LOCUS       NM_012746               2446 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Rattus norvegicus proprotein convertase subtilisin/kexin type 2
            (Pcsk2), mRNA.
ACCESSION   NM_012746
VERSION     NM_012746.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Winters,A., Ramos-Molina,B., Jarvela,T.S., Yerges-Armstrong,L.,
            Pollin,T.I. and Lindberg,I.
  TITLE     Functional analysis of PCSK2 coding variants: A founder effect in
            the Old Order Amish population
  JOURNAL   Diabetes Res Clin Pract 131, 82-90 (2017)
   PUBMED   28719828
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Maddila,S.C., Busch-Dienstfertig,M. and Stein,C.
  TITLE     B Lymphocytes Express Pomc mRNA, Processing Enzymes and
            beta-Endorphin in Painful Inflammation
  JOURNAL   J Neuroimmune Pharmacol 12 (1), 180-186 (2017)
   PUBMED   27837406
REFERENCE   3  (bases 1 to 2446)
  AUTHORS   Toorie,A.M., Cyr,N.E., Steger,J.S., Beckman,R., Farah,G. and
            Nillni,E.A.
  TITLE     The Nutrient and Energy Sensor Sirt1 Regulates the
            Hypothalamic-Pituitary-Adrenal (HPA) Axis by Altering the
            Production of the Prohormone Convertase 2 (PC2) Essential in the
            Maturation of Corticotropin-releasing Hormone (CRH) from Its
            Prohormone in Male Rats
  JOURNAL   J Biol Chem 291 (11), 5844-5859 (2016)
   PUBMED   26755731
  REMARK    GeneRIF: The nutrient sensor sirtuin 1 regulates the production of
            CRH post-translationally by affecting PC2 post-translationally by
            affecting PC2.
REFERENCE   4  (bases 1 to 2446)
  AUTHORS   Fujimaki,T., Kato,K., Yokoi,K., Oguri,M., Yoshida,T., Watanabe,S.,
            Metoki,N., Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y., Kimura,G.
            and Yamada,Y.
  TITLE     Association of genetic variants in SEMA3F, CLEC16A, LAMA3, and
            PCSK2 with myocardial infarction in Japanese individuals
  JOURNAL   Atherosclerosis 210 (2), 468-473 (2010)
   PUBMED   20036365
REFERENCE   5  (bases 1 to 2446)
  AUTHORS   Takahashi,T., Ida,T., Sato,T., Nakashima,Y., Nakamura,Y., Tsuji,A.
            and Kojima,M.
  TITLE     Production of n-octanoyl-modified ghrelin in cultured cells
            requires prohormone processing protease and ghrelin
            O-acyltransferase, as well as n-octanoic acid
  JOURNAL   J Biochem 146 (5), 675-682 (2009)
   PUBMED   19628676
  REMARK    GeneRIF: The presence of PC and GOAT in the cells, as well as
            n-octanoic acid in the culture medium, was necessary to produce
            n-octanoyl ghrelin.
REFERENCE   6  (bases 1 to 2446)
  AUTHORS   Bailyes,E.M., Shennan,K.I., Usac,E.F., Arden,S.D., Guest,P.C.,
            Docherty,K. and Hutton,J.C.
  TITLE     Differences between the catalytic properties of recombinant human
            PC2 and endogenous rat PC2
  JOURNAL   Biochem J 309 (Pt 2) (Pt 2), 587-594 (1995)
   PUBMED   7626024
REFERENCE   7  (bases 1 to 2446)
  AUTHORS   Rhodes,C.J., Lincoln,B. and Shoelson,S.E.
  TITLE     Preferential cleavage of des-31,32-proinsulin over intact
            proinsulin by the insulin secretory granule type II endopeptidase.
            Implication of a favored route for prohormone processing
  JOURNAL   J Biol Chem 267 (32), 22719-22727 (1992)
   PUBMED   1429623
REFERENCE   8  (bases 1 to 2446)
  AUTHORS   Hakes,D.J., Birch,N.P., Mezey,A. and Dixon,J.E.
