LOCUS       NM_012999               4154 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus proprotein convertase subtilisin/kexin type 6
            (Pcsk6), mRNA.
ACCESSION   NM_012999
VERSION     NM_012999.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Jiang,H., Wang,L. and Pan,J.
  TITLE     Inhibition of pro-protein convertase subtilisin/kexin type 6 has a
            protective role against synovitis in a rat model of rheumatoid
            arthritis
  JOURNAL   Mol Med Rep 12 (5), 7681-7686 (2015)
   PUBMED   26458419
  REMARK    GeneRIF: PCSK6 knockdown significantly decreased the cell
            proliferation, invasion and migration of fibroblastlike
            synoviocytes from rats with collageninduced arthritis.
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Essalmani,R., Susan-Resiga,D., Chamberland,A., Asselin,M.C.,
            Canuel,M., Constam,D., Creemers,J.W., Day,R., Gauthier,D., Prat,A.
            and Seidah,N.G.
  TITLE     Furin is the primary in vivo convertase of angiopoietin-like 3 and
            endothelial lipase in hepatocytes
  JOURNAL   J Biol Chem 288 (37), 26410-26418 (2013)
   PUBMED   23918928
REFERENCE   3  (bases 1 to 4154)
  AUTHORS   Akamatsu,T., Azlina,A., Purwanti,N., Karabasil,M.R., Hasegawa,T.,
            Yao,C. and Hosoi,K.
  TITLE     Inhibition and transcriptional silencing of a subtilisin-like
            proprotein convertase, PACE4/SPC4, reduces the branching
            morphogenesis of and AQP5 expression in rat embryonic submandibular
            gland
  JOURNAL   Dev Biol 325 (2), 434-443 (2009)
   PUBMED   19013448
  REMARK    GeneRIF: PACE4 regulates the SMG development via the activation of
            some growth/differentiation factors.
REFERENCE   4  (bases 1 to 4154)
  AUTHORS   Akamatsu,T., Purwanti,N., Karabasil,M.R., Li,X., Yao,C.,
            Kanamori,N. and Hosoi,K.
  TITLE     Temporospatially regulated expression of subtilisin-like proprotein
            convertase PACE4 (SPC4) during development of the rat submandibular
            gland
  JOURNAL   Dev Dyn 236 (1), 314-320 (2007)
   PUBMED   17083113
  REMARK    GeneRIF: temporospatially regulated expression of PACE4 suggests
            its apparent association with the proliferation, differentiation,
            and establishment of functional acinar and ductal cells of the
            submandibular gland.
REFERENCE   5  (bases 1 to 4154)
  AUTHORS   Tsuji,A., Kikuchi,Y., Sato,Y., Koide,S., Yuasa,K., Nagahama,M. and
            Matsuda,Y.
  TITLE     A proteomic approach reveals transient association of
            reticulocalbin-3, a novel member of the CREC family, with the
            precursor of subtilisin-like proprotein convertase, PACE4
  JOURNAL   Biochem J 396 (1), 51-59 (2006)
   PUBMED   16433634
  REMARK    GeneRIF: Selective and transient association of RCN-3 with the
            precursor of PACE4 plays an important role in the biosynthesis of
            PACE4.
REFERENCE   6  (bases 1 to 4154)
  AUTHORS   Schaner,P., Todd,R.B., Seidah,N.G. and Nillni,E.A.
  TITLE     Processing of prothyrotropin-releasing hormone by the family of
            prohormone convertases
  JOURNAL   J Biol Chem 272 (32), 19958-19968 (1997)
   PUBMED   9242664
REFERENCE   7  (bases 1 to 4154)
  AUTHORS   Zhong,M., Benjannet,S., Lazure,C., Munzer,S. and Seidah,N.G.
  TITLE     Functional analysis of human PACE4-A and PACE4-C isoforms:
            identification of a new PACE4-CS isoform
  JOURNAL   FEBS Lett 396 (1), 31-36 (1996)
   PUBMED   8906861
REFERENCE   8  (bases 1 to 4154)
  AUTHORS   Seidah,N.G., Benjannet,S., Pareek,S., Savaria,D., Hamelin,J.,
            Goulet,B., Laliberte,J., Lazure,C., Chretien,M. and Murphy,R.A.
  TITLE     Cellular processing of the nerve growth factor precursor by the
            mammalian pro-protein convertases
  JOURNAL   Biochem J 314 (Pt 3) (Pt 3), 951-960 (1996)
   PUBMED   8615794
REFERENCE   9  (bases 1 to 4154)
  AUTHORS   Johnson,R.C., Darlington,D.N., Hand,T.A., Bloomquist,B.T. and
            Mains,R.E.
