LOCUS       NM_001398561            2715 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus protein-L-isoaspartate (D-aspartate)
            O-methyltransferase 1 (Pcmt1), transcript variant 1, mRNA.
ACCESSION   NM_001398561 NM_001398563
VERSION     NM_001398561.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Dung,T.T., Yi,Y.S., Heo,J., Yang,W.S., Kim,J.H., Kim,H.G.,
            Park,J.G., Yoo,B.C., Cho,J.Y. and Hong,S.
  TITLE     Critical role of protein L-isoaspartyl methyltransferase in basic
            fibroblast growth factor-mediated neuronal cell differentiation
  JOURNAL   BMB Rep 49 (8), 437-442 (2016)
   PUBMED   26973341
  REMARK    GeneRIF: PIMT was critical for bFGF-mediated neuronal
            differentiation of PC12 cells and regulated the MAPK and Akt
            signaling pathways.
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2715)
  AUTHORS   Chiasserini,D., van Weering,J.R., Piersma,S.R., Pham,T.V.,
            Malekzadeh,A., Teunissen,C.E., de Wit,H. and Jimenez,C.R.
  TITLE     Proteomic analysis of cerebrospinal fluid extracellular vesicles: a
            comprehensive dataset
  JOURNAL   J Proteomics 106, 191-204 (2014)
   PUBMED   24769233
REFERENCE   4  (bases 1 to 2715)
  AUTHORS   Kapadia,B., Viswakarma,N., Parsa,K.V., Kain,V., Behera,S.,
            Suraj,S.K., Babu,P.P., Kar,A., Panda,S., Zhu,Y.J., Jia,Y.,
            Thimmapaya,B., Reddy,J.K. and Misra,P.
  TITLE     ERK2-mediated phosphorylation of transcriptional coactivator
            binding protein PIMT/NCoA6IP at Ser298 augments hepatic
            gluconeogenesis
  JOURNAL   PLoS One 8 (12), e83787 (2013)
   PUBMED   24358311
  REMARK    GeneRIF: ERK2-mediated phosphorylation of transcriptional
            coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic
            gluconeogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2715)
  AUTHORS   Yan,G., Qin,Q., Yi,B., Chuprun,K., Sun,H., Huang,S. and Sun,J.
  TITLE     Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects
            cardiomyocytes against hypoxia induced apoptosis through inhibiting
            proapoptotic kinase Mst1
  JOURNAL   Int J Cardiol 168 (4), 3291-3299 (2013)
   PUBMED   23647599
REFERENCE   6  (bases 1 to 2715)
  AUTHORS   Boivin,D., Bilodeau,D. and Beliveau,R.
  TITLE     Immunochemical characterization of L-isoaspartyl-protein carboxyl
            methyltransferase from mammalian tissues
  JOURNAL   Biochem J 309 (Pt 3) (Pt 3), 993-998 (1995)
   PUBMED   7639720
REFERENCE   7  (bases 1 to 2715)
  AUTHORS   Shirasawa,T., Endoh,R., Zeng,Y.X., Sakamoto,K. and Mori,H.
  TITLE     Protein L-isoaspartyl methyltransferase: developmentally regulated
            gene expression and protein localization in the central nervous
            system of aged rat
  JOURNAL   Neurosci Lett 188 (1), 37-40 (1995)
   PUBMED   7783974
REFERENCE   8  (bases 1 to 2715)
  AUTHORS   Mizobuchi,M., Murao,K., Takeda,R. and Kakimoto,Y.
  TITLE     Tissue-specific expression of isoaspartyl protein carboxyl
            methyltransferase gene in rat brain and testis
  JOURNAL   J Neurochem 62 (1), 322-328 (1994)
   PUBMED   8263531
REFERENCE   9  (bases 1 to 2715)
  AUTHORS   Cassidy,E.M., Wakim,B.T., Ferguson,A.G. and Samarel,A.M.
