LOCUS       NM_199253               3350 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus proprotein convertase subtilisin/kexin type 9
            (Pcsk9), mRNA.
ACCESSION   NM_199253 XM_233261
VERSION     NM_199253.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Bao,H., Wang,X., Zhou,H., Zhou,W., Liao,F., Wei,F., Yang,S., Luo,Z.
            and Li,W.
  TITLE     PCSK9 regulates myofibroblast transformation through the JAK2/STAT3
            pathway to regulate fibrosis after myocardial infarction
  JOURNAL   Biochem Pharmacol 220, 115996 (2024)
   PUBMED   38154546
  REMARK    GeneRIF: PCSK9 regulates myofibroblast transformation through the
            JAK2/STAT3 pathway to regulate fibrosis after myocardial
            infarction.
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Ou-Yang,Y.N., Deng,F.F., Wang,Y.J., Chen,M., Yang,P.F., Yang,Z. and
            Tian,Z.
  TITLE     High-salt diet induces dyslipidemia through the SREBP2/PCSK9
            pathway in dahl salt-sensitive rats
  JOURNAL   Biochimie 216, 34-45 (2024)
   PUBMED   37844755
  REMARK    GeneRIF: High-salt diet induces dyslipidemia through the
            SREBP2/PCSK9 pathway in dahl salt-sensitive rats.
REFERENCE   3  (bases 1 to 3350)
  AUTHORS   Huang,G., Lu,X., Duan,Z., Zhang,K., Xu,L., Bao,H., Xiong,X.,
            Lin,M., Li,C., Li,Y., Zhou,H., Luo,Z. and Li,W.
  TITLE     PCSK9 Knockdown Can Improve Myocardial Ischemia/Reperfusion Injury
            by Inhibiting Autophagy
  JOURNAL   Cardiovasc Toxicol 22 (12), 951-961 (2022)
   PUBMED   36342620
  REMARK    GeneRIF: PCSK9 Knockdown Can Improve Myocardial
            Ischemia/Reperfusion Injury by Inhibiting Autophagy.
REFERENCE   4  (bases 1 to 3350)
  AUTHORS   Huang,G., Lu,X., Zhou,H., Li,R., Huang,Q., Xiong,X., Luo,Z. and
            Li,W.
  TITLE     PCSK9 inhibition protects against myocardial ischemia-reperfusion
            injury via suppressing autophagy
  JOURNAL   Microvasc Res 142, 104371 (2022)
   PUBMED   35460665
  REMARK    GeneRIF: PCSK9 inhibition protects against myocardial
            ischemia-reperfusion injury via suppressing autophagy.
REFERENCE   5  (bases 1 to 3350)
  AUTHORS   Schreckenberg,R., Wolf,A., Szabados,T., Gomori,K., Szabo,I.A.,
            Agoston,G., Brenner,G., Bencsik,P., Ferdinandy,P., Schulz,R. and
            Schluter,K.D.
  TITLE     Proprotein Convertase Subtilisin Kexin Type 9 (PCSK9) Deletion but
            Not Inhibition of Extracellular PCSK9 Reduces Infarct Sizes Ex Vivo
            but Not In Vivo
  JOURNAL   Int J Mol Sci 23 (12), 6512 (2022)
   PUBMED   35742954
  REMARK    GeneRIF: Proprotein Convertase Subtilisin Kexin Type 9 (PCSK9)
            Deletion but Not Inhibition of Extracellular PCSK9 Reduces Infarct
            Sizes Ex Vivo but Not In Vivo.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3350)
  AUTHORS   Naureckiene,S., Ma,L., Sreekumar,K., Purandare,U., Lo,C.F.,
            Huang,Y., Chiang,L.W., Grenier,J.M., Ozenberger,B.A.,
            Jacobsen,J.S., Kennedy,J.D., DiStefano,P.S., Wood,A. and Bingham,B.