  TITLE     PACE4: a subtilisin-like endoprotease prevalent in the anterior
            pituitary and regulated by thyroid status
  JOURNAL   Endocrinology 135 (3), 1178-1185 (1994)
   PUBMED   8070361
REFERENCE   10 (bases 1 to 4154)
  AUTHORS   Rehemtulla,A., Barr,P.J., Rhodes,C.J. and Kaufman,R.J.
  TITLE     PACE4 is a member of the mammalian propeptidase family that has
            overlapping but not identical substrate specificity to PACE
  JOURNAL   Biochemistry 32 (43), 11586-11590 (1993)
   PUBMED   8218226
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Nov 27, 2020 this sequence version replaced NM_012999.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L31894.1, SRR26643289.1845.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-274               JAXUCZ010000001.1  128839742-128840015
            275-379             JAXUCZ010000001.1  128887437-128887541
            380-484             JAXUCZ010000001.1  128910726-128910830
            485-628             JAXUCZ010000001.1  128911369-128911512
            629-705             JAXUCZ010000001.1  128912724-128912800
            706-794             JAXUCZ010000001.1  128915042-128915130
            795-967             JAXUCZ010000001.1  128935480-128935652
            968-1180            JAXUCZ010000001.1  128941959-128942171
            1181-1281           JAXUCZ010000001.1  128945725-128945825
            1282-1385           JAXUCZ010000001.1  128952312-128952415
            1386-1503           JAXUCZ010000001.1  128953842-128953959
            1504-1692           JAXUCZ010000001.1  128962659-128962847
            1693-1829           JAXUCZ010000001.1  128966225-128966361
            1830-2009           JAXUCZ010000001.1  129013005-129013184
            2010-2112           JAXUCZ010000001.1  129019406-129019508
            2113-2309           JAXUCZ010000001.1  129021343-129021539
            2310-2397           JAXUCZ010000001.1  129023118-129023205
            2398-2501           JAXUCZ010000001.1  129027199-129027302
            2502-2631           JAXUCZ010000001.1  129031253-129031382
            2632-2744           JAXUCZ010000001.1  129035254-129035366
            2745-4154           JAXUCZ010000001.1  129036648-129038057
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..4154
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /note="proprotein convertase subtilisin/kexin type 6"
                     /db_xref="GeneID:25507"
                     /db_xref="RGD:3246"
     exon            1..274
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..2842
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /note="kexin-like protease PC7A; subtilisin/kexin-like
                     protease PACE4; subtilisin-like proprotein convertase 4;
                     paired basic amino acid cleaving enzyme 4; Subtilisin -
                     like endoprotease"
                     /codon_start=1
                     /product="proprotein convertase subtilisin/kexin type 6
                     precursor"
                     /protein_id="NP_037131.2"
                     /db_xref="GeneID:25507"
                     /db_xref="RGD:3246"
                     /translation="MPPRAPPAPGPRPPPRAAGRHGLSPLAPRPWRWLLLLALPAVCS
                     ALPPPRPVYTNHWAVQVLGGPGAADRVAAAHGYLNLGQIGNLDDYYHFYHSKTFKRST
                     LSSRGPHTFLRMDPQVKWLQQQEVKRRVKRQARSDSLYFNDPIWSNMWYMHCADKNSR
                     CRSEMNVQAAWKRGYTGKNVVVTILDDGIERNHPDLAPNYDSYASYDVNGNDYDPSPR
                     YDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLDGDVTDVVEAKSLGIR
                     PNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGREGD
                     HCSCDGYTNSIYTISVSSTTENGHKPWYLEECASTLATTYSSGAFYERKIVTTDLRQR