  TITLE     Asparaginyl deamidation-methylation of rat ventricular myosin light
            chains
  JOURNAL   J Mol Cell Cardiol 23 (5), 589-601 (1991)
   PUBMED   1886138
REFERENCE   10 (bases 1 to 2715)
  AUTHORS   Sato,M., Yoshida,T. and Tuboi,S.
  TITLE     Primary structure of rat brain protein carboxyl methyltransferase
            deduced from cDNA sequence
  JOURNAL   Biochem Biophys Res Commun 161 (1), 342-347 (1989)
   PUBMED   2730663
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Dec 23, 2021 this sequence version replaced NM_001398563.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22399489.3078733.1,
                                           SRR23984936.507049.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-522               JAXUCZ010000001.1  3964327-3964848     c
            523-627             JAXUCZ010000001.1  3948081-3948185     c
            628-659             JAXUCZ010000001.1  3946656-3946687     c
            660-764             JAXUCZ010000001.1  3943589-3943693     c
            765-885             JAXUCZ010000001.1  3940521-3940641     c
            886-971             JAXUCZ010000001.1  3939848-3939933     c
            972-1141            JAXUCZ010000001.1  3938064-3938233     c
            1142-2715           JAXUCZ010000001.1  3932161-3933734     c
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1p13"
     gene            1..2715
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /note="protein-L-isoaspartate (D-aspartate)
                     O-methyltransferase 1"
                     /db_xref="GeneID:25604"
                     /db_xref="RGD:3268"
     exon            1..522
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1154
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /EC_number="2.1.1.77"
                     /note="isoform a is encoded by transcript variant 1;
                     protein-L-isoaspartate(D-aspartate) O-methyltransferase;
                     PIMT; protein-beta-aspartate methyltransferase;
                     L-isoaspartyl protein carboxyl methyltransferase; protein
                     L-isoaspartyl/D-aspartyl methyltransferase"
                     /codon_start=1
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase isoform a"
                     /protein_id="NP_001385490.1"
                     /db_xref="GeneID:25604"
                     /db_xref="RGD:3268"
                     /translation="MPGARSGGSGSDGSNSGRSSGDTSGAVTVWEVVSLLGKLLGTVA
                     ALKVVLYLLRVCFAMAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKSN
                     PYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGH
                     SGKVIGIDHIKELVDDSITNVKKDDPMLLSSGRVRLVVGDGRMGFAEEAPYDAIHVGA
                     AAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTD
                     KEKQWSRDEL"
     exon            523..627
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     exon            628..659
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     exon            660..764
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     exon            765..885
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     exon            886..971
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1141
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..2715
                     /gene="Pcmt1"
                     /gene_synonym="PCM"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccgaggagg cggcacctgt cggacccgcc atcccgccgc cgcgcatcag ccggagctgc
