LOCUS       NM_133559               2458 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus proprotein convertase subtilisin/kexin type 4
            (Pcsk4), mRNA.
ACCESSION   NM_133559
VERSION     NM_133559.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Kang,B.W., Jeon,H.S., Chae,Y.S., Lee,S.J., Park,J.S., Choi,G.S. and
            Kim,J.G.
  TITLE     Impact of Genetic Variation in MicroRNA-binding Site on
            Susceptibility to Colorectal Cancer
  JOURNAL   Anticancer Res 36 (7), 3353-3361 (2016)
   PUBMED   27354594
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Iamsaard,S., Vanichviriyakit,R., Hommalai,G., Saewu,A., Srakaew,N.,
            Withyachumnarnkul,B., Basak,A. and Tanphaichitr,N.
  TITLE     Enzymatic activity of sperm proprotein convertase is important for
            mammalian fertilization
  JOURNAL   J Cell Physiol 226 (11), 2817-2826 (2011)
   PUBMED   21302280
REFERENCE   3  (bases 1 to 2458)
  AUTHORS   Gyamera-Acheampong,C., Sirois,F., Denis,N.J., Mishra,P., Figeys,D.,
            Basak,A. and Mbikay,M.
  TITLE     The precursor to the germ cell-specific PCSK4 proteinase is
            inefficiently activated in transfected somatic cells: evidence of
            interaction with the BiP chaperone
  JOURNAL   Mol Cell Biochem 348 (1-2), 43-52 (2011)
   PUBMED   21080038
  REMARK    GeneRIF: Maturation by propeptide removal occurs very inefficiently
            when rat or human proPCSK4 is overexpressed in HEK293 cells.
REFERENCE   4  (bases 1 to 2458)
  AUTHORS   Gyamera-Acheampong,C., Vasilescu,J., Figeys,D. and Mbikay,M.
  TITLE     PCSK4-null sperm display enhanced protein tyrosine phosphorylation
            and ADAM2 proteolytic processing during in vitro capacitation
  JOURNAL   Fertil Steril 93 (4), 1112-1123 (2010)
   PUBMED   19342015
REFERENCE   5  (bases 1 to 2458)
  AUTHORS   Gyamera-Acheampong,C., Tantibhedhyangkul,J., Weerachatyanukul,W.,
            Tadros,H., Xu,H., van de Loo,J.W., Pelletier,R.M., Tanphaichitr,N.
            and Mbikay,M.
  TITLE     Sperm from mice genetically deficient for the PCSK4 proteinase
            exhibit accelerated capacitation, precocious acrosome reaction,
            reduced binding to egg zona pellucida, and impaired fertilizing
            ability
  JOURNAL   Biol Reprod 74 (4), 666-673 (2006)
   PUBMED   16371590
REFERENCE   6  (bases 1 to 2458)
  AUTHORS   Qiu,Q., Basak,A., Mbikay,M., Tsang,B.K. and Gruslin,A.
  TITLE     Role of pro-IGF-II processing by proprotein convertase 4 in human
            placental development
  JOURNAL   Proc Natl Acad Sci U S A 102 (31), 11047-11052 (2005)
   PUBMED   16040806
REFERENCE   7  (bases 1 to 2458)
  AUTHORS   Mbikay,M., Tadros,H., Ishida,N., Lerner,C.P., De Lamirande,E.,
            Chen,A., El-Alfy,M., Clermont,Y., Seidah,N.G., Chretien,M.,
            Gagnon,C. and Simpson,E.M.
  TITLE     Impaired fertility in mice deficient for the testicular germ-cell
            protease PC4
  JOURNAL   Proc Natl Acad Sci U S A 94 (13), 6842-6846 (1997)
   PUBMED   9192653
REFERENCE   8  (bases 1 to 2458)
  AUTHORS   Seidah,N.G., Day,R., Hamelin,J., Gaspar,A., Collard,M.W. and
            Chretien,M.
  TITLE     Testicular expression of PC4 in the rat: molecular diversity of a
            novel germ cell-specific Kex2/subtilisin-like proprotein convertase
  JOURNAL   Mol Endocrinol 6 (10), 1559-1570 (1992)
   PUBMED   1448111
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from L14937.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L14937.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..2458
                     /gene="Pcsk4"
                     /note="proprotein convertase subtilisin/kexin type 4"
                     /db_xref="GeneID:171085"
                     /db_xref="RGD:620325"
     CDS             1..1965
                     /gene="Pcsk4"
                     /note="PC4; NEC 3; prohormone convertase 3; KEX2-like
                     endoprotease 3; neuroendocrine convertase 3"
                     /codon_start=1
                     /product="proprotein convertase subtilisin/kexin type 4
                     precursor"
                     /protein_id="NP_598243.1"
                     /db_xref="GeneID:171085"
                     /db_xref="RGD:620325"
                     /translation="MRPSQTALWLGLVLSLALLAVGWASARPPIYVSSWAVRVTKGYQ
                     EAERLARKFGFVNLGQIFPDDQYFHLRHRGVAQQSLTPHWGHRLRLKKEPKVRWFEQQ
                     TLRRRVKRSLVVPTDPWFSKQWYMNKEIEQDLNILKVWNQGLTGRGVVVSILDDGIEK
                     DHPDLWANYDPLASYDFNDYDPDPQPRYTPNDENRHGTRCAGEVSATANNGFCGAGVA
                     FNARIGGVRMLDGAITDIVEAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGLLTQEAF
                     RRGVTKGRQGLGTLFIWASGNGGLHYDNCNCDGYTNSIHTLSVGSTTRQGRVPWYSEA
                     CASTFTTTFSSGVVTDPQIVTTDLHHQCTDKHTGTSASAPLAAGMIALALEANPLLTW
                     RDLQHLVVRASRPAQLQAEDWRINGVGRQVSHHYGYGLLDAGLLVDLARVWLPTKPQK
                     KCTIRVVHTPTPILPRMLVPKNVTVCCDGSRRRLIRSLEHVQVQLSLSYSRRGDLEIF
                     LTSPMGTRSTLVAIRPLDISGQGYNNWIFMSTHYWDEDPQGLWTLGLENKGYYYNTGT
                     LYYCTLLLYGTAEDMTARPQTPQVTSCAHACAEGHRGAVPGKSLSPLHCGRTLPHLQQ
                     AVVVALQPHTAASDQGTGQLSPSYHTCSAA"
     exon            1..180
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            181..285
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            286..378
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            379..507
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            508..584
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            585..673
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            674..846
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            847..1059
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1160
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1264
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1382
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1568
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1693
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1820
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..2445
                     /gene="Pcsk4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgcggccct cccagacagc gctgtggctg ggtctggttt tgtctttggc cctcctggct
       61 gtggggtggg cctcagcccg accacccatc tatgtcagca gctgggcagt gcgggtgacc
      121 aaaggttacc aggaggctga gcgcctggca cgtaaatttg gcttcgtcaa cctgggacag
      181 atctttcctg atgaccagta tttccatctg aggcaccggg gtgtggccca gcagtccctg
      241 actccgcact ggggccaccg tctgcgcctg aagaaagagc ccaaggtgcg gtggtttgag
      301 cagcagactt tgaggcggcg ggtgaagcgc tccctggtgg tacccacaga cccctggttt
      361 tccaagcagt ggtacatgaa caaggagata gaacaagatc tcaacatcct aaaggtttgg
      421 aaccagggac tgactggccg gggagtggtg gtctccatct tggatgatgg cattgagaag
      481 gaccatccgg acctctgggc taattatgac cccctggcca gctatgactt caatgattac
      541 gacccagatc cccagcctcg atacacaccc aacgatgaga accggcatgg aacacgctgc
      601 gctggggagg tgtctgccac agcaaacaac ggtttctgtg gtgccggtgt ggccttcaat
      661 gccagaattg gaggcgtgcg catgttggat ggagccatca ctgacatcgt ggaggctcag
      721 tccctcagcc tgcagccgca acacatacac atctatagcg ccagttgggg ccccgaggat
      781 gatgggcgca cagtggacgg acccggcctc ctcacgcagg aggccttcag gcgtggtgta
      841 accaagggcc gccaagggct gggcacgctg ttcatctggg cctcgggaaa cggtggcctc
      901 cactacgaca actgcaattg tgacggctac accaacagca tccacacgct gtcagtgggc
      961 agtaccacgc ggcagggccg agtgccctgg tacagcgagg cctgcgcctc cacgttcacc
     1021 accaccttca gcagcggtgt ggtcaccgac ccacagatcg tcaccacgga cctacaccat
     1081 caatgcaccg acaagcacac gggcacctcg gcctccgccc cgctggccgc tggcatgatc
     1141 gccctggcgc tggaggccaa cccgctgctg acctggaggg acctgcagca cctggtggtc
     1201 cgcgcgtcca ggccggcgca gctgcaggcg gaggactgga ggatcaacgg cgtggggcgc
     1261 caagtgagcc accactatgg ctatgggctg ctggacgcgg ggctgctggt agacctggct
     1321 cgcgtgtggc tccctactaa gcctcagaag aaatgcacca ttcgggtggt gcacacccca
     1381 acccccatcc tgcctcggat gctggtgcca aagaacgtga ctgtatgctg cgatggctcg
     1441 cgccgccgcc tcatccgctc gctagagcat gttcaggtcc agctgtcgct ctcctacagc
     1501 cgccgcgggg acctggagat cttcctcacc agccccatgg gcacgcgctc cacgcttgtg
     1561 gccatcagac ccttggatat cagcggccaa ggctacaaca actggatctt catgtctact
     1621 cactactggg atgaggaccc gcagggcctg tggaccctgg ggctggagaa taagggctac
     1681 tattataaca caggaactct gtactactgc acgctgctgc tgtatgggac ggcagaggac
     1741 atgacagcgc ggccccagac cccccaggtg accagctgcg cgcacgcatg tgcagaggga
     1801 cacagagggg ctgtgccagg aaagtcattg tcccctctcc attgtggcag aactctgcct
     1861 catctccagc aagcagtggt ggtggctcta cagccacaca cagcagccag tgaccaaggg
     1921 acaggacagc tgtcaccctc ctaccacacc tgctcggcag cttgaccagc gactacactg
     1981 cctgttccct gcccctcatg ctgggagtgc ttcagagccc ctccaaggct tgtcacctct
     2041 ggcagccatc ctggctatca gtcttgggcc atggtgctgt ccctgctaac cagggccttt
     2101 ggaaggcccc tcatcttgag gaaggcccac ctctccccag gctggatacc cctggggagc
     2161 cagagatgcc ccactctcag gacagaaggc cggtacccca aggccctgct cccaggccgg
     2221 gataggaata tgccccagaa ggccacggca gagagctgca tgggtcacgt gacagcccgc
     2281 agctcagcct cagctgctcc cagtggaaga gacgtttcct cattcttttt ggagcaggta
     2341 tggcagacaa gaggtcagac cacagccacc aaccacctgc cccttccctg tctccaaacc
     2401 atccccatgt ctaacctcat agttggcaaa taaagttaaa cagaaaaaaa aaaaaaaa
//
