LOCUS       NM_001270538             698 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript
            variant 2, mRNA.
ACCESSION   NM_001270538
VERSION     NM_001270538.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Xie,Y.Y., Sun,M.M., Lou,X.F., Zhang,C., Han,F., Zhang,B.Y., Wang,P.
            and Lu,Y.M.
  TITLE     Overexpression of PEP-19 suppresses angiotensin II-induced
            cardiomyocyte hypertrophy
  JOURNAL   J Pharmacol Sci 125 (3), 274-282 (2014)
   PUBMED   25048017
  REMARK    GeneRIF: Overexpression of PEP-19 suppresses angiotensin II-induced
            cardiomyocyte hypertrophy.
REFERENCE   2  (bases 1 to 698)
  AUTHORS   Wang,X., Xiong,L.W., El Ayadi,A., Boehning,D. and Putkey,J.A.
  TITLE     The calmodulin regulator protein, PEP-19, sensitizes ATP-induced
            Ca2+ release
  JOURNAL   J Biol Chem 288 (3), 2040-2048 (2013)
   PUBMED   23204517
REFERENCE   3  (bases 1 to 698)
  AUTHORS   Suzuki,T., Sato,T., Sasaki,R. and Ichikawa,H.
  TITLE     Peptide 19-containing neurons in the medullary dorsal horn,
            subnuclei interpolaris and oralis, and nucleus principalis of the
            rat
  JOURNAL   Ann Anat 194 (4), 321-328 (2012)
   PUBMED   22197496
  REMARK    GeneRIF: These findings suggest that PEP 19-ir neurons in the MDH
            may have a function in modulation of nociceptive and
            thermo-receptive signaling.
REFERENCE   4  (bases 1 to 698)
  AUTHORS   Morgan,M.A. and Morgan,J.I.
  TITLE     Pcp4l1 contains an auto-inhibitory element that prevents its IQ
            motif from binding to calmodulin
  JOURNAL   J Neurochem 121 (6), 843-851 (2012)
   PUBMED   22458599
REFERENCE   5  (bases 1 to 698)
  AUTHORS   Saladino,P., Di Liegro,C.M., Proia,P., Sala,A., Schiera,G., Lo
            Cicero,A. and Di Liegro,I.
  TITLE     RNA-binding activity of the rat calmodulin-binding PEP-19 protein
            and of the long PEP-19 isoform
  JOURNAL   Int J Mol Med 29 (2), 141-145 (2012)
   PUBMED   22020791
  REMARK    GeneRIF: PEP-19 and LPI bind H1 histone mRNA and that this binding
            is affected by calmodulin.
REFERENCE   6  (bases 1 to 698)
  AUTHORS   Johanson,R.A., Sarau,H.M., Foley,J.J. and Slemmon,J.R.
  TITLE     Calmodulin-binding peptide PEP-19 modulates activation of
            calmodulin kinase II In situ
  JOURNAL   J Neurosci 20 (8), 2860-2866 (2000)
   PUBMED   10751438
REFERENCE   7  (bases 1 to 698)
  AUTHORS   Ichikawa,H. and Sugimoto,T.
  TITLE     Peptide 19-immunoreactive primary sensory neurons in the rat
            trigeminal ganglion
  JOURNAL   Brain Res 846 (2), 274-279 (1999)
   PUBMED   10556647
REFERENCE   8  (bases 1 to 698)
  AUTHORS   Utal,A.K., Stopka,A.L., Roy,M. and Coleman,P.D.
  TITLE     PEP-19 immunohistochemistry defines the basal ganglia and
            associated structures in the adult human brain, and is dramatically
            reduced in Huntington's disease
  JOURNAL   Neuroscience 86 (4), 1055-1063 (1998)
   PUBMED   9697113
  REMARK    Erratum:[Neuroscience 1999;88(3):993]
REFERENCE   9  (bases 1 to 698)
  AUTHORS   Sangameswaran,L., Hempstead,J. and Morgan,J.I.
  TITLE     Molecular cloning of a neuron-specific transcript and its
            regulation during normal and aberrant cerebellar development
  JOURNAL   Proc Natl Acad Sci U S A 86 (14), 5651-5655 (1989)
   PUBMED   2748608
REFERENCE   10 (bases 1 to 698)
  AUTHORS   Ziai,R., Pan,Y.C., Hulmes,J.D., Sangameswaran,L. and Morgan,J.I.