       61 ggctagaaac gagtcccacc gcgcagagta ccagttcttc agccgcagga cggaggcagg
      121 tgcaggcctc agcaagggga actcagcggc gttgatttgt ctgacctcgc tacggtcacc
      181 tcactggctg tcacctccac cagctacagg gcttcaggac tcccccgcct cgggccccgc
      241 ccggccgcac ccagggctgc gcggcgtcac agagcgcatg cgtgccgcgg gggatgccgg
      301 gagcgcgcag tggcggcagc ggcagcgacg gcagtaacag cggccgctcc agcggggaca
      361 ccagcggggc ggtgaccgtg tgggaggtgg tctcactctt ggggaaattg ctggggaccg
      421 tcgccgcgct gaaggtggtt ctatacctgc tccgggtgtg cttcgcgatg gcctggaaat
      481 ccggcggcgc cagccactcg gagctaatcc acaacctccg caagaatgga atcatcaaga
      541 cagataaagt atttgaggtg atgctggcta cagaccgctc ccactatgca aaatctaacc
      601 cttacatgga ttctccacag tcaataggtt tccaggcaac aatcagcgct cctcacatgc
      661 atgcatatgc actagagctc ctgtttgacc agctgcacga aggcgctaaa gcccttgacg
      721 tagggtctgg aagtggaatc ctcaccgcgt gttttgcacg gatggttgga cacagtggga
      781 aagtcattgg aattgatcac attaaagaac tagtggatga ctcaataact aatgtcaaaa
      841 aggatgaccc catgctcctg tcctctgggc gagtacggct ggttgtgggc gatggaagaa
      901 tggggtttgc tgaagaagcc ccttacgatg ccattcacgt cggagctgca gccccagttg
      961 tgccccaggc attaatagac cagttaaagc ctggtggaag attgatattg ccagtcggtc
     1021 ctgcaggagg aaaccaaatg ttggagcagt atgacaagct acaagatggc agtgtcaaaa
     1081 tgaagcctct gatgggggtg atatacgtgc ctttaacaga taaagaaaag cagtggtcca
     1141 gggacgagct gtgagagcaa cctgagcttg gccgtctgta acactgcagt ggattgctca
     1201 tctcggtcct caaagcattg agagccaaca gcatcaccgc ttgttttgga ctttgttaca
     1261 ctcttatttt cagcatgaaa atgtgtggtt tttgtagggt ttcctaattc aaaacggtgc
     1321 agagccagac tgatagagga gggctggaga gtgttaactt tgtgttgttt aacatgccca
     1381 catttcactt tacaaatatt aaaagaaagg gttctctaac cggaaagctt cgtttgtgac
     1441 ttagtttgga agagtggctt tgcttttctt ggacacttac ttctgttcca atattaattt
     1501 atgagatttc ttttgtgtgt cagataacac cacattcaca agtttagctt tcttggtagc
     1561 aatgtctgtt aaatgctact aagaaaatag agatgagctt tcttcttaaa gcctttgatg
     1621 tggcgtcata gaatagcatg ttatagatac catcagctgc tgacacctta aaactaggtt
     1681 tgtaaatact gaaacttaag acgatggcag gtagtgtccc aaaaacactc agctgttcag
     1741 atcggacatc tctgatgtaa ttttcctctc tctaaggatg ataggaggct tgtagctcaa
     1801 tatcgttttc ccatttctag tttgctgctg gtaatgtatc tgcatctaac actctgtgta
     1861 agctgttgtg tctttgcaca gtttatgcag tgctgctttg ccaaataaaa taaaaagcaa
     1921 aagaagcatt ggtggccttt gaatctggaa gaacacaggt ctaggtccag atgctgccca
     1981 cggccatggg gaaacgttgt attctgctgc tgtgtcttgt gatagcctag gaggtaaatc
     2041 tgcgtagcat ttgtccactc tctgtggctg catttcttca ggaatggaga ctgtgtgttc
     2101 attgtttctc tctgccttcc tagcccaggg cttgctattt aatagctctg tagtaatgct
     2161 cagtgagtga ggggtgagtg tgtatcgggg aagaatgggt gagcataaac ataccgaagt
     2221 ccacgtctca tctgtacata gattcattac cttaataact gtgaatgtct gggaaggcca
     2281 tggcccttgg aggatcttat ttaaatatgc ttttatattt tctaagaaga ctttagtttt
     2341 tatgaggatt ctagaatttg agagaagatt ataagccccc agtaaatggg gctaagctaa
     2401 gcgaagcctt ccctctgtga cagggcccat ggttctgaca agaaagttct tagtgtccga
     2461 gaagcccttc cccactcttc atgtcataca cacttcactg gacaagtgtc tcttcgtggc
     2521 ttaggtctca gtcccaggaa actagcatta ccgtggtcct gcagacttga aaatcttacc
     2581 cagctttggt ttgctgctgg ttgttgcttt ggaatcctgt cagtgacccc tggagtccag
     2641 gatgcatggt catttgaagt atgtgtaaag tcaaatgttc actcaggctt taacgcaaat
     2701 aaattcttaa actta
//