                     CTDGHTGTSVSAPMVAGIIALALEANNQLTWRDVQHLLVKTSRPAHLKASDWKVNGAG
                     HKVSHLYGFGLVDAEALVLEARKWTAVPSQHMCVATADKRPRSIPVVQVLRTTALTNA
                     CADHSDQRVVYLEHVVVRISISHPRRGDLQIHLISPSGTKSQLLAKRLLDFSNEGFTN
                     WEFMTVHCWGEKAEGEWTLEVQDIPSQVRNPEKQGKLKEWSLILYGTAEHPYRTFSSH
                     QSRSRMLELSVPEQEPLKAEGPPPQAETPEEEEEYTGVCHPECGDKGCDGPSADQCLN
                     CVHFSLGNSKTNRKCVSECPLGYFGDTAARRCRRCHKGCETCTGRSPTQCLSCRRGFY
                     HHQETNTCVTLCPAGLYADESQRLCLRCHPSCQKCVDEPEKCTVCKEGFSLARGSCIP
                     DCEPGTYFDSELIRCGECHHTCRTCVGPSREECIHCAKSFHFQDWKCVPACGEGFYPE
                     EMPGLPHKVCRRCDENCLSCEGSSRNCSRCKAGFTQLGTSCITNHTCSNADETFCEMV
                     KSNRLCERKLFIQFCCRTCLLAG"
     exon            275..379
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            380..484
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            485..628
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            629..705
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            706..794
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            795..967
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1180
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1281
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1385
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1503
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1692
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1829
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..2009
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2112
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2309
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2397
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2501
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2631
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2744
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
     exon            2745..4154
                     /gene="Pcsk6"
                     /gene_synonym="Pace4; Spc4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcgggcgccg cgcgagcctg ccgctgccat gcctccgcgc gcgccgccag cgcccgggcc
       61 ccggccgccg ccccgggccg ccggccggca cgggctctcg ccgctggcgc cgcgcccctg
      121 gcgttggctg cttctgctcg ctctgcccgc cgtctgctcc gcgctgccgc cgccgcgccc
      181 cgtctacacc aaccactggg cagtgcaagt gctgggcggc cccggcgcgg cggaccgcgt
      241 ggctgcggct cacggctacc tcaacttggg ccagattgga aatctggacg attactatca
      301 tttttaccac agcaagacct tcaagagatc aaccttgagt agcaggggcc cccacacctt
      361 cctcagaatg gacccacagg taaaatggct ccagcaacag gaagtgaaac gcagggtcaa
      421 gagacaggcg cgaagcgact ctctttattt caatgatccc atttggtcca acatgtggta
      481 tatgcattgt gctgataaga acagtcgctg tcggtcagag atgaacgtcc aggcggcatg
      541 gaagcgcggc tacacaggaa agaacgtggt tgtcaccatc ctcgatgacg gcatagaaag
      601 gaatcaccca gacctggccc ccaactacga ttcctatgca agctacgatg tcaacggaaa
      661 cgattatgac ccatcaccga gatatgatgc cagcaacgag aacaaacatg gtactcgctg
      721 tgcgggagaa gtcgctgctt cagccaacaa ctcctactgc atcgtgggca tagcatataa
      781 tgcaaagata ggaggcatcc ggatgctgga cggtgacgtg accgacgtgg ttgaggccaa
      841 gtctctgggc atcagaccca actacattga catttacagc gctagttggg ggccagatga
      901 tgatgggaag accgtggatg ggcccggccg tctggctaaa caggctttcg agtatggcat
      961 taaaaagggc cgccaaggtc tgggctccat ttttgtctgg gcctctggga atggtgggag
     1021 agaaggggac cactgctcct gtgatggcta caccaacagc atctacacca tctctgtgag
     1081 cagcaccact gagaacggcc acaaaccctg gtacctggag gaatgtgctt ccaccttggc
     1141 taccacctac agcagcgggg ccttctatga acggaagatc gtcaccacgg acctgcgtca
     1201 gcgctgcacc gacggccaca ctgggacatc tgtctcagct cccatggtgg ctggcatcat