  TITLE     Isolation of two complementary deoxyribonucleic acid clones from a
            rat insulinoma cell line based on similarities to Kex2 and furin
            sequences and the specific localization of each transcript to
            endocrine and neuroendocrine tissues in rats
  JOURNAL   Endocrinology 129 (6), 3053-3063 (1991)
   PUBMED   1954888
REFERENCE   9  (bases 1 to 2446)
  AUTHORS   Bloomquist,B.T., Eipper,B.A. and Mains,R.E.
  TITLE     Prohormone-converting enzymes: regulation and evaluation of
            function using antisense RNA
  JOURNAL   Mol Endocrinol 5 (12), 2014-2024 (1991)
   PUBMED   1791845
REFERENCE   10 (bases 1 to 2446)
  AUTHORS   Davidson,H.W., Rhodes,C.J. and Hutton,J.C.
  TITLE     Intraorganellar calcium and pH control proinsulin cleavage in the
            pancreatic beta cell via two distinct site-specific endopeptidases
  JOURNAL   Nature 333 (6168), 93-96 (1988)
   PUBMED   3283564
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 26, 2020 this sequence version replaced NM_012746.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M76706.1, M83746.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-496               JAXUCZ010000003.1  151333938-151334433
            497-601             JAXUCZ010000003.1  151377890-151377994
            602-715             JAXUCZ010000003.1  151492416-151492529
            716-824             JAXUCZ010000003.1  151494972-151495080
            825-862             JAXUCZ010000003.1  151500397-151500434
            863-939             JAXUCZ010000003.1  151549993-151550069
            940-1028            JAXUCZ010000003.1  151595252-151595340
            1029-1204           JAXUCZ010000003.1  151605765-151605940
            1205-1420           JAXUCZ010000003.1  151615064-151615279
            1421-1521           JAXUCZ010000003.1  151617804-151617904
            1522-1749           JAXUCZ010000003.1  151622972-151623199
            1750-2446           JAXUCZ010000003.1  151635932-151636628
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q41"
     gene            1..2446
                     /gene="Pcsk2"
                     /note="proprotein convertase subtilisin/kexin type 2"
                     /db_xref="GeneID:25121"
                     /db_xref="RGD:3273"
     exon            1..496
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             323..2236
                     /gene="Pcsk2"
                     /EC_number="3.4.21.94"
                     /note="neuroendocrine convertase 2; PC2; NEC 2; prohormone
                     convertase 2; proprotein convertase 2; KEX2-like
                     endoprotease 2"
                     /codon_start=1
                     /product="neuroendocrine convertase 2 precursor"
                     /protein_id="NP_036878.1"
                     /db_xref="GeneID:25121"
                     /db_xref="RGD:3273"
                     /translation="MEGGCGSQWKAAGLLFCVMVFASAERPVFTNHFLVELHKDGEEE
                     ARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKRQLERDPRIKMALQQEG
                     FDRKKRGYRDINEIDINMNDPLFTKQWYLFNTGQADGTPGLDLNVAEAWELGYTGKGV
                     TIGIMDDGIDYLHPDLAYNYNSDASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAA
                     ASNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKT
                     VDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAI
                     NDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGV
                     FALALEANVDLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGA
                     MVKMAKDWKTVPERFHCVGGSVQNPEKIPPTGKLVLTLQTNACEGKENFVRYLEHVQA
                     VITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGT
                     WTLELGFVGSAPQKGLLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKQELEEELDE
                     AVERSLQSILRKN"
     exon            497..601
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            602..715
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            716..824
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..862
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            863..939
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1028
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1204
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1420
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1521
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1749
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..2446
                     /gene="Pcsk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaaaatgcag atttagcatc aagcacagac acacactagc tctttctctc gggtacacac
       61 agctccgcac attcgcaccc ctgccagtca gccgggctgc gcgactccac ggcttccctg
      121 gcagccagct gctggtggac agcgcacaga tttgctgttt gccaagcttc tcttctgcct
      181 cttttgctac acaaagattt ttttaaaaaa gaaaaacaga aacaaaaact acatacctat
      241 tttttatttg catcttccct cttcttcccc tgctccacca ccctgcgcgc ctcgcagccc
      301 cacttttcac tcccaaagaa ggatggaggg cggttgtgga tctcagtgga aggcggccgg
      361 gcttctcttc tgtgtcatgg tttttgcgtc tgccgagaga ccggtcttca cgaatcattt
      421 tcttgtggag ttgcataaag acggagagga agaggctcgc caagttgcag cagaacacgg
      481 atttggagtc cgaaagctcc cctttgcaga aggcctgtac cacttttacc acaatggcct
      541 tgcgaaggcc aaaagaagac gcagcctaca tcacaagcgg cagctggaga gagaccccag
      601 gataaagatg gccctgcagc aagaaggatt tgaccgtaaa aagagagggt acagagacat
      661 caatgagatt gacatcaata tgaatgatcc tctttttaca aagcaatggt acctgttcaa
      721 cactgggcaa gctgacggga ctcctgggct agacttgaat gtggccgaag cctgggagct
      781 gggatacaca gggaaaggag tgaccattgg aatcatggac gatggaattg actatctcca
      841 cccagacctg gcctacaact ataactctga tgcaagttat gacttcagca gcaatgaccc
      901 ctatccatac cctcgataca cagatgactg gttcaacagc catggaacca ggtgtgcagg
      961 agaagtttcc gctgcagcca gcaacaatat ctgtggagtc ggtgtagcat acaactccaa
     1021 ggtggcaggt atccggatgc tggaccagcc ctttatgact gacatcatcg aggcctcctc
     1081 catcagccac atgcctcaac tgatcgacat ctacagtgca agctggggcc ccacagacaa
     1141 cgggaagaca gttgatgggc cccgagagct cacgctccag gccatggctg atggtgtgaa
     1201 caagggccgt gggggcaaag gcagcatcta tgtgtgggcc tccggggacg gtggcagcta
     1261 tgatgactgc aactgtgacg gctatgcgtc aagcatgtgg accatctcca tcaactcggc
     1321 catcaacgac ggcaggaccg ccttgtatga cgagagctgc tcttccacct tggcctctac
     1381 gttcagcaac gggaggaaga ggaatcccga ggccggtgtg gctaccacag acttgtatgg
     1441 caactgtact ctgaggcatt ctgggacatc tgcagctgct cctgaggcag ctggcgtgtt
     1501 tgcattggct ttggaggcta acgtggatct gacctggcga gacatgcaac atctgacagt
     1561 gctcacctcc aagcggaacc agcttcacga tgaggttcat cagtggcgac ggaatggggt
     1621 tggcctggaa ttcaatcacc tctttggcta cggagtcctt gatgcaggtg ccatggtgaa
     1681 aatggctaaa gactggaaaa ctgtcccaga gagattccac tgtgtgggag gctcggtgca
     1741 gaaccctgag aaaataccac ccaccggcaa gttggtactg accctccaaa caaatgcatg
     1801 tgaggggaaa gaaaacttcg tccgctacct cgagcatgtc caagctgtca tcacagtcaa
     1861 cgcaaccagg agaggagacc tgaacatcaa catgacctcc ccgatgggca ccaagtccat
     1921 tttgctaagc cggcgtccaa gagacgacga ctccaaggtg ggctttgaca agtggccttt
     1981 catgaccacc cacacctggg gggaggatgc ccgagggacc tggaccctgg agctggggtt
     2041 tgtgggcagt gcaccacaga agggcttgct gaaggaatgg accctcatgc tacatggcac
     2101 ccagagtgcc ccatacatcg atcaggtggt gagggattac caatcgaagt tggccatgtc
     2161 caagaagcaa gagctggagg aagagctgga tgaagctgtg gagagaagtc tgcaaagtat
     2221 cctgagaaag aactagggcc acgcttccgc ctccatctcc ccttcctccc tgtctctgcc
     2281 tctccttggt ccacagttct ggcagccacc agccacccag caattcctgt taccctcaca
     2341 caagcagtcc cagcctggtc tgaagctttg cttgctctca atgattattt tcactacaat
     2401 ggaagcaatc atttttattc tgtagcccaa atatagcgtt cctacc
//