  TITLE     Functional characterization of Narc 1, a novel proteinase related
            to proteinase K
  JOURNAL   Arch Biochem Biophys 420 (1), 55-67 (2003)
   PUBMED   14622975
  REMARK    GeneRIF: Our functional characterization of Narc 1 reinforces the
            inference drawn that this enzyme is most closely related to
            representatives of the proteinase K family, but it is also
            sufficiently different to warrant its classification in a separate
            sub-family.
REFERENCE   7  (bases 1 to 3350)
  AUTHORS   Abifadel,M., Varret,M., Rabes,J.P., Allard,D., Ouguerram,K.,
            Devillers,M., Cruaud,C., Benjannet,S., Wickham,L., Erlich,D.,
            Derre,A., Villeger,L., Farnier,M., Beucler,I., Bruckert,E.,
            Chambaz,J., Chanu,B., Lecerf,J.M., Luc,G., Moulin,P.,
            Weissenbach,J., Prat,A., Krempf,M., Junien,C., Seidah,N.G. and
            Boileau,C.
  TITLE     Mutations in PCSK9 cause autosomal dominant hypercholesterolemia
  JOURNAL   Nat Genet 34 (2), 154-156 (2003)
   PUBMED   12730697
REFERENCE   8  (bases 1 to 3350)
  AUTHORS   Seidah,N.G., Benjannet,S., Wickham,L., Marcinkiewicz,J.,
            Jasmin,S.B., Stifani,S., Basak,A., Prat,A. and Chretien,M.
  TITLE     The secretory proprotein convertase neural apoptosis-regulated
            convertase 1 (NARC-1): liver regeneration and neuronal
            differentiation
  JOURNAL   Proc Natl Acad Sci U S A 100 (3), 928-933 (2003)
   PUBMED   12552133
  REMARK    GeneRIF: NARC-1 is a proteinase K-like subtilase with a role in the
            differentiation of cortical neurons.
REFERENCE   9  (bases 1 to 3350)
  AUTHORS   Paez-Pereda,M., Giacomini,D., Refojo,D., Nagashima,A.C.,
            Hopfner,U., Grubler,Y., Chervin,A., Goldberg,V., Goya,R.,
            Hentges,S.T., Low,M.J., Holsboer,F., Stalla,G.K. and Arzt,E.
  TITLE     Involvement of bone morphogenetic protein 4 (BMP-4) in pituitary
            prolactinoma pathogenesis through a Smad/estrogen receptor
            crosstalk
  JOURNAL   Proc Natl Acad Sci U S A 100 (3), 1034-1039 (2003)
   PUBMED   12552124
REFERENCE   10 (bases 1 to 3350)
  AUTHORS   Petkov,P.M., Kim,K., Sandhu,J., Shafritz,D.A. and Dabeva,M.D.
  TITLE     Identification of differentially expressed genes in epithelial
            stem/progenitor cells of fetal rat liver
  JOURNAL   Genomics 68 (2), 197-209 (2000)
   PUBMED   10964518
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY847775.1.