  TITLE     Isolation, sequence, and developmental profile of a brain-specific
            polypeptide, PEP-19
  JOURNAL   Proc Natl Acad Sci U S A 83 (21), 8420-8423 (1986)
   PUBMED   3464961
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000011.1 and
            CB613293.1.
            
            On Dec 9, 2014 this sequence version replaced NM_001270538.1.
            
            Summary: The protein encoded by this gene regulates H1&#730; and
            H3.3 histone synthesis by binding to H1&#730; and H3.3 histone
            mRNAs. The encoded protein can bind calmodulin as well, providung a
            possible link between calcium-dependent signals and histone
            metabolism. Two transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (2) contains an alternate exon
            compared to variant 1, which causes a frameshift and use of a
            different start codon compared to variant 1. The resulting isoform
            (LPI) has a longer and distinct N-terminus compared to isoform
            PEP19. Experimental support for this transcript and isoform is
            provided in PMID:22020791 and PMID:17273800.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ493658.1, SRR26360176.1588824.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840129, SAMN14984027
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                JAXUCZ010000011.1  49270186-49270200
            16-127              CB613293.1         1-112
            128-254             JAXUCZ010000011.1  49329135-49329261
            255-698             CB613293.1         113-556
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q11"
     gene            1..698
                     /gene="Pcp4"
                     /gene_synonym="pep-19; PEPZ19"
                     /note="Purkinje cell protein 4"
                     /db_xref="GeneID:25510"
                     /db_xref="RGD:3271"
     exon            1..75
                     /gene="Pcp4"
                     /gene_synonym="pep-19; PEPZ19"
                     /inference="alignment:Splign:2.1.0"
     exon            76..127
                     /gene="Pcp4"
                     /gene_synonym="pep-19; PEPZ19"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..382
                     /gene="Pcp4"
                     /gene_synonym="pep-19; PEPZ19"
                     /note="isoform LPI is encoded by transcript variant 2;
                     brain-specific antigen PCP-4; brain-specific polypeptide
                     PEP-19; Neuron specific protein PEP-19 (Purkinje cell
                     protein 4); calmodulin regulator protein PCP4"
                     /codon_start=1
                     /product="calmodulin regulator protein PCP4 isoform LPI"
                     /protein_id="NP_001257467.1"
                     /db_xref="GeneID:25510"
                     /db_xref="RGD:3271"
                     /translation="MEKTRHQEIMAKCSLPEEEWRSAWHDPARTCSVSKKAKRICSGN
                     ASLEAPEIYGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQKKKAGSQS"
     exon            128..254
                     /gene="Pcp4"
                     /gene_synonym="pep-19; PEPZ19"
                     /inference="alignment:Splign:2.1.0"
     exon            255..698
                     /gene="Pcp4"
                     /gene_synonym="pep-19; PEPZ19"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcagccacc cctgagcatt gccttctgag ctgttctgtg ggaccaagct gcggagtcag
       61 gccaacatga gtgagagaca aagtgctgga gcgaccaatg gaaaagacaa gacatcagga
      121 gataatggca aagtgctcac tgccagagga ggaatggaga agtgcctggc acgatccagc
      181 cagaacctgc tcggtctcta agaaagccaa gaggatctgc agtggaaatg cctcgctgga
      241 ggctcctgaa atatatgggc agaagaaggt ccaagaagaa tttgatatcg acatggatgc
      301 accagagaca gagcgtgcag ctgtggccat tcagtctcag ttcagaaaat tccagaagaa
      361 aaaggcagga tcacagtcct agtggggaag ctgcttcctg gtccacccga agacaccaag
      421 ttcaaccacc gtccatccag aaatgagaag aacaataccc tagagcaaag tcatccacac
      481 ccagtacaca ctccgctgct aacctgaaat gcatgaacag aaacccatag tatttatgcc
      541 cctctaggca ggtgtccaca ataaaattgt gagcagctta atctgtctgt ctccattaca
      601 attcctctgc aacgattttc ctcgatgttg taataaaaag gaggtaagag gagccaatct
      661 agtgtctgcc ttcatttctc acattaggcg ggaccata
//
