LOCUS       NM_017091               5046 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Rattus norvegicus proprotein convertase subtilisin/kexin type 1
            (Pcsk1), mRNA.
ACCESSION   NM_017091
VERSION     NM_017091.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Maddila,S.C., Busch-Dienstfertig,M. and Stein,C.
  TITLE     B Lymphocytes Express Pomc mRNA, Processing Enzymes and
            beta-Endorphin in Painful Inflammation
  JOURNAL   J Neuroimmune Pharmacol 12 (1), 180-186 (2017)
   PUBMED   27837406
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Duhamel,M., Rodet,F., Murgoci,A.N., Desjardins,R., Gagnon,H.,
            Wisztorski,M., Fournier,I., Day,R. and Salzet,M.
  TITLE     The proprotein convertase PC1/3 regulates TLR9 trafficking and the
            associated signaling pathways
  JOURNAL   Sci Rep 6, 19360 (2016)
   PUBMED   26778167
  REMARK    GeneRIF: macrophages from PC1/3 KO mice and rat PC1/3-KD NR8383
            macrophages secreted more pro-inflammatory cytokines such as
            TNF-alpha, IL6, IL1alpha and CXCL2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5046)
  AUTHORS   Gagnon,H., Refaie,S., Gagnon,S., Desjardins,R., Salzet,M. and
            Day,R.
  TITLE     Proprotein convertase 1/3 (PC1/3) in the rat alveolar macrophage
            cell line NR8383: localization, trafficking and effects on cytokine
            secretion
  JOURNAL   PLoS One 8 (4), e61557 (2013)
   PUBMED   23637853
  REMARK    GeneRIF: PC1/3 as an important regulator of vesicle trafficking and
            secretion in macrophages.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5046)
  AUTHORS   Navarro,M., Cubero,I. and Thiele,T.E.
  TITLE     Decreased immunoreactivity of the polypeptide precursor
            pro-opiomelanocortin (POMC) and the prohormone convertase pc1/3
            after chronic ethanol exposure in Sprague-Dawley rats
  JOURNAL   Alcohol Clin Exp Res 37 (3), 399-406 (2013)
   PUBMED   23050949
  REMARK    GeneRIF: Chronic ethanol exposure decreases prohormone convertase
            (pc1/3) immunoreactivity in brain.
REFERENCE   5  (bases 1 to 5046)
  AUTHORS   Takahashi,T., Ida,T., Sato,T., Nakashima,Y., Nakamura,Y., Tsuji,A.
            and Kojima,M.
  TITLE     Production of n-octanoyl-modified ghrelin in cultured cells
            requires prohormone processing protease and ghrelin
            O-acyltransferase, as well as n-octanoic acid
  JOURNAL   J Biochem 146 (5), 675-682 (2009)
   PUBMED   19628676
  REMARK    GeneRIF: The presence of PC and GOAT in the cells, as well as
            n-octanoic acid in the culture medium, was necessary to produce
            n-octanoyl ghrelin.
REFERENCE   6  (bases 1 to 5046)
  AUTHORS   Malide,D., Seidah,N.G., Chretien,M. and Bendayan,M.
  TITLE     Electron microscopic immunocytochemical evidence for the
            involvement of the convertases PC1 and PC2 in the processing of
            proinsulin in pancreatic beta-cells
  JOURNAL   J Histochem Cytochem 43 (1), 11-19 (1995)
   PUBMED   7822759
REFERENCE   7  (bases 1 to 5046)
  AUTHORS   Rhodes,C.J., Lincoln,B. and Shoelson,S.E.
  TITLE     Preferential cleavage of des-31,32-proinsulin over intact
            proinsulin by the insulin secretory granule type II endopeptidase.
            Implication of a favored route for prohormone processing
  JOURNAL   J Biol Chem 267 (32), 22719-22727 (1992)
   PUBMED   1429623
REFERENCE   8  (bases 1 to 5046)
  AUTHORS   Hakes,D.J., Birch,N.P., Mezey,A. and Dixon,J.E.
  TITLE     Isolation of two complementary deoxyribonucleic acid clones from a
            rat insulinoma cell line based on similarities to Kex2 and furin
            sequences and the specific localization of each transcript to
            endocrine and neuroendocrine tissues in rats
  JOURNAL   Endocrinology 129 (6), 3053-3063 (1991)
   PUBMED   1954888
REFERENCE   9  (bases 1 to 5046)
  AUTHORS   Bloomquist,B.T., Eipper,B.A. and Mains,R.E.