            
            On Oct 5, 2005 this sequence version replaced NM_199253.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY847775.1, BC133063.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q34"
     gene            1..3350
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /note="proprotein convertase subtilisin/kexin type 9"
                     /db_xref="GeneID:298296"
                     /db_xref="RGD:728909"
     exon            1..394
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..2266
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /note="neural apoptosis-regulated convertase 1; proprotein
                     convertase PC9; subtilisin/kexin-like protease PC9;
                     proprotein convertase 9"
                     /codon_start=1
                     /product="proprotein convertase subtilisin/kexin type 9
                     precursor"
                     /protein_id="NP_954862.2"
                     /db_xref="GeneID:298296"
                     /db_xref="RGD:728909"
                     /translation="MGIRCSTWLRWPLSPQLLLLLLLCPTGSRAQDEDGDYEELMLAL
                     PSQEDSLVDEASHVATATFRRCSKEAWRLPGTYVVVLMEETQRLQVEQTAHRLQTWAA
                     RRGYVIKVLHVFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSLVFAQSIPWNLERI
                     IPAWQQTEEDSSPDGSSQVEVYLLDTSIQSGHREIEGRVTITDFNSVPEEDGTRFHRQ
                     ASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLI
                     QPSGPLVVLLPLAGGYSRILNTACQRLARTGVVLVAAAGNFRDDACLYSPASAPEVIT
                     VGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCYMSQSGTSQAAAHVA
                     GIVAMMLNRDPALTLAELRQRLILFSTKDVINMAWFPEDQRVLTPNRVATLPPSTQET
                     GGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDRIEAIGGQQVC
                     KALNAFGGEGVYAVARCCLLPRVNCSIHNTPAARAGPQTPVHCHQKDHVLTGCSFHWE
                     VENLRAQQQPLLRSRHQPGQCVGHQEASVHASCCHAPGLECKIKEHGIAGPAEQVTVA
                     CEAGWTLTGCNVLPGASLPLGAYSVDNVCVARIRDAGRADRTSEEATVAAAICCRSRP
                     SAKASWVHQ"
     exon            395..586
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            587..710
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            711..844
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            845..986
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1183
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1367
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1541
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1690
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1868
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..2050
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..3333
                     /gene="Pcsk9"
                     /gene_synonym="NARC-1; Narc1; PC9"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acaccctaaa aggctttcca tctttatgtg gacgcgcagt ctgccactgg gctcccgttc
       61 tctctctgtc tgagacctga ggactgagcc agtgcttggc tcccctaggg acatcacagc
      121 ctgcagtccc agagcccagt gccccgcgtt cacgtccttc ccgaggcctc gtgcacctct
      181 cctcgccccg atgggcatcc gctgctctac atggttgcgg tggccgctgt cgccgcagct
      241 gctgttgttg ctgctactgt gccccacagg ctcccgtgcc caggacgagg acggagacta
      301 cgaagagctg atgctcgccc tcccgtccca ggaggatagc ctggttgatg aggcctcaca
      361 cgtggccacc gccaccttcc gccgttgctc caaggaggcc tggaggctgc caggaaccta
      421 cgtggtggtg ctgatggagg agacccagcg gctgcaggtt gaacaaactg cccatcgcct
      481 gcagacctgg gcggcccgcc ggggctatgt catcaaggtt ctgcatgtct tttatgacct