     1261 tgccctggct ctagaagcaa acaaccagtt gacctggagg gacgtgcagc acctgttagt
     1321 aaagacgtca cggccggctc atctgaaggc gagtgactgg aaagtcaacg gagctgggca
     1381 taaagttagc catctctatg gatttggctt ggtggatgct gaagcgctcg tcctagaggc
     1441 aaggaagtgg acggcagtgc catcccagca catgtgcgtg gccaccgcag acaaaaggcc
     1501 caggagcatc cccgtagtgc aggtgctgcg gaccacagcc ctgaccaatg cctgtgcaga
     1561 ccactctgac cagcgtgtgg tgtacctgga gcatgtggta gtccgaatct ctatctcaca
     1621 tccacgacgg ggtgacctcc agatccacct gatttctccc tctggaacca agtctcaact
     1681 tttggcaaag agattgctgg atttttccaa tgaggggttc acgaactggg agttcatgac
     1741 tgtccactgc tggggagaaa aggctgaagg tgaatggacc ctggaagtcc aggatatacc
     1801 atcgcaggtc cgcaacccag agaaacaagg aaagttgaaa gaatggagcc tcattttata
     1861 tggcactgca gagcacccat accgcacctt cagctcccac cagtctcgct cacggatgct
     1921 ggagctttca gtcccggaac aggagcctct caaggctgag ggaccaccac cgcaggcaga
     1981 gactccagaa gaagaggaag agtacacagg tgtgtgccat ccagagtgtg gtgataaagg
     2041 ctgcgatggt cccagtgcag accagtgctt gaactgcgtc cacttcagcc tgggaaactc
     2101 caagacaaac aggaagtgtg tgagcgagtg ccccttgggc tactttgggg acacagcagc
     2161 aagacgctgc cgtcgatgcc ataagggatg tgagacatgc acgggcagga gcccaacaca
     2221 gtgcctgtct tgtcgccgtg ggttctatca ccaccaggaa acgaacacat gtgtgaccct
     2281 gtgtcctgcc ggactttatg ctgatgaaag tcagagactc tgcctcaggt gccacccgag
     2341 ctgtcagaag tgtgtggatg aacctgagaa gtgcactgtg tgcaaggagg gattcagcct
     2401 cgcacggggc agctgcattc cggactgtga accaggtacc tacttcgatt ctgagctcat
     2461 cagatgtggg gaatgccatc acacctgccg gacctgcgtg gggcccagca gagaagaatg
     2521 tattcactgt gcaaaaagct tccacttcca agactggaaa tgtgtgccgg cctgtggtga
     2581 gggcttctac ccggaggaga tgcctggctt accccacaaa gtgtgtcgaa gatgtgatga
     2641 aaactgcctg agctgcgagg gctccagcag gaactgcagc agatgtaaag ctggcttcac
     2701 gcagctgggg acctcctgca tcaccaacca cacgtgcagt aacgccgatg agaccttctg
     2761 cgagatggta aagtccaacc ggctctgtga acggaagctc ttcatccagt tttgttgccg
     2821 cacctgcctc ctggctgggt agggcgggcg ccagctgcca cagagggcag ggtcctcctg
     2881 tctgcccttt tgcccagcta ccttcctaca gatggccagc catagcccat tccttggggt
     2941 ggccctgagt ctgacagctg tgccctcccc cccccagagc tgggtcccac tgcagcatct
     3001 ctgagcacct gaactaggtg gaggtggccc ttaaggataa ggctaaatcg gcaaaaatcc
     3061 ccctgaactc tgcttgctgg ctgcagtcta aagctggact cgaaatagga acagagtgaa
     3121 ttatgagact catgcctgca gcttgggagt ggcttctggg accctagttt actgaaactt
     3181 caagaccaaa gcagaaaaag agagatgcct ggcatcccat caagtcctcc tcccacacat
     3241 tcgtgtgacc gtgacagatc tcaccgagtt ggctggcagg accccatgct gtcctcacct
     3301 ataatgaagg gcctcgcttc ctccccatgc atcactggcc accaaacagc ctgagggatg
     3361 gtttgatgag actgtaaata aaataggttt cagggcataa gatgtatgac cactggggat
     3421 agaacctatg tctacacagc tccttccgaa actacagccc cctgactgga aggtccggca
     3481 ccagactgaa gtaggtctac tcatcctctc ctcagcattc tcctctgagt gagctgagct
     3541 gtccaagtga ctgttcaacc tgtgtcccag gggcctcctg ggcctgagcc accagtcatc
     3601 tacagataca gagcctgtgg aggagggtcc aaaggagcta cttaaggcta gccgaaagac
     3661 ctctaatggc caagcagttc ctccttatgc aaagccagcc ccaaatcact aatcgccagc
     3721 cctccatggc acacaactgc ttctcaagtg catttggcct ccacactcag gactctgttc
     3781 tcgggtggac actgctctgg cccagtatag tacaagccta cgttgataga gctggattga
     3841 tttttctgcc aagcctgtgt gggcatttta taagctacgt gttctaattt ttaccgatgt
     3901 taattatttt gacaaatatt tcatatattt tcattgaaat gcgcagatct gcttggtcca
     3961 gttcccttta acgtgggaat aacatttgcc ttaaattttt ccaacctcgt ctctctccat
     4021 atggtcctgc tctcctctct gaatataatg tgttttgtct tgtcacctgt aagtggcaag
     4081 gactcagctg ttgtctgttg aatccacaac ttcaaataag aaatcagtga agcaaatcta
     4141 atgttaaccc tgag
//