  TITLE     Prohormone-converting enzymes: regulation and evaluation of
            function using antisense RNA
  JOURNAL   Mol Endocrinol 5 (12), 2014-2024 (1991)
   PUBMED   1791845
REFERENCE   10 (bases 1 to 5046)
  AUTHORS   Davidson,H.W., Rhodes,C.J. and Hutton,J.C.
  TITLE     Intraorganellar calcium and pH control proinsulin cleavage in the
            pancreatic beta cell via two distinct site-specific endopeptidases
  JOURNAL   Nature 333 (6168), 93-96 (1988)
   PUBMED   3283564
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            M76705.1, M83745.1 and JAXUCZ010000002.1.
            
            On Oct 14, 2005 this sequence version replaced NM_017091.1.
            
            Sequence Note: This RefSeq record was created using sequences from
            two different strains, Sprague-Dawley and BN/SsNHsdMCW, because no
            single transcript from the same strain was available for the full
            length of the gene.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M83745.1, M76705.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                M76705.1           2-57
            57-1314             M83745.1           1-1258
            1315-2657           M76705.1           1317-2659
            2658-5046           JAXUCZ010000002.1  6174586-6176974
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q11"
     gene            1..5046
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /note="proprotein convertase subtilisin/kexin type 1"
                     /db_xref="GeneID:25204"
                     /db_xref="RGD:3272"
     exon            1..399
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..2478
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /EC_number="3.4.21.93"
                     /note="prohormone convertase 3; neuroendocrine convertase
                     1; NEC 1; prohormone convertase 1; proprotein convertase
                     1; Protein convertase subtilisin / kexin, type I"
                     /codon_start=1
                     /product="neuroendocrine convertase 1 precursor"
                     /protein_id="NP_058787.1"
                     /db_xref="GeneID:25204"
                     /db_xref="RGD:3272"
                     /translation="MKQRGWTLQCTAFTLFCVWCALNSVKAKRQFVNEWAAEIHGGPE
                     AASAIAEELGYDLLGQIGSLENHYLFKHKNHPRRSRRSALHITKRLSDDDRVIWAEQQ
                     YEKERRKRSVPRDSALNLFNDPMWNQQWYLQDTRMTASLPKLDLHVIPVWQKGITGKG
                     VVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAM
                     QANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKT
                     VEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSA
                     SQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIF
                     ALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALV
                     DLADPRTWRNVPEKKECIIKDNNFEPRALKANGEVIVEIPTRACEGQENAINSLEHVQ
                     FEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPVGT
                     WTLKVTDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMV
                     NVVEEKPTQNSLNGNLLVPKNSSSSSVEDRRDEQVQGAPSKAMLRLLQSAFSKNTPSK
                     QSSKIPSAKLSVPYEGLYEALEKLNKPSQLEDSEDSLYSDYVDVFYNTKPYKHRDDRL
                     LQALMDILNEKN"
     exon            400..504
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            505..615
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            616..762
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            763..839