      541 cttccctggc ttcttggtga agatgagcag tgacctgttg ggcctggccc tgaagttgcc
      601 ccatgtggag tacatcgagg aagactcatt agtcttcgcc cagagcatcc catggaacct
      661 ggagcggatt atcccagcgt ggcagcagac agaggaagat agctcccctg acggaagtag
      721 ccaggtggag gtgtatctct tagataccag catccagagt ggccaccggg agatcgaggg
      781 cagagttacc atcactgact tcaacagtgt gcctgaggag gacgggacac gtttccacag
      841 acaggcgagc aagtgtgaca gccatggcac ccacctagca ggtgtggtca gcggccggga
      901 tgctggtgtg gccaagggca ccagtctgca cagtctgcgt gtgctcaact gtcaagggaa
      961 gggcacagtc agcggcaccc tcataggcct ggagtttatt cggaagagcc agctaatcca
     1021 gccttcgggg ccactcgtgg tgctgctgcc cctggcgggt gggtatagcc ggatccttaa
     1081 cactgcctgc cagcgcctgg caaggactgg ggtagtgctg gtggcagcag ctgggaattt
     1141 ccgagatgat gcctgcctct actccccagc ctctgctcca gaggtcatta cagttggggc
     1201 cactaatgcc caggaccagc cagtcaccct ggggactttg gggacaaact ttggacgctg
     1261 tgtggatctc tttgcccccg ggaaggacat catcggagcc tccagtgact gtagcacgtg
     1321 ctacatgtca cagagtggga cgtcacaagc tgctgcccac gtggctggca ttgtggctat
     1381 gatgctgaac cgggatccag cacttaccct ggctgagctg cggcagaggt tgatcctctt
     1441 ctctaccaaa gatgtcatca acatggcctg gttccctgag gaccagcggg tgctgacccc
     1501 caaccgggtg gccacactgc cccccagcac ccaggagaca ggcgggcagc tgctctgccg
     1561 gacagtgtgg tccgcccact cagggcccac ccgtacagca acagccacag cccgctgtgc
     1621 ccctgaagag gaactgctga gctgctccag cttctccagg agcgggaggc gacggggtga
     1681 tcgaattgag gccatagggg gccagcaggt ctgcaaggcc ctcaatgcat ttgggggtga
     1741 gggtgtctat gctgtcgcaa ggtgctgcct gcttccccgt gtcaactgca gcatccacaa
     1801 cactcctgca gccagagctg gtccgcagac ccccgtccac tgccaccaga aggaccatgt
     1861 tctcacaggc tgcagcttcc actgggaagt ggaaaacctt agagcccagc agcagcctct
     1921 gctgaggtcc agacatcaac ctggccaatg cgttggccac caggaggcca gtgtccacgc
     1981 ttcctgctgc catgctccag gtctggaatg caaaatcaag gagcatggca tcgcaggtcc
     2041 tgcagagcag gtcaccgtgg cctgtgaggc aggatggacc ctgactggat gcaacgttct
     2101 ccctggggca tccctccctc tgggggccta cagtgtggac aacgtgtgtg tggcacgaat
     2161 ccgtgatgct ggtagagcgg acaggaccag tgaagaagcc acggtagctg ctgccatctg
     2221 ctgccggagc cggccttcgg caaaggcctc ctgggttcac cagtgacagc ctcaggcagg
     2281 cattgtacct gtggctggac gcagagatgg acgtcctggc tctcttgtgt ctagccaaag
     2341 gtggggagac tctgcctggg ggaacttggc gtctcatcct gggtacccat tcctggtgta
     2401 tgtgtgggga agcacctcct tcatggtcag ggggcctgtg cttggccttc tgccatcgaa
     2461 gatgttaagc tatagttggc tttggccagc tgctccagta tatcagaacc tgagagcact
     2521 tgctacaagg ctagtgttca ggccttaggc ctccagagtg aatgtatcct gcaggaagat
     2581 aatgatggat cgtgaccctt gacggtcacc cccctccccc aggtcagatg tcaccagact
     2641 agaacagtat ctgaaagctg ctggggccac tcacagcttg cttactctgg agacagcatt
     2701 ttgggctccc tgattaatgc agatcagttc tgcccacctc caggggtgga tccagctgtg
     2761 aggctcacct gtatcttcca gatgttctca tctgctgcac cgaaggctct ggccctgctc
     2821 aggagaacac gctacgaact cctagctgac tctgtttgca ctggagaacc acacagggct
     2881 taccccacta ccctgtgcac tgactggctt cactttatga aggaagagac agggccagag
     2941 aagcaatgtc atgcagccag tgatgctagg acataaatcc agagtggctg gccctgaagc
     3001 catgcctctt ggcaatgcca ggctgggcat cctatttttg aagcaaacaa aaaatgagag
     3061 gacaggctgt gcttcagcgg cttgttcctg gacctatgct cccttagccc cagtcccacg
     3121 gattatgtgg agagtggagg agcaacagag ggcgactgta ctaaggccac acaagtcgac
     3181 aagaacacct atatcctttt gacctcttct gcttttttat agtaagcttt ccctacctgc
     3241 gttgcttttg taactcgata tttatgccgg gttttataga gtttttatta tgtagtgact
     3301 tttcagaata aaagaagctg atgtgactgc ctgaaaaaaa aaaaaaaaaa
//
