                     /translation="MDAQSSAKVNSRKRRKEVPGPNGATEEDGIPSKVQRCAVGLRQP
                     APFSDEIEVDFSKPYVRVTMEEACRGTPCERPVRVYADGIFDLFHSGHARALMQAKNL
                     FPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAE
                     HRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYAR
                     RNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQ
                     KWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTHERSPSPSF
                     RWPFSGKTSPSSSPASLSRCKAVTCDISEDEED"
     exon            221..320
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            321..437
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            438..589
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            590..668
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            669..811
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            812..1000
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1551
                     /gene="Pcyt1a"
                     /gene_synonym="CCT-alpha; CTP"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccgggcgtc tccccgcaac ctatttgact tcgctagtcg gtgacgcggc gcggggaagg
       61 aatccgaggg gaccggagct tagaggagtt gagttaaaag aagatggatg cacagagttc
      121 agctaaagtc aattcaagga agaggaggaa agaggtacct ggccctaatg gagcaacaga
      181 ggaagatgga attccttcca aagtgcagcg ctgtgcagtt ggtttacggc agccagctcc
      241 tttttctgat gaaattgaag ttgactttag taagccctat gtcagggtga ctatggaaga
      301 agcctgcagg ggaactcctt gtgagcggcc tgtgagagtt tatgcggatg gaatatttga
      361 cttatttcac tctggtcacg cccgggctct gatgcaagca aagaaccttt tccctaatac
      421 atatctcatc gtgggagtct gcagtgatga gctaacgcac aacttcaagg gcttcactgt
      481 gatgaacgaa aatgaacgtt acgacgcggt gcagcactgc cgttacgtag acgaggtggt
      541 gaggaacgcc ccctggactc tgacgcccga gttcctggcg gagcaccgga ttgatttcgt
      601 cgcccatgac gatatcccct actcttcggc agggagcgac gatgtgtata agcacatcaa
      661 ggaagcaggc atgtttgctc ccacacagag gacagaaggc atctccacat cagacatcat
      721 cacccgcatt gtccgtgact atgatgtgta tgcaagacgg aacctgcaga gaggctacac
      781 tgccaaggag ctcaatgtca gctttatcaa cgaaaagaaa taccacttgc aagaacgagt
      841 tgataaggta aagaagaaag tgaaagatgt ggaggaaaag tcaaaagaat ttgtgcagaa
      901 agtggaggag aagagcatcg acctcatcca gaagtgggag gagaagtccc gagagttcat
      961 tggaagtttc ctggaaatgt ttggtccaga aggagcgctg aagcacatgc tgaaagaggg
     1021 aaaaggtcgg atgctgcagg ccatcagtcc caagcagagt cccagcagca gccctactca
     1081 tgagcgctcc ccctccccct cctttcggtg gcccttctct ggcaagactt ccccatcttc
     1141 ctccccagca agtctctcta ggtgcaaggc tgtgacctgt gacatcagcg aggatgaaga
     1201 ggactaactt ttcatccctg ttccctctct ctccccctgc ccgtcacctc cagaagctct
     1261 gtcgaattcc attttgtgat cccaacacta aacctaagga catctacaaa gaacagacaa
     1321 acggggcaag aggacctggg aacgggggaa ccaaaacagc ccatgccctg cctgagacat
     1381 tctcagccac accacggagg ctgcccacac cttagaagcc tgctctgcat ccatctttcc
     1441 tccccagaac gttgctttac tgtttattgc tcatgtaatc ttgacaggga gattgtcatt
     1501 tacatcattt cttttaaaaa gtagtctttc aaatatagta agtacaacta aaaaaaaaaa
     1561 aaaaaaa
//