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            840..928
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1101
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1314
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1415
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1649
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1807
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1941
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..2103
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..5046
                     /gene="Pcsk1"
                     /gene_synonym="BDP; PC1; PC3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttctattctg gctgggaagg ctggggctcc tctcagccag gagactgaag tgcttcagtg
       61 agcgctggct catcgccgcc cagcctctcc cagggagcct cctagctcta gtaaagcaac
      121 ccagagccag gagcagtgtg cagggtggaa aggtctagcc tagctggtgt gtttctgatc
      181 ttgcttcttt tctctcagcc cttcctactt gtgcgagcca tgaagcaaag aggttggact
      241 ctgcagtgta ctgctttcac cctcttttgc gtttggtgtg cactgaacag tgtaaaagcg
      301 aagaggcagt ttgtcaatga atgggcggcg gagatacatg gagggccaga agctgcttct
      361 gccattgccg aagagctggg ctatgacctc ttgggtcaga ttggatcact tgaaaatcac
      421 tatttattca aacacaaaaa tcatcctcga agatcccgaa gaagtgctct tcatatcact
      481 aagaggttat ctgatgatga tcgtgtgata tgggcggaac aacaatatga aaaagagaga
      541 agaaaacgtt cagttccaag agactcagca ttgaatctct tcaatgatcc aatgtggaat
      601 cagcagtggt acttgcaaga caccagaatg actgcatctc tgcccaagct ggacttgcat
      661 gtaatacctg tttggcaaaa gggtattact ggcaaaggag ttgttattac tgtactggac
      721 gatggcttgg agtggaatca cacagatatt tatgccaatt atgacccaga ggctagctat
      781 gattttaatg ataatgatca tgatccattt ccccgatatg atcccacaaa tgaaaacaaa
      841 catggaacaa gatgtgcagg agaaattgcc atgcaagcaa ataatcacaa atgtggagtt
      901 ggagttgcat ataattccaa agttggaggc ataagaatgc tggatggcat tgtaactgat
      961 gccattgagg ctagttcaat tggattcaac cctggccatg tggatattta cagtgcaagc
     1021 tggggcccta atgatgatgg aaaaactgtg gaggggcctg gcagactagc ccagaaggca
     1081 tttgaatacg gtgtcaaaca ggggagacaa ggaaaaggct ccatttttgt ctgggcttca
     1141 gggaatgggg gtcgtcaagg agataactgt gactgtgatg gctacacaga cagcatttac
     1201 accatctcta tcagcagtgc ctcccagcag ggcctgtcac cctggtatgc agagaagtgt
     1261 tcctccacac tggccacctc ctacagcagt ggtgattaca cagaccaacg aataactagt
     1321 gcggacctgc acaatgactg cacagagacc cacacaggca cctcagcttc tgcacctctg
     1381 gctgctggca tctttgctct agccttggaa gcaaacccaa atctcacctg gcgagatatg
     1441 cagcatctgg ttgtctggac ctctgagtat gacccattgg ccaataaccc aggttggaaa
     1501 aagaatggag cgggcttgat ggtgaacagc cgatttggat ttggcttgct aaatgccaaa
     1561 gctctggtgg atttggctga tcctagaact tggagaaacg tgcctgagaa gaaagaatgt
     1621 atcataaaag acaacaactt tgagcctaga gccctgaaag ctaatggaga agtgattgtt
     1681 gaaatcccta caagagcttg tgaaggacaa gaaaatgcta tcaattccct ggaacatgtg
     1741 caatttgaag caacaatcga atattctcgt agaggagacc ttcatgtcac acttacctct
     1801 gctgctggaa ccagcaccgt actgttggct gaaagggaaa gggatacatc ccccaatggc
     1861 tttaagaatt gggacttcat gtctgttcat acatggggag agaatcctgt aggcacctgg
     1921 acattgaaag ttacagacat gtctggaaga atgcaaaatg aaggaaggat tgtgaactgg
     1981 aagttgattt tgcatggcac atcttctcag ccagaacaca tgaagcagcc tcgtgtatac
     2041 acgtcctaca atactgtcca gaatgacagg agaggagtgg aaaagatggt gaatgttgtg
     2101 gaggagaagc ccacacaaaa cagcctgaat ggcaatctcc tggtacccaa aaactccagc
     2161 agcagcagtg tggaagacag aagagatgag caggtacaag gagctccttc aaaggccatg
     2221 ctacggctcc tacaaagtgc tttcagcaag aacacacctt caaagcaatc atcaaagatt
     2281 ccaagtgcta agctcagtgt cccttatgag ggtctctatg aagccctgga aaagctcaac
     2341 aagccctccc agcttgaaga ctctgaagac agtctgtata gcgactatgt tgatgttttc
     2401 tataacacaa aaccttataa gcatagagat gacaggctgc tgcaagcact catggacatt
     2461 ctgaatgaga agaattaaaa taagtgtata tggcctgagt tagaaatcct tatgtaccct
     2521 ccttttcctt agatgttcct gggtctggag ttgttgtatc cctgcttcct gtgattataa
     2581 tgtccttcat tgtaatttta ctttttcttc tcaaactgta cttttggagt gagttccaaa
     2641 atgaaagcca acttcataat tggtctcagt gtttaagaat atgtcttccc tgtctataca
     2701 agtgaagcaa cttctttcta aagtcgctgt tgggacacaa ccctttgctg cagtagaaca
     2761 gctcctaaag cctccccatt tcatatctca caagaatagg aaaagagtgg gagatttaga
     2821 aaagtaatca acaatggtag tttgagggct acagcttatc ttgtttccta tttttgcaat
     2881 ggggttcttt gaattttgat aacaaggaag catttataaa gtatctagtg agaaagttca
     2941 caactaggtt cagatcataa gtaacatgta ttactaatgc tgagactgat aaactgacca
     3001 ttttggagga gctggaataa aatgctttat ttaaattcag aaacagagct taagattttt
     3061 attagctaga atagttgctc tcaattggtg taccatgtag aggtctctgt gggtcgaaag
     3121 ttggctgtga cccttgctgt atgagacctg gctttctatt tccccctttg tgccttgtat
     3181 gtttcttttt tgtgtggcaa actcaaaatt caaagaaaga actaattcat gtgattttct
     3241 caaaacaaag ttatacttca ttttggcaaa ttcctagtca taattttgtt taataacaag
     3301 gagtcactca aaaatataga aagccaatat aataacaggg aaaatgttca caaagcagag
     3361 aagcagcaag actccctttg caagctgtat tcctaggaca gcttcttttg gttctctata
     3421 tgaatggtca tttctggaca ccacaggtct tctgtagtct cagagaacag tattcaaatt
     3481 cttaacaaaa tgcttccatg tttcaaatca aaaacaggta gacaacactt aggtcttatt
     3541 tttattctct gtaagttatg aagaatattt cagaagtgtg aaagactgat gaaagtgtga
     3601 agtgtagatc tcatataaaa cccgattctc ggacccatct tccatcacag caacagctag
     3661 ctagagatag caacttccaa gtaaaattta aagtcaagcc ttccctggtc catatatttt
     3721 tttgttttac aatagccagt taattggtta ctactcagca tccaagccat ttattccttt
     3781 ttaattatca tctattagtt gcagggagtc agatttcatt ttgacattct catacacgta
     3841 tttcatgatt tgtacaatgt ccatcccgca tcagcatctc ttctgtctct ccttgcttgt
     3901 cctcttgctc ttctaagtat tcccacttcc actttcagga ctttttatct aactcagatt
     3961 ccacatataa gagaaaatgt gatgtttgtc tttctatgca tgtaactttg cctactcata
     4021 tactaagtga atctatatcg gtgtaaatac attaggaaaa gtatttcctg caattatttc
     4081 caagcagtct ttgccgttaa taattgaatt tgatatgagc tagttaaatt gttgcattta
     4141 taagtaaaac agtgagtact tcggacccac aaaatacctc taaagtctcc tttaatatga
     4201 acacttgatc ttatctcacc tggatgttca aatgtactat tcattaacta gagatttcct
     4261 tctcttaata tgccaacata cactctagtt aactctctgc ctgtgttgtc caactgagtt
     4321 tctgtttgca aatgtcctac acagtctaca tccactgcta agacagctaa tttgtctttg
     4381 ctttgagtga gattagtagc acaaattttc acctcctgtt tcatcatttg catttgtaca
     4441 taacaaagcc ctttccagag ccttgccctt gatccctctg attttgaagg ttttgcaatg
     4501 tgtttactat aaggtttaaa taaaatgttt actattatag cacattacaa ctgaaaagga
     4561 cttaagagtt aaaacattaa atttcagcta cttagaaaag acctaaagaa taatatcagt
     4621 aactgacaat taatatgtga ttttaaaaaa cgaaagagtg ggaggtcaac cccacccttt
     4681 ctggtcctta ttctaactta tggagatttt tcttcagcac tttctaatat atatttcccc
     4741 tctaagaacc ctaaccaatt catgggctat agccaaatat tcacttgggt aagctttaat
     4801 atttagacaa taccatgttt gaatgtttcc aacctggagt atatattgtc tacacataaa
     4861 aagaatattt ttgtcagcct ttcttagtgc ctatgtggat ttttgtgaaa atgttaaaaa
     4921 aggataatta tataatattt cccttggaat attaagctat attttcttta caagtatttg
     4981 taatgtacca atgcttttaa caacagaatt ttaaaaagca taataaatta tattaaagaa
     5041 tcaaaa
//
